scholarly journals Major Antifungals in Nutmeg Essential Oil against Aspergillus flavus and A. ochraceus

2014 ◽  
Vol 4 (1) ◽  
pp. 51 ◽  
Author(s):  
Vania Maria Moreira Valente ◽  
Gulab Newandram Jham ◽  
Carolina Marangon Jardim ◽  
Onkar Dev Dhingra ◽  
Ion Ghiviriga
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Aspergillus Flavus ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Storage Fungi ◽  
Pure Compounds ◽  
Nutmeg Essential Oil

<p>Aiming to substitute toxic synthetic fungicides, the activity of nutmeg (<em>Myristica fragrans</em>) essential oil (EO, obtained by hydrodistillation) was investigated against two important storage fungi-<em>Aspergillus flavus</em> <em>A. ochraceus</em>. The activity of crude nutmeg EO was investigated using poison food assay (PFA). At a concentration of 0.1%, the EO inhibited <em>A. flavus</em> and <em>A. ochraceus</em> growth by 43 and 65%, respectively. At a concentration of 0.3 %, <em>A. flavus</em> and <em>A. ochraceus</em> inhibitions were 84 and 79%, respectively. The crude nutmeg EO on fractionation by preparative TLC-bioautography presented one band from which two pure compounds were isolated by semi-preparative normal-phase high performance liquid chromatography. Myristicin and safrole were identified by nuclear magnetic resonance (<sup>1</sup>H and <sup>13</sup>C) and gas chromatography-mass spectrometry. The relative % of myristicin and safrol in the crude EO was 10.8 and 2.9, respectively, determined by gas chromatography with a flame ionization detector. The crude EO, the isolated active fraction, isolated myristicin and standard myristicin presented similar activities against the two fungi at concentrations of 0.1 and 0.3% by PFA. Based on these results it is concluded that myristicin is the major antifungal in nutmeg EO against <em>A. flavus</em> and <em>A. ochraceus</em>.</p>

Chemical Composition of the Essential Oil From Minthostachys acris Schmidt-Leb. Grown in the Peruvian Andes

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985749
Author(s):  
Jorge A. Pino ◽  
María Milagros Dueñas-Mendoza ◽  
Leoncio Solís-Quispe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Steam Distillation ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Peruvian Andes ◽  
Flame Ionization ◽  
Aerial Parts

The chemical composition of the essential oil from aerial parts of Minthostachys acris Schmidt-Leb. grown in Cuzco was studied. A total of 59 volatile compounds were identified by gas-chromatography-flame ionization detector and gas chromatography-mass spectrometry in the essential oil obtained by steam distillation, of which the most prominent were pulegone (54.4%), cis-menthone (11.0%), and thymol (6.3%).

scholarly journals Composition of the essential oil of Satureja metastasiantha: a new species for the flora of Turkey

2020 ◽  
Vol 75 (7-8) ◽  
pp. 271-277
Author(s):  
Sema Carikci ◽  
Ahmet C. Goren ◽  
Tuncay Dirmenci ◽  
Burhanettin Yalcinkaya ◽  
Aysen Erkucuk ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Ionization Detector ◽  
Flora Of Turkey ◽  
Flame Ionization ◽  
Aerial Parts ◽  
A New Species ◽  
Type Apparatus

AbstractThe aerial parts of Satureja metastasiantha were hydrodistilled for 3 h using a Clevenger-type apparatus. The essential oils were analyzed by gas chromatography/flame ionization detector and gas chromatography/mass spectrometry, simultaneously, the main compounds of which were characterized as p-cymene (22.3%), thymol (21.0%), carvacrol (18.4%), and γ-terpinene (12.1%). Antioxidant capacity, acetylcholinesterase and butyrylcholinesterase inhibition effects, and antimicrobial and antifungal properties of the species were evaluated. The anticholinesterase activity of the essential oil of S. metastasiantha was observed with 30% inhibition at 200 μg/mL. The essential oil of the species showed activity against Staphylococcus aureus with 128 μg/mL minimum inhibitory concentration value.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oil From the Leaves of Pterocephalus hookeri

