Subacute Oral Toxicity Assesment of Ethanol Extract ofMariposa christia vespertilionisLeaves in Male Sprague Dawley Rats

This toxicological assessment evaluated the safety of a hydroethanolic extract prepared from Caralluma fimbriata (CFE), a dietary supplement marketed worldwide as an appetite suppressant. Studies included 2 in vitro genotoxicity assays, a repeated dose oral toxicity study, and a developmental study in rats. No evidence of in vitro mutagenicity or clastogenicity surfaced in the in vitro studies at concentrations up to 5000 μg of extract/plate (Ames test) or 5000 μg of extract/mL (chromosomal aberration test). No deaths or treatment-related toxicity were seen in the 6-month chronic oral toxicity study in Sprague-Dawley rats conducted at 3 doses (100, 300, and 1000 mg/kg body weight (bw)/d). The no observed effect level for CFE in this study was considered to be 1000 mg/kg bw/d. A prenatal developmental toxicity study conducted at 3 doses (250, 500, and 1000 mg/kg bw/d) in female Sprague-Dawley rats resulted in no treatment-related external, visceral, or skeletal fetal abnormalities, and no treatment-related maternal or pregnancy alterations were seen at and up to the maximum dose tested. CFE was not associated with any toxicity or adverse events.

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ANTIOXIDANT AND CHEMOTHERAPEUTIC POTENTIAL OF CURCUMA AMADA RHIZOME EXTRACT ON BENZO(A)PYRENE INDUCED CERVICAL CARCINOMA IN SPRAGUE DAWLEY RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16106 ◽

2017 ◽

Vol 10 (3) ◽

pp. 235

Author(s):

Vasundra Devi Devaraj ◽

Suja Samiappan

Keyword(s):

Cervical Carcinoma ◽

Adenosine Triphosphatase ◽

Ethanol Extract ◽

Tumor Burden ◽

Ca 125 ◽

Tissue Homogenate ◽

Sprague Dawley ◽

Curcuma Amada ◽

Sprague Dawley Rats ◽

Oral Group

ABSTRACTObjective: To evaluate the antioxidant and chemotherapeutic potential of Curcuma amada Rhizome extract on benzo(a)pyrene (BaP) induced cervicalcarcinoma in Sprague Dawley rats.Methods: A total of 30 female Sprague Dawley rats were selected to establish cervical cancer model and then divided into 5 groups at random withsix mice in each group. Group 1 control, Group 2 BaP (oral), Group 3 BaP for 8 weeks and post-treated with cisplatin (intravenous administration),Group 4 BaP for 8 weeks and post-treated with 250 mg of ethanol extract of C. amada (oral), Group 5 BaP for 8 weeks and post-treated with 500 mgof ethanol extract of C. amada (oral). 4 weeks after the treatment, the animals were sacrificed, serum separated, and cervical tissues were dissected.Antioxidants and the markers carcinoembryonic antigen (CEA), cancer antigens (CAs) 125, gamma glutamyltransferase (GTT) were assayed in serumand the tissue was used for analyzing tumor burden and sectioned for histopathological assays.10% tissue homogenate was estimated for antioxidantsand membrane-bound enzymes.Results: BaP treated group showed significant (p<0.001) incidence of tumor burden, decreased activities of antioxidants, elevated lipid peroxidation,Na+/K+ adenosine triphosphatase (Na+K+ATPase), Calcium adenosine triphosphatase (Ca2+ATPase), Magnesium adenosine triphosphatase (Mg2+ ATPase),CEA, CA 125, GTT. Treatment with C. amada rhizome extract and standard drug cisplatin reverted the antioxidants, serum markers and tissue enzymes.Conclusion: From the results, it can be concluded that C. amada Rhizome extract ameliorated BaP induced oxidative stress in the cervicalcarcinogenicity of rats.Keywords: Curcuma amada, In vivo antioxidant, Chemotherapy, Benzo(a)pyrene, Cervical carcinoma, Tumor markers.

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One year oral toxicity of D-004, a lipid extract from Roystonea regia fruits, in Sprague–Dawley rats

Toxicology Letters ◽

10.1016/j.toxlet.2010.03.894 ◽

2010 ◽

Vol 196 ◽

pp. S269-S270

Author(s):

R. Gámez ◽

A. Gutiérrez ◽

D.F. Arencibia ◽

B. Pardo ◽

M. Noa ◽

...

Keyword(s):

Lipid Extract ◽

Sprague Dawley ◽

Oral Toxicity ◽

Sprague Dawley Rats ◽

One Year

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TRANSFERSOME GEL FORMULATION OF AN ETHANOL EXTRACT OF APPLES (MALUS DOMESTICA MILL) CONTAINING ANTIOXIDANTS AND IN VITRO PENETRATION TESTING USING FRANZ DIFFUSION CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.19_24 ◽

2017 ◽

Vol 9 ◽

pp. 32 ◽

Cited By ~ 1

Author(s):

Nurarita Fadila Zesiorani ◽

Effionora Anwar

Keyword(s):

Ethanol Extract ◽

Sprague Dawley ◽

Diffusion Cells ◽

Sprague Dawley Rats ◽

Apple Peel ◽

Franz Diffusion Cells ◽

Drug Efficiency ◽

And Control ◽

Peel Extract

Objective: This study aims to formulate and characterize a transfersome apple peel extract, formulate it into a gel, and compare it with a control gelmade without transfersome.Methods: Both gels were evaluated, stability tested, and penetration tested using Franz diffusion cells on the skin of female Sprague-Dawley rats. Thetransfersome preparations were formulated with different concentrations of the active substance, quercetin: 0.5% (F1); 0.7% (F2), and 1.0% (F3).Results: Based on the characterization results, F1 was selected as the optimum gel formulation because it had spherical morphology, a Dmean volume of106.44±2.70 nm, a polydispersity index of 0.078±0.01, a zeta potential of −49.96±2.05 mV, and a drug efficiency entrapment percentage of 78.78±0.46%.The cumulative amount of quercetin that was penetrated with the transfersome gel was 1514.41±26.31 μg/cm2, whereas the penetration with thecontrol gel extract was 1133.62±18.96 μg/cm2. The cumulative percentages of the penetrated gel transfersome and gel extract were 78.40±1.89%and 49.89±0.88%, respectively. The fluxes of transfersome gel and control gel extract were 52.33±0.11 μg/cm²/hrs and 40.89±0.68 μg/cm²/hrs,respectively.Conclusions: Based on these results, it can be concluded that the gel with transfersome exhibited better penetration than the gel extract alone.

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Acute and sub-acute oral toxicity Lagerstroemia speciosa in Sprague-Dawley rats

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.11.005 ◽

2021 ◽

Author(s):

Saad Alkahtani ◽

Md Saquib Hasnain ◽

Hamzah Algamdy ◽

Nada H. Aljarba ◽

Abdullah AlKahtane

Keyword(s):

Acute Oral Toxicity ◽

Sprague Dawley ◽

Oral Toxicity ◽

Lagerstroemia Speciosa ◽

Sprague Dawley Rats

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