scholarly journals In vitro and in vivo biofilm adhesion to esthetic coated arch wires and its correlation with surface roughness

2015 ◽  
Vol 86 (2) ◽  
pp. 285-291 ◽  
Author(s):  
Mahasen Taha ◽  
Abeer El-Fallal ◽  
Heba Degla
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Test Analysis ◽  
Positive Correlation ◽  
Oral Biofilms ◽  
Orthodontic Patients ◽  
In Vitro Adhesion ◽  
Level Of Significance

ABSTRACT Objective:  To evaluate the in vitro ability of esthetic coated rectangular arch wires to retain oral biofilms and in vivo biofilm formation on these wires after 4 and 8 weeks of clinical use and to correlate the findings with the surface roughness of these wires. Materials and Methods:  Three brands of esthetic coated nickel-titanium (NiTi) arch wires were selected. Arch wires retrieved after 4 and 8 weeks of intraoral use were obtained from 30 orthodontic patients. Surface roughness (SR) was assessed with an atomic force microscope. In vitro adhesion assays were performed using Streptococcus mutans (MS), Staphylococcus aureus, and Candida albicans. The amount of bacterial adhesion was quantified using the colony-count method. Paired t-test, analysis of variance, post hoc Tukey's test, and Pearson's correlation coefficient test were used for statistical analysis at the .05 level of significance. Results:  In vitro bacterial adhesion showed significant differences between wires in terms of MS adhesion (P  =  .01). All wires showed significant increases in SR (P  =  .001 after 4 weeks and .007 after 8 weeks) and biofilm adhesion (P  =  .0001 after 4 weeks and .045 after 8 weeks) after intraoral exposure. A significant positive correlation (P  =  .001 after 4 weeks and .05 after 8 weeks) was observed between these two variables in vivo, but the correlation was not significant for in vitro bacterial adhesion. Conclusions:  SR and biofilm adhesion increased after intraoral use at all time intervals. There was a positive correlation between SR and biofilm adhesion in vivo only.

scholarly journals Biocompatibility Evaluation of Electrospun Scaffolds of Poly (L-Lactide) with Pure and Grafted Hydroxyapatite

2017 ◽  
Vol 58 (4) ◽  
Author(s):  
José Manuel Cornejo-Bravo ◽  
Luis Jesús Villarreal-Gómez ◽  
Ricardo Vera-Graziano ◽  
María Raquel Vega-Ríos ◽  
José Luis Pineda-Camacho ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Neutral Red ◽  
C2c12 Cells ◽  
Surrounding Tissue ◽  
Neutral Red Assay ◽  
Electrospun Scaffolds ◽  
Muscle Tissues ◽  
Hematoxylin Eosin

<p>The objective of this work was to evaluate the biocompatibility of scaffolds of poly(<em>L</em>-lactide) with pure and grafted hydroxyapatite, at various concentrations of reinforcement. The biocompatibility tests were carried out <em>in vivo </em>in Wistar rats by implanting the material into the subcutaneous and muscle tissues from 1 to 14 weeks and evaluating the surrounding tissue stained with hematoxylin-eosin. For <em>in vitro </em>assays, MTT and neutral red assay were used to evaluate any cytotoxicity in Mioblast Muscle C2C12 Cells (ATCC® CRL-1772™) and Bovine Coronary Artery Endothelial Cells (BCAEC); <em>Escherichia coli </em>and <em>Staphylococcus aureus </em>were used to evaluate bacterial adhesion. All variants of scaffolds provoked a mild inflammatory response, without showing necrosis. No evidence of cytotoxicity was presented in cell viability tests and good bacterial cell adhesion was visualized for all of the materials studied.</p>

scholarly journals Effects of Systemic Fluoride and in vitro Fluoride Treatment on Enamel Crystals

2006 ◽  
Vol 85 (11) ◽  
pp. 1042-1045 ◽  
Author(s):  
H. Chen ◽  
A. Czajka-Jakubowska ◽  
N.J. Spencer ◽  
J.F. Mansfield ◽  
C. Robinson ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Crystal Surface ◽  
Direct Action ◽  
Crystal Surfaces ◽  
Surface Restructuring ◽  
Fluoride Treatment ◽  
Enamel Crystal ◽  
Dose Dependent

