Spectrofluorometric Determination of Putrescine: Optimization of the Putrescine–Orthophthaldehyde Complex Using Spectrofluorometry

2016 ◽  
Vol 99 (6) ◽  
pp. 1642-1644
Author(s):  
Oladele Oyelakin ◽  
Moumouny Traoré ◽  
El Hadji Babacar Mbye ◽  
Abdourahmane Khonté ◽  
Lamine Cisse ◽  
...  
Keyword(s):  
Alkaline Medium ◽  
Fluorescence Intensity ◽  
Spectrofluorometric Determination

Abstract In alkaline medium, the complex formed between putrescine and orthophthalaldehyde was studied using spectrofluorescence. The derivative is kinetically stable 24 h after complexation. The stoichiometry of the complex is 1:1 at maximum fluorescence intensity, also 24 h after complexation.

Fluorometric and Spectrophotometric Determination of Pirbuterol Hydrochloride in Authentic and Dosage Forms

1985 ◽  
Vol 68 (6) ◽  
pp. 1222-1225
Author(s):  
Mohamed E Mohamed ◽  
Hassan Y Aboul-Enein
Keyword(s):  
Potentiometric Titration ◽  
Alkaline Medium ◽  
Spectrophotometric Determination ◽  
Fluorescence Intensity ◽  
Perchloric Acid ◽  
Authentic Sample ◽  
Dosage Forms ◽  
Fluorometric Method ◽  
The Mean

Abstract Pirbuterol hydrochloride has been assayed in alkaline medium by using a fluorometric method to measure fluorescence intensity at 372 nm with excitation at 310 nm and by the ▵A method at 242 nm.The linearity ranges are 0.5-4 μg/mL and 10-50 μg/mL, respectively. An authentic pirbuterol HC1 sample was analyzed by nonaqueous potentiometric titration using 0.1N perchloric acid, and the results were compared with those for fluorometric and AA methods. The mean percent recoveries for the authentic sample were 98.72 ± 1.13 and 99.24 ± 0.85, respectively. When applied to commercial capsules containing 10 mg and 15 mg each, the fluorometric method gave mean percent recoveries of 101.11 ± 1.05 and 98.12 ± 0.93; the ▵A method gave mean percent recoveries of 100.57 ± 0.83 and 97.80 ± 0.75, respectively.

scholarly journals Micellar Enhanced Spectrofluorometric Determination of Labetalol Through Complexation with Aluminium(III): Application to Dosage Forms and Biological Fluids

2007 ◽  
Vol 90 (4) ◽  
pp. 948-956 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Fluorescence Intensity ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Dosage Forms ◽  
Spectrofluorometric Determination ◽  
Experimental Parameters ◽  
Fluorescent Products ◽  
Good Agreement

Abstract Two simple, sensitive, and specific spectrofluorometric procedures have been developed for the determination of labetalol (LBT) in pharmaceuticals and biological fluids. LBT was found to react with Al3+ , both in acetate buffer of pH 4.5 (Procedure I) and borate buffer of pH 8.0 (Procedure II), to produce highly fluorescent stable complexes. The fluorescence intensity could be enhanced by the addition of sodium dodecyl sulfate, resulting in 3.5- and 2.7-fold increases in the fluorescence intensity for Procedures I and II, respectively. In both procedures, the fluorescence intensity was measured at 408 nm after excitation at 320 nm. The different experimental parameters affecting the development and stability of the fluorescent products were carefully studied and optimized. The fluorescence intensity-concentration plots were rectilinear over the range of 0.020.1 and 0.010.05 g/mL with a detection limit of 0.003 and 0.001 g/mL for Procedures I and II, respectively. The proposed method was successfully applied to commercial tablets containing LBT. The results were in good agreement with those obtained using a reference spectrofluorometric method. Furthermore, the method was applied for the determination of LBT in spiked human plasma, and the recovery (n = 4) was 93.30 2.62%. A proposal of the reaction pathway was postulated for Procedures I and II, respectively.

