scholarly journals In Vitro Investigation of Surface Roughness and Bacterial Adhesion on Different Dental Porcelains Applying Different Vital Whitening Agents

2021 ◽  
Vol 1 (1) ◽  
pp. 21-35
Author(s):  
Nesrin Şahin ◽  
Sevil Meral Sahmali ◽  
Pelin Özmen
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
In Vitro Investigation

scholarly journals In vitro Evaluation of Structural Factors Favouring Bacterial Adhesion on Orthodontic Adhesive Resins

2021 ◽  
Author(s):  
Roberta Condò ◽  
Gianluca Mampieri ◽  
Guido Pasquantonio ◽  
Aldo Giancotti ◽  
Paola Pirelli ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Structural Factors ◽  
Adhesive Material ◽  
Internal Structures ◽  
Structural Nature ◽  
Beam Technique

Bacterial adhesion to the surface of the adhesive material is an important step in the formation of plaque and enamel demineralization. In order to correlate the material composition to the specific surface roughness of the resin and to the probable more favourable adhesion of bacteria, scanning electron microscopy, combined with focus ion bean micromachining, together with stylus profilometry analysis have been in vitro performed to reveal the structural nature of three orthodontic adhesive resins used for bracket bonding and, above all, to understand how compositional factors can influence specific pivotal properties such as material’s surface roughness and robustness. In particular, we speculated about the morphological features that determine an increase in the bacterial adhesion and we proposed focused ion beam technique as a valuable tool to compare the internal structures of the polymers and to determine the peculiar mechanical properties of the examined adhesive resins.

In Vitro Impact of a Glaze/ Composite Resin Sealant on the Surface Roughness and Bacterial Adhesion of One Microhybrid and Three Nanofilled Composite Resins

2013 ◽  
Vol 3 (2) ◽  
pp. 130-144
Author(s):  
Salah Hasab Mahmoud ◽  
Naglaa Rezk El-Kholany ◽  
Mohammed EL-Awady Grawish ◽  
Salwa Abd El-Raof El- Negoly
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Composite Resin ◽  
Composite Resins ◽  
Resin Sealant

The Effect of the Surface Roughness of Porcelain on the Adhesion of Oral Streptococcus mutans

2009 ◽  
Vol 10 (6) ◽  
pp. 17-24 ◽  
Author(s):  
Maan Ibrahim Al-Marzok ◽  
Haitham J. Al-Azzawi
Keyword(s):  
Surface Roughness ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Selective Medium ◽  
Surface Finishing ◽  
Bacterial Suspension ◽  
Sterile Saline ◽  
Glazed Surface ◽  
Glazed Porcelain

Abstract Aim Dental plaque has a harmful influence on periodontal tissue. When a porcelain restoration is fabricated and refinishing of the glazed surface is inevitable, the increase in surface roughness facilitates the adhesion of plaque and its components. The aim of this in vitro study was to evaluate the effect of surface roughness of glazed or polished porcelain on the adhesion of oral Streptococcus mutans. Methods and Materials A total of 80 metalceramic specimens were prepared in the form of disks from two porcelain materials and divided into four groups according to the method of surface finishing. Surface roughness values (Ra-μm) for all specimens were recorded using a profilometer. S. mutans bacteria were isolated from saliva and all specimens were inoculated in test tubes containing a bacterial suspension allowing adhesion of the microorganisms to the specimens to occur. After incubation for 24 hours at 37°C, the specimens were transferred to a sterile saline solution and an inoculum of 0.1 ml from each selected dilution was spread on the selective medium, mitis salivarius bacitracin agar (MSB). Bacterial counts, expressed in colony forming unit (CFU) taking into consideration the dilution factor, were recorded. Results There was significant correlation (p<0.05) between surface roughness values (Ra-μm) and the amount of bacterial adhesion (CFU×103). The glazed surface was the smoothest and exhibited the least amount of bacterial adhesion. Conclusions A positive correlation between surface roughness and the amount of S. mutans adhesion was observed. The glazed porcelain surface was considered more biocompatible than other methods of porcelain surface finishing. Clinical Significance Chairside adjustments of the cervical contour or occlusal surface of porcelain restorations are sometimes necessary before or after cementation. Ideally, an uncemented restoration should be returned to the laboratory for reglazing after all adjustments have been completed. It is important to evaluate various polishing procedures used for these adjusted surfaces to achieve a finished surface that as closely as possible approximates the quality of glazed porcelain. Citation Al-Marzok MI, Al-Azzawi HJ. The Effect of the Surface Roughness of Porcelain on the Adhesion of Oral Streptococcus mutans. J Contemp Dent Pract [Internet]. 2009 Nov; 10(6):017-024. Available from: http://www.thejcdp. com/journal/view/volume10-issue6-al-marzok.

