scholarly journals Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Sunil L. Baldania ◽  
Ankit B. Parmar ◽  
Kashyap K. Bhatt ◽  
Dimal A. Shah ◽  
Usmangani K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Correlation Coefficients ◽  
Retention Factor ◽  
Olmesartan Medoxomil ◽  
Pharmaceutical Formulations ◽  
Simultaneous Estimation ◽  
Phase Detection ◽  
Metoprolol Succinate ◽  
Tlc Plates

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method is described for simultaneous analysis of metoprolol succinate and olmesartan medoxomil in pharmaceutical formulations. The separations were achieved on prepared TLC plates precoated with silica gel G 60 F254 and the plates were developed with methanol-ethyl acetate-toluene-glacial acetic acid 2.5 : 3 : 4.5 : 0.3%v/v/vas mobile phase. Detection and evaluation of chromatograms were performed densitometrically at 224 nm. The retention factor of metoprolol succinate and olmesartan medoxomil was 0.25 and 0.70, respectively. The linear range was 1000–2250 ηg per spot for metoprolol succinate and 800–1800 ηg per spot for olmesartan medoxomil and the correlation coefficients (r) were 0.993 and 0.999, respectively. The method was validated for linearity and range, specificity, precision, accuracy, and robustness, and the results were found to be within the acceptance criteria. The method was successfully applied to the analysis of drugs in their capsule dosage form.

ESTIMATION OF METFORMIN HYDROCHLORIDE AND TENELIGLIPTIN IN PHARMACEUTICAL FORMULATION BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (10) ◽  
pp. 34-39
Author(s):  
V. B Patel ◽  
◽  
J. S. Patel ◽  
D. A Shah
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Retention Factor ◽  
Metformin Hydrochloride ◽  
Phase Detection ◽  
Chromatographic Separations ◽  
Ich Guidelines ◽  
Tlc Plates ◽  
Tablet Dosage ◽  
Layer Chromatography

A new, accurate and precise high performance thin layer chromatographic (HPTLC) method has been developed for simultaneous analysis of metformin hydrochloride and teneligliptin in tablet formulation. The chromatographic separations were achieved on TLC plates precoated with silica gel G60 F254 and the plates were developed with 0.5% ammonium acetate-n propanol-ammonia (4.5:5:0.5, v/v/v) as mobile phase. Detection and evaluation of chromatograms were performed densitometrically at 252 nm. The retention factor of metformin hydrochloride and teneligliptin was found to be 0.2 and 0.63, respectively. The method was found to be linear in the range of 100-1200 ng per spot and 200-2400 ng per spot for metformin and teneligliptin, respectively. The method was validated with respect to precision, accuracy, specificity and robustness as per ICH guidelines Q2 (R1) and the results were found to be within the acceptance criteria. The method was successfully applied for the analysis of drugs in combined tablet dosage form.

DEVELOPMENT AND VALIDATION OF RP - HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL AND METOPROLOL SUCCINATE IN BULK AND DOSAGE FORM

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (01) ◽  
pp. 50-53
Author(s):  
S. B. Jadhav ◽  
◽  
V. V Kunjir ◽  
S. K. Kupkar ◽  
P. D. Chaudhari
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Olmesartan Medoxomil ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Metoprolol Succinate ◽  
Resolution Factor ◽  
Tablet Dosage ◽  
Liquid Chromatographic

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of olmesartan medoxomil and metoprolol succinate in tablet dosage form. A Phenomenex-C18, 5 mcm column 250 mm 4.6 mm in gradient mode, with mobile phase containing phosphate buffer, acetonitrile and methanol in proportion of 65:35 v/v, pH 5.5 adjusted with orthophosphoric acid were used. The flow rate was 1 mL/min, and effluent was monitored at 254 nm.The retention time of olmesartan medoxomil and metoprolol succinate were 8.02 and 5.36 min respectively, and the resolution factor was 9.0. Linearity range for olmesartan medoxomil and metoprolol succinate was 5-30 mcg mL-1 and 5-70 mcg mL-1 respectively. The proposed method is accurate, precise,specific and rapid for simultaneous estimation of olmesartan medoxomil and metoprolol succinate in tablet dosage form.

scholarly journals Validated high performance thin layer chromatographic analysis of leaves and flower extracts of

2022 ◽  
Vol 8 (4) ◽  
pp. 270-279
Author(s):  
Anup K Chakraborty ◽  
Harshita Tripathi ◽  
Sarita Karole ◽  
Kavita R Loksh
Keyword(s):  
Thin Layer ◽  
Silica Gel ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Chemotherapeutic Agents ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tlc Plates ◽  
Thin Layer Chromatographic Analysis ◽  
Hptlc Method

is a significant therapeutic plant has a place with family apocynaceae contains in excess of 70 distinct sorts of chemotherapeutic agents and alkaloids which help in treating different illnesses. For the most part, it is known as Vincarosea, Ammocallisrosea and Lochnerarosea. There are numerous or more than 400 alkaloids present in plant, which are used as flavor, agrochemicals, pharmaceuticals, fragrance, ingredients, food addictives, and pesticides. To develop a validated high performance thin layer chromatographic method for the analysis of leaves and flower extracts of Sample solutions were applied onto the plates with automatic TLC sampler Linomat V (Camag, Muttenz, Switzerland) and were controlled by WinCATS software. Plates were developed in 10 x 10cm twin trough glass chamber (Camag, Muttenz, Switzerland). A CAMAG TLC scanner was used for scanning the TLC plates. Pre-coated silica gel aluminium plates 60F254. For vincristine, simultaneous estimation of vincristine was performed by HPTLC on a silica gel plate using toluene-methanol-diethylamine (8.75: 0.75: 0.5, v/v/v) as the mobile phase. The method was validated as per the ICH guidelines. The Rf value was found to be 0.76 for flower and 0.80 for leaves at 250 nm which shows the presence of vincristin in . In this research paper, a validated HPTLC Method has been developed for the analysis of leaves and flower extracts

scholarly journals SIMULTANEOUS ESTIMATION OF CURCUMINOIDS, PIPERINE, AND GALLIC ACID IN AN AYURVEDIC FORMULATION BY VALIDATED HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD

