scholarly journals Chromatographic Monoliths for High-Throughput Immunoaffinity Isolation of Transferrin from Human Plasma

2016 ◽  
Vol 89 (2) ◽  
Author(s):  
Irena Trbojević-Akmačić ◽  
Blaž Nemec ◽  
Urška Vidic ◽  
Suzana Malić ◽  
Karmela Miklić ◽  
...  
2021 ◽  
Vol 2 (3) ◽  
pp. 100606
Author(s):  
Giuseppina E. Grieco ◽  
Guido Sebastiani ◽  
Daniela Fignani ◽  
Noemi Brusco ◽  
Laura Nigi ◽  
...  

2009 ◽  
Vol 877 (27) ◽  
pp. 3159-3168 ◽  
Author(s):  
Isabela Tarcomnicu ◽  
Mihaela Cristina Gheorghe ◽  
Luigi Silvestro ◽  
Simona Rizea Savu ◽  
Irina Boaru ◽  
...  
Keyword(s):  

2019 ◽  
Vol 91 (4) ◽  
pp. 3125-3130 ◽  
Author(s):  
Tomohiro Yamada ◽  
Taro Saito ◽  
Yoshia Hill ◽  
Yutaka Shimizu ◽  
Kaori Tsukakoshi ◽  
...  

1989 ◽  
Vol 35 (10) ◽  
pp. 2112-2115 ◽  
Author(s):  
N S Gerasimova ◽  
I V Steklova ◽  
T Tuuminen

Abstract We adapted the method of McCaman and Robins for fluorometry of phenylalanine to a microplate assay for routine phenylketonuria screening. Sensitivity is 15 mumol/L for the plasma assay and 30 mumol/L for the dried blood-spot assay, with CV less than 10% for both assays. Results for human plasma by microplate assay correlated well (r = 0.99) with results of amino acid analyzer determination of phenylalanine. When measurements are performed in an automated reader, the microplate assay has considerable advantages over conventional measurements in cuvettes: smaller volumes of reagents and automation enabling high throughput and convenience. Because the described method is a quantitative one, we can postulate that, compared with the semiquantitative Guthrie inhibition bioassay, this microplate assay is more reliable, easier to perform, and about twofold less costly.


2005 ◽  
Vol 37 (5) ◽  
pp. 1039-1048
Author(s):  
Tapan K. Majumdar ◽  
Ray Bakhtiar ◽  
Cindy Chen ◽  
Luis Ramos ◽  
Francis L.S. Tse

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