Systemically administered fluoride at a concentration of 75 ppm increases the surface roughness of developing enamel crystals in rats, which may be significant in advancing our understanding of the biological mechanism of fluorosis. Thus, the aim of this study was to investigate whether the increased surface roughness may be a result of surface restructuring by the direct action of fluoride at the crystal surface. We examined the fluoride dose-dependent roughening of enamel crystal surfaces in vivo, in the rat, and whether this roughening could be mimicked by the in vitro treatment of rat enamel crystals with neutral pH fluoride solutions. Our results showed that enamel crystal surface roughness increased after treatment with increasing fluoride ion concentrations, whether applied in vitro or administered systemically. This suggests a mechanism, alongside others, for the increased surface roughness of crystals in fluorotic enamel.

Bacterial Adhesion on Polyelectrolyte Modified Microstructured Titanium Surfaces

2010 ◽  
Vol 1277 ◽  
Author(s):  
Argelia Almaguer-Flores ◽  
Yolloxóchilt R. Sánchez-Cruz ◽  
Jung Hwa Park ◽  
René Olivares-Navarrete ◽  
Michel Dard ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bacterial Adhesion ◽  
Statistical Significance ◽  
Fusobacterium Nucleatum ◽  
Bacterial Attachment ◽  
Chemical Surface ◽  
Campylobacter Rectus ◽  
Submicron Scale ◽  
Student’S T

Micron-scale and submicron-scale surface roughness enhance osteoblast differentiation on titanium (Ti) substrates and increases bone-to-implant contact in vivo. However, bacterial adhesion is also strongly influenced by roughness and surface chemistry. The aim of this study was to investigate if chemical surface modifications alter initial bacterial attachment. To achieve this, two polyelectrolyte layers [chitosan (Ch) and poly(L-lysine) (PLL)] were used to coat Ti surfaces with different roughness (PT [Ra<0.3μm], SLA [Ra≥3.0μm]). Bacterial attachment was evaluated using Aggregatibacter actinomycetemcomitans, Actinomyces israelii, Campylobacter rectus, Eikenella corrodens, Fusobacterium nucleatum, Parvimonas micra, Porphyromonas gingivalis, Prevotella intermedia and Streptococcus sanguinis. After 24h incubation, bacteria were detached from the samples with sonication and the counting plate technique was performed to determine the number of colony forming units (CFU's). Additionally, surfaces were observed by scanning electron microscopy to determine bacteria surface coverage. Statistical significance was determined using ANOVA followed by Bonferroni's modification of Student's t-test. The results showed that polyelectrolyte coatings did not affect surface roughness. Modified surfaces were more hydrophilic than the controls. PT surfaces covered by Chi exhibited lower CFUs than the same surface covered by PLL or the control PT (140 × 105/mL, 343 × 105/mL and 283 × 105/mL, respectively). The opposite effect was observed on the SLA surfaces, PLL coated samples shown lower CFUʼs than Chi or uncoated SLA (199 × 105/mL, 229 × 105/mL and 227 × 105/mL, respectively). The Chi layer appeared to reduce bacterial adhesion only on the smooth surfaces. In contrast, PLL coatings reduced bacterial attachment on rougher surfaces. These results suggest that chemical modification of Ti without alteration of surface roughness affects oral bacterial attachment, and could be useful to prevent peri-implantitis related diseases.

scholarly journals EphA3 targeting reduces in vitro adhesion and invasion and in vivo growth and angiogenesis of multiple myeloma cells

2017 ◽  
Vol 40 (5) ◽  
pp. 483-496 ◽  
Author(s):  
Francesco La Rocca ◽  
Irma Airoldi ◽  
Emma Di Carlo ◽  
Pina Marotta ◽  
Geppino Falco ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Myeloma Cells ◽  
In Vitro Adhesion ◽  
In Vivo Growth ◽  
Adhesion And Invasion

Protein antimicrobial barriers to bacterial adhesion: in vitro and in vivo evaluation of nisin-treated implantable materials

