scholarly journals Study the Inhibitory effect of Rose and blossom bitter orange flowers extracts against different types of bacteria isolated from the oral cavity

2014 ◽  
Vol 11 (2) ◽  
pp. 795-802
Author(s):  
Baghdad Science Journal
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Cold Water ◽  
Inhibitory Effect ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Bitter Orange ◽  
Wide Range ◽  
Different Types ◽  
Amoxicillin Clavulanic Acid

The study in duded isolation and identification of microbial isolates from oral cavity to 10 volunteers, diagnosed within the three groups: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp. and Candida albicans . The sensitivity test of all isolates bacteria Streptococcus spp. , S. aureus and S. epidermidis showed high resistance to Ampicillin(100)%,followed Methicillin (88.88)% and Amoxicillin / clavulanic acid(77.77)%, while the resistance for each of Vancomycin and Amoxicillin were (66.66)%, and the resistance to Erythromycin and Pencillin (55.55)% to each of them. The results showed less resistance to Trimethoprim (22.22)% and Cefalotine (11.11)% of all bacteria isolate. Investigation of the presence of active compounds in each of the hot and cold (water and alcoholic) extracts flowers Rose and flowers blossom bitter orange ( new preparatory 2012) (Alkaloids, Quartet alkaloids, sugars, Saponine, Flavones and comarins ) was carried out includes. While it was all kinds of extracts does not contain resins. The results showed the presence of active compounds (Sugars, Flavones and comarins) in old extracts that preparation after a year (2011). PH values of the plant extracts hot and cold (water and alcoholic) for each of orange flowers blossom and Rose flowers (Old and new preparatory) with a wide range ranging between (3.6 -6.4). All extracts hot and cold (alcoholic) (new preparatory 2012) showed most effective towards Streptococcus spp., S. epidermidis and Candida albicans, while the extracts show weak effective against S. aureus. Hot alcohol extracts of Rose (old preparatory 2011) showed most effective towards S. aureus and S. epidermidis only, while the other extracts of old perpetration show weak effective against Streptococcus spp. and C. albicans.

scholarly journals THE LEVEL OF PLASTICITY OF THE PHASES IN NON-METALLIC INCLUSIONS WITH A COMPLEX STRUCTURE

2021 ◽  
pp. 19-25
Author(s):  
S.I. GUBENKO
Keyword(s):  
Complex Structure ◽  
Cold Deformation ◽  
Inhibitory Effect ◽  
Practical Significance ◽  
Plastic Behavior ◽  
Metallic Inclusions ◽  
Wide Range ◽  
Different Types ◽  
Deformation Ability ◽  
Composition Structure

Purpose. It is necessary to study of the effect of heterophase inclusions on the technological ductility of steels for various purposes. The goal of the work was to study of the nature and level of plasticity of multiphase inclusions in steels under conditions of hot and cold deformation. Methods. Comprehensive methods for the study of heterophase non-metallic inclusions (metallographic, petrographic, X-ray microanalysis methods) were used. Results. Plastic phases in multiphase inclusions of different types under conditions of hot and cold deformation of steels were investigated. It is shown that each type of multiphase inclusions, which are microcomposite formations in steels, is characterized by its laws of development of deformation processes, which are determined by their chemical and phase composition, structure, deformation ability of the phases of inclusions. Scientific novelty. Peculiarities of plastic behavior of multiphase inclusions of different types are established. The inhibitory effect of non-deformable phases of inclusions on the deformability of plastic phases in a wide range of steel deformation temperatures is established. Peculiarities of the nature of plasticity of multiphase inclusions having different compositions and structure are discussed. Practical significance. Using the results obtained will allow developing technologies for producing steels with regulated content and types of multiphase non-metallic inclusions, which will significantly increase their technological plasticity, as well as prevent the formation of various kinds of defects during the processing of steels by pressure.

scholarly journals Green Anaconda (Eunectes murinus) with Bacterial Clinical Stomatitis

