Pengaruh Penyimpanan Beku terhadap Karakteristik Kimia dan Fisik Caulerpa racemosa, Drip, dan Filtratnya

Caulerpa racemosa merupakan rumput laut hijau dengan kandungan air yang tinggi sehingga mudah mengalami kerusakan. Penyimpanan dalam suhu beku merupakan salah satu cara untuk memperpanjang masa simpan suatu produk. Namun, pembekuan produk juga dapat menyebabkan terjadinya berbagai perubahan yang tidak dikehendaki. Penelitian ini bertujuan untuk menganalisis pengaruh penyimpanan pada suhu beku terhadap karakteristik kimia dan fisik C. racemosa, drip, dan filtrat yang dihasilkan. Sampel berupa C. rasemosa disimpan dalam ruangan penyimpan beku (cold storage) dan diamati setiap minggunya hingga minggu ke-4. Parameter uji yang diamati meliputi rendemen, komposisi proksimat, kadar garam, uji warna (L*, a*, b*), dan viskositas, masing-masing pada C. racemosa yang ditiriskan, drip, dan filtrat C. racemosa. Hasil penelitian menunjukkan bahwa penyimpanan beku C. racemosa dapat mengurangi berat pada sampel akhir karena sebagian besar drip keluar setelah pelelehan/thawing, yaitu sekitar 77,88-84,81%. Karakteristik fisik maupun kimia C. racemosa yang disimpan selama 1 hingga 4 minggu tidak berbeda nyata antar waktu penyimpanan, namun berbeda nyata pada C. racemosa tanpa disimpan beku, yaitu pada kadar air, abu, dan garam.ABSTRACTCaulerpa racemosa is a green seaweed that has a high moisture content, so it is easily damaged. Frozen storage is one way to extend the shelf life of a product. However, freezing the product can cause various undesirable changes to occur. This study was aimed to analyze the effect of storage at freezing temperature on the chemical and physical characteristics of C. racemosa, drip, and the resulting filtrate. C. racemosa samples were stored in cold storage and observed every week until the 4th week. The test parameters observed were yield, proximate composition, salt content, color (L*, a*, b*), and viscosity on drained C. racemosa, drip, and C. racemosa filtrate. The results showed that frozen storage of C. racemosa could cause weight loss in the final sample because most of the drip came out after thawing, which was around 77.88 - 84.81%. Physical and chemical characteristics of C. racemosa stored for 1 to 4 weeks were not significantly different from unfrozen C. racemosa, i.e. water, ash, and salt content.

Molecular characterization of Caulerpa racemosa (Caulerpales, Chlorophyta) from Indonesia based on the Plastid tufA Gene

The green seaweed Caulerpa racemosa is a seaweed of high prospect that is being given emphasis by the Indonesian government. However, C. racemosa in Indonesia may include multiple species level-entity exhibiting morphological overlap and require molecular analysis for species identification. Molecular documentation of species richness of indigenous populations of C. racemosa is essential to underpin cultivar development and conservation of the species to avoid overharvesting. The present study aimed to determine the genetic diversity of C. racemosa and document the haplotype network of the specimens from four different locations (Bintan Island, Jepara, Takalar and Osi Island) using the chloroplast tufA gene. Twenty individuals from four areas were collected and amplified with the chloroplast-encoded gene tufA for species identification against publicly available data. The identification of C. racemosa based on the tufA gene showed that the species found in four locations were C. cylindracea (previously C. racemosa var. cylindracea), C. macra (previously C. racemosa var. macra), C. racemosa, and C. oligophylla (previously C. racemosa var. lamourouxii). This study records the existence of C. cylindracea in Takalar and Jepara, Indonesia for the first time. The most diverse C. racemosa species was in Osi Island, where the exploitation of this seaweed is very low. In contrast, the lowest number of C. racemosa varieties were found in Takalar, where exploitation is very high. There were only minor light variations of Caulerpa species in the tufA gene in four different sites with only four haplotypes found, and each haplotype corresponded to another species.

