scholarly journals The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus

2021 ◽  
Vol 10 (1) ◽  
pp. 1-13
Author(s):  
Dwi N Susilowati ◽  
Sri Rahayuningsih ◽  
Indah Sofiana ◽  
Nani Radiastuti
Keyword(s):  
Culture Method ◽  
Morphological Characters ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Inhibitory Zone ◽  
Myristica Fragrans ◽  
Hemolysis Test ◽  
The Difference ◽  
Myristica Fragrans Houtt ◽  
Index Value

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.       

scholarly journals EKSPLORASI DAN IDENTIFIKASI CENDAWAN ANTAGONIS TERHADAP JAMUR AKAR PUTIH (Rigidoporus lignosus) PADA TANAMAN PALA (Myristica fragrans Houtt)

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nana Ariska ◽  
Lola Adres Yanti ◽  
Chairudin Chairudin
Keyword(s):  
Economic Value ◽  
Culture Method ◽  
Dilution Method ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Pests And Diseases ◽  
Myristica Fragrans ◽  
Rigidoporus Lignosus ◽  
Myristica Fragrans Houtt

Nutmeg (Myristica fragrans Houtt) is a multipurpose plantation commodity crop that almost all parts of the plant have strategic economic value to be cultivated. In Indonesia there are the largest nutmeg commodity centers, including the Maluku islands, North Sulawesi and Aceh. The biggest problem with nutmeg plants is the attack of pests and diseases. One of the most severe diseases in nutmeg is white root mushroom (Rigidoporus lignosus). This study aims to 1) determine the fungus of white root fungus antagonists (Rigodoporus lignosus) from the Aceh nutmeg rhizosphere (Myristica fragrans Houtt), 2) identify the content of antibiotic fungus JAP antagonist compounds in Aceh nutmeg plants. The study was conducted by taking soil samples from nutmeg plants at three nutmeg plantations in South Aceh. The antagonist fungus isolation activity uses a dilution method. Isolation of JAP from infected roots using direct planting method. Furthermore, it is identified macroscopically and microscopically. The antagonist test was carried out using the dual culture method with RAL. From the rhizosphere in the nutmeg area, 5 candidate antagonist isolates were obtained, having high in vitro antagonism ability to the pathogen R. lignosus. Identification results showed that isolates 1, 2 and 3 were Trichoderma spp, and isolates 4 and 5 were Gliocladium virens. Keywords: In vitro, the content of antibiotic compounds, Aceh nutmeg, Rigidoporus lignosus, antagonistic test

scholarly journals ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH

2020 ◽  
Vol 14 (2) ◽  
pp. 178-186
Author(s):  
Lisa Novita Arios ◽  
Dwi Suryanto . ◽  
Kiki Nurtjahja . ◽  
Erman Munir .
Keyword(s):  
Endophytic Bacteria ◽  
Culture Method ◽  
Dry Weight ◽  
Dual Culture ◽  
Seedling Height ◽  
Bacterial Isolates ◽  
Peanut Seed ◽  
Number Of Leaves

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings.   A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method.  In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.

scholarly journals Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea)

2021 ◽  
Vol 6 (1) ◽  
pp. 60618
Author(s):  
Indah Juwita Sari ◽  
I Nyoman Pugeg Aryantha
Keyword(s):  
Bacterial Isolate ◽  
Culture Method ◽  
Gene Analysis ◽  
Dual Culture ◽  
Volvariella Volvacea ◽  
Bacterial Isolates ◽  
Base Pairs ◽  
Mycelium Growth ◽  
Screening And Identification ◽  
Growth Promoting

This research aimed to identify the indigenous Mushroom Growth Promoting Bacteria (MGPB) bacteria that can increase the growth of Volvariella volvacea. The research began by isolating indigenous MGPB from planting media of straw mushrooms in Karawang, Indonesia. The screening was performed to select bacterial isolates that can promote the highest growth of mushrooms by dual culture method on PDA media. There were 10 of the 58 highest bacterial isolates that have a positive effect on the vegetative growth of mushrooms. The 23K bacterial isolate was the most significant increase in mycelium growth compared to other isolates and bacteria-induced controls. A bacterial isolate 25K by gene analysis was identified by 16S rRNA (518F primer (5’- CCA-GCA-GCC-GCG-GTA-ATA-CG -3’) and 800R primer (5’- TAC-CAG-GGT-ATC-TAA-TCC -3’). The result from gene analysis shows that there are ~1550 base pairs products. BLAST analysis and phylogenetic tree adjustment results show that the closest diversity of this bacterial isolate 25K is Bacillus thuringiensis serovar konkukian str. 92-27 (equality value = 99%).