2019 ◽  
Vol 47 ◽  
Author(s):  
Nathana Beatriz Martins ◽  
Lucas Arthur Ricardo ◽  
André Luiz Quagliatto ◽  
Rafael Rocha De Souza ◽  
Thaís De Almeida Moreira ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Predisposing Factors ◽  
Resistant Bacteria ◽  
Microbiological Analysis ◽  
Isolation And Identification ◽  
Bacterial Diseases ◽  
Normal Microbiota ◽  
Wide Range ◽  
Enterococcus Spp ◽  
Free Animal

Background: Stomatitis is an infectious disease common in serpents and responsible for high mortality rates. It is characterized by the infection of the oral mucosa and neighboring tissues, related to the opportunistic character of bacteria present in the normal microbiota, pathogenic in stressful situations. Few studies have described the profile of sensibility of these agents in serpents of the Brazilian fauna. Therefore, this study has aimed at describing the isolation and identification of the infectious agents involved in the clinic stomatitis in a specimen of green anaconda (Eunectes murinus), and the profile of susceptibility to antimicrobial agents.Case: The serpent has been rescued in an urban environment, without previous records and featured erosive injuries in its oral cavity, with the presence of secretion. In a clinical evaluation, it has been assessed that the specimen had erosive injuries in its oral cavity, with hyperemic points in its mucosa and serous secretion. Then the specimen went through a collection of the secretion from its oral cavity for microbiological analysis. Typical colonies of Enterococcus, Citrobacter and Enterobacter were identified by the colony morphology and their typical odor. The results of these tests were able to confirm and identify the Citrobacter freundii, Enterobacter spp. and Enterococcus spp. The profile of sensibility to antimicrobials of the isolated microorganisms has been determined through the method of diffusion in the disk of Kirby-Bauer. There was not any sensitive antimicrobial drug for the three agents.Discussion: Immunodepression, malnutrition, and temperatures and humidity outside the animal thermal comfort zone, are predisposing factors for the development of bacterial diseases in reptiles. Little information about pathogen agents affecting constrictor serpents in their native area is available. Most reports on stomatitis in serpents approach cases occurred in captive animals, differently from what has been presented in this case, which is about a free animal rescued when invading an urban area. The bacteria isolated from the oral cavity of the serpent here reported belong to the normal microbiota of the oral cavity of these animals. Notwithstanding, these bacteria may become pathogenic in certain circumstances. Conditions of undernutrition, stress and oral trauma are considered as predisposing factors to the occurrence of stomatitis in serpents, what can be correlated to the occurrence of the disease in this case. The gram-negative agents causing bacterial diseases in serpents are generally resistant to medicines of the most common spectrum used in the clinical routine of wild animals. This way, veterinarians often deal with these diseases in reptiles empirically, using a wide range of antibiotics. This practice might result in the development of resistant bacterial stumps, what stands out due to the potential that resistant bacteria have to generate infections and zoonoses in humans.

scholarly journals Biological effect of Lawsonia inermis plant

2017 ◽  
Vol 27 (4) ◽  
Author(s):  
Buthaina Abdul-Hamza H. ALzubedy ◽  
Shaymaa O. M. ALhamdany ◽  
Raghad K. M. ALkazaly ◽  
Masya A. A. ALzubedy ◽  
Zenia AbdulKareem ◽  
...  
Keyword(s):  
Plant Extract ◽  
Cold Water ◽  
Water Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Nano Particles ◽  
Alcoholic Extract ◽  
Lawsonia Inermis ◽  
Different Types ◽  
Antimicrobial Effectiveness