scholarly journals Spectrofluorometric Determination of Citalopram in Pharmaceutical Preparations and Spiked Human Plasma Using Organized Media

2006 ◽  
Vol 89 (5) ◽  
pp. 1288-1295 ◽  
Author(s):  
Dina T El-Sherbiny
Keyword(s):  
Aqueous Solution ◽  
Human Plasma ◽  
Fluorescence Intensity ◽  
Pharmaceutical Preparations ◽  
Citrate Buffer ◽  
Acidic Aqueous Solution ◽  
Sds Micelles ◽  
Spectrofluorometric Determination ◽  
Organized Media

Abstract The native fluorescence of citalopram (CIT) was (obtained in citrate buffer of pH 6.5 with and without β-cyclodextrin (β-CD) or sodium dodecyl sulfate (SDS) as fluorescence enhancers at 305 nm using 242 nm for excitation. Micellar systems of ionic and nonionic surfactants were investigated by measuring the fluorescence intensity of the analyte-surfactant system. In slightly acidic aqueous solution of pH 6.5, CIT was better incorporated in CDs and SDS micelles. The luminescence emission from CIT was found to be greatly enhanced by SDS micelles. The fluorescence intensity enhancements in CDs medium and in SDS as ionic surfactant relative to slightly acidic aqueous solution were 125 and 250%, respectively. Organized media-enhanced spectroflourometric methods were developed for the determination of CIT, in pure form as well as in pharmaceutical preparations. The fluorescence intensity-concentration plots were rectilinear over the ranges 0.06 to 0.64, 0.04 to 0.40, and 0.02 to 0.26 μg/mL with lower detection limits of 0.02, 0.01, and 0.007 μg/mL, either in citrate buffer only or in β-CD and SDS as organized media, respectively. Furthermore, the high sensitivity attained by using SDS as organized medium allowed in vitro spectrofluorometric determination of CIT in spiked human plasma. Interference from endogenous amino acids has been overcome by using the solid-phase extraction technique; the mean recovery (n = 5) was 100.1 ± 0.8%

Spectrofluorometric Determination of Putrescine Using Orthophthaldehyde as a Fluorophore: A Novel Method

2016 ◽  
Vol 99 (1) ◽  
pp. 170-173 ◽  
Author(s):  
Oladele Oyelakin ◽  
Moumouny Traoré ◽  
El Hadji Babacar Mbaye ◽  
Abdourahmane Konté ◽  
Lamine Cissé ◽  
...  
Keyword(s):  
Alkaline Medium ◽  
Maximum Intensity ◽  
Alkaline Media ◽  
Good Reproducibility ◽  
Aqueous Alkaline Medium ◽  
Equimolar Ratio ◽  
Spectrofluorometric Determination ◽  
Novel Method ◽  
Detection And Quantification

Abstract This paper concerns spectrofluorometric analysis of putrescine using orthophthaladehyde as a fluorophore in aqueous alkaline medium. Wavelengths of excitation and emission in acid, neutral, and alkaline media were different. There is a maximum intensity of fluorescence in alkaline medium 24 h after complexation compared with other media. Putrescine and orthophthaladehyde are used at an equimolar ratio, and the product is kinetically stable in alkaline medium. Calibration curves obtained gave limits of detection and quantification of 39 and 65 ng/mL, respectively. The correlation coefficient obtained in alkaline medium was 0.992 at pH 12. Results obtained largely showed a good reproducibility of our method.

scholarly journals Spectrofluorometric Determination of Certain Antihyperlipidemic Agents in Bulk and Pharmaceutical Preparations

2012 ◽  
Vol 27 ◽  
pp. 83-92 ◽  
Author(s):  
Ramzia I. El-Bagary ◽  
Ehab F. ElKady ◽  
Ahmed M. Kadry
Keyword(s):  
Simultaneous Determination ◽  
Fluorescence Intensity ◽  
Pharmaceutical Preparations ◽  
Native Fluorescence ◽  
Spectrofluorometric Determination ◽  
Spectrofluorometric Method ◽  
Pitavastatin Calcium ◽  
Antihyperlipidemic Agents ◽  
Rosuvastatin Calcium

