scholarly journals Evaluation of Protein Nutritional Quality of Four Soybean Varieties Grown in Western Kenya

2018 ◽  
Vol 7 (5) ◽  
pp. 69 ◽  
Author(s):  
Fredrick Agengo ◽  
Charlotte Serrem ◽  
Florence Wamunga
Keyword(s):  
Amino Acid ◽  
Nutritional Quality ◽  
Protein Energy Malnutrition ◽  
Protein Digestibility ◽  
Western Kenya ◽  
Protein Retention ◽  
Protein Energy ◽  
Amino Acid Score ◽  
True Protein

Protein nutrition is important for human health because its deficiency leads to major public health problems such as Protein Energy Malnutrition. Soybean an excellent and cheap source of high quality protein has been introduced into the Western Kenya and other developing countries for improved health and food security, but end use qualities may reduce its utilization. The main objective of this study was to evaluate the protein nutritional quality of four soybean varieties grown in Western Kenya. Protein quality was determined using male weanling albino rats for the indices of net protein retention, food efficiency, apparent and true protein digestibilities and faecal weight. Soybean amino acid efficiency was evaluated using Protein digestibility corrected amino acid score (PDCAAS). Soybean diet SB 132 had the highest protein nutritional quality with the best protein retention of 6.29 g, Apparent Protein digestibility of 89.13%, True Protein Digestibility of 96.48%, weight gain of 5.50 g and a Net Protein Retention Ratio of 4.70. All the four soybean varieties had high amino acid profiles with a Protein digestibility Corrected Amino Acid Score of 1.0. Soybean variety SB 132 is the most superior in digestibility and the study recommends its promotion as a food crop in Western Kenya and other developing countries for the management of Protein Energy Malnutrition and for food security.

scholarly journals Rat Bioassay for Evaluation of Protein Quality of Soy-Fortified Complementary Foods

2017 ◽  
Vol 6 (6) ◽  
pp. 35 ◽  
Author(s):  
Elijah H. Kamau ◽  
Charlotte A. Serrem ◽  
Florence W. Wamunga
Keyword(s):  
Nutritional Quality ◽  
Protein Quality ◽  
Protein Energy Malnutrition ◽  
Milk Powder ◽  
Protein Digestibility ◽  
Albino Rats ◽  
Complementary Foods ◽  
Protein Energy ◽  
True Protein ◽  
Protein Free Diet

Compositing complementary flours with legumes of high protein quality such as soy can be effective in abating Protein Energy Malnutrition. Soy-fortified complementary flours were developed using locally available foods from Western Kenya and used to determine the fortification effect on protein nutritional quality, growth, and rehabilitation. Ten isonitrogenous diets containing 10% protein and one containing 20% protein were formulated from six foods; maize, pearl millet, finger millet, sorghum, cassava, and banana at ratios of 70:30 flour and soy with milk powder as control and fed to weanling male albino rats. Another group was fed on a protein free diet. Banana: Soy diet had significantly superior protein nutritional quality while Maize: soy had 70% Protein Digestibility Corrected Amino Acid Score. True Protein Digestibility of the fortified diets lay within the acceptable margin for cereal: bean mixtures. Fortification with soy improved protein quality indices of flours in rats, and by extrapolation could support growth of young children if used as complementary foods. 

scholarly journals Protein intakes in India

2012 ◽  
Vol 108 (S2) ◽  
pp. S50-S58 ◽  
Author(s):  
Sumathi Swaminathan ◽  
Mario Vaz ◽  
Anura V. Kurpad
Keyword(s):  
Amino Acid ◽  
Protein Intake ◽  
Fruits And Vegetables ◽  
Protein Quality ◽  
Protein Digestibility ◽  
Dietary Quality ◽  
Protein Energy ◽  
Amino Acid Score ◽  
Pe Ratio