scholarly journals Pengaruh Lama Penyulingan terhadap Rendemen dan Mutu Minyak Atsiri pada Biji Pala (Myristica fragrans Houtt)

2018 ◽  
Vol 11 (1) ◽  
pp. 50-58
Author(s):  
Fadillah Ramadhanti Rangkuti ◽  
Raida Agustina ◽  
Mustaqimah Mustaqimah ◽  
Mustafril Mustafril
Keyword(s):  
Refractive Index ◽  
Essential Oil ◽  
High Refractive Index ◽  
The Other ◽  
A Value ◽  
Myristica Fragrans ◽  
Weight Value ◽  
Myristica Fragrans Houtt ◽  
Index Value

Abstrak. Pala merupakan salah satu tanaman rempah yang menghasilkan minyak atsiri. Penelitian ini bertujuan untuk mengetahui pengaruh lama penyulingan terhadap rendemen dan mutu minyak biji pala. Variasi perlakuan yang digunakan adalah lama penyulingan yaitu 0-3 jam, 3-6 jam, 6-9 jam, 9-12 jam, 12-15 jam, 15-18 jam, 18-21 jam, 21-24 jam, 24-27 jam, dan 27-30 jam. Analisis karakteristik yang dilakukan meliputi rendemen, bobot jenis, indeks bias dan kelarutan dalam alkohol. Hasil penelitian yang telah dilakukan didapat rendemen minyak pala yang bervariasi, tergantung dari lama penyulingan. Pada jam ke 3 menghasilkan rendemen yang lebih besar dibandingkan dengan jam yang lain, dimana rendemen minyak pala pada jam ke 3 didapat sebesar 5,58 % sedangkan rendemen yang paling rendah terdapat pada jam ke 30 dengan hasil sebesar 0.12%. Berdasarkan hasil perhitungan rendemen kumulatif yang didapat dari range waktu 0-3, 3-6, 6-9, 9-12, 12-15, 15-18, 18-21, 21-24, 24-27, dan 27-30 adalah sebesar 10, 48 %. Nilai bobot jenis minyak pala yang diperoleh dari hasil penyulingan pada jam ke 24 menunjukkan angka yang lebih tinggi dibandingkan hasil yang lainnya dengan nilai 0,936 dan tidak memenuhi standar SNI. Sedangkan yang paling rendah terdapat pada jam ke 3 dengan nilai 0.872 yang juga tidak sesuai dengan standar SNI. Penyulingan dengan nilai bobot jenis yang sesuai dengan standar SNI terdapat pada jam ke 6 dengan nilai 0,902. Nilai indeks bias yang tinggi didapatkan pada jam ke 12- 15, 18-21, 21-24 dan 24- 27 dengan nilai 1,496 dan memenuhi standar SNI sedangkan pada jam ke 3-6 diperoleh nilai indeks bias sebesar 1,436 dan tidak memenuhi standar SNI. Tingkat kelarutan dalam alkohol 90% minyak pala yang dihasilkan dari 6 jam sampai 30 jam adalah sama, yaitu jernih yang diuji dengan perbandingan 1:3 dan sudah sesuai standar SNI sedangkan pada ke 3 jam hasilnya adalah opalisensi yaitu tidak keruh dan tidak jernih. Effect of Old Distillation on Rendemen and Quality of Essential Oils on Nutmeg (Myristica fragrans Houtt) Abstract. Nutmeg is one of the herbs that produce essential oil. This study aims to determine the effect of distillation on rendemen and quality of nutmeg oil. The variation of treatment used was the distillation time of 0-3 hours, 3-6 hours, 6-9 hours, 9-12 hours, 12-15 hours, 15-18 hours, 18-21 hours, 21-24 hours, 24- 27 hours, and 27-30 hours. Characteristic analyzes performed include rendemen, species weight, refractive index and solubility in alcohol. The results of the research have been obtained the yield of various nutmeg oil, depending on the length of distillation. At third hours it produces a higher yield compared to the other hour, where the yield of nutmeg oil at third hourswas5.58%, while the lowest yield was at 30thhours with a yield of 0.12%. Based on the results of cumulative rendemen calculations obtained from the range of time 0-3, 3-6, 6-9, 9-12, 12-15, 15-18, 18-21, 21-24, 24-27, and 27-30 was 10, 48%. The weight value of nutmeg oil obtained from the distillation at 24 hours indicates a higher rate than the other results with a value of 0.936 and didn't meet the SNI standard. While the lowest was at third hours with a value of 0.872 which was also not in accordance with SNI standards. Distillation with value of weight of type according to SNI standard is at 6th hours with value 0,902. High refractive index values were obtained at 12 to 15, 18-21, 21-24 and 24-27 hours with a value of 1.496 and met the SNI standard while at 3-6 hours the refractive index value was 1.436 and did not meet the SNI standard. The solubility rate in 90% alcohol of nutmeg oil produced from 6th hours to 30th hours is the same, that was clear tested with ratio of 1: 3 and was accordance with SNI standard while in 3 hours the result is opalisensi that is not cloudy and not clear.

