scholarly journals Optimization of Subcritical Water Extraction of Polysaccharides from Inonotus Obliquus and their Antioxidant Activities

2017 ◽  
Vol 9 (3) ◽  
pp. 38 ◽  
Author(s):  
Xi Yuan ◽  
Ling Li ◽  
Hongyi Sun ◽  
Shuang Sun ◽  
Zhenya Zhang
Keyword(s):  
Subcritical Water ◽  
Antioxidant Activities ◽  
Hot Water ◽  
Water Extraction ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Inonotus Obliquus ◽  
Solid Ratio ◽  
Ft Ir

Subcritical water extraction (SWE) of Inonotus Obliquus polysaccharides (IOP) was investigated using response surface methodology (RSM) with a design by Box–Behnken design (BBD). Results showed that the optimum SWE conditions for IOP production were as follows: extraction temperature 194°C, residence time 5.36 min and liquid-solid ratio 53 mL/g, yielding 168.80 ± 0.59 mg/g of IOP, which was in close agreement with the values predicted by the mathematical model. FT-IR spectra of the polysaccharides extracted by SWE and hot water extraction (HWE) were compared as well. Moreover, in vitro antioxidant assays revealed that SWE-IOP exhibited stronger scavenging activity that HWE-IOP. This investigation suggests that polysaccharides of Inonotus Obliquus extracted by SWE could be further developed as a potential antioxidant resource for dietary supplements of functional food.

Antioxidant activities of onion (Allium cepa L.) peel extracts produced by ethanol, hot water, and subcritical water extraction

2014 ◽  
Vol 23 (2) ◽  
pp. 615-621 ◽  
Author(s):  
Kyoung Ah Lee ◽  
Kee-Tae Kim ◽  
Hyun Jung Kim ◽  
Myong-Soo Chung ◽  
Pahn-Shick Chang ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Subcritical Water ◽  
Antioxidant Activities ◽  
Hot Water ◽  
Water Extraction ◽  
Subcritical Water Extraction ◽  
Allium Cepa L

scholarly journals Nutritional Value and Biofunctionalities of Two Edible Green Seaweeds (Ulva lactuca and Caulerpa racemosa) from Indonesia by Subcritical Water Hydrolysis

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 578
Author(s):  
Ratih Pangestuti ◽  
Monjurul Haq ◽  
Puji Rahmadi ◽  
Byung-Soo Chun
Keyword(s):  
Bioactive Compounds ◽  
Subcritical Water ◽  
Antioxidant Activities ◽  
Ulva Lactuca ◽  
Hot Water ◽  
Water Extraction ◽  
Raw 264.7 Cells ◽  
Total Phenolic ◽  
Subcritical Water Extraction ◽  
Caulerpa Racemosa

Caulerpa racemosa (sea grapes) and Ulva lactuca (sea lettuces) are edible green seaweeds and good sources of bioactive compounds for future foods, nutraceuticals and cosmeceutical industries. In the present study, we determined nutritional values and investigated the recovery of bioactive compounds from C. racemosa and U. lactuca using hot water extraction (HWE) and subcritical water extraction (SWE) at different extraction temperatures (110 to 230 ºC). Besides significantly higher extraction yield, SWE processes also give higher protein, sugar, total phenolic (TPC), saponin (TSC), flavonoid contents (TFC) and antioxidant activities as compared to the conventional HWE process. When SWE process was applied, the highest TPC, TSC and TFC values were obtained from U. lactuca hydrolyzed at reaction temperature 230 ºC with the value of 39.82 ± 0.32 GAE mg/g, 13.22 ± 0.33 DE mg/g and 6.5 ± 0.47 QE mg/g, respectively. In addition, it also showed the highest antioxidant activity with values of 5.45 ± 0.11 ascorbic acid equivalents (AAE) mg/g and 8.03 ± 0.06 trolox equivalents (TE) mg/g for ABTS and total antioxidant, respectively. The highest phenolic acids in U. lactuca were gallic acid and vanillic acid. Cytotoxic assays demonstrated that C. racemosa and U. lactuca hydrolysates obtained by HWE and SWE did not show any toxic effect on RAW 264.7 cells at tested concentrations after 24 h and 48 h of treatment (p < 0.05), suggesting that both hydrolysates were safe and non-toxic for application in foods, cosmeceuticals and nutraceuticals products. In addition, the results of this study demonstrated the potential of SWE for the production of high-quality seaweed hydrolysates. Collectively, this study shows the potential of under-exploited tropical green seaweed resources as potential antioxidants in nutraceutical and cosmeceutical products.

