scholarly journals Evaluation of seminal fluid leukocyte subpopulations in patients with varicocele

2020 ◽  
Vol 34 ◽  
pp. 205873842092571
Author(s):  
Laura Maria Mongioì ◽  
Angela Alamo ◽  
Aldo E Calogero ◽  
Michele Compagnone ◽  
Filippo Giacone ◽  
...  
Keyword(s):  
Seminal Fluid ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
World Health ◽  
Lower Percentage ◽  
Control Group ◽  
Healthy Men ◽  
Sperm Analysis ◽  
The World ◽  
Health Organization

Varicocele is a common cause of sperm damage. Some studies showed higher concentration of seminal leukocytes in patients with varicocele. The aim of the study was to evaluate seminal leukocyte subpopulations in patients with varicocele. We enrolled 20 patients with varicocele and 20 age-matched healthy men. Sperm analysis was conducted according to the World Health Organization (WHO) 2010 criteria. We evaluated seminal leukocyte subpopulations and bio-functional sperm parameters by flow cytometry. Patients with varicocele had significantly lower sperm concentration and total number than controls. Regarding seminal leukocyte subpopulations, patients with varicocele had a significantly lower percentage of CD8+ and CD16+ leukocytes and a significantly higher percentage of CD4+ leukocytes than controls. As for bio-functional sperm parameters, we found that patients with varicocele had a significantly lower percentage of alive spermatozoa compared to the control group. These results may explain the increased level of cytokines in the seminal plasma of patients with varicocele.

scholarly journals The Effect of Varicocelectomy on Sperm Parameters in Subfertile Men with Clinical Varicoceles Who Have Asthenozoospermia or Teratozoospermia with Normal Sperm Density

ISRN Urology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Basri Cakiroglu ◽  
Orhun Sinanoglu ◽  
Ramazan Gozukucuk
Keyword(s):  
Seminal Fluid ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
World Health ◽  
Sperm Density ◽  
Normal Sperm ◽  
The Mean ◽  
Health Organization ◽  
Fluid Parameters

Background. To compare preoperative and postoperative sperm parameters such as sperm count, motility, and morphology in patients with normal sperm concentration with teratozoospermia and asthenozoospermia. Materials and Methods. Hundred and six patients with varicocele associated with male infertility over a 5-year period were included into the study. Pre- and postvaricocelectomy seminal fluid parameters evaluation according to the World Health Organization (WHO) criteria was performed at 4–6-month intervals. Results. One hundred and six patients met the criteria. The mean age of patients was 24.53 ± 8.13. The mean duration of infertility was 3.6 years (range: 1.5–6.3). Only the sperm motility of patients with normospermia showed a significant improvement postoperatively. Conclusions. No significant improvement in sperm morphology may be obtained in patients with clinical varicocele and preoperative normospermia.

scholarly journals Adherence to WHO 2010 Recommendations in Relation to Semen Analysis Reports in the Laboratories of West Bengal

2019 ◽  
Vol 01 (04) ◽  
pp. 187-192
Author(s):  
Sujoy Dasgupta
Keyword(s):  
Observational Study ◽  
Reference Values ◽  
West Bengal ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Reproductive Technology ◽  
World Health ◽  
The World ◽  
Health Organization ◽  
Better Than

Background: In 2010, The World Health Organization (WHO) suggested the standards of reporting of semen analysis and the reference values. We tried to determine the adherence to the WHO 2010 standard regarding semen analysis among the laboratories of West Bengal. Methods: An observational study was carried out by collecting the semen analysis reports from different laboratories. Compliance with the WHO 2010 recommendations regarding the reporting of semen analysis and references mentioned was subsequently analyzed. Results: A total of 211 laboratory reports were collected; of which 15 were ART (Assisted Reproductive Technology)-laboratories (7%) and 196 were non-ART-laboratories (93%). More than half of the laboratories did not mention any reference values. Only 7.5% used the phrase “WHO 2010” as the reference. Only 3% of the laboratories reported all the six “important” parameters (volume, pH, sperm concentration, motility, morphology and vitality) and used the WHO 2010 references for all of them. The ART laboratories performed significantly better than their non-ART counterparts in reporting and quoting the WHO 2010 reference values. Conclusion: Even nine years after its introduction, the compliance with the WHO 2010 recommendations on semen analysis was still low among our laboratories. There is need for increased awareness for the laboratory persons in this regard.