2016 ◽  
pp. 117
Author(s):  
Niraj Yashvantrai Vyas ◽  
Sejal Patel
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
High Performance ◽  
Retention Factor ◽  
Simultaneous Estimation ◽  
Phase Plate ◽  
Analytical Work ◽  
Ambient Room Temperature ◽  
Marker Compounds ◽  
Hptlc Method

<p>ABSTRACT<br />Objective: The present study was proposed to quantitatively estimate the amount of three marker compounds; curcuminoids, piperine, and gallic<br />acid in a multicomponent ayurvedic formulation using high-performance thin layer chromatographic (HPTLC) method for routine analytical work.<br />Methods: TLC separation was performed on silica gel 60 F<br /> plates using toluene:ethyl acetate:formic acid:methanol (5.6:2.2:1.2:1.0 v/v/v/v) as<br />mobile phase. Plate was developed by to a distance of 90 mm at ambient room temperature with 20 minutes saturation time. Densitometric analysis<br />was performed at 327 nm. Method was validated as per International Conference on Harmonization Q2 (R1) guideline also.<br />254<br />Results: Piperine, curcuminoids, and gallic acid were separated on TLC at retention factor values of 0.71, 0.61, and 0.29, respectively. The described<br />method was linear over the range of 300-700 ng/spot, 100-300 ng/spot, and 250-550 ng/spot, respectively, for curcuminoids, piperine, and gallic<br />acid. The accuracy of the method was assessed by recovery studies and was found to be 101.71%, 99.67%, and 99.59% for curcuminoids, piperine,<br />and gallic acid, respectively. The amount of curcuminoids, piperine, and gallic acid in the ayurvedic formulation was found to be 3.99% w/w, 1.9%<br />w/w, and 0.8% w/w, respectively, when analyzed quantitatively by developed validated HPTLC method.<br />Conclusion: The method can be used as a tool for quality control of herbal formulation.<br />Keywords: Curcuminoids, Piperine, Gallic acid, High-performance thin layer chromatographic.</p>

scholarly journals A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations

2011 ◽  
Vol 8 (s1) ◽  
pp. S41-S46
Author(s):  
Prafulla Kumar Sahu ◽  
M. Mathrusri Annapurna ◽  
Dillipkumar Sahoo
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Aqueous Acetic Acid ◽  
Linear Calibration ◽  
Acceptance Range ◽  
Liquid Chromatographic

This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines.

DEVELOPMENT OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND ORNIDAZOLE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (05) ◽  
pp. 36-43
Author(s):  
N. R Dighade ◽  
◽  
M. D Shende ◽  
A. V Kasture
Keyword(s):  
Quality Control ◽  
High Resolution ◽  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Hptlc Method ◽  
Routine Quality Control

A simple and accurate high performance thin layer chromatographic (HPLTC) method has been developed and validated as per ICH guidelines for estimations of Ciprofloxacin (CP) and Ornidazole (ORN) in combined dosage form. The mobile phase was acetonitrile: toluene: water and triethylamine (5.5:1.8:1.5:1.6 V/V) was found to be best which gave high resolution with Rf 0.16 and 0.84 for ciprofloxacin and ornidazole respectively. The linearity of ciprofloxacin and ornidazole was found to be in the range of 0.4 to 0.8 µg/mL and 0.4 to 0.8 µg/mL, respectively. The coefficient of correlation (r2 ) was found to be greater than 0.989 for both the components by this method. The tablet analyses result (n = 5) were found to be > 100.84 % by HPTLC for both the components. The proposed method was found to be simple, accurate and suitable for routine quality control of marketed formulations containing these drugs.

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ABACAVIR SULPHATE AND LAMIVUDINE IN TABLET DOSAGE FORM BY RP-HPLC

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 12-16
Author(s):  
S Vidyadhara ◽  
◽  
L. S Reddyvalam ◽  
T. Koduri ◽  
P. K. Borra ◽  
...  
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Method Development ◽  
Correlation Coefficients ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Liquid Chromatographic

A simple, accurate, precise high-performance liquid chromatographic (HPLC) method has been developed and validated for the simultaneous determination of abacavir sulphate (ABA) and lamivudine (LAM) in combined dosage form. Separation was performed on a C18 column [Agilent ODS UG 5 column, 250 mm x 4.5 mm], with methanol: water (50:50 V/V) isocratic elution using a flow rate of 1mL/min. Good sensitivity was observed with UV detection at 277 nm. After method development, the interference of other active compounds and excipients, repeatability and linearity, were investigated. Retention times of LAM and ABA were found to be 3.3 and 6.3 min, respectively. The method was validated over the range from 2.5-12.5 μg/mL for LAM and 5-25 μg/mL for ABA with correlation coefficients of 0.9997 and 0.9996, respectively. This method was shown to be accurate, robust, selective, linear, and repeatable and can be successfully employed in routine quality control for the simultaneous analysis of ABA and LAM in tablets.

Sign in / Sign up

Export Citation Format

Share Document