Ultrasonic-Assisted Aqueous Two-Phase Extraction Combined with Macroporous Resin Enrichment of Lignans from Flaxseed Meal and Their Antioxidant Activities

2021 ◽  
Author(s):  
Zhizhou Zhang ◽  
Yongchao Zhang ◽  
Yichao Wu ◽  
Yongxiang Xu ◽  
Yuanyuan Jiang ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Phase System ◽  
Extraction Temperature ◽  
Macroporous Resin ◽  
Two Phase ◽  
Solid Ratio ◽  
Flaxseed Meal ◽  
Extraction And Purification ◽  
Ultrasonic Assisted

Abstract Background Secoisolariciresinol di glucoside (SDG) is a natural antioxidant generally extracted from flaxseed, which is one of the most important oil crops in China, the by-product of the flaxseed oil, i.e., flaxseed meal, still contains a lot of lignans. However, flaxseed meal is generally treated as waste, resulting in a huge waste of resources. Objective To establish an efficient and convenient method for extraction and purification of lignans from flaxseed meal. Methods First, we used response surface methodology (RSM) to optimize the extraction conditions of the ultrasonic-assisted aqueous two-phase system (UAATPS), and we obtained the purified extracts by macroporous resin purification (MRP). Second, the antioxidant ability of the extracts was studied in vitro. Results The best extraction conditions obtained were as follows: 9.0 wt% sodium hydroxide, 30.0 wt% isopropanol, extraction time 39 min, liquid-to-solid ratio of 52.0 mL/g, ultrasonic wave 560 W, and extraction temperature 40 °C. Under the optimal conditions, the purity of crude extracts (SDG-APTS-C) reached 21.5%. The desorption conditions of MRP were as follows: eluting 3 BV with ultrapure water, and then eluting with 25% ethanol at 2 BV/h to collect eluents. The purified extracts (SDG-ATPS-P) had a purity quotient of 73.9%, 52.4% higher than that of SDG-ATPS-C. Additionally, experiments conducted in this paper revealed that SDG-ATPS-C and SDG-ATPS-P could effectively remove DPPH, ABTS, and hydroxyl free radicals in vitro. Conclusions The method was validated for extracting SDG from flaxseed meal, thus achieving the reuse of flaxseed meal. Highlights This research provides some references for the application of UAATPS combined with MRP in natural products.

scholarly journals Production of Ulvan Oligosaccharides with Antioxidant and Angiotensin-Converting Enzyme-Inhibitory Activities by Microbial Enzymatic Hydrolysis

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 160
Author(s):  
Yueh-Hao Ronny Hung ◽  
Guan-Wen Chen ◽  
Chorng-Liang Pan ◽  
Hong-Ting Victor Lin
Keyword(s):  
Enzymatic Hydrolysis ◽  
Antioxidant Activities ◽  
Ace Inhibition ◽  
Aeromonas Salmonicida ◽  
Water Soluble ◽  
Hot Water ◽  
Converting Enzyme ◽  
Green Seaweed ◽  
Ft Ir

Seaweed oligosaccharides have attracted attention in food, agricultural, and medical applications recently. Compared to red and brown seaweeds, fewer studies have focused on the biological activity of green seaweed’s oligosaccharides. This study aimed to produce bioactive ulvan oligosaccharides via enzymatic hydrolysis from green seaweed Ulva lactuca. Ulvan, a water-soluble polysaccharide, was obtained by hot water extraction. Two isolated marine bacteria, Pseudomonas vesicularis MA103 and Aeromonas salmonicida MAEF108, were used to produce multiple hydrolases, such as ulvanolytic enzymes, amylase, cellulase, and xylanase, to degrade the ulvan extract. An ultrafiltration system was used to separate the enzymatic hydrolysate to acquire the ulvan oligosaccharides (UOS). The characteristics of the ulvan extract and the UOS were determined by yield, reducing sugar, uronic acid, sulfate group, and total phenols. The FT-IR spectrum indicated that the ulvan extract and the UOS presented the bands associated with O-H, C=O, C-O, and S=O stretching. Angiotensin I converting enzyme (ACE) inhibition and antioxidant activities in vitro were evaluated in the ulvan extract and the UOS. These results provide a practical approach to producing bioactive UOS by microbial enzymatic hydrolysis that can benefit the development of seaweed-based products at the industrial scale.

Study on Subcritical Water Extraction of Flavonoids from Scutellaria baicalenses

2013 ◽  
Vol 662 ◽  
pp. 293-296 ◽  
Author(s):  
Yan Cheng ◽  
Jing Liu ◽  
Chang An Liu
Keyword(s):  
Subcritical Water ◽  
Extraction Yield ◽  
Water Extraction ◽  
Extraction Time ◽  
Particle Sizes ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Operation Conditions ◽  
Conventional Extraction ◽  
Total Extraction

The extraction of baicalin, baicalein, wogonin and wogonoside from Scutellaria Baicalenses was performed using subcritical water. The effects of key operation conditions by varying the temperature (110-160 °C), extraction time (10-90 min), water loadings (2.0-4.0 mL)and particle sizes (>20- <100 mesh) were evaluated. The highest extraction yields of baicalin and wogonoside were obtained at extraction temperature of 160 °C, extraction time of 60 min, water loadings of 4.0 mL and 60-100 mesh. The highest extraction yields of baicalein and wogonin were obtained at extraction temperature of 110 °C, extraction time of 10 min, water loadings of 4.0 mL and 60-100 mesh. The subcritical water extraction was compared with the conventional extraction method. The total extraction yield by SCWE was higher than those obtained using ethanol as the solvent.