scholarly journals EFFECT OF GIVING CUCUMBER (Cucumis sativus) TO DECREASE HEMOGLOBIN TROMBOSITE AND IMPROVEMEN IN PRE-ECLAMMED PATIENTS AT DEWI SARTIKA HOSPITAL

2021 ◽  
Vol 3 (1) ◽  
pp. 1-7
Author(s):  
Novia Sarmiati ◽  
Erwin Azizi Jaya Dipraja ◽  
La Banudi
Keyword(s):  
Maternal Mortality ◽  
General Hospital ◽  
World Health ◽  
Group Method ◽  
Control Group ◽  
Live Births ◽  
The World ◽  
Quasi Experimental ◽  
Health Organization ◽  
Number Of Births

Background:According to the World Health Organization (WHO) every day in 2015, around 830 women die from complications of pregnancy and child birth. Globally, maternal mortality in the world is 303,000. The maternal mortality rate (MMR) in Indonesia based on the Indonesian Health Demographic Survey (IDHS) in 2012 was around 359 / 100,000 live births, this figure increased compared to 2007, which was around 228 / 100,000 live births. Data from Dewi Sartika General Hospital with preeclampsia cases for the last three years. In 2017, the number of births in 1855 who experienced preeclampsia were 305 cases. In 2018 the number of births in 1822 who experienced preeclampsia was 334 cases. In 2019 the number of deliveries in 2007 who experienced preeclampsia was 377 cases. For 2020 January to June number of deliveries695 the incidence of preeclampsia was 164 cases. Methods:This study used a quasi-experimental type with a pre-test-post-test control group method design. The population in this study were all 164 preeclampsia patients from August to October 2020. Result:Test data analysis using testwilcoxon test (? = 0.05) in the cucumber group, it was found that p = 0.000 on decreased hemoglobin and increased platelets Conclusion:There is an effect of cucumber on decreasing hemoglobin and increasing platelets in preeclampsia patients at Dewi Sartika General Hospital.

scholarly journals Comparison of mice’ sperm parameters exposed to some hazardous physical agents

2021 ◽  
Vol 36 (3) ◽  
pp. e2021013
Author(s):  
Mohammad-Bagher Abdollahi ◽  
Somayeh Farhang Dehghan ◽  
Faezeh Abasi Balochkhaneh ◽  
Manouchehr Ahmadi Moghadam ◽  
Hamzeh Mohammadi
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Field Studies ◽  
Sperm Parameters ◽  
World Health ◽  
Control Group ◽  
Normal Morphology ◽  
Progressive Motility ◽  
Noise Vibration ◽  
Health Organization

The present study was aimed to compare the effects of exposure to noise, vibration, lighting, and microwave on male mice’ sperm parameters. The mice were randomly assigned to five groups of eight, which comprised of the unexposed group and exposure groups including the lighting (1000 lux), noise (100 dB(A)), vibration (acceleration of 1.2 m/s2) and microwave (power density of 5 watts). The exposure groups were subjected to the four agents for 8 hours a day, 5 days a week during a 2-week period. Semen analysis were done according to World Health Organization guidelines. The highest significant mean difference in sperm count (-1.35×106/mL) had being observed between the microwave group and the control one (P=0.001). The highest difference in immotile percent (25.88 %) had being observed between the noise group and the control one (P=0.001). The highest difference in normal morphology (-27.06 %) observed between the lighting exposure group and the control group (P=0.001). The four agents can cause changes in different sperm parameters, however for definite conclusion; more laboratory and field studies are required. In total, exposure to microwave has had the greatest effect on sperm count and exposure to light has had the greatest effect on normal morphology and non-progressive motility. Moreover, exposure to noise has had the greatest effect on progressive motility and immotile percent, respectively.

