Identification of a Novel 2S Albumin with Antitryptic Activity from Caryocar brasiliense Seeds

Chickpea is a key crop in sustainable cropping systems and for its nutritional value. Studies on agronomic and genetic influences on chickpea protein composition are missing. In order to obtain a deep insight into the genetic response of chickpeas to management in relation to agronomic and quality traits, a two-year field trial was carried out with eight chickpea genotypes under an organic and conventional cropping system. Protein composition was assessed by SDS-PAGE in relation to the main fractions (vicilin, convicilin, legumin, lectin, 2s-albumin). Crop response was highly influenced by year and presumably also by management, with a −50% decrease in grain yield under organic farming, mainly due to a reduction in seed number per m2. No effect of crop management was observed on protein content, despite significant differences in terms of protein composition. The ratio between the major globulins, 7s vicilin and 11s legumin, showed a negative relationship with grain yield and was found to be higher under organic farming. Among genotypes, black-seed Nero Senise was characterized by the highest productivity and water-holding capacity, associated with low lectin content. These findings highlight the importance of the choice of chickpea genotypes for cultivation under organic farming in relation to both agronomic performance and technological and health quality.

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Optimization of lipase extraction from pequi seed ( Caryocar brasiliense Camb.)

Journal of Food Processing and Preservation ◽

10.1111/jfpp.15616 ◽

2021 ◽

Author(s):

Priscilla Amaral Nascimento ◽

Annie Nolasco Alves ◽

Karine Amaral dos Santos ◽

Cristiane Martins Veloso ◽

Leandro Soares Santos ◽

...

Keyword(s):

Caryocar Brasiliense

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Vicilin and legumin storage proteins are abundant in water and alkali soluble protein fractions of glandless cottonseed

Scientific Reports ◽

10.1038/s41598-021-88527-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zhongqi He ◽

Christopher P. Mattison ◽

Dunhua Zhang ◽

Casey C. Grimm

Keyword(s):

Mass Spectrometry ◽

Soluble Protein ◽

Extraction Efficiency ◽

Storage Proteins ◽

Protein Composition ◽

Protein Fractions ◽

Peptide Fragments ◽

2S Albumin ◽

Cottonseed Protein ◽

Glandless Cottonseed

AbstractIn this work, we sequentially extracted water (CSPw)- and alkali (CSPa)-soluble protein fractions from glandless cottonseed. SDS-Gel electrophoresis separated CSPw and CSPa to 8 and 14 dominant polypeptide bands (110–10 kDa), respectively. Liquid chromatography-electrospray ionization-tandem mass spectrometry identified peptide fragments from 336 proteins. While the majority of peptides were identified as belonging to vicilin and legumin storage proteins, peptides from other functional and uncharacterized proteins were also detected. Based on the types (unique peptide count) and relative abundance (normalized total ion current) of the polypeptides detected by mass spectrometry, we found lower levels (abundance) and types of legumin isoforms, but higher levels and more fragments of vicilin-like antimicrobial peptides in glandless samples, compared to glanded samples. Differences in peptide fragment patterns of 2S albumin and oleosin were also observed between glandless and glanded protein samples. These differences might be due to the higher extraction recovery of proteins from glandless cottonseed as proteins from glanded cottonseed tend to be associated with gossypol, reducing extraction efficiency. This work enriches the fundamental knowledge of glandless cottonseed protein composition. For practical considerations, this peptide information will be helpful to allow better understanding of the functional and physicochemical properties of glandless cottonseed protein, and improving the potential for food or feed applications.

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Pollination ecology of Caryocar brasiliense (Caryocaraceae) in Central Brazil cerrado vegetation

Journal of Tropical Ecology ◽

10.1017/s0266467400007173 ◽

1993 ◽

Vol 9 (2) ◽

pp. 199-211 ◽

Cited By ~ 55

Author(s):

Rogério Gribel ◽

John D. Hay

Keyword(s):

Breeding System ◽

Fruit Set ◽

Seed Set ◽

Floral Biology ◽

Cerrado Vegetation ◽

Central Brazil ◽

Glossophaga Soricina ◽

Caryocar Brasiliense ◽

Anoura Geoffroyi ◽

Phyllostomus Discolor

ABSTRACTThe floral biology, breeding system and pollination of Caryocar brasiliense were studied in the cerrado vegetation of Central Brazil. The large, yellowish-cream, brush-like flowers are pollinated mainly by glossophagine bats (Glossophaga soricina and Anoura geoffroyi). Three non-glossophagine bats (Phyllostomus discolor, Vampyrops lineatus and Carollia perspicillata) and two short probosisced hawk moths (Erinyis ello and Pseudosphinx tetrio) may also act as occasional pollinators. Caryocar brasiliense is self-compatible although it sets significantly more fruits when crossed than when selfed. The natural fruit set (fruit/flower ratio) and seed set (seed/ovule ratio) are 3.1% and 1.0% respectively. Most of the fruits and seeds are formed through the action of the flower visitors, despite the fact that about 20% of the non-visited flowers receive self pollen on at least one stigma.

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Traditional knowledge and uses of the Caryocar brasiliense Cambess. (Pequi) by “quilombolas” of Minas Gerais, Brazil: subsidies for sustainable management

Brazilian Journal of Biology ◽

10.1590/1519-6984.22914 ◽

2016 ◽

Vol 76 (2) ◽

pp. 511-519 ◽

Cited By ~ 10

Author(s):

L. C. L. Pinto ◽

L. M. O. Morais ◽

A. Q. Guimarães ◽

E. D. Almada ◽

P. M. Barbosa ◽

...

Keyword(s):

Participant Observation ◽

Minas Gerais ◽

Structured Interviews ◽

Alternative Source ◽

Soap Production ◽

Community Data ◽

Caryocar Brasiliense ◽

Ecological Importance ◽

Support Of Research ◽

Caryocar Brasiliense Cambess

Abstract Local knowledge of biodiversity has been applied in support of research focused on utilizing and management of natural resources and promotion of conservation. Among these resources, Pequi (Caryocar brasiliense Cambess.) is important as a source of income and food for communities living in the Cerrado biome. In Pontinha, a “quilombola” community, which is located in the central region of State of Minas Gerais, Brazil, an ethnoecological study about Pequi was conducted to support initiatives for generating income for this community. Data were collected through semi-structured interviews, participant observation, and crossing. The most relevant uses of Pequi were family food (97%), soap production (67%), oil production (37%), medical treatments (17%), and trade (3%). Bees were the floral visitors with the highest Salience Index (S=0.639). Among frugivores that feed on unfallen fruits, birds showed a higher Salience (S=0.359) and among frugivores who use fallen fruits insects were the most important (S=0.574). Borers (folivorous caterpillars) that attack trunks and roots were the most common pests cited. According to the respondents, young individuals of Pequi are the most affected by fire due to their smaller size and thinner bark. Recognition of the cultural and ecological importance of Pequi has mobilized the community, which has shown interest in incorporating this species as an alternative source of income.

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