scholarly journals ANTIOXIDANT AND CHEMOTHERAPEUTIC POTENTIAL OF CURCUMA AMADA RHIZOME EXTRACT ON BENZO(A)PYRENE INDUCED CERVICAL CARCINOMA IN SPRAGUE DAWLEY RATS

2017 ◽  
Vol 10 (3) ◽  
pp. 235
Author(s):  
Vasundra Devi Devaraj ◽  
Suja Samiappan
Keyword(s):  
Cervical Carcinoma ◽  
Adenosine Triphosphatase ◽  
Ethanol Extract ◽  
Tumor Burden ◽  
Ca 125 ◽  
Tissue Homogenate ◽  
Sprague Dawley ◽  
Curcuma Amada ◽  
Sprague Dawley Rats ◽  
Oral Group

ABSTRACTObjective: To evaluate the antioxidant and chemotherapeutic potential of Curcuma amada Rhizome extract on benzo(a)pyrene (BaP) induced cervicalcarcinoma in Sprague Dawley rats.Methods: A total of 30 female Sprague Dawley rats were selected to establish cervical cancer model and then divided into 5 groups at random withsix mice in each group. Group 1 control, Group 2 BaP (oral), Group 3 BaP for 8 weeks and post-treated with cisplatin (intravenous administration),Group 4 BaP for 8 weeks and post-treated with 250 mg of ethanol extract of C. amada (oral), Group 5 BaP for 8 weeks and post-treated with 500 mgof ethanol extract of C. amada (oral). 4 weeks after the treatment, the animals were sacrificed, serum separated, and cervical tissues were dissected.Antioxidants and the markers carcinoembryonic antigen (CEA), cancer antigens (CAs) 125, gamma glutamyltransferase (GTT) were assayed in serumand the tissue was used for analyzing tumor burden and sectioned for histopathological assays.10% tissue homogenate was estimated for antioxidantsand membrane-bound enzymes.Results: BaP treated group showed significant (p<0.001) incidence of tumor burden, decreased activities of antioxidants, elevated lipid peroxidation,Na+/K+ adenosine triphosphatase (Na+K+ATPase), Calcium adenosine triphosphatase (Ca2+ATPase), Magnesium adenosine triphosphatase (Mg2+ ATPase),CEA, CA 125, GTT. Treatment with C. amada rhizome extract and standard drug cisplatin reverted the antioxidants, serum markers and tissue enzymes.Conclusion: From the results, it can be concluded that C. amada Rhizome extract ameliorated BaP induced oxidative stress in the cervicalcarcinogenicity of rats.Keywords: Curcuma amada, In vivo antioxidant, Chemotherapy, Benzo(a)pyrene, Cervical carcinoma, Tumor markers.

scholarly journals TRANSFERSOME GEL FORMULATION OF AN ETHANOL EXTRACT OF APPLES (MALUS DOMESTICA MILL) CONTAINING ANTIOXIDANTS AND IN VITRO PENETRATION TESTING USING FRANZ DIFFUSION CELLS

2017 ◽  
Vol 9 ◽  
pp. 32 ◽  
Author(s):  
Nurarita Fadila Zesiorani ◽  
Effionora Anwar
Keyword(s):  
Ethanol Extract ◽  
Sprague Dawley ◽  
Diffusion Cells ◽  
Sprague Dawley Rats ◽  
Apple Peel ◽  
Franz Diffusion Cells ◽  
Drug Efficiency ◽  
And Control ◽  
Peel Extract

Objective: This study aims to formulate and characterize a transfersome apple peel extract, formulate it into a gel, and compare it with a control gelmade without transfersome.Methods: Both gels were evaluated, stability tested, and penetration tested using Franz diffusion cells on the skin of female Sprague-Dawley rats. Thetransfersome preparations were formulated with different concentrations of the active substance, quercetin: 0.5% (F1); 0.7% (F2), and 1.0% (F3).Results: Based on the characterization results, F1 was selected as the optimum gel formulation because it had spherical morphology, a Dmean volume of106.44±2.70 nm, a polydispersity index of 0.078±0.01, a zeta potential of −49.96±2.05 mV, and a drug efficiency entrapment percentage of 78.78±0.46%.The cumulative amount of quercetin that was penetrated with the transfersome gel was 1514.41±26.31 μg/cm2, whereas the penetration with thecontrol gel extract was 1133.62±18.96 μg/cm2. The cumulative percentages of the penetrated gel transfersome and gel extract were 78.40±1.89%and 49.89±0.88%, respectively. The fluxes of transfersome gel and control gel extract were 52.33±0.11 μg/cm²/hrs and 40.89±0.68 μg/cm²/hrs,respectively.Conclusions: Based on these results, it can be concluded that the gel with transfersome exhibited better penetration than the gel extract alone.