scholarly journals The Effect of COVID-19 Infection on Sperm Quality and Male Fertility

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Hamid Piroozmanesh ◽  
Ebrahim Cheraghi ◽  
Leila Naserpoor ◽  
Masoumeh Aghashahi ◽  
Rahil Jannatifar
Keyword(s):  
Sperm Concentration ◽  
Reproductive Hormones ◽  
Sperm Parameters ◽  
World Health ◽  
Control Group ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Rt Pcr ◽  
Sperm Dna Integrity ◽  
Sperm Dna

Background: Coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may lead to the infertility of men. Objectives: This study aimed to investigate the impact of COVID-19 infection on sperm parameters and reproductive hormones in fertile men. Methods: A total of 100 males were selected and divided into two groups: (1) patients in convalescence (patients suffering from COVID-19 infection in pharyngeal swab in accordance with reverse-transcription polymerase chain reaction [RT-PCR] or antibodies); (2) negative control group (without antibodies). Semen and blood samples were gathered from all subjects. In the native semen, immunoglobulin (Ig) M and IgG antibodies in the blood were confirmed, and COVID-19 was detected via RT-PCR. To this end, based on the World Health Organization (WHO) guidelines, semen analysis, total antioxidant capacity (TAC), and sperm DNA integrity were assessed. Results: Results demonstrated that sperm concentration, motility, sperm viability, and TAC significantly reduced in fertile males with virus infection. In comparison with the control group, sperm DNA integrity was significantly increased (P < 0.05). Data indicated that the semen volume was not significantly correlated with COVID-19, and there was a significantly negative correlation between sperm concentration, sperm total motility, sperm vitality, sperm normal forms, and TAC with COVID-19. Sperm DNA fragmentation index had a significant and positive correlation with COVID-19 (P < 0.05). In addition, reproductive hormones significantly reduced in fertile males with COVID-19 infection (P < 0.05). Conclusions: COVID-19 infection has a negative influence on sperm parameters and reproductive hormones in fertile males.

scholarly journals Prion diseases are under compulsory notification in Brazil: Surveillance of cases evaluated by biochemical and/or genetic markers from 2005 to 2007

2007 ◽  
Vol 1 (4) ◽  
pp. 347-355 ◽  
Author(s):  
Vilma Regina Martins ◽  
Hélio Rodrigues Gomes ◽  
Leila Chimelli ◽  
Sergio Rosemberg ◽  
Michele Christine Landemberger
Keyword(s):  
Prion Diseases ◽  
World Health ◽  
Control Group ◽  
The United Kingdom ◽  
New Variant ◽  
The World ◽  
Jakob Disease ◽  
Health Organization ◽  
Compulsory Notification ◽  
Codon 129

Abstract The emergence of the new variant of Creutzfeldt-Jakob disease (vCJD) in the United Kingdom has raised concerns over the risks of this prion disease in other parts of the world. Since 2005, human prion diseases have been under compulsory notification in Brazil. It is well known that some polymorphisms within the cellular prion gene (PRNP) have been associated to a higher susceptibility to sporadic CJD (sCJD) and vCJD. Objectives: To describe the first notified cases and to evaluate the presence of mutations and polymorphisms of the PRNP in these cases. Methods: Thirty-five notified cases were evaluated by clinical, auxiliary exams and biochemical and/or genetic tests and classified according to the World Health Organization criteria for CJD. A control group (N=202) was included for the purpose of comparing the genetic analyses. Results: Twenty seven cases (74%) were classified as possible sCJD while 51% fulfilled the criteria for probable sCJD. Brain tissue analysis was available in three cases, where two were classified as definite sCJD and one as unconfirmed sCJD. Mutation of the PRNP was not found, and regarding the codon 129 polymorphism, valine in both alleles (Val129Val) was more frequent in patients than in the control group (OR=4.98; 1.55-15.96; p=0.007) when all possible cases were included, but not when only probable cases were considered. Conclusions: Our data did not show correlation of PRNP polymorphisms with probable sCJD cases. It is necessary to work toward notification of all cases of possible CJD in Brazil and to increase the rate of definitive diagnoses.