A simple, rapid, and sensitive spectrofluorometric method was developed for the determination of three antihyperlipidemic drugs, namely, rosuvastatin calcium (RSV), ezetimibe (EZE), and pitavastatin calcium (PIT). The method is based on measuring the native fluorescence of the cited drugs at their optimum excitation and emission wavelengths. The fluorescence intensity was measured atλem 362 nm, 309 nm, and 373 nm upon excitation atλex 315 nm, 260 nm, and 245 nm for RSV, EZE, and PIT, respectively. The calibration graphs were linear over the concentration ranges 0.50–10.0, 0.25–4.0, and 0.10–3.00 μg mL−1for RSV, EZE, and PIT, respectively. Besides, a spectrofluorometric method for the simultaneous determination of RSV and EZE was developed. The fluorescence was measured atλem 309 nm for EZE and 432 nm for RSV upon excitation atλex 260 nm for both. The proposed methods were applied to the determination of the cited drugs either in bulk and pharmaceutical preparations.

Utility of 4-Chloro-7-Nitrobenzofurazan (NBD-Cl) for the Spectrophotometric and Spectrofluorometric Determination of Several Antihistamine and Antihypertensive Drugs

2013 ◽  
Vol 96 (5) ◽  
pp. 968-975 ◽  
Author(s):  
Soad S Abd El-Hay ◽  
Christa L Colyer ◽  
Wafaa S Hassan ◽  
Abdalla Shalaby
Keyword(s):  
Fluorescence Intensity ◽  
Drug Concentration ◽  
Antihypertensive Drugs ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Emission Wavelength ◽  
Pharmaceutical Dosage Forms ◽  
Spectrofluorometric Determination ◽  
Highly Sensitive

Abstract New, sensitive, and selective spectrophotometric and spectrofluorometric methods have been developed for determination of clemastine hydrogen fumarate (Clem), loratadine (Lor), losartan potassium (Los), and ramipril (Ram) in both pure form and pharmaceutical formulations using 4-chloro-7-nitrobenzofurazan (NBD-Cl), which is a highly sensitive chromogenic and fluorogenic reagent. The relation between absorbance at 470, 467, 471, and 469 nm and the concentration was linear over the ranges 5–35, 10–100, 10–90, and 10–120 μg/mL for Clem, Lor, Los, and Ram, respectively. The complexation products were also measured spectrofluorometrically at the emission wavelength 535 nm for Clem, Lor, and Ram and at 538 nm for Los with excitation at 477 and 452 nm for Clem and Lor, respectively, and 460 nm for both Los and Ram. The fluorescence intensity was directly proportional to the drug concentration over the ranges 0.05–0.5, 5–20, 1–6, and 2–15 μg/mL for Clem, Lor, Los, and Ram, respectively. The methods were successfully applied for the determination of the studied drugs in pharmaceutical dosage forms with excellent recovery.

scholarly journals Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1246
Author(s):  
Tengfei Wang ◽  
Hui Luo ◽  
Xu Jing ◽  
Jiali Yang ◽  
Meijun Huo ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Ph Dependence ◽  
Scale Up ◽  
Water Soluble ◽  
Jujube Fruit ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.

Spectrophotometric and Spectrofluorometric Determination of Heptaminol and mexiletine in Their Dosage Forms

1997 ◽  
Vol 30 (11) ◽  
pp. 2029-2043 ◽  
Author(s):  
Abdel Fattah M. El Walily ◽  
Fawzy A. El-Yazbi ◽  
Saeid F. Belal ◽  
Omayma Abdel-Razak
Keyword(s):  
Dosage Forms ◽  
Spectrofluorometric Determination

Use of 1-Hydroxy-2-Carboxyanthraquinone for the Spectrofluorometric Determination of Be(II)

1975 ◽  
Vol 8 (10) ◽  
pp. 753-761 ◽  
Author(s):  
F. Capitan ◽  
F. Salinas ◽  
L. M. Franquelo
Keyword(s):  
Spectrofluorometric Determination
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