Comparison of mice’ sperm parameters exposed to some hazardous physical agents

The present study was aimed to compare the effects of exposure to noise, vibration, lighting, and microwave on male mice’ sperm parameters. The mice were randomly assigned to five groups of eight, which comprised of the unexposed group and exposure groups including the lighting (1000 lux), noise (100 dB(A)), vibration (acceleration of 1.2 m/s2) and microwave (power density of 5 watts). The exposure groups were subjected to the four agents for 8 hours a day, 5 days a week during a 2-week period. Semen analysis were done according to World Health Organization guidelines. The highest significant mean difference in sperm count (-1.35×106/mL) had being observed between the microwave group and the control one (P=0.001). The highest difference in immotile percent (25.88 %) had being observed between the noise group and the control one (P=0.001). The highest difference in normal morphology (-27.06 %) observed between the lighting exposure group and the control group (P=0.001). The four agents can cause changes in different sperm parameters, however for definite conclusion; more laboratory and field studies are required. In total, exposure to microwave has had the greatest effect on sperm count and exposure to light has had the greatest effect on normal morphology and non-progressive motility. Moreover, exposure to noise has had the greatest effect on progressive motility and immotile percent, respectively.

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From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

Journal of Clinical Medicine ◽

10.3390/jcm9020406 ◽

2020 ◽

Vol 9 (2) ◽

pp. 406 ◽

Cited By ~ 4

Author(s):

Rosita A. Condorelli ◽

Aldo E. Calogero ◽

Giorgio I. Russo ◽

Sandro La Vignera

Keyword(s):

Normal Form ◽

Sperm Motility ◽

Sperm Count ◽

Semen Analysis ◽

Sperm Concentration ◽

Sperm Parameters ◽

Sperm Parameter ◽

Progressive Motility ◽

Semen Volume ◽

Chromatin Compactness

The aim of this experimental study was to evaluate whether infertile patients may benefit from the evaluation of bio-functional sperm parameters in addition to the conventional semen analysis. To accomplish this, we evaluated the correlation between conventional and bio-functional sperm parameters based on their percentile distribution in search of a potential threshold of these latter that associates with conventional sperm parameter abnormalities. The study was conducted on 577 unselected patients with infertility lasting at least 12 months. We identified cut-off values according to the median of the population for mitochondrial membrane potential (MMP), number of alive spermatozoa, and chromatin abnormality. High MMP (HMMP) (≥46.25%) was associated with sperm concentration, sperm count, progressive motility, and normal form. Low MMP (LMMP) (≥36.5%) was found to be associated with semen volume, sperm concentration, total sperm count, progressive motility, total motility, and normal form. The number of alive spermatozoa (≥71.7%) was associated with sperm concentration and progressive motility whereas abnormal chromatin compactness (≥21.10%) was associated with sperm concentration, total sperm count, and progressive motility. The data would suggest that, for every increase in the percentile category of sperm concentration, the risk of finding an HMMP≤46.25 is reduced by 0.4 and by 0.66 for a total sperm count. This risk is also reduced by 0.60 for every increase in the percentile category of sperm progressive motility and by 0.71 for total sperm motility. Each increment of percentile category of the following sperm parameter was followed by a decrease in the risk of finding an LMMP≤36.5: sperm concentration 1.66, total sperm count 1.28, sperm progressive motility 1.27, total sperm motility 1.76, and normal form 1.73. Lastly, the data showed that, for every increase in the percentile category of total sperm count, the risk of finding an abnormal chromatin compactness ≤21.10 is reduced by 1.25 (1.04–1.51, p < 0.05) and an increase of total sperm motility is associated with a reduced risk by 1.44 (1.12–1.85, p < 0.05). Results suggest a correlation between bio-functional and conventional sperm parameters that impact the sperm fertilizing potential. Therefore, the evaluation of bio-functional sperm parameters by flow cytometry may be useful to explain some cases of idiopathic male infertility.

