Mycobacterium tuberculosis genotypic drug resistance patterns and clustering in Jayapura, Papua, Indonesia

2015 ◽  
Vol 19 (4) ◽  
pp. 428-433 ◽  
Author(s):  
L. Chaidir ◽  
S. Sengstake ◽  
J. de Beer ◽  
H. Krismawati ◽  
F. D. Lestari ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Resistance Patterns

scholarly journals Genomic Variations in Drug Resistant Mycobacterium tuberculosis Strains Collected from Patients with Different Localization of Infection

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 27
Author(s):  
Ekaterina Chernyaeva ◽  
Mikhail Rotkevich ◽  
Ksenia Krasheninnikova ◽  
Alla Lapidus ◽  
Dmitrii E. Polev ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Central Asia ◽  
Phylogenetic Analyses ◽  
Treatment Success ◽  
Point Mutations ◽  
Whole Genome Sequence ◽  
Public Health Importance ◽  
Resistance Patterns

Mycobacterium tuberculosis is a highly studied pathogen due to public health importance. Despite this, problems like early drug resistance, diagnostics and treatment success prediction are still not fully resolved. Here, we analyze the incidence of point mutations widely used for drug resistance detection in laboratory practice and conduct comparative analysis of whole-genome sequence (WGS) for clinical M. tuberculosis strains collected from patients with pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (XPTB) localization. A total of 72 pulmonary and 73 extrapulmonary microbiologically characterized M. tuberculosis isolates were collected from patients from 2007 to 2014 in Russia. Genomic DNA was used for WGS and obtained data allowed identifying major mutations known to be associated with drug resistance to first-line and second-line antituberculous drugs. In some cases previously described mutations were not identified. Using genome-based phylogenetic analysis we identified M. tuberculosis substrains associated with distinctions in the occurrence in PTB vs. XPTB cases. Phylogenetic analyses did reveal M. tuberculosis genetic substrains associated with TB localization. XPTB was associated with Beijing sublineages Central Asia (Beijing CAO), Central Asia Clade A (Beijing A) and 4.8 groups, while PTB localization was associated with group LAM (4.3). Further, the XPTB strain in some cases showed elevated drug resistance patterns relative to PTB isolates. HIV was significantly associated with the development of XPTB in the Beijing B0/W148 group and among unclustered Beijing isolates.

scholarly journals Role of embB Codon 306 Mutations in Mycobacterium tuberculosis Revisited: a Novel Association with Broad Drug Resistance and IS6110 Clustering Rather than Ethambutol Resistance

2005 ◽  
Vol 49 (9) ◽  
pp. 3794-3802 ◽  
Author(s):  
Manzour Hernando Hazbón ◽  
Miriam Bobadilla del Valle ◽  
Marta Inírida Guerrero ◽  
Mandira Varma-Basil ◽  
Ingrid Filliol ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Odds Ratio ◽  
Univariate Analysis ◽  
Drug Susceptibility ◽  
Drug Resistant ◽  
Development Of Resistance ◽  
Resistance Patterns ◽  
Novel Association

ABSTRACT Mutations at position 306 of embB (embB306) have been proposed as a marker for ethambutol resistance in Mycobacterium tuberculosis; however, recent reports of embB306 mutations in ethambutol-susceptible isolates caused us to question the biological role of this mutation. We tested 1,020 clinical M. tuberculosis isolates with different drug susceptibility patterns and of different geographical origins for associations between embB306 mutations, drug resistance patterns, and major genetic group. One hundred isolates (10%) contained a mutation in embB306; however, only 55 of these mutants were ethambutol resistant. Mutations in embB306 could not be uniquely associated with any particular type of drug resistance and were found in all three major genetic groups. A striking association was observed between these mutations and resistance to any drug (P < 0.001), and the association between embB306 mutations and resistance to increasing numbers of drugs was highly significant (P < 0.001 for trend). We examined the association between embB306 mutations and IS6110 clustering (as a proxy for transmission) among all drug-resistant isolates. Mutations in embB306 were significantly associated with clustering by univariate analysis (odds ratio, 2.44; P = 0.004). In a multivariate model that also included mutations in katG315, katG463, gyrA95, and kasA269, only mutations in embB306 (odds ratio, 2.14; P = 0.008) and katG315 (odds ratio, 1.99; P = 0.015) were found to be independently associated with clustering. In conclusion, embB306 mutations do not cause classical ethambutol resistance but may predispose M. tuberculosis isolates to the development of resistance to increasing numbers of antibiotics and may increase the ability of drug-resistant isolates to be transmitted between subjects.

scholarly journals Primary and acquired drug resistance patterns of Mycobacterium tuberculosis isolates in India: A multicenter study

2013 ◽  
Vol 6 (6) ◽  
pp. 456-464 ◽  
Author(s):  
Jitendra Prasad Mathuria ◽  
Jai Kumar Samaria ◽  
Govind Narayan Srivastava ◽  
Bharat Lal Mathuria ◽  
Sanjay Kumar Ojha ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Multicenter Study ◽  
Acquired Drug Resistance ◽  
Resistance Patterns

scholarly journals Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains observed in a tuberculosis high-burden municipality in Northeast, Brazil