scholarly journals Efficacy of Some Rhizosphere Microorganisms in Controlling Fusarium Wilt of Tomato (Lycopersicon esculentum)

2014 ◽  
Vol 11 (2) ◽  
pp. 96-103
Author(s):  
AM Sarker ◽  
N Sultana ◽  
MR Islam ◽  
M Rashid ◽  
ZR Bhuian
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Trichoderma Harzianum ◽  
Rhizosphere Soil ◽  
Culture Method ◽  
Inhibition Zone ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Diagnostic Laboratory ◽  
Rhizosphere Microorganisms

An experiment was conducted at the Disease Diagnostic Laboratory and Net House of Plant Pathology Department, Sher-e-Bangla Agricultural University, Dhaka during July 2011 to June 2012 to investigate the efficacy of some antagonistic rhizosphere microorganisms against Fusarium oxysporum f. sp. lycopersici causing Fusarium wilt of tomato. Probable 20 antagonistic bacterial isolates and one antagonistic fungal isolate (Trichoderma harzianum) from rhizosphere soil were screened out against F. oxysporum f. sp. lycopersici. Out of 20 bacterial isolates, only four (Iso-1, Iso-2, Iso-3 and Iso-4) were found effective in dual culture method. Out of all antagonists, Trichoderma harzianum produced the highest inhibition zone (75.75 %), while the lowest inhibition zone (28.39 %) was produced by Iso- 2. In blotter method, the highest prevalence of Fusarium oxysporum was observed in control (11.25%), while the lowest was in Iso-1 (0.58%) treated seeds. DOI: http://dx.doi.org/10.3329/agric.v11i2.17494 The Agriculturists 2013; 11(2) 96-103

scholarly journals Eksplorasi dan Karakterisasi Khamir dan Bakteri sebagai Agens Antagonis terhadap Penyebab Penyakit Blas pada Padi

2018 ◽  
Vol 14 (3) ◽  
pp. 83
Author(s):  
Jauharoh Maknunah ◽  
Meity Suradji Sinaga
Keyword(s):  
Agar Medium ◽  
Blast Disease ◽  
Dual Culture ◽  
Rice Seed ◽  
Bacterial Isolates ◽  
Chitinolytic Activity ◽  
Candida Sp ◽  
Activity Test ◽  
Hemolysis Test ◽  
Planting Method

Exploration of Yeasts and Bacteria as Antagonist Agent of Rice Blast PathogenBlast disease (Pyricularia oryzae) is a major disease of rice that causes 61% yield losses. An alternative way to control the disease is applying antagonistic agents, both yeasts and bacteria. This study was aimed to obtain yeast and bacterial isolates that can be used as antagonistic agents to effectively control blast disease. Yeasts and bacteria were isolated from the leaves of Digitaria sp., Panicum sp., and Pennisetum sp.. The isolates of yeasts and bacteria were screened following hypersensitive test on tobacco leaves, direct planting method test on ‘Ciherang’ rice seed, and hemolysis test on blood agar medium. The isolates were also further assayed for their antagonism through dual culture test, volatile compound test, hyperparasitism test, and chitinolytic activity test. Three isolates of yeast, i.e. Cryptococcus sp., Rhodotorula sp., and Candida sp., and two bacterial isolates, i.e. Bacillus sp. and isolate PPY were identified as potential antagonists of P. oryzae.

scholarly journals THE POTENTIAL OF ENDOPHYTIC FUNGI AS BIOCONTROL AND PHOSPATE SOLUBILIZATION AGENT IN Capsicum anuum