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Effect of Panax notoginseng Extracts on Inferior Sperm Motility in vitro

The American Journal of Chinese Medicine ◽

10.1142/s0192415x9900015x ◽

1999 ◽

Vol 27 (01) ◽

pp. 123-128 ◽

Cited By ~ 15

Author(s):

Jung-Chou Chen ◽

Ming-Xiong Xu ◽

Leih-Der Chen ◽

Yan-Nian Chen ◽

Tsan Hung Chiu

Keyword(s):

Sperm Motility ◽

Sperm Count ◽

Semen Analysis ◽

Panax Notoginseng ◽

World Health ◽

Computer Assisted ◽

Butanol Extract ◽

The World ◽

Health Organization

The purpose of this study was to investigate the effects of Panax notoginseng extracts on inferior sperm motility in vitro. Semen samples were collected from 23 patients with sperm motility between 20% and 40%. The sperm count was over 20 × 106/ml in accordance with the World Health Organization standard. 1.0 mg/ml and 2.0 mg/ml of Panax notoginseng extracts including aqueous extract, n-butanol extract, and polysaccharide fraction on sperm motility and progression were evaluated by computer assisted semen analysis. The results demonstrated that sperm motility as well as progression on inferior sperm motility were enhanced at 1 hour and 2 hours after incubation with all three types of extracts.

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SEMEN ANALYSIS; CORNER STONE IN EVALUATING INFERTILITY

Pakistan Armed Forces Medical Journal ◽

10.51253/pafmj.v71i6.6150 ◽

2022 ◽

Vol 71 (6) ◽

pp. 2224-27

Author(s):

Aysha Khan ◽

Zunera Shahbaz ◽

Shagufta Yousaf ◽

Abeera Ahmed ◽

Fatima Sana ◽

...

Keyword(s):

Sperm Count ◽

Semen Analysis ◽

Cross Sectional Study ◽

World Health ◽

Semen Parameters ◽

Military Hospital ◽

Normal Male ◽

Cross Sectional ◽

Normal Sperm ◽

Health Organization

Objective: To study the patterns and distribution of various abnormal semen parameters in infertile males. Study Design: Cross sectional study. Place and Duration of Study: Department of Pathology, Combined Military Hospital, Karachi, from Nov 2019 to Oct 2020. Methodology: The study included 364 patients who presented with primary and secondary infertility. Consecutive convenient sampling was done. Semen analysis was performed using World Health Organization latest guidelines. Samples were categorized as normospermia, azoospermia, oligospermia, asthenozoospermia and necrospermia. Results: The study comprised of 364 samples of infertile males. Normal sperm count was observed in 317 (87%) males, azoospermia in 28 (7.6%) and oligospermia in 19 (5.2%) males. Low ejaculated volume and higher non-motile sperms were noted in oligospermia samples in comparison with normospermia samples. Asthenozoospermia was observed in 102 (28%) and oligoasthenospermia was noted in 15 (4.1%) samples. Conclusion: Good quality semen analysis is a corner stone to diagnose the cause of male infertility.Sperm concentration and motility are the important markers of normal male reproductive system and are related to each other.

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AYURVEDIC MANAGEMENT OF KSHEENA SHUKRA (OLIGOSPERMIA) LEADING TO MALE INFERTILITY: A CASE REPORT

International Journal of Research in Ayurveda and Pharmacy ◽

10.7897/2277-4343.1204101 ◽

2021 ◽

Vol 12 (4) ◽

pp. 30-32

Author(s):

Akhilraj A R ◽

Amalraj A R

Keyword(s):

Case Report ◽

Male Infertility ◽

Conventional Medicine ◽

Sperm Count ◽

Semen Analysis ◽

World Health ◽

Case Discussion ◽

Ayurvedic Treatment ◽

Health Organization

As per the World Health Organization (WHO) report in India, the overall prevalence of primary infertility ranges between 3.9 to 16.8%. A recent report on status of infertility states that, 50% is related to reproductive anomalies or disorders in the male, in which 90% of male infertility problems are related to Oligospermia and other abnormalities in semen analysis. As per Ayurveda, Oligospermia can be considered as Ksheena Shukra. Surgery, Hormone treatments, Medications and Assisted Reproductive Technology (ART) are the main line of treatment in Conventional medicine, but these are associated with many unwanted and serious adverse effects. The present case discussion is about the effective management of a case diagnosed as Ksheena Shukra (Oligospermia) with Ayurvedic intervention, which was posted for ART and Hormone therapy. The aim of this case report is to evaluate the effect of Ayurvedic treatment in the management of Ksheena Shukra. In this case study, Shodhana procedures (especially Virechana karma) were performed before the administration of Rasayana and Vajikarana drugs, which are Narasimha rasayana, Bhringarajasavam, Ashwagandha churna. The pre and post Semen analysis on the treatment period shows, marked increase in sperm count and motility. The outcome of this case reveals that the Oligospermia associated with male infertility can be effectively managed by Ayurvedic treatments.

