scholarly journals Potential applications of plant in vitro cultures in phytoremediation studies

2017 ◽  
Vol 8 (2) ◽  
pp. 11-17
Author(s):  
Marta Jaskulak ◽  
Anna Grobelak
Keyword(s):  
Plant Cell ◽  
In Vitro Cultures ◽  
Environmental Stressors ◽  
Tissue Cultures ◽  
Organ Cultures ◽  
Advantages And Disadvantages ◽  
Potential Applications ◽  
Plant Tissue Cultures ◽  
Induced Proteins

The main aim of this review is to assess the advantages and disadvantages of use of in vitro plant cell and organ cultures as useful research tools in process of phytoremediation. Plant tissue cultures including cell suspensions, callus and hairy roots are frequently used in the phytoremediation research, mostly as a model plant systems. One of the most important advantages of using in vitro cultures is the ability to examine the metabolic capabilities of plant cells as well as their capacity for toxicity tolerance in controlled conditions without any interference from microorganisms and processes occurring naturally in soils. The results obtained from plant cell or tissue cultures can be used to predict the responses of plants to environmental stressors and also to mass produce stress induced proteins and other metabolites. The aim of this review is to present possible applications for in vitro cultures in phytoremediation studies.

Inducing effect of plant cells on nitrogenase activity by Spirillum and Rhizobium in vitro

1978 ◽  
Vol 24 (2) ◽  
pp. 143-148 ◽  
Author(s):  
J. J. Child ◽  
W. G. W. Kurz
Keyword(s):  
Plant Cell ◽  
Callus Tissue ◽  
Nitrogenase Activity ◽  
Nutritional Requirement ◽  
Tissue Cultures ◽  
Cell Tissue ◽  
Direct Association ◽  
Pentose Sugar ◽  
Rhizobium Sp

Eleven different plant cell tissue cultures of both legume and non-legume origin have been grown in direct association, and in separate but close proximal association with both Spirillum lipoferum and Rhizobium sp. 32H1. Basic similarities were found in the nutritional requirement for the induction of nitrogenase activity (C2H2) in both organisms. In the absence of plant cell cultures both organisms need to be provided with a pentose sugar and a tricarboxylic acid to induce high levels of nitrogen-fixing activity. Plant cell callus tissue appears only capable of supplying the tricarboxylic acids needed but not the sugar component. The plant tissue, however, seems able to activate certain carbohydrates, which in themselves are incapable of substituting for the pentose additive.

Analysis of laser-induced fluorescence spectra of in vitro plant tissue cultures

2007 ◽  
Vol 46 (11) ◽  
pp. 2138 ◽  
Author(s):  
Ana Celia Muñoz-Muñoz ◽  
Humberto Gutiérrez-Pulido ◽  
José Manuel Rodríguez-Domínguez ◽  
Antonia Gutiérrez-Mora ◽  
Benjamín Rodríguez-Garay ◽  
...  
Keyword(s):  
Plant Tissue ◽  
Fluorescence Spectra ◽  
Laser Induced Fluorescence ◽  
Tissue Cultures ◽  
Plant Tissue Cultures ◽  
In Vitro Plant

Correlation of PCB Transformation by Plant Tissue Cultures with Their Morphology and Peroxidase Activity Changes

1999 ◽  
Vol 64 (9) ◽  
pp. 1497-1509 ◽  
Author(s):  
Petra Kučerová ◽  
Martina Macková ◽  
Ludmila Poláchová ◽  
Jiří Burkhard ◽  
Kateřina Demnerová ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Polychlorinated Biphenyls ◽  
Peroxidase Activity ◽  
Plant Tissue ◽  
Plant Cells ◽  
Tissue Cultures ◽  
Transforming Activity ◽  
Plant Tissue Cultures ◽  
Transformation Capacity

The ability of plant cells cultivated in vitro to metabolize polychlorinated biphenyls (PCBs) was correlated with the morphology of the cultures tested as models for phytoremediation studies. More differentiated cultures showed generally higher transformation capacity. The ability of plant cells to transform PCBs is connected to their viability in the presence of PCBs and their behaviour can be positively correlated with the production of intracellular and extracellular peroxidases. The cultures with high PCB-transforming activity proved to exhibit high peroxidase activity in the presence of PCBs while those with low ability to metabolize PCB showed a decrease of the enzyme activity in the presence of PCBs. Experiments with propylgallate were used to distinguish the ratio of involvement of peroxidases in PCB metabolism.

scholarly journals In vitro Degradation of 2,4,6-Trinitrotoluene Using Plant Tissue Cultures ofSolanum aviculare andRheum palmatum

2004 ◽  
Vol 4 (1) ◽  
pp. 46-49 ◽  
Author(s):  
A. Nepovím ◽  
M. Hubálek ◽  
R. Podlipná ◽  
S. Zeman ◽  
T. Vanek
Keyword(s):  
Plant Tissue ◽  
Tissue Cultures ◽  
In Vitro Degradation ◽  
Plant Tissue Cultures

scholarly journals Growth of Plant Tissue Cultures in Ultra-high Levels of Carbon Dioxide under Autotrophic and Heterotrophic Conditions

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 587e-587
Author(s):  
Brent Tisserat ◽  
Robert Silman
Keyword(s):  
Plant Tissue ◽  
Gas Permeability ◽  
Tissue Cultures ◽  
Natural Sunlight ◽  
Sterile Culture ◽  
Growth Modes ◽  
Plant Tissue Cultures ◽  
Culture Growth ◽  
Almost All