Low-power laser in the prevention of induced oral mucositis in bone marrow transplantation patients: a randomized trial

Blood ◽  
2006 ◽  
Vol 109 (5) ◽  
pp. 2250-2255 ◽  
Author(s):  
Héliton Spíndola Antunes ◽  
Alexandre Mello de Azevedo ◽  
Luiz Fernando da Silva Bouzas ◽  
Carlos Alberto Esteves Adão ◽  
Claudia Tereza Pinheiro ◽  
...  
Keyword(s):  
Low Power ◽  
Oral Mucositis ◽  
World Health ◽  
Control Group ◽  
Clinical Effects ◽  
Power Laser ◽  
Hematopoietic Stem ◽  
Section Area ◽  
The World ◽  
Health Organization

Abstract We investigated the clinical effects of low-power laser therapy (LPLT) on prevention and reduction of severity of conditioning-induced oral mucositis (OM) for hematopoietic stem cell transplantation (HSCT). We randomized 38 patients who underwent autologous (AT) or allogeneic (AL) HSCT. A diode InGaAlP was used, emitting light at 660 nm, 50 mW, and 4 J/cm2, measured at the fiberoptic end with 0.196 cm2 of section area. The evaluation of OM was done using the Oral Mucositis Assessment Scale (OMAS) and the World Health Organization (WHO) scale. In the LPLT group, 94.7% of patients had an OM grade (WHO) lower than or equal to grade 2, including 63.2% with grade 0 and 1, whereas in the controls group, 31.5% of patients had an OM grade lower than or equal to grade 2 (P < .001). Remarkably, the hazard ratio (HR) for grades 2, 3, and 4 OM was 0.41 (range, 0.22-0.75; P = .002) and for grades 3 and 4 it was 0.07 (range, 0.11-0.53; P < .001). Using OMAS by the calculation of ulcerous area, 5.3% of the laser group presented with ulcers of 9.1 cm2 to 18 cm2, whereas 73.6% of the control group presented with ulcers from 9.1 cm2 to 18 cm2 (P = .003). Our results indicate that the use of upfront LPLT in patients who have undergone HSCT is a powerful instrument in reducing the incidence of OM and is now standard in our center.

Effect of Vitamin C, Vitamin E, Zinc, Selenium, and Coenzyme Q10 in Infertile Men with Idiopathic Oligoasthenozoospermia

2017 ◽  
Vol 8 (2) ◽  
pp. 45-49 ◽  
Author(s):  
Ahmed T Alahmar
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Coenzyme Q10 ◽  
Seminal Fluid ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
World Health ◽  
Infertile Men ◽  
Semen Volume ◽  
Health Organization