2020 ◽  
Vol 3 (3) ◽  
pp. 16-19
Author(s):  
Dwi Ningsih Susilowati
Keyword(s):  
Growth Inhibition ◽  
Endophytic Fungi ◽  
Phytophthora Capsici ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Colletotrichum Acutatum ◽  
Limiting Factor ◽  
Dual Culture ◽  
Fusarium Sp ◽  
Index Value

The productivity of chili (Capsicum annuum) in Indonesia is currently very low. Some factors that influenced it including the presence of pathogenic microorganisms which lead to the low availability of phosphate in the soil. This condition become a limiting factor for plant growth and production. Endophytic fungi can be used as antagonistic agents in inhibiting pathogenic fungi and to increase the efficiency of phosphate solubilization known as phospholytic fungi. This study aimed to find antagonistic agents from endophytic fungi that can suppress the growth of pathogenic fungi and test the ability of endophytic fungi to dissolve phosphate. Fungi isolates used were BB-Biogen collection isolates, consisting of 42 endophytic fungi isolates, and 3 pathogenic fungi isolates (Fusarium sp., Colletotrichum acutatum, Phytophthora capsici) on chili plants (C. annuum). The antagonism test was carried out using the dual culture method in the Potato Dextrose Agar (PDA) medium for 5 days incubation at temperature (± 28 ° C). The parameters measured were based on the formation of inhibition zones and the calculation of the percentage of growth inhibition of fungi isolates. The test results obtained 7 representative fungi isolates (RIVA4, RIVA5, MIVD2, Aspergillus niger, Cladosporium sp., Cladosporium oxysporum, and Chaetomium globosum). Based on the calculation of the percentage of growth inhibition, fungi isolates with RIVA5 code have a higher potential in inhibiting the growth of all three pathogenic fungi. Calculation of the percentage of endophytic fungi inhibition of RIVA5 were 70.3% (Fusarium sp.), 63.3% (C. acutatum), and 60% (P. capsici). Phosphate test was carried out by the cork borer method in pikovskaya medium for 4 days incubation at 27-28 ° C. The parameters measured were based on the formation of clear zones around the colony. There were 4 endophytic fungi isolates (MIVA4, MIVF7, Aspergillus sydowii, and  A. niger) formed a clear zone around the colony, which indicates the presence of phospholytic activity. Based on the calculation of the phospholytic index, A. niger isolates have a high phosphate solubility index value of 5.

Phytochemical and Pharmacological Activity of Myristica fragrans Houtt (Myristicaceae)

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Md Arifur Rahman Chowdhury ◽  
Manirujjaman . ◽  
Md Mazedul Haq
Keyword(s):  
Acetic Acid ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Writhing Test ◽  
Myristica Fragrans ◽  
Myristica Fragrans Houtt

Objective: Myristica fragrans Houtt commonly used as traditional medicine for alleviating of various disorders. The purpose of our study was to map out the in vitro antioxidant property and in vivo anti-hyperglycemic and analgesic effect of the methanolic extract of Myristica fragrans Houtt. (Seed and mace) (Myristicaceae) on Swiss albino mice. Methods: The processed powder of Myristica fragrans Houtt (seed and mace) were subjected to methanolic extraction by soxhlet filtration methods, and the desiccated extract was used for screening of antioxidant by DPPH free radical scavenging assessment as well as total phenolic content by using folin-ciocalteu reagent.Anti-hyperglycemic effect and analgesic action tested through alloxan induced antidiabetics test and acetic acid-tempted writhing test on mice. Results: In DPPH free radical scavenging assessment, free radicals neutralization expressed as % of inhibition 49.69±0.06% also by IC50 values as 68.43 µg/ml surmise middle level of antioxidant property. The total phenolic content expressed as 186.25 mg/g equivalent of gallic acid indicates, active phenolic content. Oral administration of 200 and 400 mg/kg of extract dose and reference drug vildagliptin (50 mg/kg) for the duration of the 4-day study period, and initiated % of inhibition the blood glucose level measured as 22.48%, 44.78% and 62.02% regard as the significant anti-hyperglycemic properties. The analgesic activity was investigated by using the acetic acid-induced writhing test in mice, at the dose of 200 mg/kg body and 400mg/kg weight, and resulting 50.4% and 68.10% correspondingly, which was considerably significant with a standard drug. Conclusion: The present study suggests that methanolic extract of seed and mace of Myristica fragrans Houtt can manage moderate oxidative stress as well as perform the painkilling action. Besides, prolong medication may enhance the new dimension of anti-hyperglycemic activity.

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