scholarly journals Comparison of embryonic competence and clinical outcomes between early and late cumulus cell removal for in vitro fertilization

2021 ◽  
Author(s):  
Pallop Pongsuthirak
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Pregnancy Outcomes ◽  
Cumulus Cell ◽  
Vitro Fertilization ◽  
Group I ◽  
Group Ii ◽  
The Impact ◽  
Rescue Icsi

Objective: The impact of early mechanical removal of cumulus cells on fertilization and embryonic development is not yet precisely known. This study aimed to investigate the effects of early and late cumulus cell removal on fertilization, polyspermy, embryonic development potential, blastocyst development, and clinical outcomes.Methods: A prospective study was conducted of patients who underwent in vitro fertilization between September 2019 and October 2020. Sibling oocytes were randomly allocated after insemination to early cumulus cell removal at 6 hours (group I) and late cumulus cell removal at 16–18 hours (group II). If total fertilization failure (TFF) was determined to have occurred at early cumulus cell removal, rescue intracytoplasmic sperm injection (ICSI) was performed. Fertilization, embryonic development, and pregnancy outcomes were compared.Results: A total of 912 oocytes were assigned to group I (458 oocytes) and group II (454 oocytes). Fertilization, polyspermy, embryo quality, and pregnancy outcomes were not significantly different between both groups. Rescue ICSI enabled fertilization of 79.2% of the TFF oocytes.Conclusion: Early cumulus cell removal at 6 hours had no significant difference in fertilization, polyspermy, embryo development, or obstetric and perinatal outcomes compared to late removal. Early cumulus cell removal combined with early rescue ICSI may have the potential to help couples with TFF.

scholarly journals Comparison of short and long co-incubation time of gametes for in vitro fertilization

2019 ◽  
Vol 8 (11) ◽  
pp. 4366
Author(s):  
Pallop Pongsuthirak
Keyword(s):  
In Vitro Fertilization ◽  
Incubation Time ◽  
Pregnancy Outcomes ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Group I ◽  
Significant Difference ◽  
Total Fertilization Failure ◽  
Rescue Icsi

Background: The short and long co-incubation time of gametes for in vitro fertilization are still debatable issues. This study aims to investigate the effects of short and long co-incubation time of gametes on fertilization, polyspermy, embryonic developmental potential, and clinical outcomes.Methods: Sixty-five patients undergoing IVF treatment were invited to participate in the study between May 2017 and March 2019. Ovarian hyperstimulation was prescribed and oocytes were obtained by trans-vaginal aspiration under ultrasound guidance. Sibling oocytes were randomly allocated to short co-incubation for 4 hours (Group I) in 352 oocytes and long co-incubation for 16-18 hours in 363 oocytes (Group II). Rescue ICSI was carried out if total fertilization failure was documented. Fertilization, embryonic development, and pregnancy outcomes were determined.Results: No significant differences between short and long co-incubation were found in fertilization, polyspermy, cleavage, blastocyst, implantation, clinical pregnancy, and live birth rates.Conclusions: The present study showed that short co-incubation of gametes had no significant difference in fertilization, polyspermy, embryo development, and pregnancy outcomes when compared to long co-incubation. The short co-incubation with early cumulus cell removal and rescue ICSI may have the potential to help a couple who had total fertilization failure.

scholarly journals SUN-715 IIM May Influence Matured Oocytes’ DNA Methylation of PCOS Patients

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Congru Li ◽  
Yang Yu
Keyword(s):  
Dna Methylation ◽  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Embryo Transfer ◽  
Cpg Island ◽  
Reproductive Technologies ◽  
Childbearing Age ◽  
Vitro Fertilization ◽  
The Impact

Abstract Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of childbearing age and is the main cause of anovulatory infertility. To increase the number of oocytes obtained, controlled ovarian stimulation (COS) has become a routine choice for in vitro fertilization-embryo transfer (IVF-ET), which is one of the common assisted reproductive technologies for PCOS patients. However, for these patients, there is a high risk of ovarian hyperstimulation syndrome (OHSS). Obtaining in vitro maturation (IVM) of immature oocytes, and then in vitro fertilization and embryo transfer of mature oocytes provides a possible way for people to solve the above problems. Since the IVM technology will expose oocytes to in vitro conditions for a longer period of time, theoretically increasing the risk of the oocytes being affected by the culture environment, further research and explorations are needed for study in gene programming, epigenetics, etc. Therefore, to explore the impact of IVM operation on embryonic development is of great significance for further clarifying assisted reproductive safety and improving IVM operation conditions. Here we focused on DNA methylation reprogramming process which was essential for embryonic development. We tested the DNA methylation of sperm, IVM oocytes and IVM generated early stage embryos including pronucleus, 4cell, 8cell, morula, inner cell mass, trophoectoderm (TE) as well as six-week embryos by Nimble Gen Human DNA Methylation 3x729K CpG Island Plus RefSeq Promoter Array and compared the data with our published genome-wide DNA methylomes of human gametes and early embryos generated from in vivo maturation oocytes. We showed that IVM embryos show abnormal DNA methylation reprogramming pattern. By analyzing the abnormally reprogrammed promoters, we further found that IVM may affect the functions of demethylation related genes. Oocytes from IVM manipulation were tested with higher DNA methylation levels, and their abnormal methylated promoters mainly enriched in immune and metabolism pathways. Furthermore, we investigated the DNA methylation of TE, which was directly related with implantation process and revealed the abnormal methylated promoters were related with metabolism pathway too. Our data support that IVM may influence the DNA methylome of oocytes, which in turn affects the methylome of their embryos. However, due to the limited number of samples and the inability of the chip to cover all CpG sites, the results of this study require further research and validation.

