Identification of Radiodegradation Products of Acebutolol and Alprenolol by HPLC/MS/MS

2015 ◽  
Vol 98 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Magdalena Ogrodowczyk ◽  
Katarzyna Dettlaff ◽  
Piotr Kachlicki ◽  
Barbara Marciniec
Keyword(s):  
Physicochemical Properties ◽  
High Energy ◽  
Al Content ◽  
High Energy Electrons ◽  
Β Blockers ◽  
Solid Form ◽  
Derivatives Of ◽  
Higher Doses ◽  
Sterilization Dose ◽  
Additional Product

Abstract Two therapeutically active compounds from the group of β-blockers, acebutolol (AC) and alprenolol (AL), in solid form were subjected to ionizing radiation emitted by a beam of high energy electrons from an accelerator with a standard sterilization dose of 25 kGy and in higher doses of 50–400 kGy. The effects of irradiation were detected by chromatographic methods (TLC, HPLC) and a hyphenated method (HPLC/MS/MS). No significant changes in the physicochemical properties of both compounds studied irradiated with 25 kGy were noted, but upon irradiation with the highest dose (400 kGy) the loss of AC and AL content determined by HPLC was 2.79 and 9.12%, respectively. The product of AC decomposition and the two products of AL decomposition were separated and identified by HPLC/MS/MS. It has been established that radiodegradation of AC and AL takes place by oxidation, leading to formation of the products of radiolysis, most probably alcohol derivatives of the β-blockers studied. The additional product that appears on radiodegradation of AL is probably formed as a result of two simultaneous reactions: oxidation and CH2 group elimination.

scholarly journals Modeling of the irradiation effect on some physicochemical properties of metoprolol tartrate for safe medical uses

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Najoua Ouerfelli ◽  
Narcisa Vrinceanu ◽  
Ezzedine Mliki ◽  
Abdelgadir M. Homeida ◽  
Kamal A. Amin ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Solid Phase ◽  
High Energy ◽  
Irradiation Effect ◽  
Metoprolol Tartrate ◽  
Experimental Conditions ◽  
Peculiar Behaviour ◽  
High Energy Electrons ◽  
State Quality

AbstractThe effect of gamma-irradiation and ionizing radiation (high-energy electrons beam) on the physicochemical properties of metoprolol tartrate at the solid phase and aqueous solution, has been investigated in the present study to model some properties affected by absorbed doses and to reveal some interesting mutual causal correlation. The proposed some interesting models can be adapted to other experimental conditions, and the newly obtained values of the adjustable parameters could be an excellent criterion of the state quality of the metoprolol tartrate or for other additional interpretations. The peculiar behaviour of variation of physicochemical properties against dose leads us to confirm the suggested optimized doses mentioned in previous work, for sterilization and safe medical uses.

scholarly journals Effect of Radiation Sterilization on Alprenolol in the Solid State Studied by High-Performance Thin-Layer Chromatography

2010 ◽  
Vol 93 (3) ◽  
pp. 792-797
Author(s):  
Barbara Marciniec ◽  
Magdalena Ogrodowczyk ◽  
Anna Kwiecien′
Keyword(s):  
High Performance ◽  
Good Accuracy ◽  
High Energy ◽  
Degree Of Crystallinity ◽  
Radiation Sterilization ◽  
Good Precision ◽  
Solid Form ◽  
Layer Chromatography ◽  
Higher Doses ◽  
The Degree Of Crystallinity

Abstract The possibility of radiation sterilization of alprenolol (AL) has been studied. Irradiation of AL in solid form with a 25 kGy beam of electrons caused only an insignificant change in color that became more intense with increasing irradiation dose. Moreover, with increasing dose a decrease in pH, the content of water, and the degree of crystallinity were observed. AL in solid form was radiated with a high-energy electron beam (9.96 MeV) at doses from 25400 kGy and analyzed by HPTLC using the mobile phase methanolammonia 25 (99 + 1, v/v). Densitometric analysis was carried out directly from chromatograms at 270 nm. The applied method was validated and characterized by good precision (RSD = 3.95); good accuracy (80 level 100.15, 100 level 99.99, and 120 level 104.44); and low LOD (LOD = 0.52 g/zone and LOQ = 1.55 g/zone). Chromatograms recorded for samples irradiated at the doses of 25 kGy were unchanged, but at higher doses (100400 kGy) additional peaks corresponding to the radiodegradation products appeared (Rf = 0.24 and Rf = 0.40). The decrease in the concentration of AL was proportional to the applied radiation dose, and for 400 kGy the concentration of AL was 90.23. The calculated radiolytic yield of the radiodegradation process was G(AL) = 7.12 107 mol/J.