Characterization of the physical properties and sensory acceptability of Caulerpa racemosa grain beverage

Caulerpa racemosa grain was a healthy product and high nutrition. This study applied study was applied Caulerpa racemosa grain in the beverage with the addition of alginate powder. Thus, the present work was aimed to determine the properties of beverage C. racemosa grain formulated with the alginate concentration was varied (0; 0.025; 0.05; 0.075; 0.10 and 0.125 %). The testing of quality beverage C. racemosa viscosity was analyzed using viscometer methods. The colorimeter investigated the colour. The sensory acceptability hedonic and score method (colour, appearance, aroma, taste, texture, and overall acceptance) used a 5-point. Results showed that alginate used alginate 0.025%, the highest preference, aroma, and appearance scores. The addition of alginate increased alginate was increased the viscosity of beverage C. racemosa grain. The colour of C. racemosa grain progressively became light as the level of alginate grew. However, the increased alginate is not significant enough to change the redness and greenness value of C. racemosa grain beverage. The panelists have preferred to drink the selected Caulerpa grain beverage (concentration sodium alginate 0.025%), with slightly viscous.

The Fortification Effects of Sea Grapes (Caulerpa racemosa) Powder on Color and Sensory of Hakau Dim Sum Wrappers

Sea grapes (Caulerpa racemosa) are green seaweeds that include containing dietary fiber, antioxidant compounds, and natural dyeing colours such as chlorophyll and carotenoid pigments. Sea grapes’ green tint, when ground into powder, has the potential to be used as a coloring agent. It can be used as an alternative dyeing agent and product diversification on hakau dim sum wrappers. The purpose of this study was to determine how adding sea grapes powder affected the physical properties (color and folding test) and sensory attributes (time-intensity) of hakau dim sum wrappers. The research was conducted by manufacturing sea grape powder and the wrappers by hand. Caulerpa racemosa was added in various concentration (0%; 1.5%; 3%; 4.5%; and 6%) (w/w). The dough was molded with a thickness of no.5 pasta maker with 8 cm diameter. The wrappers were physically tested and steamed for 15 minutes for sensory testing. As a result, wrappers fortified with sea grape powder show green colour and stand up the transparent characteristics. Lightness (L*) and redness (a*) decreased with increasing concentration of sea grape powder which indicated dark green wrappers color. Sea grape powder affected raw wrappers in the folding test. Time-intensity evaluation looked at the gelatinized flour flavor and sea grapes flavor. The result shows the gelatinized flour flavor reached the highest peak in the control wrappers, while the concentration of 6% had the highest intensity of sea grapes flavor and the lowest gelatinized flour flavor. In conclusion, the addition of sea grape powder resulted in a transparent green dim sum wrapper, decreased folding score for raw and the sea grapes flavor was more dominant than the gelatinized flour flavor.

Nutritional Value and Biofunctionalities of Two Edible Green Seaweeds (Ulva lactuca and Caulerpa racemosa) from Indonesia by Subcritical Water Hydrolysis

Caulerpa racemosa (sea grapes) and Ulva lactuca (sea lettuces) are edible green seaweeds and good sources of bioactive compounds for future foods, nutraceuticals and cosmeceutical industries. In the present study, we determined nutritional values and investigated the recovery of bioactive compounds from C. racemosa and U. lactuca using hot water extraction (HWE) and subcritical water extraction (SWE) at different extraction temperatures (110 to 230 ºC). Besides significantly higher extraction yield, SWE processes also give higher protein, sugar, total phenolic (TPC), saponin (TSC), flavonoid contents (TFC) and antioxidant activities as compared to the conventional HWE process. When SWE process was applied, the highest TPC, TSC and TFC values were obtained from U. lactuca hydrolyzed at reaction temperature 230 ºC with the value of 39.82 ± 0.32 GAE mg/g, 13.22 ± 0.33 DE mg/g and 6.5 ± 0.47 QE mg/g, respectively. In addition, it also showed the highest antioxidant activity with values of 5.45 ± 0.11 ascorbic acid equivalents (AAE) mg/g and 8.03 ± 0.06 trolox equivalents (TE) mg/g for ABTS and total antioxidant, respectively. The highest phenolic acids in U. lactuca were gallic acid and vanillic acid. Cytotoxic assays demonstrated that C. racemosa and U. lactuca hydrolysates obtained by HWE and SWE did not show any toxic effect on RAW 264.7 cells at tested concentrations after 24 h and 48 h of treatment (p < 0.05), suggesting that both hydrolysates were safe and non-toxic for application in foods, cosmeceuticals and nutraceuticals products. In addition, the results of this study demonstrated the potential of SWE for the production of high-quality seaweed hydrolysates. Collectively, this study shows the potential of under-exploited tropical green seaweed resources as potential antioxidants in nutraceutical and cosmeceutical products.

