SMPTE Technical Note: Three-Dimensional Reconstruction: A Case Study of a Perspective Problem

This chapter reviews methods and techniques of digital reconstruction of disappeared architectural masterpieces. Through the overview of theoretical positions are explored heuristic values and communicative potentials of three-dimensional reconstruction, and prerogatives of animation in the interaction with three-dimensional data. Different critical approaches related to the architectures remained on paper are then brought to light. The examination of several case studies, also personally conducted, is extended to unbuilt architectures and minor architectures subject to demolition or transformation in order to highlight the different strategies used for the preservation of the memory of such heritage. Finally, the case study of Turin Horse-Racing Society Building by Carlo Mollino is extensively presented through the analysis of contemporary critical bibliography concerning the project, a description of archival sources and illustration of methods and techniques of the digital reconstruction.

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Digital Reconstruction of Demolished Architectural Masterpieces, 3D Modeling, and Animation

Advances in Geospatial Technologies - Handbook of Research on Emerging Digital Tools for Architectural Surveying, Modeling, and Representation ◽

10.4018/978-1-4666-8379-2.ch017 ◽

2015 ◽

pp. 476-509 ◽

Cited By ~ 7

Author(s):

Roberta Spallone

Keyword(s):

Case Studies ◽

3D Modeling ◽

Three Dimensional ◽

Horse Racing ◽

Three Dimensional Reconstruction ◽

Digital Reconstruction ◽

Methods And Techniques ◽

Dimensional Reconstruction ◽

Critical Approaches

This chapter reviews methods and techniques of digital reconstruction of disappeared architectural masterpieces. Through the overview of theoretical positions are explored heuristic values and communicative potentials of three-dimensional reconstruction, and prerogatives of animation in the interaction with three-dimensional data. Different critical approaches related to the architectures remained on paper are then brought to light. The examination of several case studies, also personally conducted, is extended to unbuilt architectures and minor architectures subject to demolition or transformation in order to highlight the different strategies used for the preservation of the memory of such heritage. Finally, the case study of Turin Horse-Racing Society Building by Carlo Mollino is extensively presented through the analysis of contemporary critical bibliography concerning the project, a description of archival sources and illustration of methods and techniques of the digital reconstruction.

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Three-dimensional reconstruction on cell level: case study elucidates the ultrastructure of the spinning apparatus of Embia sp. (Insecta: Embioptera)

Royal Society Open Science ◽

10.1098/rsos.160563 ◽

2016 ◽

Vol 3 (10) ◽

pp. 160563 ◽

Cited By ~ 4

Author(s):

Sebastian Büsse ◽

Thomas Hörnschemeyer ◽

Christian Fischer

Keyword(s):

High Resolution ◽

Three Dimensional ◽

Silk Gland ◽

Cellular Level ◽

Ultrastructural Organization ◽

Three Dimensional Reconstruction ◽

Dimensional Reconstruction ◽

Block Face ◽

Morphological Research

Spinning is a phenomenon not only present in spiders, but also in many other arthropods. The functional morphology and complexity of spinning organs is often poorly understood. Their elements are minute and studying them poses substantial methodological difficulties. This study presents a three-dimensional reconstruction of a silk gland of Embia sp. on cellular level, based on serial sections acquired with serial block-face scanning electron microscopy (SBFSEM) to showcase the power of this method. Previous studies achieved either high resolution to elucidate the ultrastructure or satisfying three-dimensional representations. The high-resolution achieved by SBFSEM can be easily used to reconstruct the three-dimensional ultrastructural organization of cellular structures. The herein investigated spinning apparatus of Embioptera can be taken as an example demonstrating the potential of this method. It was possible to reconstruct a multinucleated silk gland containing 63 nuclei. We focused on the applicability of this method in the field of morphological research and provide a step-by-step guide to the methodology. This will help in applying the method to other arthropod taxa and will help significantly in adapting the method to other animals, animal parts and tissues.

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Methods for three-dimensional reconstruction of cellular components within a thick section

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141871 ◽

1986 ◽

Vol 44 ◽

pp. 18-21

Author(s):

J. Frank ◽

B. F. McEwen ◽

M. Radermacher ◽

C. L. Rieder

Keyword(s):

Tilt Angle ◽

Tomographic Reconstruction ◽

3D Structure ◽

Three Dimensional ◽

Thick Section ◽

Three Dimensional Reconstruction ◽

Axis Ratio ◽

Dimensional Reconstruction ◽

Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

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Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100181014 ◽

1990 ◽

Vol 48 (1) ◽

pp. 452-453

Author(s):

Nicolas Boisset ◽

Jean-Christophe Taveau ◽

Jean Lamy ◽

Terence Wagenknecht ◽

Michael Radermacher ◽

...

Keyword(s):

Electron Microscopy ◽

Solid Body ◽

Body Surface ◽

Three Dimensional ◽

Staining Technique ◽

Three Dimensional Reconstruction ◽

Dimensional Reconstruction ◽

View Reconstruction ◽

Surface Representations ◽

Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

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Three-Dimensional Reconstruction of Two Forms of the Chaperonin GRO EL

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100180045 ◽

1990 ◽

Vol 48 (1) ◽

pp. 258-259

Author(s):

J.L. Carrascosa ◽

G. Abella ◽

S. Marco ◽

M. Muyal ◽

J.M. Carazo

Keyword(s):

Three Dimensional ◽

The Other ◽

Two Dimensional ◽

Series Method ◽

Three Dimensional Reconstruction ◽

Structural Components ◽

E Coli ◽

Dimensional Reconstruction ◽

Morphogenetic Factors ◽

Tilt Series

Chaperonins are a class of proteins characterized by their role as morphogenetic factors. They trantsiently interact with the structural components of certain biological aggregates (viruses, enzymes etc), promoting their correct folding, assembly and, eventually transport. The groEL factor from E. coli is a conspicuous member of the chaperonins, as it promotes the assembly and morphogenesis of bacterial oligomers and/viral structures.We have studied groEL-like factors from two different bacteria:E. coli and B.subtilis. These factors share common morphological features , showing two different views: one is 6-fold, while the other shows 7 morphological units. There is also a correlation between the presence of a dominant 6-fold view and the fact of both bacteria been grown at low temperature (32°C), while the 7-fold is the main view at higher temperatures (42°C). As the two-dimensional projections of groEL were difficult to interprete, we studied their three-dimensional reconstruction by the random conical tilt series method from negatively stained particles.

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