Indian diets derive almost 60 % of their protein from cereals with relatively low digestibility and quality. There have been several surveys of diets and protein intakes in India by the National Nutrition Monitoring Board (NNMB) over the last 25 years, in urban and rural, as well as in slum dwellers and tribal populations. Data of disadvantaged populations from slums, tribals and sedentary rural Indian populations show that the protein intake (mainly from cereals) is about 1 gm/kg/day. However, the protein intake looks less promising in terms of the protein digestibility corrected amino acid score (PDCAAS), using lysine as the first limiting amino acid, where all populations, particularly rural and tribal, appear to have an inadequate quality to their protein intake. The protein: energy (PE) ratio is a measure of dietary quality, and has been used in the 2007 WHO/FAO/UNU report to define reference requirement values with which the adequacy of diets can be evaluated in terms of a protein quality corrected PE ratio. It is likely that about one third of this sedentary rural population is at risk of not meeting their requirements. These levels of risk of deficiency are in a population with relatively low BMI populations, whose diets are also inadequate in fruits and vegetables. Therefore, while the burden of enhancing the quality of protein intake in rural India exists, the quality of the diet, in general, represents a challenge that must be met.

scholarly journals Brief Research Report: Estimation of the Protein Digestibility-Corrected Amino Acid Score of Defatted Walnuts

2021 ◽  
Vol 8 ◽  
Author(s):  
Kimberly A. Lackey ◽  
Stephen A. Fleming
Keyword(s):  
Amino Acid ◽  
Protein Quality ◽  
Essential Fatty Acids ◽  
Protein Digestibility ◽  
Research Report ◽  
Tree Nuts ◽  
Amino Acid Score ◽  
True Protein ◽  
True Protein Digestibility

Introduction: Walnuts are considered a good source of essential fatty acids, which is unique among tree nuts. Walnuts are also composed of about 10–15% protein, but the quality of this protein has not been evaluated. Pistachios and almonds have been evaluated for their protein content using a protein digestibility-corrected amino acid score (PDCAAS), but it is unclear how the quality of protein in walnuts relates to that in other commonly consumed tree nuts. The objective of this study was to substantiate the protein quality of walnuts by determining their PDCAAS.Methods: A small, 10-day dietary intervention trial was conducted using male Sprague-Dawley rats (n = 8, 4 per group) with two diets: a nitrogen-free diet and a diet containing protein exclusively from defatted walnuts. Feed intake and fecal output of nitrogen were measured to estimate the true protein digestibility, and the amino acid compositions of walnuts compared to child and adult populations were used to calculate amino acid scores (AAS) and PDCAAS.Results: The true protein digestibility score of raw walnuts was calculated to be 86.22%. Raw walnuts contained 15.6 g protein/g walnut with AAS of 0.45 and 0.63 for children aged 6 months to 3 years and 3–10 years, respectively. For each population, a PDCAAS of 39 and 46% was calculated, respectively, using a protein conversion constant of 5.30. Using a protein constant of 6.25, a PDCAAS of 39% (6 months - 3 years) or 46% (3-10 years) was calculated.Conclusions: This is the first known assessment of the PDCAAS of walnuts. Like almonds, they appear to have a low-to-moderate score, indicating they are not a quality source of protein.

scholarly journals Making Nutritious Gluten-Free Foods from Quinoa Seeds and Its Flours

Proceedings ◽  
2020 ◽  
Vol 53 (1) ◽  
pp. 5
Author(s):  
Patricia Miranda Villa ◽  
Natalia Cervilla ◽  
Romina Mufari ◽  
Antonella Bergesse ◽  
Edgardo Calandri
Keyword(s):  
Celiac Disease ◽  
Amino Acid ◽  
Nutritional Quality ◽  
Protein Digestibility ◽  
World Population ◽  
Gluten Free ◽  
Treatment Needs ◽  
High Quality ◽  
The World ◽  
Amino Acid Score

Celiac disease is affecting around 1% of the world population and an effective treatment needs to exclude gluten. Quinoa is a high-quality gluten-free protein, and starch-rich endosperm, like the cereals. Protein contents and theoretical Protein Digestibility Corrected Amino Acid Score (PDCAAS) were evaluated in quinoas from Northwest and Centre of Argentina. A batter-type gluten-free quinoa bread was developed, showing good volume, taste, nutritional quality and a good long-lasting texture. Malted quinoa seeds’ quality indicators rose until 48 h of germination; after that, an unpleasant taste was developed. Muffins made with that flour showed acceptable taste.

scholarly journals Background on international activities on protein quality assessment of foods

