scholarly journals Evidence for Genetic Similarity of Vegetative Compatibility Groupings in Sclerotinia homoeocarpa

2014 ◽  
Vol 30 (4) ◽  
pp. 384-396 ◽  
Author(s):  
Seog Won Chang ◽  
Young-Ki Jo ◽  
Taehyun Chang ◽  
Geunhwa Jung
Keyword(s):  
Genetic Similarity ◽  
Vegetative Compatibility ◽  
Sclerotinia Homoeocarpa

scholarly journals Genetic Diversity of Sclerotinia homoeocarpa Isolates from Turfgrasses from Various Regions in North America

Plant Disease ◽  
2004 ◽  
Vol 88 (11) ◽  
pp. 1269-1276 ◽  
Author(s):  
G. Viji ◽  
W. Uddin ◽  
N. R. O'Neill ◽  
S. Mischke ◽  
J. A. Saunders
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Creeping Bentgrass ◽  
The United States ◽  
Vegetative Compatibility ◽  
Major Group ◽  
Golf Courses ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Geographical Regions

Sixty-seven isolates of Sclerotinia homoeocarpa, causing dollar spot disease in creeping bentgrass, annual bluegrass, Bermudagrass, and perennial ryegrass turf, collected from 23 golf courses in various geographical regions of the United States and Canada between 1972 and 2001, were characterized by vegetative compatibility, genetic diversity, and pathogenicity. Eleven vegetative compatibility groups (VCGs A to K) were identified among the isolates tested in this study, and five of them (VCGs G to K) were new. VCG B was the most predominant group, typifying 33 isolates (51%) tested. S. homoeocarpa isolates collected from golf courses in Pennsylvania belonged to seven VCGs (A, B, E, F, G, I, and K), whereas three groups were observed in those collected from New York (B, E, and G) and New Jersey (E, H, and I). Two isolates, one each from Pennsylvania and Canada, were incompatible when paired with the tester isolates in all possible combinations, and did not fall into any known VCG. An isolate collected from Canada was compatible with tester isolates from two VCGs (C and D). Genetic analyses using amplified fragment length polymorphism (AFLP) showed the presence of two genetically distinct groups, designated as major group and the minor group. The major group included 36 isolates collected from various golf courses in the United States and Canada. Two isolates collected from bermudagrass in Florida formed a separate cluster, the minor group. Isolates that belonged to the major group were further divided into two subgroups (1 and 2). Subgroup 1 consisted of all the isolates that belonged to VCGs A, E, G, H, and I. Three of the four isolates that belonged to VCG K also were clustered with isolates of subgroup 1. Subgroup 2 consisted of all the isolates from VCG B, and one each from VCGs F and K. Pathogenicity assays on Penncross creeping bentgrass showed significant differences (P = 0.05) in virulence among the isolates. Overall, a relationship between virulence and VCGs was observed, in which certain virulence groups corresponded to specific VCGs; however, such a relationship was not observed between virulence and AFLPs. Close similarity among isolates of S. homoeocarpa collected from different locations in the United States and Canada suggests that isolates of the same genotype could be involved in outbreaks of dollar spot epidemics at multiple locations.

Response of Bentgrass Cultivars to Sclerotinia homoeocarpa Isolates Representing 10 Vegetative Compatibility Groups

Crop Science ◽  
2006 ◽  
Vol 46 (3) ◽  
pp. 1237-1244 ◽  
Author(s):  
Nanda Chakraborty ◽  
Taehyun Chang ◽  
Michael D. Casler ◽  
Geunhwa Jung
Keyword(s):  
Vegetative Compatibility ◽  
Sclerotinia Homoeocarpa ◽  
Vegetative Compatibility Groups

scholarly journals Reassessment of Vegetative Compatibility of Sclerotinia homoeocarpa Using Nitrate-Nonutilizing Mutants

