Differential Proteome Analysis of Breast and Thigh Muscles between Korean Native Chickens and Commercial Broilers

SummaryEpidemiological studies indicate that the consumption of soy-containing food may prevent or slow-down the development of cardiovascular disease. In endothelial cells application of a soy extract or a combination of the most abundant soy isoflavones genistein and daidzein both inhibited apoptosis, a driving force in atherosclerosis development, when applied in combination with oxidized LDL or homocysteine. Proteome analysis revealed that the stressorinduced alteration of protein expression profile was reversed by the soy extract or the genistein/daidzein mixture. Only few protein entities that could be functionally linked to mitochondrial dysfunction were regulated in common by both application forms of isoflavones. A dietary intervention with isoflavone-enriched soy extract in postmenopausal women, who generally show strongly increased cardiovascular risk due to diminished estrogen production, led to significant alterations in the steady state levels of proteins from mononuclear blood cells. The proteins identified by proteome analysis revealed that soy isoflavones may increase the anti-inflammatory response in blood mononuclear cells thereby contributing to the atherosclerosispreventive activities of a soy-rich diet. Conclusion: By proteome analysis protein targets were identified in vitro in endothelial cells that respond to soy isoflavones and that may decipher molecular mechanisms through which soy products exert their protective effects in the vasculature.

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DIE INITIALE VERTEILUNG VON RADIO-C IN DEN ZELLFRAKTIONEN EINIGER RATTENORGANE NACH INJEKTION VON TESTOSTERON-4-14C

Acta Endocrinologica ◽

10.1530/acta.0.0470051 ◽

1964 ◽

Vol 47 (1) ◽

pp. 51-57 ◽

Cited By ~ 4

Author(s):

K.-O. Mosebach ◽

W. Dirscherl

Keyword(s):

Intraperitoneal Injection ◽

Specific Activity ◽

Initial Distribution ◽

Male Rats ◽

Initial Formation ◽

Thigh Muscles ◽

The Absolute ◽

Cell Fractions

ABSTRACT The initial distribution of radioactive C was studied in the cell fractions of the liver, kidney, testes and thigh muscles after intraperitoneal injection of testosterone-4-14C into 40 day old male rats. To make this possible, the absolute and specific activity values (μc/mg C) were determined. After both ten and twenty minutes the cytoplasm fractions possessed the highest activity values, the only exception being the specific activity of the liver cytoplasm ten minutes after injection when the microsomes of the liver showed a higher activity. After 20 min the mitochondria possessed the highest specific activity values among the corpuscular fractions. The specific activity values in the microsomes of all four organs studied were lower 20 min after the time of injection than after 10 min, a fact, which is suspected to be the result of the initial formation of conjugates in the microsomes.

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