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Effects of Satureja Edmondi Briq on Ameliorate Reproductive Parameters in Pentylenetetrazol-Induced Epilepsy Models in Rats

10.21203/rs.3.rs-93397/v1 ◽

2020 ◽

Author(s):

somayeh bohlouli ◽

gelavij mahmoodi

Keyword(s):

Luteinizing Hormone ◽

Essential Oil ◽

Sperm Count ◽

Follicle Stimulating Hormone ◽

Reproductive Potential ◽

Sperm Parameters ◽

Male Rats ◽

World Health ◽

Health Organization ◽

Stimulating Hormone

Abstract BackgroundEpilepsy is one of the common neurological brain defects that causes unpredictable, recurrent seizures. It has adverse effects on the reproductive functions.Oxidative stress contributes to the evolution of epilepsy, including reduction of sperm count and motility,increase of abnormal sperm morphology, and low testosterone.Antioxidants are beneficial to spermatogenesis and sperm parameters. Satureja edmondiBriq is known as a powerful antioxidant that can reduce the effects of oxidative stress. The aim of this study was to evaluate the effects of Satureja edmondion reproductive potential in pentylenetetrazol (PTZ)-induced epileptic male rats.MethodsIn this experimental study, 48 Wistar male rats with an average age of 10 weeks and weightof 240- 260g were used. Animals were randomized into normal and kindled groups that were treated with different doses of S.edmondi essential oil. Treatment of animals lasted 4 weeks. In the end, testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm parameters were measured according to the World Health Organization (WHO) standards.ResultsThis study showed that all sperm parameters,including sperm count, sperm viability, and progressive sperm motility were increased and testosterone, follicle-stimulating hormone and luteinizing hormone (FSH,LH) changes in the epileptic and normal groups were changed with an increase in the dose of S.edmondi essential oil. ConclutionsThe changes were significant in some parameters. S.edmondi as a good source of antioxidants can improve sperm parameters and reproductive potential in PTZ-induced epileptic male rats.

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Use of Biosimilar Follicle-Stimulating Hormone in Asthenozoospermic Infertile Patients: A Multicentric Study

Journal of Clinical Medicine ◽

10.3390/jcm9051478 ◽

2020 ◽

Vol 9 (5) ◽

pp. 1478

Author(s):

Maurizio De Rocco Ponce ◽

Carlo Foresta ◽

Rocco Rago ◽

Alessandro Dal Lago ◽

Giancarlo Balercia ◽

...

Keyword(s):

Dna Fragmentation ◽

Sperm Count ◽

Semen Analysis ◽

Follicle Stimulating Hormone ◽

Sperm Concentration ◽

Comparable Efficacy ◽

Control Group ◽

Multicentric Study ◽

Progressive Motility ◽

Stimulating Hormone

There is increasing data in favour of follicle-stimulating hormone (FSH) therapy in patients with oligo-asthenozoospermia and normal-range gonadotropins in order to increase sperm count and above all sperm motility. Some studies showed an improvement in DNA fragmentation and spontaneous pregnancy. Recently, biosimilar FSH has been marketed with the same indications. We performed a retrospective multicentric case-control study involving 147 asthenozoospermic patients between 18 and 45 years of age. A total of 97 patients were treated with biosimilar FSH 150 UI three times a week for 3 months, while 50 control subjects received no treatment. Patients were evaluated at baseline and after 3 months with semen analysis including DNA fragmentation, testicular colour Doppler ultrasound, and blood tests. Spontaneous pregnancies were recorded during a further follow-up period of 6 months. Treated patients showed after treatment a statistically significant increase in sperm concentration, total sperm count, and total motile sperm, as well as improved progressive motility and non-progressive motility. DNA fragmentation showed a significant reduction. Conversely, in the control group, no significant change was found. Pregnancy rate was significantly higher in treated patients. These data suggest comparable efficacy of biosimilar FSH in the treatment of male infertility; however, larger studies are needed to confirm our results.