A comparative study was undertaken to determine the influence of lighting, carbohydrate concentrations and ultra-high levels of CO2, i.e., >10,000 ppm, on sterile culture growth. Past CO2-sterile studies have confirmed that elevation of CO2 to as high as 1000 ppm resulted in beneficial growth. Within special constructed chambers, tissue cultures were given a variety of CO2 levels for 12–16 hours/day using artificial lighting and natural sunlight. Several different plants (lettuce, beans, pine) and plant culture types were grown in CO2-enriched environments, ranging from 350 to 50,000 ppm. In almost all cases, plant tissue cultures not only tolerated but exhibited enhanced growth using ultra-high levels of CO2. For example, lettuce cultures were found to grow 2 to 4 times faster under ultra-high CO2. levels than under normal atmospheric CO2 levels, i.e., 350 ppm. Natural sunlight was found to be suitable for sterile culture growth. Modes of administration of CO2 in vitro and gas permeability of various culture vessels are presented.

scholarly journals Sekonder Bitki Metabolitlerinin In Vitro Koşullarda Üretimi

2019 ◽  
Vol 7 (7) ◽  
pp. 971
Author(s):  
Tuncay Çalışkan ◽  
Rüştü Hatipoğlu ◽  
Saliha Kırıcı
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Plant Cell ◽  
Abiotic Factors ◽  
Food Additives ◽  
Plant Secondary Metabolites ◽  
Secondary Metabolite Production ◽  
Organ Cultures ◽  
Metabolite Production

Plant secondary metabolites are a group of organic compounds produced by plants to interact with biotic and abiotic factors and for the establishment of defence mechanism. Secondary metabolites are classified based on their biosynthetic origin and chemical structure. They have been used as pharmaceutical, agrochemical, flavours, fragrances, colours and food additives. Secondary metabolites are traditionally produced from the native grown or field grown plants. However, this conventional approach has some disadvantages such as low yield, instability of secondary metabolite contents of the plants due to geographical, seasonal and environmental variations, need for land and heavy labour to grow plants. Therefore, plant cell and organ cultures have emerged as an alternative to plant growing under field conditions for secondary metabolite production. In this literature review, present state of secondary metabolite production through plant cell and organ cultures, its problems as well as solutions of the problems were discussed.

GROWTH OF LETTUCE AND CAULIFLOWER TISSUES IN VITRO AND THEIR PRODUCTION OF ANTIMICROBIAL METABOLITES

1965 ◽  
Vol 11 (5) ◽  
pp. 785-789 ◽  
Author(s):  
Gordon Campbell ◽  
E. C. S. Chan ◽  
W. G. Barker
Keyword(s):  
Insoluble Fraction ◽  
Plant Tissues ◽  
Naphthaleneacetic Acid ◽  
Tissue Cultures ◽  
Mycobacterium Phlei ◽  
Proliferative Growth ◽  
Plant Tissue Cultures ◽  
Antimicrobial Metabolites ◽  
Medical Interest

Plant tissue cultures of cauliflower and lettuce were successfully established on semisolid White"s medium supplemented with coconut milk (20%) and naphthaleneacetic acid (5 p.p.m.). On this medium the tissues exhibited vigorous proliferative growth with no tendency for organogenesis. Ethanol–ether extracts of the stale medium of these tissues yielded an alcohol-insoluble fraction that was highly and consistently inhibitory for Staphylococcus aureus but variable in antimicrobial activity against Mycobacterium phlei. The results suggest that in view of the progress in mass tissue culturing of plant cells in vitro, the use of cultured plant tissues should be explored for the production of antimicrobial principles of medical interest.

scholarly journals Biotransformation of a Monoterpene Mixture by in vitro Cultures of Selected Conifer Species

2007 ◽  
Vol 2 (3) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Marcela Dvořáková ◽  
Irena Valterová ◽  
Tomáš Vaněk
Keyword(s):  
Picea Abies ◽  
Suspension Culture ◽  
Organic Compounds ◽  
In Vitro Cultures ◽  
Taxus Baccata ◽  
Tissue Cultures ◽  
Conifer Species ◽  
Biotransformation Products ◽  
The Individual

Biotransformation of monoterpenes is a mild and promising method for the production of stereospecific and regiospecific organic compounds. It is advantageous, especially for the preparation of substances with complicated structures and for those that need to be classed as “natural products“. Our study has focused on the biotransformation of a monoterpenic mixture, turpentine, by Picea abies and Taxus baccata suspension cultures. We identified the biotransformation products and compared the metabolite compositions of the tissue cultures. The major biotransformation products of turpentine were trans-pinocarveol, trans-verbenol, verbenone, myrtenol, α-terpineol and trans-sobrerol, which correspond with the products of biotransformation of the individual monoterpenes, α-pinene and β-pinene, by P. abies suspension culture.

scholarly journals In vitro plant tissue cultures accumulate polyisoprenoid alcohols.

2007 ◽  
Vol 54 (4) ◽  
pp. 847-852 ◽  
Author(s):  
Karolina Skorupinska-Tudek ◽  
Anna Pytelewska ◽  
Monika Zelman-Femiak ◽  
Jakub Mikoszewski ◽  
Olga Olszowska ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Plant Tissue ◽  
Plant Cells ◽  
Ocimum Sanctum ◽  
Taxus Baccata ◽  
Root Cultures ◽  
Tissue Cultures ◽  
Cultivated Plant ◽  
Plant Tissue Cultures

In vitro cultivated plant cells and tissues were found to synthesize polyisoprenoids. Taxus baccata suspension cell cultures accumulated polyisoprenoids of the same pattern as the parental tissue; methyl jasmonate or chitosan treatment almost doubled their content. All the root cultures studied accumulated dolichols as predominant polyisoprenoids. Roots of Ocimum sanctum grown in vitro accumulated approx. 2.5-fold higher amount of dolichols than the roots of soil-grown plants. Dolichols dominated over polyprenols in all Triticum sp. tissues studied.

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