PENURUNAN KONSENTRASI KLOROFIL KRIM SUP CAULERPA RACEMOSA YANG DIKERINGKAN DENGAN VACUUM DRYING OVEN

Caulerpa racemosa adalah salah satu spesies alga hijau yang dapat dimanfaatkan sebagai bahan baku krim sup. Nilai tambah krim sup tersebut adalah adanya kandungan klorofil sebagai senyawa bioaktif dan sebagai pewarna alami. Permasalahn yang terjadi adalah klorofil tidak tahan terhadap panas pada saat pengolahan. Vacuum drying oven menjadi solusi untuk mengurangi kerusakan klorofil akibat pemanasan. Tujuan penelitian ini adalah mengetahui pengaruh suhu terhadap kandungan klorofil pada proses pengeringan krim sup yang terbuat dari Caulerpa racemosa dengan menggunakan vacuum drying oven. Tahapan penelitian ini meliputi, pembuatan krim sup, pengeringan, ekstraksi klorofil, dan analisis klorofil. Hasil penelitian ini diperoleh empat variasi suhu yakni 60, 70,80, dan 90ºC. Kandungan klorofil paling tinggi pada pengeringan dengan suhu 60ºC, dengan komposisi klorofil a (2,0786 µg/mL), klorofil b (1,8742 µg/mL), dan total klorofil (3,9528 µg/mL). Paparan panas menyebabkan klorofil menjadi tidak stabil. Berdasarkan data yang diperoleh dapat disimpulkan suhu optimal dalam mengeringkan krim sup dengan vacuum drying oven adalah 60ºC.

Potential of Ulva linza L. and Caulerpa racemosa var. uvifera seaweeds from Cox’s Bazar, Bangladesh as sea vegetable

The nutritional quality parameters of leafy Ulva linza L. collected from the west coast of the Naf river estuary, Teknaf and grape-like Caulerpa racemosa var. uvifera collected from St. Martin's Island, Cox's Bazar, Bangladesh, were studied. Total protein content in U. linza (22.05±1.73%) was found significantly (p<0.05) higher than C. racemosa var. uvifera (18.20±1.50%) but with low crude lipids in the former; total amino acid content was also higher in the former, both having nearly the same amount of essential and non-essential amino acids; vitamin C content (about 2.5 mg/100 g) was nearly identical but β -carotene content was higher in the later (9.04 mg/100 g dry wt.). Crude fiber in U. linza was low (4.21±0.43%) but high in C. racemosa var. uvifera (18.20±1.50%). Other micronutrients were also ascertained. The nutritional quality parameters determined suggested that the “sea-salad” and “sea- grapes” can be used as sea-vegetables and food supplements. Bangladesh J. Sci. Ind. Res.56(3), 223-230, 2021

The effect of NPK fertilizer with different dosage on the growth rate seaweed (Caulerpa racemosa)

NPK is a type of fertilizer that plays a role in increasing growth and survival in plants such as seaweed. This study aims to determine the effect of different NPK fertilizer doses on the growth rate of seaweed (Caulerpa racemosa), and to determine which NPK fertilizer dosage has the best effect on the growth rate of C. racemosa. Research begins with collecting samples of seaweed in coastal waters. Ternate Island District Kastela, and the cultivation process is carried out on Jalan Jan, Tabona Village, South Ternate City. The time of the research was two months from August to October, 2020. This study used 12 units of cool box in the form of cork with a size of 90 x 30 cm, which is used as a container for maintaining of C. racemosa. In each treatment using a seed weight of 50 grams. The NPK fertilizer dosage treatment tested was 4 doses, with 3 replications, namely: treatment A: 40 ml NPK fertilizer; B: 60 ml NPK fertilizer; C: 80 ml NPK fertilizer; D: 0 ml NPK fertilizer (control). Research containers using random placement. The design used was a completely randomized design (CRD), using analysis of variance (ANOVA). The results obtained showed that NPK fertilizer with different doses the effect is not significantly different on the growth rate of C. racemosa with the highest average growth rate in treatment C amounting to 8.725%, followed by treatment B of 8.178%, treatment A of 7.761%, and the lowest was treatment D of 6.519%.Keywords:NPKSeaweedCaulerpa racemosaGrowth rate