2002 ◽  
Vol 25 (1) ◽  
pp. 81-90 ◽  
Author(s):  
C.K Bower ◽  
J.E Parker ◽  
A.Z Higgins ◽  
M.E Oest ◽  
J.T Wilson ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
In Vivo Evaluation

scholarly journals Defining surfaces : Implant topography – a review (Preprint)

2018 ◽  
Author(s):  
Preeti Satheesh Kumar ◽  
Vyoma Venkatesh Grandhi ◽  
Vrinda Gupta
Keyword(s):  
Surface Roughness ◽  
Surface Topography ◽  
Dental Implants ◽  
Sol Gel ◽  
Implant Surface ◽  
Research Publications ◽  
Bone To Implant Contact ◽  
Titanium Dental Implants

BACKGROUND . A variety of claims are made regarding the effects of surface topography on implant osseointegration. The development of implant surfaces topography has been empirical, requiring numerous in vitro and in vivo tests. Most of these tests were not standardized, using different surfaces, cell populations or animal models. The exact role of surface chemistry and topography on the early events of the osseointegration of dental implants remain poorly understood. OBJECTIVE This review considers the major claims made concerning the effects of titanium implant surface topography on osseointegration. The osseointegration rate of titanium dental implants is related to their composition and surface roughness. The different methods used for increasing surface roughness or applying osteoconductive coatings to titanium dental implants are reviewed. Important findings of consensus are highlighted, and existing controversies are revealed. METHODS This review considers many of the research publications listed in MEDLINE and presented in biomedical research publications and textbooks. Surface treatments, such as titanium plasma-spraying, grit-blasting acid-etching,alkaline etching, anodization,polymer demixing ,sol gel conversion and their corresponding surface morphologies and properties are described. RESULTS Many in vitro evaluations are not predictive of or correlated with in vivo outcomes. In some culture models, increased surface topography positively affects pro-osteogenic cellular activities. Many studies reveal increase in bone-to-implant contact,with increased surface topography modifications on implant surfaces. CONCLUSIONS Increased implant surface topography improves the bone-to-implant contact and the mechanical properties of the enhanced interface.

scholarly journals Evaluation of enamel roughness after the removal of brackets bonded with different materials: In vivo study

2021 ◽  
Vol 32 (5) ◽  
pp. 34-40
Author(s):  
Rodrigo Vieira Caixeta ◽  
Sandrine Bittencourt Berger ◽  
Murilo Baena Lopes ◽  
Eloisa Aparecida Carlesse Paloco ◽  
Élcio Mário Faria-Júnior ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Initial Conditions ◽  
Glass Ionomer Cement ◽  
Enamel Surface ◽  
In Vivo Study ◽  
Adhesive Remnant Index ◽  
Resin Modified Glass Ionomer ◽  
Before And After ◽  
Orthodontic Patients

Abstract The aims of this in vivo study were to evaluate the effect of bonding with resin-modified glass ionomer cement (RMGIC) and to assess enamel surface roughness before and after the removal of brackets bonded with composite or RMGIC from the maxillary central incisors. Fifteen orthodontic patients were selected for the study. For each patient, the teeth were rinsed and dried, and brackets were bonded with composite (Transbond XT) and RMGIC (Vitremer Core Buildup/Restorative). At the conclusion of orthodontic treatment, their brackets were removed. Dental replicas were made of epoxy resin in initial conditions (before bonded) and after polishing with an aluminum oxide disc system. Adhesive remnant index (ARI) and surface roughness was measured on the dental replicas and data were evaluated statistically by Mann-Whitney and paired t-test, respectively. No bracket debonding occurred during patients’ treatment periods. It was verified that the ARI values of the two maxillary central incisors were similar (p = 0.665). For both bonding materials, the ARI value of 3 was predominant. After polishing, surface roughness was similar in the composite and RMGIC groups (0.245 μm and 0.248 μm, respectively; p = 0.07). In both groups, enamel surface roughness values were significantly lower after polishing compared with the initial condition (p < 0.001). RMGIC promoted efficiency in cementing brackets without fail during treatment; the choice of composite or RMGIC materials was not a factor that influenced the roughness of the enamel surface, however, polishing led to smoother surfaces than those found at the beginning of the treatment.