This study was compared the antimicrobial effectiveness of Lawsonia inermis plant extract (alcoholic, aqueous and silver nanoparticles) on the growth of different types of Gve+ bacteria and Gve- bacteria at concentration 80 mg /L. It was noted that the ( alcoholic, hot and cold water) extract have highest impact recorded in the growth of bacteria S.aurous with inhibition zone (26mm) followed by the bacteria P.aueroginosa at inhibition zone reached (22mm) and finally the yeast C. albicans at inhibition zone reached (17mm), while silver nano-particles of the plant extract the most influence on the bacteria, it gave the highest Inhibition area reached (30mm) on the growth of the S. aurous, followed by the inhibition area of P.aueroginosa reached (26mm) then Strep. pyogein (25mm), and finally the yeast C. albicans reached (18 mm). With regard to the complex (alcoholic extract and antibiotic Amoxylein) its effects has varied upon microorganisms, there is a synergistic effect on the growth of the yeast C. albicans at inhibition zone (30mm) while it has been observed inhibitory effect of this complex on the growth of p. aeroginosa with inhibition zone amounted to (15mm), but did not show any significant impact on the growth of both bacteria (K. pneumonia, E.coli Strep. Pyogein& P. aeroginosa,), while the (alcoholic extract and antibiotic Erythromycin) showed inhibitory and synergistically effect on each of the bacteria (E.coli, S. aurous, yeast C. albicans and P. aeroginosa) respectively. According to obtained results the antibiotic Erythromycin shows higher inhibitory effect than antibiotic Amoxylein. It has also been investigated the lower values of absorbance at the wavelength of 260 nm with increasing the concentration of crude alcoholic extract, this reveled the ability of henna extract in the removal of free radicals liberated from photolysis of hydrogen peroxide compound .

scholarly journals Efficacy of Toothpaste in Reducing Micro-Flora Isolated from Toothbrush

2018 ◽  
Vol 13 (2) ◽  
pp. 71-78 ◽  
Author(s):  
Bikram Gautam ◽  
Sabi Pokhrel ◽  
Sagar Aryal ◽  
Anup Basnet
Keyword(s):  
Oral Cavity ◽  
Bacterial Infections ◽  
Inhibitory Effect ◽  
Microbial Load ◽  
Plate Count ◽  
Lactobacillus Species ◽  
Klebsiella Pneumonia ◽  
Local Market ◽  
Wide Range ◽  
Gram Negative Organisms

Oral cavity is the easiest site of entry of microorganisms during breathing, eating, drinking and brushing which can lead to several bacterial infections in oral cavity, pharynx, larynx, gastrointestinal tracts etc. Toothbrushes commonly used to maintain oral health and prevent dental disease; but unfortunately, how keeping the toothbrush is neglected. A wide range of chemicals have been added to toothpastes in order to produce a direct inhibitory effect on plaque formation and kill microorganisms. The aims of the study were to investigate the relationship between toothbrush keeping place, its microbial content determine the type of micro-flora present in toothbrush kept in different locations and to determine efficacy of toothpaste in reducing micro-flora isolated from toothbrush. Used toothbrushes were taken from 21 individuals. 2 (1 herbal and 1 regular) toothpastes were selected for the study and were collected from local market. Standard pour plate method and plate count method were performed to determine the reduction of microbial load. Out of 21 toothbrushes, 19 (90.48%) were found to be growth positive and 2 (9.52%) were growth negative. Common Gram positive organisms isolates includes Lactobacillus species (20%), Bacillus subtilis (5%), Bacillus megaterium (5%), Stahpylococcus aureus (25%), Staphylococcus epidermidis (10%), Micrococcus species (10%) and Gram negative organisms isolated include Citrobacter freundii (5%), Pseudomonas aeruginosa (5%), Proteus mirabilis (5%), Enterobacter species (5%) and Klebsiella pneumonia (5%). Toothbrushes kept in the toilet/bathroom showed contamination with pathogens. Toothpaste T1 was found to be better at reducing microbial load compared to T2. Toilet/bathroom is the worst place for keeping toothbrushes. Toothpastes have their own patent, specialty and were found to be effective against the microorganisms. Synergistic interactions between the principal components of toothpaste can be considered to be a vital part of their efficacy.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 71-78

scholarly journals Preliminary study on the inhibitory effect of seaweed Gracilaria verrucosa extract on biofilm formation of Candida albicans cultured from the saliva of a smoker