2013 ◽  
Vol 17 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Roberta dos Santos Silva Luiz ◽  
Phillip Suffys ◽  
Elizabeth Clara Barroso ◽  
Ligia Regina Franco Sansigolo Kerr ◽  
Cynthia Romariz Duarte ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Northeast Brazil ◽  
High Burden ◽  
Resistance Patterns

scholarly journals Epidemiological Analysis of Mycobacterium tuberculosis Strains Isolated from Patients of Small Communities Living in the South-East of Poland

2015 ◽  
Vol 64 (3) ◽  
pp. 289-293
Author(s):  
Anna Zaczek ◽  
Iwona Szwaja ◽  
Monika Skiba ◽  
Anna Brzostek ◽  
Czeslaw Puchalski ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Population Size ◽  
Hybridization Technique ◽  
Great Chance ◽  
Epidemiological Analysis ◽  
Small Communities ◽  
Resistance Patterns ◽  
Dna Profiles ◽  
Pcr Techniques

The diversity of Mycobacterium tuberculosis clinical isolates, collected from a single hospital, was analyzed by ligation-mediated PCR techniques: FLiP and FLAP, and hybridization technique, IS6110-RFLP. The isolated strains were divided in terms of location (3 towns of Podkarpackie voivodeship differing in population size) and relationship (8 members of 4 families, each represented by 2 patients). Within each family identical DNA profiles, as well as drug resistance patterns were identified indicating a great chance of transmission of strains within the same family. Identical, or very similar patterns were also shared by strains isolated from unrelated patients living in a very small town (1 200 inhabitants) or hospitalized in the same place and time.

scholarly journals Evolution of Drug Resistance in Different Sublineages of Mycobacterium tuberculosis Beijing Genotype

2006 ◽  
Vol 50 (8) ◽  
pp. 2820-2823 ◽  
Author(s):  
Igor Mokrousov ◽  
Wei Wei Jiao ◽  
Gui Zhi Sun ◽  
Jia Wen Liu ◽  
Violeta Valcheva ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Population Structure ◽  
Selective Pressure ◽  
Beijing Genotype ◽  
Beijing Family ◽  
Antituberculosis Drugs ◽  
Beijing Area ◽  
Resistance Patterns ◽  
Mechanisms Of Adaptation

ABSTRACT We compared the population structure and drug resistance patterns of the Mycobacterium tuberculosis strains currently circulating in the Beijing area of China. One hundred thirteen of 123 strains belonged to the Beijing family genotypes defined by spoligotyping. The Beijing genotype strains were further subdivided into old and modern sublineages on the basis of NTF locus analysis. A stronger association with resistance to the more recently introduced antituberculosis drugs has been observed for old versus modern strains of the Beijing genotype, suggesting that its different sublineages may differ in their mechanisms of adaptation to drug selective pressure.

scholarly journals Signatures of Selection at Drug Resistance Loci in Mycobacterium tuberculosis

mSystems ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Tatum D. Mortimer ◽  
Alexandra M. Weber ◽  
Caitlin S. Pepperell
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Natural Populations ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Tb Treatment ◽  
Significant Burden ◽  
Resistance Patterns ◽  
Signatures Of Selection ◽  
Selection For

Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), is a significant burden on global health. Antibiotic treatment imposes strong selective pressure on M. tuberculosis populations. Identifying the mutations that cause drug resistance in M. tuberculosis is important for guiding TB treatment and halting the spread of drug resistance. Whole-genome sequencing (WGS) of M. tuberculosis isolates can be used to identify novel mutations mediating drug resistance and to predict resistance patterns faster than traditional methods of drug susceptibility testing. We have used WGS from natural populations of drug-resistant M. tuberculosis to characterize effects of selection for advantageous mutations on patterns of diversity at genes involved in drug resistance. The methods developed here can be used to identify novel advantageous mutations, including new resistance loci, in M. tuberculosis and other clonal pathogens.

scholarly journals DNA fingerprinting and drug resistance patterns of active pulmonary Mycobacterium tuberculosis in Mansoura hospitals, Egypt

2014 ◽  
Vol 63 (2) ◽  
pp. 369-375 ◽  
Author(s):  
Amina M. Abd-El Aal ◽  
Salah A. Agha ◽  
Mohamed Hosam E. Zaghloul ◽  
Heba A. Elshahawy ◽  
Dalia M. Abdel Azim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Dna Fingerprinting ◽  
Resistance Patterns

scholarly journals GENETIC FACTORS ASSOCIATED WITH MUTATIONS OF MOLECULAR MECHANISM AND DRUG RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS

2021 ◽  
Vol 2021 (1) ◽  
Author(s):  
F Arif ◽  
T Tahir ◽  
S Suhail ◽  
R Anees ◽  
I Nadeem ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Genetic Factors ◽  
Resistance Mechanism ◽  
Mortality Rates ◽  
Factors Associated ◽  
High Prevalence ◽  
Resistance Patterns ◽  
Mdr Tb ◽  
Chromosomal Mutations

Mycobacterium tuberculosis shows drug resistance patterns (drug-resistance tuberculosis DR-TB) for strains that are induced with high mortality rates. Because this acid-fast bacterium resists extensively against drugs and masks their effects to control the disease. However, these chromosomal mutations and genetic factors lead towards recent anti-TB drug discoveries. Anti-TB regimens are dearth to control this pandemic problem due to the high prevalence of this disease. These situations are remarkably given new ray to discover newer drugs that target such bacilli strains genetic factors and mutations. Also, it provides molecular updates to the resistance mechanism of mutations and genetic factors as a basic target then screened-out recent new anti-TB agents to limit the MDR-TB.

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