scholarly journals Phytochemical analysis and hepatoprotective activity of Raphanus sativus var. sativus in Sprague-Dawley rats

2020 ◽  
Vol 19 (8) ◽  
pp. 1745-1752
Author(s):  
Heshu Sulaiman Rahman ◽  
Kashan Alaalddin Bayz ◽  
Ridha Hassan Hussein ◽  
Azad Ismael Abdalla ◽  
Hemn Hassan Othman ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Phytochemical Analysis ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Hepatotoxic Effect ◽  
Hepatic Histopathology ◽  
Phenolic And Flavonoid

Purpose: To determine the phenolic and flavonoid contents of R. sativus rhizome ethanol extract and the hepatoprotective effect of the extract in rats. Methods: Folin–Ciocalteau and aluminum chloride colorimetric tests were used to determine the contents of phenols and flavonoids in the R. sativus extract. Male Sprague-Dawley rats induced with CCl4 to develop hepatotoxicity were treated orally with R. sativus extract for 4 weeks. The  antioxidant and anti-inflammatory effects of the extract on the liver were determined by evaluating the concentration of oxidative analytes, serum liver enzymes and lipids, and hepatic histopathology and cytochrome P450 2E1 expression. Results: R. sativus extract significantly (p < 0.05) reduced the hepatotoxic effect of CCl4 via its antioxidant activities and protection of liver tissues from oxidative damage. Conclusion: The hepatoprotective effects of R. sativus rhizome ethanol extract are attributed to its highphenolic and flavonoid contents. Keywords: R. savitus rhizome, Phenols, Flavonoid contents, antioxidant, Hepatoprotective

scholarly journals Gynura procumbens Standardised Extract Reduces Cholesterol Levels and Modulates Oxidative Status in Postmenopausal Rats Fed with Cholesterol Diet Enriched with Repeatedly Heated Palm Oil

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Khuzaidatul Azidah Ahmad Nazri ◽  
Norsyahida Mohd Fauzi ◽  
Fhataheya Buang ◽  
Qodriyah Haji Mohd Saad ◽  
Khairana Husain ◽  
...  
Keyword(s):  
Palm Oil ◽  
Cell Damage ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Ethanol Extract ◽  
Oxidative Status ◽  
Cholesterol Diet ◽  
Sprague Dawley ◽  
Cholesterol Levels ◽  
Sprague Dawley Rats

Gynura procumbens (Lour.) Merr. (GP) has been reported in previous studies to possess antihyperlipidaemic, antioxidative, and cardioprotective properties. This study was aimed to determine the effect of standardised 80% ethanol extract of GP on lipid profiles and oxidative status of hypercholesterolemic rats. Postmenopausal (PM) Sprague-Dawley rats were ovariectomised and fed with 2% cholesterol diet fortified with five times heated palm oil to develop hyperlipidaemia status. Two doses of the extract (250 and 500 mg/kg) and atorvastatin (10 mg/kg) were administered once daily via oral gavage for 24 weeks. Systolic blood pressure (SBP) was increased during the first month in the postmenopausal group and decreased with GP supplementation. Lipid droplets accumulation was shown at the tunica media (TM) area of the aorta in the postmenopausal group and reduced with GP supplementation. Total cholesterol (TC), total triglycerides (TG), low-density lipoprotein (LDL), and malondialdehyde (MDA) levels increased (p<0.05) at 3 and 6 months in the postmenopausal group and were reduced with GP supplementation. GP also increased high-density lipoprotein (HDL) level in the postmenopausal group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were reduced in the postmenopausal group compared to control in the sham group but increased (p<0.05) with GP supplementation. The results showed that the higher dose of GP (500 mg/kg) gave better effect. GP has the ability to reduce oxidative stress and prevent membrane cell damage through antioxidant enzyme activity modification and lipid profile changes in postmenopausal rats related to atherosclerosis.

scholarly journals Subacute Oral Toxicity Assesment of Ethanol Extract ofMariposa christia vespertilionisLeaves in Male Sprague Dawley Rats

2018 ◽  
Vol 34 (2) ◽  
pp. 85-95 ◽  
Author(s):  
Syahirah Ahmad Sayuti Nurul ◽  
Hamzah Hazilawati ◽  
Rosly Shaari Mohd ◽  
Farhan Hanif Reduan Mohd ◽  
Mohamed Mustapha Noordin ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Sprague Dawley Rats

scholarly journals Uric acid and glucose level in high fructose high cholesterol induced Sprague-Dawley rats after therapy with Acalypha indica Linn. ethanol extract