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 684
Author(s):  
Zaki Mubarak ◽  
Adintya Humaira ◽  
Basri A. Gani ◽  
Zainal A. Muchlisin
Keyword(s):  
Candida Albicans ◽  
Immune System ◽  
Antifungal Activity ◽  
Oral Cavity ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Multiple Factors ◽  
Preliminary Study

Background: Candida albicans is an opportunistic fungus that infects the oral cavity. Increases in colony numbers of C. albicans can be caused by multiple factors, such as smoking, a weakened immune system, taking antibiotics and with immune-compromised individuals. Smoking can increase the virulence factor of C. albicans and make it stronger. One of the virulence factors of C. albicans is the biofilm it forms. The C. albicans biofilm makes it more tolerant to extracts of the seaweed Gracilaria verrucosa, which has antifungal activity. The objective of the study was to examine the ability of the G. verrucosa extracts to inhibit the formation of biofilm by C. albicans obtained from the saliva of smoker. Methods: A total of six concentrations of G. verrucosa (6.25, 12.5, 25, 50, 75 and 100%) were tested in this study. The positive control was fluconazole 0.31 µg/ml C. albicans was taken from the saliva of one smoker in Faculty of Dentistry, Syiah Kuala University. The total amount of biofilm was assessed using an ELISA reader. The data were subjected to Kruskal-Wallis test at a significance limit of p<0.05. Results: The seaweed extract has three bio-active compounds: steroids, terpenoid, and tannins. The results showed that the inhibitory activity of seaweed on C. albicans biofilm formation increases as its concentration increases. The highest effectiveness was recorded at a seaweed concentration of 100% at 48 h of exposure. Conclusions: The optimal inhibition of the C. albicans biofilm formation was recorded at the concentration of 100% G. verrucosa after 48 hours of exposure.

scholarly journals Isolation and Identification of Candida from the Oral Cavity

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Smitha Byadarahally Raju ◽  
Shashanka Rajappa
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Hiv Infection ◽  
Antifungal Agents ◽  
Isolation And Identification ◽  
Diagnosis And Management ◽  
Oral Candidosis ◽  
Reliable Methods

Various techniques are available for the isolation of Candida within the oral cavity. Such methods play an important role in the diagnosis and management of oral candidosis. The growing importance of Candida is in part related to the emergence of HIV infection and the more widespread use of immunosuppressive chemotherapy. Along with the Candida albicans there has been a greater recognition of the importance of the nonalbicans Candida species in oral candidosis. Identification of infecting strains of Candida is important because isolates of Candida species differ widely, both in their ability to cause infection and also in their susceptibility to antifungal agents. Thus this review provides an overview of the reliable methods of candidal isolation and identification of isolates from the oral cavity.

scholarly journals Evaluation of antimicrobial activity of synthesized 9H-alkylcarbazole and 10H-alkylphenothiazine derivatives on the cells of Salmonella enterica ser. Typhimurium, Saccharomyces cerevisiae, and Candida albicans

Biologija ◽  
2020 ◽  
Vol 66 (2) ◽  
Author(s):  
Simona Sutkuvienė ◽  
Sandra Sakalauskaitė ◽  
Neringa Kuliešienė ◽  
Lina Ragelienė ◽  
Rimantas Daugelavičius
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Albicans ◽  
Salmonella Enterica ◽  
Alkyl Chain ◽  
Efflux Pump ◽  
Inhibitory Effect ◽  
Phenothiazine Derivatives ◽  
Pharmacological Activities ◽  
Alkyl Chains ◽  
Wide Range

