DETEKSI Liberibacter spp. PADA JERUK SIAM BERGEJALA KLOROSIS DISERTAI BERCAK HITAM DENGAN Polymerase Chain Reaction

Liberibacter bacteria are one of the pathogenic bacteria in citrus plants. Siam Citrus Plant in Setapok Village, Singkawang City, suspected to be infected with Liberibacter spp. bacteria by showing patterns of symptoms of chlorosis accompanied by patches. This study aims to detect Liberibacter spp. on Siam Citrus leaves with symptomatic chlorosis with patches. Leaf sampling uses the roaming method in one of the citrus plantations in Setapok Village, Singkawang City. Detection of Liberibacter spp. done by PCR (Polymerase Chain Reaction) technique. Detection of bacterial DNA through several stages namely, isolation of bacterial plasmid DNA, 16S rDNA gene amplification with a thermocycler machine, and visualization using a set of electrophoresis devices. Electrophoresis results show the presence of DNA band lines, with a size of around 1100 bp for OI1 and OI2c primers, and DNA bands of 703 bp for A2 and J5 specifics. The results showed that there were Liberibacter spp. in the symptomatic chlorosis of Siam Citrus leaves accompanied by patches.

JENIS-JENIS SEMUT ARBOREAL (HYMENOPTERA: FORMICIDAE) DI KAWASAN KAMPUS UNIVERSITAS TANJUNGPURA DENGAN TIGA METODE KOLEKSI

Ants as insects occupy various habitats and ecological niches. Universitas Tanjungpura is an area with variable vegetation physiognomies that has the potential to have a lot of ant microhabitat. This study aims to determine the most effective method for collecting arboreal ants (Hymenoptera: Formicidae) found in the campus area of Universitas Tanjungpura. The study was conducted from August 2018 to February 2019 at ten locations based on their environmental condition. Ants were collected in three different methods: hand collecting, bait traps, and yellow pan traps. There were 39 species of Formicidae collectedfromall locations. These results were obtained by hand collecting (32 species), bait traps (32 species), and yellow pan traps (4 species). Hand collecting and bait traps are the most effective method for collecting arboreal ants in Universitas Tanjungpura.

INVENTARISASI ZINGIBERACEAE DI KAWASAN HUTAN TEMBAWANG DESA SUMBER KARYA KECAMATAN TERIAK KABUPATEN BENGKAYANG

Zingiberaceae is the family of flowering plants commonly known by the people of Indonesia as the Jahejahean. This plant is widely used for many things such as spices, medicine, ornamental plants, cosmetic ingredients, beverage ingredients, hair tonic, etc. The aim of the study is to know Zingiberaceae species diversity in Tembawang Forest, Sumber Karya Village, Teriak District, Bengkayang City, West Kalimantan. The method of the research used exploration with observation in three Tembawang Forest at Sumber Karya Village. The results showed that there were seven species of Zingiberaceae namely E. coccinea, E. elatior, E. nasuta, G. atrosanguinea, H. reticulata, Z. multibracteatum, and Z. zerumbet.

PEMANFAATAN TUMBUHAN OBAT OLEH MASYARAKAT SUKU DAYAK KENDAWANGAN DI DESA RANGKUNG KECAMATAN MARAU KABUPATEN KETAPANG

Dayak Kendawangan tribe is a sub-tribe of Dayak which is spread around Kendawangan River in Rangkung Village, Marau District, Ketapang Regency. Dayak Kendawangan tribe still uses plants as traditional medicine. The purpose of this study was to determine the types of medicinal plants utilized by Kendawangan Dayak people in Rangkung Village. This research was conducted in October to December 2018. The method used was snowball with 11 respondents and conducted interviews with respondents to obtain information on traditional medicines and analyzed using qualitative and quantitative analysis. The results showed there were 52 species from 33 families, the most widely used family is Zingiberaceae (9,62 %). Part of a widely used plant is the leaves (42,18 %), while the way of processing and the use of medicinal plant is in boiled (59,61 %) and drunk (50,94 %). The most uptake of medicinal plants in the yard (47,82 %) and forest (39,13 %). The highest frequency of citation (FIC) value is Psidium guajava (54,5 %). Plant part value (PPV) and interspecific use value (IUV) of plants used in traditional medicine of Kendawangan Dayak tribe are 0.5-1 and 0.3-2, respectively.

EFEKTIVITAS ANTIMIKROBA BUAH LAKUM (Cayratia trifolia [L.] DOMIN) TERHADAP PERTUMBUHAN BAKTERI Streptococcus sp. (L.10.3)

Streptococcus sp. (L.10.3) is one of the bacteria that can cause dental plaque. This study was aimed to determine the antimicrobial activity of the fraction of methanol fraction of fruit against Streptococcus sp. (L.10.3). This study used a Completely Randomized Design (CRD), 3 levels of concentrations of fraction methanol fraction 0.3 g/mL, 0.6 g/mL and 0.9 g/mL and 2 positive control treatments of amoxicillin and negative control using distilled water with three times repeated. Testing the antibacterial activity using the Kirby-Bauer method using disc paper. The results of the study showed the average diameter of inhibition zones of Streptococcus sp. (L.10.3) in the 24th hour incubation ranged from 7.70-10.72 mm and in the 48th hour incubation ranged from 8.70-10.37 mm. A concentration of 0.9 g/mL is an effective concentration with a moderate inhibition response category. Giving fraction of methanol fraction of fruit at the lowest concentration of 0.3 g/mL is able to provide a moderate inhibition response with the bacteriocidal antibacterial category.