2019 ◽  
Author(s):  
Desak Gede Budi Krisnamurti ◽  
Fira Alyssa Gabriella Sinuraya ◽  
Tamara Ey Firsty ◽  
Rani Wardani Hakim ◽  
Erni H. Purwaningsih
Keyword(s):  
Uric Acid ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Sprague Dawley ◽  
High Cholesterol ◽  
Sprague Dawley Rats ◽  
High Fructose ◽  
Acalypha Indica

scholarly journals ROSELLE CALYX ETHANOL EXTRACT STIMULATES ORAL MUCOSAL WOUND HEALING

2018 ◽  
Vol 9 ◽  
pp. 36
Author(s):  
Sarwono At ◽  
Suniarti Df
Keyword(s):  
Wound Healing ◽  
Ethanol Extract ◽  
Lymphocytic Infiltration ◽  
Punch Biopsy ◽  
Sprague Dawley ◽  
Standard Temperature ◽  
Sprague Dawley Rats ◽  
Similar Formation ◽  
Wounded Area ◽  
Mucosal Wound

Objective: This study aim to analyze the effect of the roselle calyx ethanol extract on oral mucosal wound healing.Methods: Male Sprague Dawley rats (3 month, 150 g) were housed under standard temperature conditions (20–22°C). Punch biopsy wounds were inflicted on the inferior labial mucosal membrane of rats. All treatments (normal saline; 0.1% triamcinolone acetonide; and 3.75%, 7.5%, and 15% roselle calyx ethanol extract) were applied 2 times daily. Wounded areas were observed after 8 days of treatment. The specimen of the wounded area was prepared and microscopic slides were analyzed under a light microscope.Results: The histological features of the groups treated with TA and 7.5% roselle calyx ethanol extract showed a similar formation of the epidermis layer and no lymphocytic infiltration of the lamina propria.Conclusion: The application of 7.5% roselle calyx ethanol extract could stimulate oral mucosal wound healing.

scholarly journals Ethanol extract of Psoralea corylifolia L. and its main constituent, bakuchiol, reduce bone loss in ovariectomised Sprague–Dawley rats

2008 ◽  
Vol 101 (7) ◽  
pp. 1031-1039 ◽  
Author(s):  
Sun-Hye Lim ◽  
Tae-Youl Ha ◽  
Sung-Ran Kim ◽  
Jiyun Ahn ◽  
Hyun Jin Park ◽  
...  
Keyword(s):  
Bone Loss ◽  
Binding Affinity ◽  
Ethanol Extract ◽  
Treated Group ◽  
Vehicle Group ◽  
Psoralea Corylifolia ◽  
Sprague Dawley ◽  
Mineral Density ◽  
Sprague Dawley Rats

The aim of the present study was to investigate whether ethanol extracts of Psoralea corylifolia L. (PCE) and its active component protect against bone loss in ovariectomised rats. We screened oestrogenic activities of the main extract fractions using in vitro assays and identified bakuchiol as the most active oestrogenic component by HPLC and LC/MS, and then demonstrated that bakuchiol had strong binding affinity for oestrogen receptor (ER) α. Seventy female Sprague–Dawley rats were assigned to either a sham-operated group (n 10) or an ovariectomised group (n 60). The ovariectomised group was subdivided into six groups, each containing ten rats: vehicle group, two bakuchiol-treated groups (dose of 15 mg/kg per d or 30 mg/kg per d; ten rats for each group), two PCE-supplemented groups (0·25 % or 0·5 % extracts of diets; ten rats for each group) and a 17β-oestradiol (E2)-treated group (20 μg/kg per d). We recorded weight and feed intake every week, and killed all animals after 6 weeks. Blood was collected, and the uterus, kidneys and livers were removed. Bakuchiol has a three-fold higher binding affinity for ERα than for ERβ. Bakuchiol and PCE treatments had no uterotrophic activity even though they demonstrated oestrogenic activity in the in vitro assays. Bakuchiol and PCE treatments reduced postmenopausal bone loss by increasing alkaline phosphatase, Ca concentrations, serum E2 concentration and bone mineral density, and by decreasing the inorganic P level. The present study indicated that bakuchiol and PCE treatments could protect against bone loss.

scholarly journals Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats

Molecules ◽  
2012 ◽  
Vol 17 (3) ◽  
pp. 2796-2811 ◽  
Author(s):  
Ibrahim Abdel Aziz Ibrahim ◽  
Suhailah Wasmn Qader ◽  
Mahmood Ameen Abdulla ◽  
Amal R. Nimir ◽  
Siddig Ibrahim Abdelwahab ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Sprague Dawley ◽  
Sprague Dawley Rats
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