scholarly journals The impact of atosiban on pregnancy outcomes in women undergoing in vitro fertilization-embryo transfer: A meta-analysis

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175501 ◽  
Author(s):  
Qian-Yi Huang ◽  
Min-Hua Rong ◽  
Ai-Hua Lan ◽  
Xiao-Miao Lin ◽  
Xing-Gu Lin ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Pregnancy Outcomes ◽  
Meta Analysis ◽  
Vitro Fertilization ◽  
The Impact

scholarly journals The Impact of the Coronavirus Disease 19 Pandemic on Early Pregnancy Outcomes Among Patients Undergoing In Vitro Fertilization Treatment

2021 ◽  
Vol 2 (1) ◽  
pp. 473-478
Author(s):  
Devora Aharon ◽  
Dmitry Gounko ◽  
Joseph A. Lee ◽  
Alan B. Copperman ◽  
Eric Flisser
Keyword(s):  
In Vitro Fertilization ◽  
Early Pregnancy ◽  
Pregnancy Outcomes ◽  
Vitro Fertilization ◽  
The Impact

333 EFFECTS OF BOVINE OOCYTE QUALITY ON KINETICS OF NUCLEAR MATURATION AND EMBRYONIC DEVELOPMENT AFTER IN VITRO FERTILIZATION

2010 ◽  
Vol 22 (1) ◽  
pp. 322
Author(s):  
I. Carvalhais ◽  
M. Faheem ◽  
A. Habibi ◽  
A. Geraldo ◽  
R. Agrícola ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryonic Development ◽  
Cumulus Cells ◽  
Oocyte Quality ◽  
Blastocyst Stage ◽  
Morphological Aspect ◽  
Vitro Fertilization ◽  
Nuclear Maturation ◽  
Group I

Many factors act together to prepare the immature oocyte for successful development to a competent embryo after fertilization. Defects in oocyte maturation and further development can possibly be caused by the oocyte quality or an inadequate nuclear maturation or even by a failure of both. In the present study, the effect of COCs quality on meiotic development and further embryo-development after in vitro fertilization was evaluated. A total of 3604 COCs was separated according to their morphological aspect and were classified as A, B, and C categories. Briefly, in class A, oocytes possessed compact layers of cumulus cells, being difficult to evaluate their number having a homogenous ooplasm with uniform color. In class B, oocytes show more or equal to five layers of cumulus cells, easily identifiable under a stereomicroscope and/or granulations in the ooplasm. In class C, some granulation was observed in oocytes with about three layers of cumulus cells. The total number of oocytes was divided into two groups (I and II) in which in the group I, COCs (n = 540) were fixed 0, 6, 12, 18, 24, and 30 h following ovarian aspiration, DNA was stained with aceto-orcein, and the nucleus were observed under a phase contrast microscope. In the Group II, COCs (n = 3064) were fertilized with frozen/thawed bull semen after 24 h of maturation, which was made in M199 medium (Sigma, St, Louis, MO, USA). The development of the embryos was evaluated on the third and seventh day after fertilization. Embryos were co-cultured with monolayers of granulosa cells in 45 μL droplets of B2 medium (CCD Laboratory, Paris, France), supplemented with 10% serum under mineral oil, at 39°C and 5% CO2 in air. It was observed that, other than the oocytes achieved metaphase II at 24 h was greater for the oocytes classified as A (65.4%), and B (61.0%) greater than C (51.2%), no statistical difference was observed between oocyte quality and capability to maturation. As far as the embryonic development is concerned, the same tendency was observed for the cleavage and for the morulae/blastocyst stage after 7 days after fertilization (P < 0.001). The percentages of cleaved oocytes classified as A, B, and C, were respectively 65.2%, 58.4%, and 48.0%. The development to the morulae/blastocyst stage of the cleaved embryos was A = 38.5%/27.4%, B = 33.6%/25.0%, and C = 30.9%/17.2% (Table 1). The results of our study clearly demonstrate that the morphology of the oocytes plays an important role on the in vitro embryonic developmental competence after fertilization. Table 1.Development of oocytes according to COCs quality, evaluated 3 and 7 days after fertilization The first author is supported by the Regional Foundation for Science and Technology of the Azores Government. This study was supported by the IBBA Institute grant number M2.1.2/I/022/2008 CITA-A is fully acknowledged.