scholarly journals Evaluation of the Extraction Temperature Influence on Polyphenolic Profiles of Vine-Canes (Vitis vinifera) Subcritical Water Extracts

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 872
Author(s):  
Olena Dorosh ◽  
Manuela M. Moreira ◽  
Diana Pinto ◽  
Andreia F. Peixoto ◽  
Cristina Freire ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Phenolic Content ◽  
Subcritical Water ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Oxygen Species ◽  
Subcritical Water Extraction ◽  
Major Class ◽  
In Vitro Effects

This work focused on evaluating the possibility of using vineyard pruning wastes from two Portuguese Vitis vinifera varieties; Touriga Nacional (TN) and Tinta Roriz (TR), as new potential ingredients for the nutraceutical industry. An environmentally friendly extraction technique; namely subcritical-water extraction (SWE), was employed. The overall results indicate that phenolic acids were the major class of compounds quantified; being gallic acid the principal one. The highest value for total phenolic content (TPC) was obtained for the TR extract at 250 °C (181 ± 12 mg GAE/g dw). In terms of antioxidant activity; the DPPH values for the extracts obtained at 250 °C were approximately 4-fold higher than the ones obtained at 125 °C; with TR extract presenting the highest value (203 ± 22 mg TE/g dw). Thus, the TR extract obtained through SWE at 250 °C was selected to evaluate the scavenging activity and the in vitro effects on cells due to the best results achieved in the previous assays. This extract presented the ability to scavenge reactive oxygen species (O2●-, HOCl and ROO●). No adverse effects were observed in HFF-1 viability after exposure to extract concentrations below 100 μg/mL. This work demonstrated that vine-canes extracts could be a potential ingredient to nutraceutical industry

scholarly journals Comparing of hot water and acid extraction of polysaccharides from proso millet

2017 ◽  
Vol 23 (1) ◽  
pp. 141-150
Author(s):  
Aishi Zhu ◽  
Shanshan Liu ◽  
Kanfeng Wu ◽  
Chuan Ren ◽  
Maoqian Xu
Keyword(s):  
Polynomial Equation ◽  
Hot Water ◽  
Water Extraction ◽  
Acid Extraction ◽  
Extraction Temperature ◽  
Hot Water Extraction ◽  
Solid Ratio ◽  
Proso Millet ◽  
Acid Aqueous Solution ◽  
Experimental Yield

The extraction of polysaccharides from proso millet was investigated experimentally using hot water and acid aqueous solution. Response surface methodology, based on a three-level, three- or four-variable Box-Behnken design for hot water extraction or acid extraction, respectively, was employed to obtain the best possible combination of acid concentration, liquid-solid ratio, extraction time, and extraction temperature for maximum polysaccharides yield. The obtained experimental data were fitted to a second-order polynomial equation and analyzed by appropriate statistical methods. The corresponding optimum extraction conditions of each method were obtained. Under the optimum conditions, the experimental yield was well in close agreement with the predicted value by the model. The results showed that the polysaccharides yield of acid extraction was 42.13 mg g-1, significantly higher than 20.07 mg g-1 of the yield of hot water extraction, the obtained equation could be used to predict the extraction experimental results.

scholarly journals SubcriticalWater Extraction and Determination of Nifedipine in Pharmaceutical Formulations

2006 ◽  
Vol 89 (2) ◽  
pp. 365-368 ◽  
Author(s):  
Pablo Richter ◽  
Maria Ines Toral ◽  
Carla Toledo
Keyword(s):  
Subcritical Water ◽  
Pharmaceutical Formulations ◽  
Hot Water ◽  
Content Uniformity ◽  
Extraction Temperature ◽  
Subcritical Water Extraction ◽  
Quantitative Extraction ◽  
Relative Standard ◽  
Dynamic Extraction

Abstract A rapid and simple continuous method for the extraction of nifedipine from tablets was developed by using pressurized hot water at 150C. This is the first time that subcritical water was applied to the extraction of low-polarity compounds in pharmaceutical analysis. The method is based on the increment in solubility of nifedipine in subcritical water. Extraction temperature and static and dynamic extraction time were optimized in order to reach quantitative extraction of the drug from the tablets. After extraction, the drug was determined by spectrophotometry by measuring absorbance at 338 nm. Accuracy and precision of themethodwere determined by analysis of 10 synthetic samples of pharmaceutical formulations preparedwith common tablet excipients. Recoverywas found to be 99.2with a relative standard deviation of 1.9, which indicates that the excipients of the formulation do not interfere in the determination. The method was applied to the determination of the drug content uniformity in tablets.

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