Moderately thermostable phage Φ11 Cro repressor has novel DNA-binding capacity and physicochemical properties

Invasiveness of trophoblast and choriocarcinoma cells is in part mediated via leukemia inhibitory factor- (LIF-) induced activation of signal transducer and activator of transcription 3 (STAT3). The regulation of STAT3 phosphorylation at its ser727 binding site, possible crosstalk with intracellular MAPK signaling, and their functional implications are the object of the present investigation. JEG-3 choriocarcinoma cells were cultured in presence/absence of LIF and the specific ERK1/2 inhibitor (U0126). Phosphorylation of signaling molecules (p-STAT3 (ser727 and tyr705) and p-ERK1/2 (thr 202/tyr 204)) was assessed per Western blot. Immunocytochemistry confirmed results, but also pinpointed the location of phosphorylated signaling molecules. STAT3 DNA-binding capacity was studied with a colorimetric ELISA-based assay. Cell viability and invasion capability were assessed by MTS and Matrigel assays. Our results demonstrate that LIF-induced phosphorylation of STAT3 (tyr705 and ser727) is significantly increased after blocking ERK1/2. STAT3 DNA-binding capacity and cell invasiveness are enhanced after LIF stimulation and ERK1/2 blockage. In contrast, proliferation is enhanced by LIF but reduced after ERK1/2 inhibition. The findings herein show that blocking ERK1/2 increases LIF-induced STAT3 phosphorylation and STAT3 DNA-binding capacity by an intranuclear crosstalk, which leads to enhanced invasiveness and reduced proliferation.

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polA6, a mutation affecting the DNA binding capacity of DNA polymerase I

Nucleic Acids Research ◽

10.1093/nar/3.11.2971 ◽

1976 ◽

Vol 3 (11) ◽

pp. 2971-2984 ◽

Cited By ~ 20

Author(s):

W. S. Kelly ◽

N. D. F. Grindley

Keyword(s):

Dna Binding ◽

Dna Polymerase ◽

Binding Capacity ◽

Dna Polymerase I ◽

Polymerase I

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Mitosis-specific phosphorylation of the nuclear oncoproteins Myc and Myb.

The Journal of Cell Biology ◽

10.1083/jcb.118.4.775 ◽

1992 ◽

Vol 118 (4) ◽

pp. 775-784 ◽

Cited By ~ 57

Author(s):

B Lüscher ◽

R N Eisenman

Keyword(s):

Transcriptional Regulation ◽

Dna Binding ◽

Nuclear Dna ◽

Binding Capacity ◽

Dna Binding Proteins ◽

Response Element ◽

Double Stranded Dna ◽

M Phase ◽

Myb Proteins

The c-myc and c-myb proto-oncogenes encode phosphorylated nuclear DNA binding proteins that are likely to be involved in transcriptional regulation. Here we demonstrate that both Myc and Myb proteins are hyperphosphorylated during mitosis. In the case of Myb, hyperphosphorylation is accompanied by the appearance of three M phase-specific tryptic phosphopeptides. At least one of these phosphopeptides corresponds to a phosphopeptide generated after phosphorylation of Myb in vitro by p34cdc2 kinase. By contrast, the mitotic hyperphosphorylation of Myc does not correlate with the appearance of unique phosphopeptides, suggesting that M phase and interphase sites may be clustered within the same peptides. In addition Myc does not appear to be a target for p34cdc2 phosphorylation. The hyperphosphorylated forms of Myc and Myb from mitotic cells are functionally distinct from the corresponding interphase proteins in that the former have reduced ability to bind nonspecificially to double-stranded DNA cellulose. Furthermore, mitotic Myb binds poorly to oligodeoxynucleotides containing an Myb response element. We surmise that the decreased DNA binding capacity of hyperphosphorylated Myb and Myc during M phase may function to release these proteins from chromatin during chromosome condensation.

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The single-stranded DNA binding protein of Escherichia coli: physicochemical properties and biological functions

Protein-Nucleic Acid Interaction ◽

10.1007/978-1-349-09871-2_4 ◽

1989 ◽

pp. 61-86 ◽

Cited By ~ 6

Author(s):

Joachim Greipel ◽

Claus Urbanke ◽

Günter Maass

Keyword(s):

Escherichia Coli ◽

Dna Binding ◽

Physicochemical Properties ◽

Binding Protein ◽

Dna Binding Protein ◽

Biological Functions ◽

Single Stranded Dna

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Oncostatin M and leukaemia inhibitory factor trigger signal transducer and activator of transcription 3 and extracellular signal-regulated kinase 1/2 pathways but result in heterogeneous cellular responses in trophoblast cells

Reproduction Fertility and Development ◽

10.1071/rd14121 ◽

2016 ◽

Vol 28 (5) ◽

pp. 608 ◽

Cited By ~ 6

Author(s):

Wittaya Chaiwangyen ◽

Stephanie Ospina-Prieto ◽

Diana M. Morales-Prieto ◽

Francisco Lazaro Pereira de Sousa ◽

Jana Pastuschek ◽

...