PERTUMBUHAN KEDELAI (Glycine max [L.] Merrill) VARIETAS ANJASMORO DENGAN PEMBERIAN BIOURIN KAMBING (Capra aegagrus hircus)

Soybean (Glycine max [L.] Merrill) Anjasmoro variety is one of the superior soybean varieties widely cultivated in West Kalimantan. The growth of soybean can be increased by applying liquid organic fertilizer namely goat biourine. The purpose of this study was to determine the goat biourine quality and the effect of the application of goat biourine on the growth of Anjasmoro variety soybean plants. This study uses a Completely Randomized Design (CRD) consisting of five treatments, namely without biourine/control, 100 mL/L biourine, 200 mL/L biourine, 300 biourine mL/L and 400 mL/L biourine. Each treatment was repeated 5 times to obtain 25 experimental units. The analysis of goat biourine showed the value of C-Organic 2.03%, pH 4.83, N 0.72%, P 0.004% and K 0.234%. The results showed that apply of goat biourine had a significant effect on plant height, number of leaves, shoot wet of weight, shoot dry of weight, number of branches, number of root nodules and the number of effective root nodules, but it had not a significant effect on root wet of weight and root dry of weight.

PENGARUH KOTORAN AYAM DAN MIKORIZA Glomus aggregatum TERHADAP PERTUMBUHAN KEDELAI (Glycine max) PADA TANAH GAMBUT

Peat soil in West Kalimantan has been contained low of nutrient level so it affects the growth of soybeans. One effort to increase the availability of nutrients in peat soils is by using mycorrhiza and chicken manure fertilizer. This research aims to determine the growth of soybeans by administering chicken manure and mycorrhiza on peat soils. The research was conducted from January to April 2019. The research design used was a Completely Randomized Design Factorial consisting of 2 factors with 4 replications. The first factor is mycorrhiza fungi: M1 (0 gr/polybag), M2 (5 gr/polybag), M3 (10 gr/polybag), M4 (15 gr/polybag) and the second factor is chicken manure fertilizer: P1 (0 tons/ha) , P2 (5 tons/ha), P3 (10 tons/ha), P4 (15 tons/ha). The results showed that the single chicken manure application significantly affected all observed parameters with 15 tons/ha chicken manure fertilizer. Mycorrhiza fungi just a significantly effect on fresh weight and dry weight. Also, the combination of mycorrhiza fungi and chicken manure fertilizer just a significantly affected wet weight and dry weight with combination 5 g mycorrhiza and 5 tons/ha of chicken manure fertilizer.

JENIS BURUNG DI KAMPUS UNIVERSITAS TANJUNGPURA

Quality degradation, modification, and habitat loss are significant threats to bird species. The natural habitat of birds has been modified into residential land and facilities to meet the needs of human life as happened at Tanjungpura University. This study aims to determine of birds species at Tanjungpura University. Observations were carried out from January to March 2019. The method used in collecting the data from bird was “Encounter rates” which was conducted in the morning starting at 6 – 9 am and at 3 - 6 pm. The data obtained were analyzed with the formula of simple abundance scale and frequency of attendance. The birds found at Universitas Tanjungpura are 28 species classified into 23 genera, 17 families, and seven orders. Birds found to have an abundance order scale are classified into abundant, general, frequent and, unusual categories. Birds included in the abundant category are Collocalia fuciphaga and Passer montanus. The types of bird foods at Tanjungpura University consist of frugivore, insectivore, granivore, herbivore, carnivore, piscivore, omnivore, molluscivore, and nectarivore. The value of attendance frequency have range between 10-100%. The bird species with highest frequency of attendance’s value is Passer montanus, Pycnonotus aurigaster, Pycnonotus goiavier, Collocalia fuciphaga, and Anthreptes malacensis.

INVENTARISASI JAMUR MAKROSKOPIS FILUM ASCOMYCOTA DI KAWASAN UNIVERSITAS TANJUNGPURA PONTIANAK KALIMANTAN BARAT

Ascomycota is a microscopic fungi and a fraction of macroscopic, unicellular and multicellular fungi. The research of Ascomycota phylum macroscopic fungi inventory research aims to determine the type macroscopic fungi of Ascomycota phylum members in Tanjungpura University Area, Pontianak, West Borneo. The sample was taken by using Cruise Method and identified by matching morphological characteristics of the specimens with the identification book. Ascomycota identification of macroscopic fungi samples of Ascomycota phylum based on morphological characteristics with the identification books. The results study found have 3 macroscopic fungi species consisting of 2 classes, 2 orders, 2 families, and 2 genera. In addition, Ascomycota found in Tanjungpura University Area, Pontianak, West Borneo, grow mostly on the substrate originated from dead trees.

KARAKTERISASI Bacillus spp. DAN Fusarium sp. DARI TANAMAN LADA (Piper nigrum L.) DI DESA JAGA

Pepper (Piper nigrum L.) is one of the farm crop which become commodities in West Kalimantan. This research aimed to determine the morphological characteristic of Bacillus bacteria from rhizosphere of pepper and Fusarium fungi with symptoms of yellow disease. Sampling was done in pepper farm crops, Sungai Jaga Village, Bengkayang Regency. Isolation and identification were implemented at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Tanjungpura University, Pontianak. The result of isolation and identification showed 4 bacterial isolates belong to genus Bacillus, namely Bacillus sp. BRF1, Bacillus sp. BRF2, Bacillus sp. BRF3 and Bacillus sp. BRF4 and one isolate of Fusarium sp. JDF from pepper with symptom of yellow desease.