scholarly journals Spectroscopic analysis of pindolol irradiated in the solid state

2014 ◽  
Vol 12 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Magdalena Ogrodowczyk ◽  
Barbara Marciniec ◽  
Piotr Kachlicki
Keyword(s):  
Free Radicals ◽  
Solid State ◽  
Spectroscopic Analysis ◽  
Standard Dose ◽  
High Energy ◽  
Ionising Radiation ◽  
High Energy Electrons ◽  
Radiolytic Decomposition ◽  
Ft Ir ◽  
Higher Doses

AbstractPindolol ((2RS)-(1-(1H-indol-4-iloxy)-3- [(1-metyloetylo)amino]-2-propanol) in substantia was exposed to ionising radiation emitted by high energy electrons from an accelerator, in the standard sterilisation dose of 25 kGy and in higher doses from the range 50–400 kGy. The effects of irradiation were checked by spectrometric methods (UV, MS, FT-IR, EPR) and hyphenated methods (HPLC-MS) and the results were referred to those obtained for non-irradiated sample. EPR results indicated the presence of free radicals in irradiated samples, in the amount of 1.36 × 1016 spin g−1 for 25 kGy and 3.70×1016 spin g−1 for 400 kGy. The loss of pindolol content determined by HPLC was 1.34% after irradiation with 400 kGy, while the radiolytic yield of the total radiolysis for this dose of irradiation was 2.69×107 mol J−1. By means of HPLC-MS it was possible to separate and identify one product of radiolytic decomposition, which probably is 2-((R)-3-(1H-indol-4-yloxy)-2-hydroxypropylamino)propan-1-ol formed upon oxidation. In the range of sterilisation doses (25–50 kGy), pindolol was found to show high radiochemical stability and would probably be safely sterilised by the standard dose of 25 kGy.

Utilisation of High-Energy Heat Sources in Magnesium Alloy Surface Layer Treatment

2013 ◽  
Vol 58 (2) ◽  
pp. 619-624 ◽  
Author(s):  
M. Szafarska ◽  
J. Iwaszko ◽  
K. Kudła ◽  
I. Łegowik
Keyword(s):  
Surface Layer ◽  
Magnesium Alloy ◽  
Physicochemical Properties ◽  
Treatment Effectiveness ◽  
High Energy ◽  
Alloy Surface ◽  
Heat Sources ◽  
X Ray ◽  
Additional Equipment ◽  
Alloy Surface Layer

The main aim of the study was the evaluation of magnesium alloy surface treatment effectiveness using high-energy heat sources, i.e. a Yb-YAG Disk Laser and the GTAW method. The AZ91 and AM60 commercial magnesium alloys were subject to surface layer modification. Because of the physicochemical properties of the materials studied in case of the GTAW method, it was necessary to provide the welding stand with additional equipment. A novel two-torch set with torches operating in tandem was developed within the experiment. The effectiveness of specimen remelting using a laser and the GTAW method was verified based on macro- and microscopic examinations as well as in X-ray phase analysis and hardness measurements. In addition, the remelting parameters were optimised. The proposed treatment methodology enabled the achieving of the intended result and effective modification of a magnesium alloy surface layer.

scholarly journals Properties of Elementary Particle Fluxes in Primary Cosmic Rays Measured with the Alpha Magnetic Spectrometer on the International Space Station

2019 ◽  
Vol 209 ◽  
pp. 01007
Author(s):  
Francesco Nozzoli
Keyword(s):  
Electron Flux ◽  
Space Station ◽  
Cosmic Ray ◽  
High Energy ◽  
Precision Measurements ◽  
High Energy Electrons ◽  
Electrons And Positrons ◽  
Positron Flux ◽  
Rigidity Dependence ◽  
Alpha Magnetic Spectrometer

Precision measurements by AMS of the fluxes of cosmic ray positrons, electrons, antiprotons, protons as well as their rations reveal several unexpected and intriguing features. The presented measurements extend the energy range of the previous observations with much increased precision. The new results show that the behavior of positron flux at around 300 GeV is consistent with a new source that produce equal amount of high energy electrons and positrons. In addition, in the absolute rigidity range 60–500 GV, the antiproton, proton, and positron fluxes are found to have nearly identical rigidity dependence and the electron flux exhibits different rigidity dependence.