ABSTRACT Introduction Accumulating evidence suggests that oxidative stress plays an important role in the development of male infertility and recently antioxidants have been tried to treat men with idiopathic infertility. Objective To assess the effect of treatment with vitamin C, vitamin E, zinc, selenium, and coenzyme Q10 on seminal fluid parameters in infertile men with idiopathic oligoasthenozoospermia. Materials and methods A prospective randomized trial was conducted on 32 infertile men with idiopathic oligoasthenozoospmia who received a daily supplement of one caplet containing vitamin C (90 mg/day), vitamin E (15 mg/day), coenzyme Q10 (4 mg/day), selenium (30 µg/day), and zinc (5 mg/day) for 3 months. Semen analysis was performed at baseline and 3 months after treatment using World Health Organization (WHO) 2010 guidelines. Results Significant improvement in sperm concentration was observed after combination therapy (9.13 ± 4.29 vs 11.3 ± 6.05 × 106/mL, p < 0.05). Sperm progressive motility (18.1 ± 8.68 vs 24.6 ± 10.2%, p < 0.01) and total motility (28.4 ± 8.71 vs 34.4 ± 11.7%, p < 0.01) also increased significantly following treatment. No change, however, was observed in semen volume or the proportion of sperms with normal morphology. Conclusion The combination of vitamin C, vitamin E, zinc, selenium, and coenzyme Q10 can significantly improve sperm concentration and motility in infertile men with idiopathic oligo­asthenozoospermia, which could be attributed to their synergistic antioxidant action. How to cite this article Alahmar AT. Effect of Vitamin C, Vitamin E, Zinc, Selenium, and Coenzyme Q10 in Infertile Men with Idiopathic Oligoasthenozoospermia. Int J Infertil Fetal Med 2017;8(2):45-49.

scholarly journals LA FENILCETONURIA Y LA HIPERACTIVIDAD: RESULTADOS EN NIÑOS EXTREMEÑOS

2016 ◽  
Vol 1 (2) ◽  
pp. 253
Author(s):  
Virgilio García Aparicio ◽  
Florencio Vicente Castro
Keyword(s):  
Total Population ◽  
Development Stage ◽  
World Health ◽  
Control Group ◽  
Severe Mental Retardation ◽  
Exclusion Criteria ◽  
Hyperactivity Disorder ◽  
The World ◽  
Phenylalanine Metabolism ◽  
Health Organization

RESUMEN.La Fenilcetonuria (PKU) pertenece a un tipo de hiperfenilalaninemias, éstas son un grupo de errores innatos en el metabolismo del aminoácido fenilalanina, que se caracterizan normalmente por producir un retraso mental severo e incluso la muerte cuando no se tratan adecuadamente. De acuerdo con la Organización Mundial de la Salud (OMS), está catalogada como enfermedad rara. Por otro lado, el Trastorno por Déficit de Atención con Hiperactividad (TDAH) es uno de los trastornos con mayor prevalencia en niños en edad escolar. Consiste en un patrón persistente de déficit que incluye la falta de atención y/o hiperactividad e impulsividad, y se presenta con una frecuencia y una gravedad que es inconsistente y no adaptativa en relación con el nivel de desarrollo del niño (APA, 2000).Objetivo: Comprobar que los niños fenilcetonúricos obtienen resultados significativamente superiores a los niños del grupo control para los indicadores de TDAH.Metodología: la investigación contempla un carácter descriptivo y cuantitativo, se utilizaron las herramientas de entrevista y el cuestionario Conners (padres y profesores) en una muestra de ocho niños extremeños fenilcetonúricos, que representan la población total de fenilcetonúricos en el rango de edad de entre ocho a trece años después de tener en cuenta los criterios de exclusión, y ocho niños extremeños de edad similar como grupo control.ABSTRACT.Phenylketonuria (PKU) is a type of hyperphenylalaninemias, which are an inborn group of errors of the amino acid phenylalanine metabolism that are usually characterized by producing a severe mental retardation and even death if it is not treated properly.According to the World Health Organization (WHO), it is classified as rare disease. On the other hand, Attention Deficit Hyperactivity Disorder (ADHD) is one of the most prevalent disorders in  schoolchildren. It consists of a persistent deficit pattern that includes lack of attention and / or hyperactivity and impulsivity, and it is present with an inconsistent and non adaptative frequency and severity with regard to the child's  development stage (APA, 2000).Objective: To check that PKU children get significantly higher results than the control group of children for ADHD indicators.Methodology: the research includes a descriptive and quantitative approach. Interview tools were used as well as Conners Questionnaire (parents and teachers) in a sample of eight phenylketonuric children from Extremadura, who represents the total population of PKU children aged 8–13, taking into account the exclusion criteria, and eight children from Extremadura aged similarly as control group.

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