10H-substituted phenothiazine and 9H-substituted carbazole derivatives are important because of a very wide range of applications and especially in medical chemistry due to their pharmacological activities. In this study, we synthesized 9H-alkylcarbazole and 10H-alkylphenothiazine derivatives with various lengths of alkyl chains and evaluated their antimicrobial and efflux inhibiting activities on the cells of Salmonella enterica ser. Typhimurium, Saccharomyces cerevisiae, and Candida albicans. Results of our study revealed that an increased length of alkyl chains of the carbazoles increased the accumulation of efflux indicator tetraphenylphosphonium (TPP+) ions. Cells of S. enterica efflux mutant ΔTolC had a considerable susceptibility to the synthesized compounds. The compounds exerted synergy with fluconazole against S. cerevisiae yeast. Efflux pump mutant ΔPdr5 was hypersensitive to the investigated carbazole and phenothiazine derivatives. The inhibitory effect of the compounds with a shorter alkyl chain (10-methyl-10H-phenothiazine and 9-methyl-9H-carbazole) was the highest for Candida albicans cells.

scholarly journals Uji Anti Jamur Ekstrak Buah Mengkudu (Morinda citrifolia L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 92-102
Author(s):  
Ririen Hardani ◽  
I Kadek Adi Krisna ◽  
Baharuddin Hamzah ◽  
Muhammad Fakhrul Hardani
Keyword(s):  
Candida Albicans ◽  
Measurement Data ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Fruit Extract ◽  
Different Types ◽  
The Media ◽  
Inhibitory Power

Noni is a plant that has the potential to be used as traditional medicinal ingredients. This study aims to determine the inhibition of noni extracts (Morinda citrifolia L.) in inhibiting the growth of the fungus Candida albicans. This type of research is experimental. A total of 150 grams of dried noni fruit that has been mashed, macerated with three different types of solvents namely aquades, acetone and n-hexane to obtain thick extract. Preparation of the fungus suspension was Candida albicans made by mixing the media Nutrient Broth with 5 ose test fungus that were equated with turbidity of a standard MC. Farland. The fungus suspension made was implanted on the Sabouroud Dextrose agar to solidify and divided into 7 sterile petri dishes. Testing the inhibition of fungi using the method of wells. Each cup is made of 1 hole diameter of 3 cm in the center. Tests were carried out by adding non-solvent aquades fruit extract into the 1 cup, acetone noni fruit extract into the cup 2, n-hexane solvent noni fruit extract into the cup 3, for 3 other plates added with three solvents as negative control, while for 1 cup added to the cup fungal infection drug is nystatin as a positive control, each as much as 1 mL. Measurement data were analyzed using the formula of the percentage of inhibition. Inhibitory power of noni fruit extract using distilled water is 3.55%, noni fruit extract using acetone solvent is 50.15%, noni fruit extract using n-hexane solvent that is equal to 38.83%. negative control of aquades, acetone and n-hexane solvent has no inhibitory power while positive control of nystatin is 9.73%. Noni fruit extract using acetone solvent has the strongest inhibitory ability. It can be concluded that the noni fruit extract has fungal inhibitory effect on the growth of the fungus Candida albicans

Surface microhardness of different restorative materials exposed to candida albicans biofilm isolated from the oral cavity of hiv-infected children

2019 ◽  
Vol 4 (1) ◽  
pp. 87-91
Author(s):  
Leandro COSTA ◽  
Larissa SOARES-SILVA ◽  
Paulini Malfei De C. COSTA ◽  
Adrielle MANGABEIRA ◽  
Maristela PORTELA ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Surface Microhardness ◽  
Restorative Materials

Research of matrix metalloproteinases in therapeutic dentistry

2020 ◽  
pp. 51-57
Author(s):  
L.I. Shalamay ◽  
E.Y. Nechai ◽  
A.I. Sakerina ◽  
A.G. Gabdullin
Keyword(s):  
Matrix Metalloproteinases ◽  
Oral Cavity ◽  
Catalytic Domain ◽  
Different Types ◽  
Dental Diseases ◽  
Classification Function

This article the properties of matrix metalloproteinases, their classification, function and role in the development of dental diseases are presented. The analysis of matrix metalloproteinases has been performed, which proves their important role in the physiological and pathological processes of the oral cavity. The possibility of using matrix metalloproteinases as part of one of the methods for diagnosing diseases and using them to evaluate the effectiveness of treatment has been considered. The structure of different types of metalloproteinases, as well as a model of the catalytic domain of the MMP-8 molecule has been presented.

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