scholarly journals Polar Middle Atmospheric Responses to Medium Energy Electron (MEE) Precipitation Using Numerical Model Simulations

Atmosphere ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 133
Author(s):  
Ji-Hee Lee ◽  
Geonhwa Jee ◽  
Young-Sil Kwak ◽  
Heejin Hwang ◽  
Annika Seppälä ◽  
...  
Keyword(s):  
Climate Model ◽  
Middle Atmosphere ◽  
Meridional Circulation ◽  
Stratospheric Ozone ◽  
High Energy ◽  
Chemical Changes ◽  
Temperature Changes ◽  
Mesospheric Temperature ◽  
High Energy Electrons ◽  
Circulation Changes

Energetic particle precipitation (EPP) is known to be an important source of chemical changes in the polar middle atmosphere in winter. Recent modeling studies further suggest that chemical changes induced by EPP can also cause dynamic changes in the middle atmosphere. In this study, we investigated the atmospheric responses to the precipitation of medium-to-high energy electrons (MEEs) over the period 2005–2013 using the Specific Dynamics Whole Atmosphere Community Climate Model (SD-WACCM). Our results show that the MEE precipitation significantly increases the amounts of NOx and HOx, resulting in mesospheric and stratospheric ozone losses by up to 60% and 25% respectively during polar winter. The MEE-induced ozone loss generally increases the temperature in the lower mesosphere but decreases the temperature in the upper mesosphere with large year-to-year variability, not only by radiative effects but also by adiabatic effects. The adiabatic effects by meridional circulation changes may be dominant for the mesospheric temperature changes. In particular, the meridional circulation changes occasionally act in opposite ways to vary the temperature in terms of height variations, especially at around the solar minimum period with low geomagnetic activity, which cancels out the temperature changes to make the average small in the polar mesosphere for the 9-year period.

The Effects of High-Energy Electrons on the Charging of Spacecraft Dielectrics

1979 ◽  
Vol 26 (6) ◽  
pp. 5101-5106 ◽  
Author(s):  
M. J. Treadaway ◽  
C. E. Mallon ◽  
T. M. Flanagan ◽  
R. Denson ◽  
E. P. Wenaas
Keyword(s):  
High Energy ◽  
High Energy Electrons

scholarly journals Ovaries of Chrysanthemum Irradiated with High-Energy Photons and High-Energy Electrons Can Regenerate Plants with Novel Traits

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1111
Author(s):  
Natalia Miler ◽  
Iwona Jedrzejczyk ◽  
Seweryn Jakubowski ◽  
Janusz Winiecki
Keyword(s):  
Total Dose ◽  
In Vitro Regeneration ◽  
High Energy ◽  
Mutation Breeding ◽  
Chrysanthemum Morifolium ◽  
Physical Factors ◽  
Strong Impact ◽  
Breeding Method ◽  
High Energy Electrons

Classical mutation breeding using physical factors is a common breeding method for ornamental crops. The aim of our study was to examine the utility of ovaries excised from irradiated inflorescences of Chrysanthemum × morifolium (Ramat.) as explants for breeding purposes. We studied the in vitro regeneration capacity of the ovaries of two chrysanthemum cultivars: ‘Profesor Jerzy’ and ‘Karolina’ preceded by irradiation with high-energy photons (total dose 5, 10 and 15 Gy) and high-energy electrons (total dose 10 Gy). Growth and inflorescence parameters of greenhouse acclimatized regenerants were recorded, and ploidy level was estimated with flow cytometry. The strong impact of genotype on regeneration efficiency was recorded—cultivar ‘Karolina’ produced only 7 viable shoots, while ‘Profesor Jerzy’ produced totally 428 shoots. With an increase of irradiation dose, the regeneration decreased, the least responsive were explants irradiated with 15 Gy high-energy photons and 10 Gy high-energy electrons. Regenerants of ‘Profesor Jerzy’ obtained from these explants possessed shorter stem and flowered later. The highest number of stable, color and shape inflorescence variations were obtained from explants treated with 10 Gy high-energy photons. Variations of inflorescences were predominantly changes of shape—from full to semi-full. New color phenotypes were dark yellow, light yellow and pinkish, among them only the dark yellow phenotype remained stable during second year cultivation. None of the regenerants were haploid. The application of ovaries irradiated within the whole inflorescence of chrysanthemum can be successfully applied in the breeding programs, provided the mother cultivar regenerate in vitro efficiently.

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