2012 ◽  
Vol 108 (S2) ◽  
pp. S168-S182 ◽  
Author(s):  
G. Sarwar Gilani
Keyword(s):  
Amino Acid ◽  
Quality Assessment ◽  
Working Group ◽  
Protein Quality ◽  
Protein Digestibility ◽  
Expert Consultation ◽  
Codex Committee ◽  
Vegetable Proteins ◽  
Amino Acid Score

The subject of protein quality assessment of foods and diets was addressed at the Codex Committee on Vegetable Proteins (1982–1989), FAO/WHO (1989, 2001) and WHO/FAO (2002) expert reviews. These international developments are summarized in this manuscript. In 1989, a Joint FAO/WHO Expert Consultation on Protein Quality Evaluation reviewed knowledge of protein quality assessment of foods, and specifically evaluated amino acid score corrected for protein digestibility, the method recommended by the Codex Committee on Vegetable Proteins. The report of the Consultation published in 1991 concluded that the Protein Digestibility-corrected Amino Acid Score (PDCAAS) method was the most suitable approach for routine evaluation of protein quality for humans. The Consultation recognized that the amino acid scoring pattern proposed by FAO/WHO/UNU (1985) for preschool children was at that time the most suitable pattern for calculating PDCAAS for all ages except infants in which case the amino acid composition of human milk was recommended to be the basis of the scoring pattern. The rat balance method was considered as the most suitable practical method for predicting protein digestibility by humans. Since its adoption by FAO/WHO (1991), the PDCAAS method has been criticised for a number of reasons. The FAO/WHO (2001) Working Group on analytical issues related to protein quality assessed the validity of criticisms of the PDCAAS method. While recognizing a distinct regulatory use of protein quality data, the Working Group recommended that the PDCAAS method may be inappropriate for the routine prediction of protein quality of novel and sole source foods which contain high levels of anti nutritional factors; and that for regulatory purposes, the method should be revised to permit values of >100 for high quality proteins. In evaluating the recommendations of the Working Group, the WHO/FAO (2002) Expert Consultation on Protein and Amino Acid Requirements endorsed the PDCAAS method with minor modifications to the calculation method but also raised several issues. These included the calculation of scoring patterns; prediction of amino acid digestibility by faecal and ileal methods; reduced bioavailability of lysine in processed proteins; truncation of the amino acid score and consequent PDCAAS value; protein digestibility as a first limiting factor in determining the overall available dietary nitrogen; and the calculation of amino acid score for a dietary protein mixture. These concerns were considered particularly important in relation to the regulatory aspects of protein quality of foods, and their resolution was urgently recommended through a new separate expert review.

Evaluation Of Protein Digestibility-Corrected Amino Acid Score Method For Assessing Protein Quality Of Foods

1990 ◽  
Vol 73 (3) ◽  
pp. 347-356 ◽  
Author(s):  
Ghulam arwar ◽  
Frank E Mcdonough
Keyword(s):  
Amino Acid ◽  
Ad Hoc ◽  
Protein Quality ◽  
Quality Measurement ◽  
Protein Digestibility ◽  
Detailed Examination ◽  
True Digestibility ◽  
Score Method ◽  
Amino Acid Score

Abstract The current concepts of protein quality evaluation were reviewed. A detailed examination of existing animal assays and more promising amino acid scoring methods has been carried out by an Ad Hoc Working Group on Protein Quality Measurement for the Codex Committee on Vegetable Proteins during the last 5 years. Several factors such as Inadequacies of protein efficiency ratio (PER, the poorest test) and other animal assays, advancements made In standardizing methods for amino acid analysis and protein digestibility, availability of data on digestibility of protein and Individual amino acids in a variety of foods, and reliability of human amino acid requirements and scoring patterns were evaluated. On the basis of this evaluation, amino acid score, corrected for true digestibility of protein, was recommended to be the most suitable routine method for predicting protein quality of foods for humans. Amino acid scores corrected for true digestibility of protein (as determined by rat balance method) were termed "protein digestibility-corrected amino acid scores." A detailed method for the determination of the protein digestibility-corrected amino acid score was proposed, and information about the range of scores to be expected In foods or food products was provided In the present Investigation. The protein digestibility-corrected amino acid score method Is a simple and scientifically sound approach for routine evaluation of protein quality of foods. Accuracy of the method would, however, be confirmed after validation with growth or metabolic balance studies In humans.

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