2008 ◽  
Vol 98 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Y.-K. Jo ◽  
S. W. Chang ◽  
J. Rees ◽  
G. Jung
Keyword(s):  
Minimal Medium ◽  
The United States ◽  
Vegetative Compatibility ◽  
Complete Medium ◽  
Sclerotinia Homoeocarpa ◽  
Hyphal Fusion ◽  
Vegetative Compatibility Groups ◽  
Nit Mutants ◽  
Mutant Phenotypes ◽  
First Time

Nitrate-nonutilizing (nit) mutants were recovered for the first time from 21 isolates of Sclerotinia homoeocarpa collected in the United States. Mutants were selected from shredded mycelium of each isolate when cultured on water agar medium amended with 4% (wt/vol) potassium chlorate. The mutants could be classified into three phenotypes: nit1, nit3, and NitM, based on their growth on minimal medium (Czapek solution agar) supplemented with NaNO2 or hypoxanthine. Complementary heterokaryons were observed in pairings between different phenotypes of nit mutants derived from compatible isolates, but not in self-fusions or pairings between incompatible isolates. The vigor of prototrophic growth varied with isolates and mutant phenotypes. Strong and continuous heterokaryons, as well as weak and spontaneous ones, formed depending on pairings of nit mutants. Stable heterokaryons between compatible isolates, but apoptotic reactions between incompatible isolates, were observed immediately after hyphal fusion under the epifluorescence microscope. The 21 isolates used in this study, which were previously assigned into 11 different vegetative compatibility groups (VCGs) based on the formation of a barrage zone at the contact site of paired isolates on complete medium (potato dextrose agar), were regrouped into five VCGs based on heterokaryon formation between nit mutants on minimal medium.

scholarly journals Genetic Analysis of Fungicide-Resistant Sclerotinia homoeocarpa Isolates from Tennessee and Northern Mississippi

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 83-90 ◽  
Author(s):  
R. E. DeVries ◽  
R. N. Trigiano ◽  
M. T. Windham ◽  
A. S. Windham ◽  
J. C. Sorochan ◽  
...  
Keyword(s):  
Fungicide Resistance ◽  
Molecular Data ◽  
Vegetative Compatibility ◽  
Internal Transcribed Spacers ◽  
Sclerotinia Homoeocarpa ◽  
Vegetative Compatibility Group ◽  
Arithmetic Means ◽  
Putting Greens ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group

Sclerotinia homoeocarpa is the causal agent of dollar spot disease that reduces the uniformity and aesthetic value of golf putting greens. Fungicide-resistant isolates of S. homoeocarpa were collected from putting greens at 10 locations across Tennessee and northern Mississippi. Genetic diversity among the 60 isolates was investigated using vegetative compatibility, conserved gene sequences, and amplified fragment length polymorphism (AFLP). Six tester strains were paired with Tennessee and northern Mississippi isolates on potato dextrose agar. Some of the 60 isolates were delineated into vegetative compatibility groups, but fungicide resistance could not be associated with a particular vegetative compatibility group. Genetic similarities of isolates at the vegetative compatibility level could be attributed to founder effects. Sequencing the regions of CAD, EF1-α, β-tubulin, and internal transcribed spacers revealed 100% homology among isolates. Capillary gel electrophoresis and analysis of AFLP fragments indicated 86 to 100% similarity between the isolates. Vegetative compatibility and molecular data indicate that the populations of the pathogen are clonal. Isolates did not cluster according to fungicide resistance during unweighted pair group with arithmetic means analysis, but did appear to cluster according to vegetative compatibility group and location. Although associations could not be made between molecular markers and fungicide resistance, links between vegetative compatibility and AFLP markers may provide a foundation from which other studies could be performed.

scholarly journals Vegetative Compatibility and Seasonal Variation Among Isolates of Sclerotinia homoeocarpa

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 377-381 ◽  
Author(s):  
J. F. Powell ◽  
J. M. Vargas
Keyword(s):  
United States ◽  
Seasonal Variation ◽  
Single Species ◽  
Vegetative Compatibility ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Vegetative Compatibility Groups ◽  
Seasonal Epidemics ◽  
Northern United States ◽  
Multiple Species