Keyword(s):

Dna Binding ◽

Cell Lines ◽

Binding Capacity ◽

Binding Activity ◽

Oncostatin M ◽

Inhibitory Factor ◽

Therapeutic Interventions ◽

Leukaemia Inhibitory Factor ◽

Tetrazolium Salt ◽

Signal Transducer

Leukaemia inhibitory factor (LIF) and oncostatin M (OSM) are pleiotropic cytokines present at the implantation site that are important for the normal development of human pregnancy. These cytokines share the cell membrane receptor subunit gp130, resulting in similar functions. The aim of this study was to compare the response to LIF and OSM in several trophoblast models with particular regard to intracellular mechanisms and invasion. Four trophoblast cell lines with different characteristics were used: HTR-8/SVneo, JEG-3, ACH-3P and AC1-M59 cells. Cells were incubated with LIF, OSM (both at 10 ng mL–1) and the signal transducer and activator of transcription (STAT) 3 inhibitor S3I-201 (200 µM). Expression and phosphorylation of STAT3 (tyr705) and extracellular regulated kinase (ERK) 1/2 (thr202/204) and the STAT3 DNA-binding capacity were analysed by Western blotting and DNA-binding assays, respectively. Cell viability and invasiveness were assessed by the methylthiazole tetrazolium salt (MTS) and Matrigel assays. Enzymatic activity of matrix metalloproteinase (MMP)-2 and MMP-9 was investigated by zymography. OSM and LIF triggered phosphorylation of STAT3 and ERK1/2, followed by a significant increase in STAT3 DNA-binding activity in all tested cell lines. Stimulation with LIF but not OSM significantly enhanced invasion of ACH-3P and JEG-3 cells, but not HTR-8/SVneo or AC1-M59 cells. Similarly, STAT3 inhibition significantly decreased the invasiveness of only ACH-3P and JEG-3 cells concomitant with decreases in secreted MMP-2 and MMP-9. OSM shares with LIF the capacity to activate ERK1/2 and STAT3 pathways in all cell lines tested, but their resulting effects are dependent on cell type. This suggests that LIF and OSM may partially substitute for each other in case of deficiencies or therapeutic interventions.

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Enhanced DNA binding capacity on up‐regulated epidermal wild‐type p53 in vitiligo by H 2 O 2 ‐mediated oxidation: a possible repair mechanism for DNA damage

The FASEB Journal ◽

10.1096/fj.09-132621 ◽

2009 ◽

Vol 23 (11) ◽

pp. 3790-3807 ◽

Cited By ~ 50

Author(s):

Mohamed M. A. E. L. Salem ◽

Mohammad Shalbaf ◽

Nicholas C. J. Gibbons ◽

Bhaven Chavan ◽

J. M. Thornton ◽

...

Keyword(s):

Dna Damage ◽

Dna Binding ◽

Binding Capacity ◽

Wild Type ◽

Repair Mechanism ◽

Wild Type P53 ◽

Mediated Oxidation

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A comparative study on different physicochemical properties of fresh and frozen lamb meat

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v15i2.35077 ◽

2017 ◽

Vol 15 (2) ◽

Cited By ~ 1

Author(s):

AKM Sarwar Inam ◽

R Ahmmed ◽

MA Haque

Keyword(s):

Physicochemical Properties ◽

Texture Analysis ◽

Physicochemical Parameters ◽

Binding Capacity ◽

Color Measurement ◽

Fresh Sample ◽

Water Binding ◽

Moisture Contents ◽

Lamb Meat ◽

Significant Difference

This study described the comparison of different physicochemical parameters between fresh and frozen lamb meat. The pH measurement for fresh and frozen lamb meat did not show a significant difference. CIE L*a*b* (Commission Internationale de l'éclairage) color measurement technique was used and ∆E (distance between 2 colors) was found 5.32. On shrinkage measurement, there were significant differences (p<0.05) between the fresh and frozen meat. Frozen lamb sample showed 26.99% shrinkage compared to the fresh lamb which showed 18.09% shrinkage. The thawing loss did not show any significant difference. For texture analysis force and work were evaluated together for both fresh and frozen samples through Warner Bratzler texture analysis. The values did not show any significant difference. The absolute values of force and work were significantly different (p<0.05). Water binding capacity of the frozen and fresh sample were 56.57% and 59.27%, respectively. The moisture contents of fresh and frozen sample were 73.64% and 72.85%, respectively. Fat contents of fresh and frozen sample were 5.08% and 6.09% respectively. The study concludes that while comparing fresh and frozen lamb, only shrinkage and texture analysis showed significant difference whereas other physicochemical properties showed minor differences.J. Bangladesh Agril. Univ. 15(2): 281-288, December 2017

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Cyclin D1/cdk4 can interact with E2F4/DP1 and disrupts its DNA-binding capacity

Journal of Cellular Physiology ◽

10.1002/jcp.21243 ◽

2007 ◽

Vol 214 (3) ◽

pp. 568-581 ◽

Cited By ~ 10

Author(s):

Anthony Scimè ◽

Lili Li ◽

Gianni Ciavarra ◽

Peter Whyte

Keyword(s):

Dna Binding ◽

Cyclin D1 ◽

Binding Capacity

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