Effect of pH Cycling Followed by Simulated Toothbrushing on the Surface Roughness and Bacterial Adhesion of Bulk-fill Composite Resins

2020 ◽  
Vol 45 (2) ◽  
pp. 209-218 ◽  
Author(s):  
DC Somacal ◽  
FB Manfroi ◽  
MSG Monteiro ◽  
SD Oliveira ◽  
HR Bittencourt ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Composite Resin ◽  
Composite Resins ◽  
Sem Images ◽  
Effect Of Ph ◽  
Group 4 ◽  
Ph Cycling ◽  
Group 2

SUMMARY The aim was to evaluate, in vitro, quantitatively and qualitatively, the effect of pH cycling and simulated toothbrushing on surface roughness (Ra) and bacterial adhesion (Cn) of bulk-fill composite resins. Thirty specimens of each composite resin, 5 mm wide and 4 mm high, were obtained: group 1 (control): Filtek Z250 (Z250); group 2: Filtek Bulk-Fill (FTK); group 3: Tetric N-Ceram Bulk-Fill (TTC); and group 4: Aura Bulk-Fill (AUR). After 24 hours, the specimens were polished and then alternated with demineralization/remineralization solutions for 15 cycles of 24 hours each at 37°C. Then the specimens were submitted to simulated toothbrushing. The Ra and Cn measurements were quantitatively analyzed in three stages: after polishing (Ra0 and Cn0), after pH cycling (Ra1 and Cn1), and after simulated toothbrushing (Ra2 and Cn2). The Ra values were submitted to two-way analysis of variance, followed by the Tukey test (α=0.05). The Kruskal-Wallis test, followed by multiple comparisons, was applied for Cn analysis. Surface topography and bacterial adhesion were observed by scanning electron microscopy (SEM). Z250, FTK, and TTC showed no significant change in Ra regardless of the treatment performed; AUR obtained increased Ra at Ra2 (p&lt;0.05). FTK differed from the others at Cn0 and Cn1 (p&lt;0.05). At Cn2, there was no difference among the composite resins. SEM images showed the exposure of fillers and microcavities at Ra1 and Ra2. There was greater bacterial adhesion at Cn1 for Z250 and FTK. It was concluded that the pH cycling caused surface degradation of all composite resins, which was potentiated by simulated toothbrushing. However, only AUR presented an increased Ra. Bacterial adhesion occurred on all composite resins after pH cycling; however, after simulated toothbrushing, adhesion of dispersed bacteria was similar for all the composite resin groups.

scholarly journals In vitro and in vivo biofilm adhesion to esthetic coated arch wires and its correlation with surface roughness

2015 ◽  
Vol 86 (2) ◽  
pp. 285-291 ◽  
Author(s):  
Mahasen Taha ◽  
Abeer El-Fallal ◽  
Heba Degla
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Test Analysis ◽  
Positive Correlation ◽  
Oral Biofilms ◽  
Orthodontic Patients ◽  
In Vitro Adhesion ◽  
Level Of Significance

ABSTRACT Objective:  To evaluate the in vitro ability of esthetic coated rectangular arch wires to retain oral biofilms and in vivo biofilm formation on these wires after 4 and 8 weeks of clinical use and to correlate the findings with the surface roughness of these wires. Materials and Methods:  Three brands of esthetic coated nickel-titanium (NiTi) arch wires were selected. Arch wires retrieved after 4 and 8 weeks of intraoral use were obtained from 30 orthodontic patients. Surface roughness (SR) was assessed with an atomic force microscope. In vitro adhesion assays were performed using Streptococcus mutans (MS), Staphylococcus aureus, and Candida albicans. The amount of bacterial adhesion was quantified using the colony-count method. Paired t-test, analysis of variance, post hoc Tukey's test, and Pearson's correlation coefficient test were used for statistical analysis at the .05 level of significance. Results:  In vitro bacterial adhesion showed significant differences between wires in terms of MS adhesion (P  =  .01). All wires showed significant increases in SR (P  =  .001 after 4 weeks and .007 after 8 weeks) and biofilm adhesion (P  =  .0001 after 4 weeks and .045 after 8 weeks) after intraoral exposure. A significant positive correlation (P  =  .001 after 4 weeks and .05 after 8 weeks) was observed between these two variables in vivo, but the correlation was not significant for in vitro bacterial adhesion. Conclusions:  SR and biofilm adhesion increased after intraoral use at all time intervals. There was a positive correlation between SR and biofilm adhesion in vivo only.

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