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Chronic treatment with CV 205-502 restores the gonadal function in hyperprolactinemic males

Acta Endocrinologica ◽

10.1530/eje.0.1350548 ◽

1996 ◽

Vol 135 (5) ◽

pp. 548-552 ◽

Cited By ~ 27

Author(s):

Annamaria Colao ◽

Michele De Rosa ◽

Francesca Sarnacchiaro ◽

Antonella Di Sarno ◽

Maria Luisa Landi ◽

...

Keyword(s):

Luteinizing Hormone ◽

Chronic Treatment ◽

Sperm Count ◽

Semen Analysis ◽

Follicle Stimulating Hormone ◽

Clinical Endocrinology ◽

Sperm Parameters ◽

Gonadal Function ◽

Good Tolerability ◽

Normal Morphology

Colao A, De Rosa M, Sarnacchiaro F, Di Sarno A, Landi ML, Iervolino E, Zarrilli S, Merola B, Lombardi G. Chronic treatment with CV 205-502 restores the gonadal function in hyperprolactinemic males. Eur J Endocrinol 1996;135:548–52. ISSN 0804–4643 The aim of this study was to evaluate the effects of a chronic treatment with the non-ergot-derived dopamine agonist quinagolide (CV 205-502) on sexual and gonadal function in hyperprolactinemic males. Thirteen males with macroprolactinoma and one with microprolactinoma were treated with CV 205-502 at the dose of 0.15–0.6 mg/day for 6–24 months. Baseline prolactin (PRL) was 464 ± 75.7 μg/l. All the patients suffered from libido impairment, five of reduced sexual potency, six had infertility and in four bilateral induced galactorrhea was shown. The semen analysis revealed a severe oligoasthenospermia with reduced sperm count, motility and forward progression, with an abnormal morphology and decreased viability. A significant reduction of serum PRL levels (nadir PRL = 12.3 ± 5.4 μg/l) was obtained during the treatment. Normalization of prolactinemia was reached in 13 of the 14 patients after 3 months. After 1 year, a significant improvement of sperm parameters, in terms of increase of number (from 5600 ± 111 to 20 564 ± 587 mm3), motility at 1 h (from 24.8 ± 0.1 to 52.6 ± 0.5%), forward progression (from 24 ± 1.4 to 62.3 ± 2.9%) and normal morphology (from 53.8 ± 2.5 to 62.2 ± 2.4%), was recorded. In addition, a significant increase of serum follicle-stimulating hormone (from 5.3 ± 0.6 to 7.8 ± 0.4 U/l), luteinizing hormone (from 4.4 ± 0.5 to 7.7 ± 0.4 U/l) and testosterone (from 3.4 ± 0.4 to 4.7 ± 0.2 μg/l) was recorded. A significant increase of luteinizing hormone (9.4 ± 0.7 U/l) and testosterone (5.2 ± 0.4 μg/l), as well as a further improvement of sperm parameters, was found after 2 years of therapy. Sellar computed tomography and/or magnetic resonance showed a considerable shrinkage (⩾ 30%) of tumoral mass in 8 out of 13 patients with macroprolacinoma. Side effects were recorded in only one patient. In conclusion, the treatment with CV 205-502 normalizing PRL levels improves gonadal and sexual function and fertility in males with prolactinoma, providing good tolerability and excellent patient compliance to medical treatment. This result demonstrates that the impairment of gonadal function in hyperprolactinemic patients is a functional modification. Annamaria Colao, Department of Molecular and Clinical Endocrinology and Oncology, "Federico II" University, via S. Pansini 5, 80131 Naples, Italy

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Evaluation of seminal fluid leukocyte subpopulations in patients with varicocele

International Journal of Immunopathology and Pharmacology ◽

10.1177/2058738420925719 ◽

2020 ◽

Vol 34 ◽

pp. 205873842092571

Author(s):

Laura Maria Mongioì ◽

Angela Alamo ◽

Aldo E Calogero ◽

Michele Compagnone ◽

Filippo Giacone ◽

...