2020 ◽  
Vol 15 (12) ◽  
pp. 1934578X2098123
Author(s):  
Peng-fei Yang ◽  
Hui Lu ◽  
Qiong-bo Wang ◽  
Zhi-wei Zhao ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
And Staphylococcus Aureus ◽  
Antimicrobial Assay ◽  
Detailed Chemical

Detailed chemical constituents of essential oil from the Pterocephalus hookeri leaves and its antimicrobial activities were investigated in this study. The essential oil, obtained by hydrodistillation, was characterized by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analyses. Among the 90 identified compounds, hexadecanoic acid (21.27%), phytol (8.03%), furfural (7.08%), oleic acid (5.25%), and phytone (4.56%) were the major components. In the antimicrobial assay, the essential oil showed strong inhibitory activities against Escherichia coli, Candida albicans, and Staphylococcus aureus with minimum inhibitory concentration values of 31.3, 62.5, and 125 µg/mL, respectively. To our knowledge, this is the first report concerning chemical composition and antimicrobial activities of the essential oil from Pterocephalus hookeri.

scholarly journals Chemical composition of the essential oil of Psidium guajava leaves and its toxicity against Sclerotinia sclerotiorum

2018 ◽  
Vol 39 (2) ◽  
pp. 865
Author(s):  
Elizabeth Aparecida Josefi Silva ◽  
Vanessa Paula da Silva ◽  
Cassia Cristina Fernandes Alves ◽  
José Milton Alves ◽  
Edson Luiz Souchie ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Sclerotinia Sclerotiorum ◽  
Psidium Guajava ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Randomized Design ◽  
Dry Seasons ◽  
Guava Leaves

Globally, the fungus Sclerotinia sclerotiorum is considered as one of the most important phytopathogens, since it affects the production of several economically important crops. Further, it is difficult to control, thus increasing the use of pesticides. Therefore, the search for new substances, especially those extracted from plants, has received special attention to control this plant pathogen. The objective of the present study was to evaluate the yield and chemical composition of the essential oil extracted from the fresh leaves of guava collected during the rainy and dry seasons, and also to evaluate its toxicity against S. sclerotiorum. The experiment was a completely randomized design with five treatments and six replicates. The essential oil was extracted by hydrodistillation of the leaves and analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The main compounds identified in the essential oil were trans-caryophyllene and ?-humulene. The essential oil at a concentration of 300 ?L exhibited 90% inhibition of the mycelial growth of S. sclerotiorum. This confirmed the antifungal potential of the essential oil of the guava leaves during both the sampling seasons.

scholarly journals Chemical Composition of Vernonia albicans Essential Oil from India

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil

The chemical composition of the hydro-distilled essential oil obtained from the flowering aerial parts of Vernonia albicans DC. (Asteraceae) was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with a mass spectrometry (GC/MS). Thirty-nine compounds have been identified, representing 97.5% of the total oil. The major constituents were β-caryophyllene (34.3%), γ-amorphene (19.5%), 9-epi-β-caryophyllene (6.9%), and α-pinene (6.9%). The oil was found to be rich in sesquiterpene hydrocarbons (73.9%).

scholarly journals Essential Oil Constituents of Etlingera yunnanensis and Hornstedtia sanhan grown in Vietnam

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Dao T. M. Chau ◽  
Do N. Dai ◽  
Tran M. Hoi ◽  
Tran H. Thai ◽  
Tran D. Thang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Ionization Detector ◽  
Methyl Chavicol ◽  
Flame Ionization ◽  
Germacrene D ◽  
Leaf Oil ◽  
Different Parts