scholarly journals Efficacy of Light-Activated Sealant on Enamel Demineralization in Orthodontic Patients: An Atomic Force Microscope Evaluation

2011 ◽  
Vol 5 (1) ◽  
pp. 179-186 ◽  
Author(s):  
Suzi F Shinaishin ◽  
Safaa A Ghobashy ◽  
Tarek H EL-Bialy
Keyword(s):  
Surface Area ◽  
Orthodontic Treatment ◽  
Total Surface Area ◽  
Roughness Height ◽  
Fluoride Varnish ◽  
Enamel Demineralization ◽  
Atomic Force ◽  
Orthodontic Patients ◽  
Level Of Significance

Objective: The purpose of this study was to investigate the efficacy of (Pro Seal) sealant in preventing enamel decalcification in-vivo and compare its effect with fluoride varnish and unfilled sealant using atomic force microscopy. Materials and Methods: Eight orthodontic patients who were candidates for extraction of all first premolars for orthodontic treatment were recruited to this study. Thirty two premolars (upper and lower) were randomly divided into four groups (n=8) for each group, 4 maxillary and 4 mandibular); Control (no -treatment); Fluoride varnish, Unfilled sealant (Light Bond) and filled sealant (Pro-Seal). After two months the brackets were debonded and the teeth were extracted and prepared for Atomic force microscopic scanning. Each sample was scanned twice at two different scan areas 50 and 10µm at the buccal cervical third of the crown. Images were recorded with slow scan rate and resolution and the mean roughness height and total surface area were calculated for each scan area. Comparison between groups was performed using one way analysis of variance test with level of significance was set to be 0.05. Results: Pro Seal treated samples show the lowest roughness height and total surface area. Conclusion: Pro Seal was the most effective prophylaxis technique in preventing enamel demineralization during orthodontic treatment.

Functional Analyses of Patient-derived IgG Monoclonal Anticardiolipin Antibodies Using In Vivo Thrombosis and In Vivo Microcirculation Models

2000 ◽  
Vol 84 (09) ◽  
pp. 388-395 ◽  
Author(s):  
Silvia Pierangeli ◽  
Xiaowei Liu ◽  
Ricardo Espinola ◽  
Tsawei Olee ◽  
Min Zhu ◽  
...  
Keyword(s):  
Cell Function ◽  
Leukocyte Adhesion ◽  
Thrombus Formation ◽  
Anticardiolipin Antibodies ◽  
H2 Control ◽  
Thrombosis Model ◽  
Enhanced Expression ◽  
In Vitro Adhesion

SummaryAntiphospholipid antibodies (aPL) have been associated with thrombosis and pregnancy losses in patients diagnosed with antiphospholipid syndrome (APS) and enhance thrombus formation in vivo in mice, but the mechanism of thrombosis by aPL is not completely understood. It has been proposed that aPL may affect endothelial cell (EC) function and/or induce their activation, transforming their anticoagulant surface into procoagulant, thus predisposing to thrombosis. It has been proposed that aPL may affect EC cell function and/or induce their activation, transforming their anticoagulant surface into procoagulant, thus predisposing to thrombosis. This study proposes to test the hypotheses that some IgG anticardiolipins (IgG aCL) with thrombogenic properties in mice, exert their effects through activation of endothelium. We studied seven patient-derived monoclonal aCL for their thrombogenic properties in an in vivo pinch-induced thrombosis model, and their functional activities in activating EC by analyzing in vivo leukocyte adhesion to endothelium in microcirculation in venules in exposed murine cremaster muscle and in vitro adhesion molecule expression in cultured EC. The binding of the monoclonal aCL to EC was also tested. In addition to the previous identified thrombogenic IS2, four of the five new more IgG monoclonal aCL (from two patients) were found to be thrombogenic. Of these five thrombogenic aCL, three caused more in vivo leukocyte adhesion to EC in microcirculation, as compared to that induced by the H2 control human monoclonal IgG, and enhanced expression of adhesion molecules (particularly VCAM-1) on cultured EC. These data show that about 2/3 patientderived IgG monoclonal aCL are thrombogenic and suggest that some thrombogenic IgG aCL exert their effects through activating EC.

Sign in / Sign up

Export Citation Format

Share Document