Keyword(s):

Seminal Fluid ◽

Sperm Concentration ◽

Sperm Parameters ◽

World Health ◽

Lower Percentage ◽

Control Group ◽

Healthy Men ◽

Sperm Analysis ◽

The World ◽

Health Organization

Varicocele is a common cause of sperm damage. Some studies showed higher concentration of seminal leukocytes in patients with varicocele. The aim of the study was to evaluate seminal leukocyte subpopulations in patients with varicocele. We enrolled 20 patients with varicocele and 20 age-matched healthy men. Sperm analysis was conducted according to the World Health Organization (WHO) 2010 criteria. We evaluated seminal leukocyte subpopulations and bio-functional sperm parameters by flow cytometry. Patients with varicocele had significantly lower sperm concentration and total number than controls. Regarding seminal leukocyte subpopulations, patients with varicocele had a significantly lower percentage of CD8+ and CD16+ leukocytes and a significantly higher percentage of CD4+ leukocytes than controls. As for bio-functional sperm parameters, we found that patients with varicocele had a significantly lower percentage of alive spermatozoa compared to the control group. These results may explain the increased level of cytokines in the seminal plasma of patients with varicocele.

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Semen quality among Danish and Finnish men attempting to conceive. The Danish First Pregnancy Planner Study Team

Acta Endocrinologica ◽

10.1530/eje.0.1420047 ◽

2000 ◽

Vol 142 (1) ◽

pp. 47-52 ◽

Cited By ~ 36

Author(s):

TK Jensen ◽

M Vierula ◽

NH Hjollund ◽

M Saaranen ◽

T Scheike ◽

...

Keyword(s):

Semen Quality ◽

Sperm Count ◽

Semen Analysis ◽

Sperm Concentration ◽

Cohort Effect ◽

World Health ◽

Follow Up Studies ◽

Health Organization ◽

Selection Of

OBJECTIVE: To assess differences in semen quality between similar populations from Denmark and Finland. DESIGN: Comparison of semen quality between 221 Finnish men (of whom 115 had no proven fertility) and 411 Danish men with no proven fertility in two follow-up studies among normal couples trying to conceive. METHODS: In Finland male partners of couples without experienced infertility attempting to conceive were recruited through advertisements in local newspapers from 1984 to 1986. From 1992 to 1995 Danish men who lived with a partner and who had not attempted to achieve a pregnancy previously were recruited through their union when they discontinued birth control. All semen analyses were performed in accordance with the World Health Organization guidelines. RESULTS: Median sperm concentration, total sperm count and the percentage of morphologically normal spermatozoa were significantly higher among the Finnish men without proven fertility (104.0 million/ml, 304.0 million and 58% respectively) compared with the Danish men (53.0 million/ml, 140.8 million, and 41% respectively). Sperm concentration was 105.7% (95% confidence interval (CI) 58.1%-167.6%) and total sperm count was 127.4% (95% CI 71.4%-201.6%) higher among Finnish men without proven fertility than among Danish men after control for confounders. CONCLUSIONS: Some, but hardly all, of the observed difference in semen quality may be explained by differences in recruitment procedures, selection of the men and by methodological differences in semen analysis between the two countries. Also a birth cohort effect may explain some of the differences between countries as the Finnish men were recruited 11 years before the Danish men. Therefore, follow-up studies with identical recruitment and selection of men from the two countries are needed.

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The Effect of Varicocelectomy on Sperm Parameters in Subfertile Men with Clinical Varicoceles Who Have Asthenozoospermia or Teratozoospermia with Normal Sperm Density

ISRN Urology ◽

10.1155/2013/698351 ◽

2013 ◽

Vol 2013 ◽

pp. 1-3 ◽

Cited By ~ 3

Author(s):

Basri Cakiroglu ◽

Orhun Sinanoglu ◽

Ramazan Gozukucuk

Keyword(s):

Seminal Fluid ◽

Sperm Count ◽

Sperm Concentration ◽

Sperm Parameters ◽

World Health ◽

Sperm Density ◽

Normal Sperm ◽

The Mean ◽

Health Organization ◽

Fluid Parameters

Background. To compare preoperative and postoperative sperm parameters such as sperm count, motility, and morphology in patients with normal sperm concentration with teratozoospermia and asthenozoospermia. Materials and Methods. Hundred and six patients with varicocele associated with male infertility over a 5-year period were included into the study. Pre- and postvaricocelectomy seminal fluid parameters evaluation according to the World Health Organization (WHO) criteria was performed at 4–6-month intervals. Results. One hundred and six patients met the criteria. The mean age of patients was 24.53 ± 8.13. The mean duration of infertility was 3.6 years (range: 1.5–6.3). Only the sperm motility of patients with normospermia showed a significant improvement postoperatively. Conclusions. No significant improvement in sperm morphology may be obtained in patients with clinical varicocele and preoperative normospermia.

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