Evaluation of Secondary Acid and Enzymatic Hydrolysis of Hemicellulose in Hot Water Pre-Pulping Extract of Mixed Hardwoods

AbstractBackground:Plantago majorL. leaves have been used for centuries by the traditional medicine in the treatment of infectious disorders of the respiratory, urinary and digestive tracts. Researchers have reported that hot water extracts ofPlantago majorpossess a broad-spectrum of anticancer, antioxidant and antiviral activities, as well as activities which modulate cell-mediated immunity. Their beneficial properties may be due to the significant content of polysaccharides. The polysaccharides that have been isolated from the leaves ofPlantago majorL. have different structures – pectic substances, galactans, arabinogalactans, glucomannans.Aim:The aim of this paper was to study the correlation between the structure of the water extractable polysaccharides isolated fromPlantago majorL. leaves and their enzymatic hydrolysis with different carbohydrate hydrolases.Materials and methods:The hydrolysis reactions were performed with the enzymes hemicellulase and mannanase. Spectrophotometric total reducing sugars assay was used to examine the hydrolysis yield. The monosaccharide and oligosaccharide compositions were determined using HPLC analysis.Results:The highest hydrolysis yield of the water extractable polysaccharides fromPlantago majorleaves was obtained by treatment with hemicellulase. The hydrolysis yield increased with the augmentation of the ratio of enzyme to polysaccharide. Galactose was the prevalent monosaccharide identified in the composition of the isolated polysaccharides. Oligosaccharides with different degree of polymerization were also detected.Conclusion:The enzymatic hydrolysis of water extractable polysaccharides fromPlantago majorleaves allows us to obtain different types of oligosaccharides with beneficial effects on both human health and industry.

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An on-line method for pressurized hot water extraction and enzymatic hydrolysis of quercetin glucosides from onions

Analytica Chimica Acta ◽

10.1016/j.aca.2013.04.031 ◽

2013 ◽

Vol 785 ◽

pp. 50-59 ◽

Cited By ~ 19

Author(s):

Sofia Lindahl ◽

Jiayin Liu ◽

Samiullah Khan ◽

Eva Nordberg Karlsson ◽

Charlotta Turner

Keyword(s):

Enzymatic Hydrolysis ◽

Hot Water ◽

Water Extraction ◽

Hot Water Extraction ◽

Pressurized Hot Water Extraction ◽

Line Method ◽

On Line ◽

Hydrolysis Of

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Fungal pretreatment and enzymatic hydrolysis of genetically-modified Populus trichocarpa

BioResources ◽

10.15376/biores.15.3.6488-6505 ◽

2020 ◽

Vol 15 (3) ◽

pp. 6488-6505

Author(s):

Charles W. Edmunds ◽

Perry Peralta ◽

Ratna R. Sharma-Shivappa ◽

Stephen S. Kelley ◽

Vincent L. Chiang ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Populus Trichocarpa ◽

Genetically Modified ◽

Fold Increase ◽

Hot Water ◽

Dilute Acid ◽

Glucose Yield ◽

Sugar Release ◽

Fungal Pretreatment ◽

Hydrolysis Of

Fungal pretreatment of Populus trichocarpa wood genetically modified to reduce lignin and alter lignin chemistry is investigated for its effectiveness as an alternative to common pretreatment methods. The goal of this work is to improve biomass utilization for biofuel and biochemical applications by increasing sugar release. Sugar release after enzymatic hydrolysis was measured after various biomass pretreatments (including wood-rot fungus, hot water, and dilute acid). In the wildtype, and in constructs downregulated in PAL, 4CL, and C3H, the fungal pretreatment resulted in substantial improvements in sugar yields, up to 2.4-fold increase in glucose yield and 6-fold increase in xylose yield after enzymatic hydrolysis compared to the unpretreated control. However, the effects of fungal pretreatment were inconsistent, and in genetic lines down-regulated in 4CL, CCoAOMT, CAld5H, and C3H, fungal pretreatment yielded similar or decreased sugar release after enzymatic hydrolysis.

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