Dollar spot of amenity turf, caused by Sclerotinia homoeocarpa, occurs in two seasonal epidemics in the northern United States, one from May to late July and a second from mid-August through October. It is not known whether these seasonal epidemics are the result of multiple species or due to seasonal variation within a single species. Isolates of S. homoeocarpa were collected from dollar spot lesions obtained from golf courses in Michigan, Illinois, and Wisconsin. Vegetative compatibility reactions between isolates identified six vegetative compatibility groups (VCGs) among more than 1,300 isolates collected from the eight locations. Most VCGs were present throughout the season, but one was generally recovered only in the late epidemic. Sequences of the nuclear ribosomal internal transcribed spacer 1 (ITS1) were identical among VCGs, indicating that the VCGs represent a single species. The results of this study suggest that the seasonal dollar spot epidemics observed in the northern United States are caused by a single species.

Phytotoxic tetranorditerpenoids from the fungus Sclerotinia homoeocarpa, causal agent of dollar spot in turfgrass

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
HMT Herath ◽  
WHMW Herath ◽  
SO Duke ◽  
M Tomaso-Peterson ◽  
NPD Nanayakkara
Keyword(s):  
Causal Agent ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot

CONSEQUENCES OF “HOLSTEINIZATION” OF UKRAINIAN BLACK-PIED DAIRY BREED BY GENE BOLA-DRB3.2

2017 ◽  
Vol 54 ◽  
pp. 146-156
Author(s):  
T. M. Suprovych ◽  
M. P. Suprovych ◽  
R. V. Kolinchuk
Keyword(s):  
Genetic Distance ◽  
Milk Production ◽  
Genetic Similarity ◽  
Restriction Analysis ◽  
Allelic Polymorphism ◽  
Milk Productivity ◽  
High Productivity ◽  
Black And White ◽  
Holstein Breed ◽  
Specific Pcr

Introduction. The main direction of increasing the productivity of milk is to increase the proportion of heredity of the Holstein breed in the genotype of cows. Industrial breeds in Ukraine are improving due to the increase in the Holstein inheritance in the genotype of cows. The "holsteinization" of the most widespread domestic Black-and-White diary breed is intensively conducted. Currently, the percentage of heredity from Holstein is 90% or more. The negative effect of "holsteinization" appeared in reducing the resistance of animals to diseases that led to the spread of necrobacterial pathology. The control of the spread of necrobacteriosis can be based on genetic markers. Important markers can be the allele of the BoLA-DRB3.2 gene responsible for the formation of adaptive immunity. Due to the ambiguity of the results of "holsteinization", the following tasks were solved: To study the genetic structure of the herd for the BoLA-DRB3.2 gene at the beginning of the "holsteinization" and now. To compare the detected genetic structures with the alleles spectrum of North American Holstein and identify quantitative and qualitative changes in the structure of the herd genotype. To determine the effect of "holsteinization" on the dynamics of milk production and the state of morbidity by necrobacteriosis. Materials and methods of research. Comparison of alleles of population of the Ukrainian Black-Pied Dairy (UBPD) breed and Holstein breed was conducted to detect the consequences of "holsteinization" on milk yield and incidence of necrobacteriosis. The data of the allelic polymorphism of the BoLA-DRB3.2 gene of the UBPD10 (2010, n = 162), UBPD15 (2015, n = 114) and two Holstein populations of the USA and Canada were collected. The allelic spectrum was determined by the PCR-RFLP method. The amplification of the BoLA-DRB3.2 gene was performed using 2-step PCR with the use of primers HLO-30, HLO-31 and HLO-32 and allele-specific PCR. Restriction analysis was performed with endonuclease RsaI, HaeIII, BstYI (XhoII). Restriction fragments were separated by electrophoresis in 4% agarose gel. Counting of allele frequencies was performed taking into account the number of homozygotes and heterozygotes found for the corresponding alleles. To determine the phylogenetic relationships between the populations of the studied herds, genetic distance and genetic similarity were determined by the M. Nei method. Individual dairy productivity of cows was estimated for all lactation (regardless of its duration). Average milk yields were determined as the total volume of milk produced divided by the number of dairy cows. Results and discussion. The breeding measures carried out led to the accumulation of alleles characteristic of the Holstein breed. For Holstein, there are eight alleles with a frequency of more than 4%. It is alleles *03, *07, *08, *11, *16, *22, *23, *24. A high degree of consolidation of weighty alleles can be outlined. In total they occupy 84,6% of allele spectrum of the population. Consolidation of such alleles in the herd of the Ukrainian Black-and-White diary breed is much lower - only 52.2%, although it increased by 6.2% over 5 years. Alleles *10, *13 and *28 are "weighty" for the Ukrainian Black-and-White diary breed, but they are almost non-existent in Holsteins. The genetic similarity of the herd UBPD15 and Holstein increased by ΔI = 0,085, and the genetic distance between the herds of the UBPD increased by ΔD = 0,085 for 5 years. The comparison of the allele spectrum of Holstein and the Ukrainian Black-and-White diary breed shows both the accumulation and the elimination of alleles associated with high productivity. The largest consolidation is typical for alleles *24 (+ 6.75%) and *16 (+ 4.65%). The frequency of "milk" alleles *22 and *08 decreased, respectively, by 4.14 and 1.27%. Alleys, which cause low milk productivity, have the following dynamics: * 23 + 2.53%, *11 – 0.67 and *28 – 0.26. The accumulation of alleles *16 and *23 (7.18%) was found that are associated with predisposition to necrobacteriosis and elimination of *03 and *22 alleles (4.75%) that influence on this disease. Conclusions. It is determined that the role of alleles characteristic for Holstein is increasing in the the Ukrainian Black-and-White diary herd. Breeding measures for holsteinization are conducted in the right direction. There is accumulation of alleles associated with high milk productivity and predisposition to necrobacteriosis. It positively affects the growth of milk production and negatively affects the incidence of necrobacteriosis.