scholarly journals The relationship between uterine corpus inclination and the outcomes of in vitro fertilization and embryo transfer: a retrospective study

2021 ◽  
Author(s):  
Xiuxia Wang ◽  
Na Zuo ◽  
Ning-Ning Zhang ◽  
Sitong Dong ◽  
Wei Zhang ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Pregnancy Outcomes ◽  
Average Distance ◽  
Transvaginal Ultrasound ◽  
Cleavage Stage ◽  
Vitro Fertilization ◽  
Uterine Corpus ◽  
The Impact

Objective: To investigate the impact of the uterine corpus inclination on pregnancy outcomes in in vitro fertilization and embryo transfer (IVF-ET). Design: Retrospective cohort study. Setting: University-based reproductive medicine center. Population: 526 patients underwent their first frozen embryo transfer (FET) cycle. Methods: All patients underwent transvaginal ultrasound examination to measure the distance from the midline of uterine cavity to the ultrasound probe, which indirectly reflect the uterine corpus inclination. The uterine corpus tends toward the horizontal position as the average distance increases. Multivariable regression analysis was used to study the effect of the uterine corpus inclination on pregnancy outcomes, even in different embryo stage cohorts as subgroup analysis. Main outcomes measures: Clinical pregnancy rate (CPR), spontaneous abortion rate, and live birth rate (LBR). Results:The patients were grouped according to the quartiles of the average distance distribution (Group 1, ≤ 1.98 cm; Group 2, 1.99-2.19 cm; Group 3, 2.20-2.50 cm; Group 4, ≥ 2.51 cm). There were statistically significant differences in the CPR and LBR between Groups 1 and 4 in the cleavage-stage ET cohort (CPR: adjusted odds ratio [OR] 0.225, 95% confidence interval [CI] 0.068-0.774; LBR: adjusted OR 0.315, 95% CI 0.100-0.996) (P < 0.05). There were no significant differences among the four groups in pregnancy outcomes in the blastocyst transfer cohort (P > 0.05). The cut-off value of 2.146 cm was calculated to predict the pregnancy outcomes in the cleavage-stage ET cohort. Conclusion:The uterine corpus inclination might be an independent risk factor for the success of cleavage-stage ET

scholarly journals BIOADAPTIVE MANAGEMENT IN CORRECTION OF PSYCHOEMOTIONAL DISORDERS IN WOMEN WITH INFERTILITY AND ENDURED PRENATAL STRESS

2021 ◽  
Author(s):  
Semenenko I. V.
Keyword(s):  
In Vitro Fertilization ◽  
Prenatal Stress ◽  
Depression Scale ◽  
Comprehensive Treatment ◽  
Biological Feedback ◽  
Vitro Fertilization ◽  
Group I ◽  
Significant Difference ◽  
Group Ii

Treatment of non-motor disorders in patients with infertility and prenatal stress (PS) is an important component of comprehensive treatment of the disease. This study examined the clinical effectiveness of a non-drug method of bioadaptive management - training based on biological feedback (BFB) in the correction of psychoemotional disorders in patients with infertility and prenatal stress. Object and methods of research. The study involved 41 patients with infertility and PS aged 22 to 45 years. Group I consisted of 21 women who underwent infertility treatment using in vitro fertilization and biological feedback, group II - 20 women who underwent treatment using in vitro fertilization without biological feedback. All patients were assessed for psychoemotional status on the Beck Depression Scale (BDI-II), tests for Spielberger-Khanin reactive anxiety, assessment of personal anxiety on the 1st day of observation, at the end of the course of BFB - training in group I and after 10±2 days in the comparison group. Results. There was a significant overall improvement in the psycho-emotional state of patients who underwent a full course of BFB - training at the end of therapy (p<0,05). There was a statistically significant difference between the level of certain psychoemotional disorders (in particular, anxiety, reduced stress resistance) of the studied groups (p<0,05). However, in contrast to group I patients who underwent BOS training, no statistically significant improvement in the psychoemotional status of group II patients during the observation period was found. Conclusions. BFB training can be considered as an appropriate component in the comprehensive treatment of psychoemotional disorders in patients with infertility and prenatal stress.

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