Comparative studies between six taxa of Amaranthaceae based on their effects on some pathogenic bacterial isolates and morphological characters.

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.

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Lignin-Degrading Microorganisms from Organic Soils

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2021/v33i430458 ◽

2021 ◽

pp. 78-85

Author(s):

Yalavarthi Nagaraju ◽

. Mahadevaswamy ◽

S. B. Gowder ◽

S. Triveni

Keyword(s):

Lignin Peroxidase ◽

Manganese Peroxidase ◽

Higher Plants ◽

Dye Decolorization ◽

Morphological Characters ◽

Organic Soils ◽

Bacterial Isolates ◽

Biochemical Tests ◽

Fungal Isolates

The most prevalent aromatic polymer in nature is lignin, produced by higher plants and thought to make up 30-35 percent of the non-fossil organic carbon on the planet. Lignin hydrolyzing enzymes such as lignin peroxidase, laccase, manganese peroxidase, and others produce a variety of aromatic monomers, including ferulic and vanillic acids. However, very little research has been done on the role of microbes in lignin degradation. In the present work, we have isolated 25 ligninolytic bacteria and 25 ligninolytic fungi from organic soils of Koppal, Raichur districts of Karnataka. The bacterial isolates were identified as Pseudomonas putida, Bacillus subtilis, based on biochemical tests, and fungi were identified as Aspergillus niger, Trichoderma viridae, Phanerochaete chrysosporium and Pleurotus ostreatus based on morphological characters. The ligninolytic activity of bacterial isolates was high when compared to fungal isolates. All the isolates produced detectable amounts of lignin peroxidase, manganese peroxidase, and laccase under in vitro conditions. In dye decolorization test, fungal isolates KGST-1, KGST-2, and KKSP could decolorize Ramazol Brilliant Blue R and Congo red.

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SKRINING BAKTERI BERPOTENSI PENDEGRADASI POLIETILEN OXO-DEGRADABLE DARI TANAH GAMBUT DI SEKITAR TPA KUALA DUA RASAU JAYA

Jurnal Protobiont ◽

10.26418/protobiont.v8i3.36680 ◽

2019 ◽

Vol 8 (3) ◽

Author(s):

Nadhifah Rizqi Firdaus ◽

Rahmawati Rahmawati ◽

Riyandi Riyandi

Keyword(s):

Incubation Time ◽

Peat Soil ◽

Morphological Characters ◽

Disposal Site ◽

Bacterial Isolates ◽

Plate Method ◽

Enrichment Broth ◽

West Kalimantan ◽

Agar Media ◽

Incubation Method

The use of polyoethylene degradable polyethylene in Indonesia, especially in West Kalimantan, is high enough to potentially cause environmental pollution. One way to resolve pollution caused by polyethylene oxo-degradable plastic waste is to utilize isolates from the peat soil around the Kuala Dua Final Disposal Site, Rasau Jaya, which is able to degrade oxo-degradable polyethylene. This research aims to determine the bacterial genera that can to be expected to degrade Oxo-Degradable Polyethylene. Samples were taken from peat soil with the incubation method of oxo-degradable polyethylene for 2 weeks, 4 weeks and 6 weeks. Isolation use the pour plate method with Enrichment Broth Media and Enrichment Agar Media. The isolation results obtained 9 (nine) bacterial isolates which are distinguished based on incubation time and morphological characters.

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