The chemical composition of the essential oils obtained from the hydrodistillation of different parts of Etlingera yunnanensis (T.L. Wu &S.J. Chen) R.M. Sm. and Hornstedtia sanhan M.F. Newman (Zingiberaceae) grown in Vietnam are reported. The analysis was performed by means of a gas chromatography-flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). The essential oil of the leaves of E. yunnanensis comprised mainly germacrene D (19.2%), β-pinene (11.6%), and α-amorphene (11.2%), while that of the stems was rich in β-pinene (23.7%), 1,8-cineole (11.0%) and α-pinene (9.6%). The major components of the root oil of E. yunnanensis were β-pinene (31.9%), α-pinene (13.7%) and 1,8-cineole (9.4%). However, α-pinene (25.9%, 36.3% and 14.1% for leaves, stems and roots, respectively), β-pinene (9.7%, 11.8% and 7.2% for leaves, stems and roots, respectively) and limonene (22.1%, 24.6% and 16.2% for leaves, stems and roots, respectively) were the major compounds of H. sanhan. In addition, α-humulene (15.9%) was present in larger amounts in the leaf oil while the root oil contained the largest proportion of methyl chavicol (43.7%).

scholarly journals Compositional and Enantiomeric Analysis of the Essential Oil of Taxodium Distichum from India

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100
Author(s):  
Rajendra C. Padalia ◽  
Ram S. Verma ◽  
Amit Chauhan ◽  
Prakash Goswami ◽  
Chandan S. Chanotiya
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Taxodium Distichum ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Flame Ionization ◽  
Enantiomeric Analysis ◽  
Racemic Form ◽  
Different Seasons

The leaf essential oil composition of Taxodium distichum L., collected from the foothills of Uttarakhand, India was analyzed using gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) equipped with DB-5 (5% diphenyl-95% dimethyl polysiloxane) and β-cyclodextrin (6-tertiarybutyldimethylsiliyl-2,3-diethyl-β-cyclodextrin) capillary columns. Seventeen constituents, representing 90.3 to 99.4% of composition were identified in the essential oils from different seasons, viz. spring, summer, rainy, autumn and winter. The essential oil composition was mainly dominated by monoterpene hydrocarbons, represented mainly by α-pinene (81.9–94.3%). Other constituents of the oil were myrcene (0.5–4.7%), β-pinene (2.2–2.9%), limonene (0.5–1.5%), camphene (≤0.03–1.5%), and α-terpineol (upto 1.6%). Chiral analysis of T. dstichum essential oil on an ethyl substituted β-cyclodextrin capillary column revealed the presence of α-pinene in racemic form, with an enantiomeric ratio of 49.3% for (1 R)-(+)- and 50.7% for (1 S)-(-)-α-pinene.

scholarly journals Chemical Composition of the Essential Oil of Lepidagathis Fasciculata from Bondla Forest of Goa, India

2013 ◽  
Vol 8 (8) ◽  
pp. 1934578X1300800
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D ◽  
Total Oil

The hydro-distilled essential oil obtained from the flowering aerial parts of Lepidagathis fasciculata Nees was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC/MS). A total of 38 compounds have been identified, representing 91.2% of the total oil. The major constituents were δ-cadinene (14.4 %), γ-curcumene (9.8%), sandaracopimarinal (6.6%), germacrene D-4-ol (6.1%), cembrene (5.0%), β-calacorene (3.6%), ar-curcumene (3.3%), trans–4,10-epoxy-amorphane (3.2%), abietatriene (2.9%), and α-cubebene (2.8%). The oil was rich in sesquiterpene hydrocarbons (43.8%).

scholarly journals Chemical Constituents of Essential Oils from Resin and Bark of Agathis borneensis

1970 ◽  
Vol 2 (1) ◽  
pp. 28-32
Author(s):  
Fouziah Binti Alet ◽  
Zaini Bin Assim ◽  
Ismail Bin Jusoh ◽  
Fasihuddin Badruddin Ahmad
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Mass Spectrometer ◽  
Capillary Gas Chromatography ◽  
Chemical Constituents ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Main Components

The chemical constituents of essential oils obtained from resin and bark of Agathis borneensis were analysed using capillary gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometer (GC/MS). The resin essential oil dominated by α-pinene (30.93%), δ-limonene (17.79%), β-pinene (11.28%) and terpinen-4-ol (8.35%). The main components in the bark essential oil were β-pinene (8.68%), terpinen-4-ol (8.54%), α-pinene (8.50%) and α-terpineol (8.48%).

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