Comparative analysis of genetic background in eight near-isogenic wheat lines with different H genes conferring resistance to Hessian fly

Genome ◽  
2011 ◽  
Vol 54 (1) ◽  
pp. 81-89 ◽  
Author(s):  
S. S. Xu ◽  
C. G. Chu ◽  
M. O. Harris ◽  
C. E. Williams
Keyword(s):  
Genetic Background ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Hessian Fly ◽  
Pedigree Analysis ◽  
Recurrent Parent ◽  
Linkage Drag ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
H Gene

Near-isogenic lines (NILs) are useful for plant genetic and genomic studies. However, the strength of conclusions from such studies depends on the similarity of the NILs’ genetic backgrounds. In this study, we investigated the genetic similarity for a set of NILs developed in the 1990s to study gene-for-gene interactions between wheat ( Triticum aestivum L.) and the Hessian fly ( Mayetiola destructor (Say)), an important pest of wheat. Each of the eight NILs carries a single H resistance gene and was created by successive backcrossing for two to six generations to susceptible T. aestivum ‘Newton’. We generated 256 target region amplification polymorphism (TRAP) markers and used them to calculate genetic similarity, expressed by the Nei and Li (NL) coefficient. Six of the NILs (H3, H5, H6, H9, H11, and H13) had the highly uniform genetic background of Newton, with NL coefficients from 0.97 to 0.99. However, genotypes with H10 or H12 were less similar to Newton, with NL coefficients of 0.86 and 0.93, respectively. Cluster analysis based on NL coefficients and pedigree analysis showed that the genetic similarity between each of the NILs and Newton was affected by both the number of backcrosses and the genetic similarity between Newton and the H gene donors. We thus generated an equation to predict the number of required backcrosses, given varying similarity of donor and recurrent parent. We also investigated whether the genetic residues of the donor parents that remained in the NILs were related to linkage drag. By using a complete set of ‘Chinese Spring’ nullisomic-tetrasomic lines, one third of the TRAP markers that showed polymorphism between the NILs and Newton were assigned to a specific chromosome. All of the assigned markers were located on chromosomes other than the chromosome carrying the H gene, suggesting that the genetic residues detected in this study were not due to linkage drag. Results will aid in the development and use of near-isogenic lines for studies of the functional genomics of wheat.

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