scholarly journals Effect of Monocyte Locomotion Inhibitory Factor (MLIF) on the Activation and Production of Intracellular Cytokine and Chemokine Receptors in Human T CD4+ Lymphocytes Measured by Flow Cytometry

10.5772/36713 ◽  
2012 ◽  
Author(s):  
Sara Rojas-Dotor
Keyword(s):  
Flow Cytometry ◽  
Chemokine Receptors ◽  
Inhibitory Factor ◽  
Intracellular Cytokine ◽  
Monocyte Locomotion Inhibitory Factor ◽  
Cd4 Lymphocytes

scholarly journals Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes

2009 ◽  
Vol 42 (4) ◽  
Author(s):  
SARA ROJAS-DOTOR ◽  
JULIA PÉREZ-RAMOS ◽  
JUAN ANTONIO GIMÉNEZ-SCHERER ◽  
FRANCISCO BLANCO-FAVELA ◽  
GUADALUPE RICO-ROSILLO
Keyword(s):  
Chemokine Receptors ◽  
Inhibitory Factor ◽  
Monocyte Locomotion Inhibitory Factor ◽  
Cd4 Lymphocytes

Decreased percentage of CD4+ lymphocytes expressing chemokine receptors in bipolar disorder

2019 ◽  
Vol 31 (05) ◽  
pp. 246-251
Author(s):  
Izabela G. Barbosa ◽  
Natalia P. Rocha ◽  
Erica L. Vieira ◽  
Mehmet A. Camkurt ◽  
Rodrigo B. Huguet ◽  
...  
Keyword(s):  
Bipolar Disorder ◽  
Flow Cytometry ◽  
Chemokine Receptor ◽  
Peripheral Blood ◽  
Chemokine Receptors ◽  
Type I ◽  
Blood Draw ◽  
Immune Pathways ◽  
Age And Sex ◽  
Cd4 Lymphocytes

AbstractObjective:Although accumulating evidence supports the hypothesis that immune/inflammatory mechanisms are associated with the pathophysiology of bipolar disorder (BD), data about the profile of chemokines (chemotactic cytokines) and chemokine receptors are still scarce. The current study was designed to evaluate the expression of chemokine receptors on lymphocytes of patients with BD in comparison with controls.Methods:Thirty-three patients with type I BD (N= 21 in euthymia;N= 6 in mania/hypomania;N= 6 in depression) and 22 age- and sex-matched controls were subjected to clinical evaluation and peripheral blood draw. The expression of chemokine receptors CCR3, CCR5, CXCR4, and CXCR3 on CD4+and CD8+lymphocytes was assessed by flow cytometry.Results:Patients with BD had decreased percentage of CD4+CXCR3+(p= 0.024), CD4+CCR3+(p= 0.042), and CD4+CCR5+(0.013) lymphocytes in comparison with controls. The percentage of both CD4+and CD8+lymphocytes expressing the chemokine receptor CXCR4 was similar in patients with BD and controls. Likewise, the percentages of CD8+CXCR3+, CD8+CCR3+, and CD8+CCR5+lymphocytes were similar in patients with BD and controls.Conclusion:Our findings reinforce the hypothesis that immune pathways, especially involving CD4+lymphocytes, are involved in the physiopathology of BD.

Monocyte Locomotion Inhibitory Factor confers neuroprotection and prevents the development of murine cerebral malaria

2021 ◽  
Vol 97 ◽  
pp. 107674
Author(s):  
A. Galán-Salinas ◽  
G. Corral-Ruíz ◽  
M.J. Pérez-Vega ◽  
L. Fabila-Castillo ◽  
R. Silva-García ◽  
...  
Keyword(s):  
Cerebral Malaria ◽  
Inhibitory Factor ◽  
Monocyte Locomotion Inhibitory Factor

scholarly journals Intracellular Cytokine Staining and Flow Cytometry: Considerations for Application in Clinical Trials of Novel Tuberculosis Vaccines

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138042 ◽  
Author(s):  
Steven G. Smith ◽  
Kaatje Smits ◽  
Simone A. Joosten ◽  
Krista E. van Meijgaarden ◽  
Iman Satti ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Clinical Trials ◽  
Intracellular Cytokine Staining ◽  
Intracellular Cytokine

scholarly journals Intracellular Cytokine Detection by Flow Cytometry in Surface Marker‐Defined Human Peripheral Blood Mononuclear T Cells

2017 ◽  
Vol 73 (1) ◽  
Author(s):  
Fredine T. Lauer ◽  
Jesse L. Denson ◽  
Ellen Beswick ◽  
Scott W. Burchiel
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Surface Marker ◽  
Intracellular Cytokine ◽  
Peripheral Blood Mononuclear ◽  
Cytokine Detection

Cytokine expression in CD4+ cells exposed to the monocyte locomotion inhibitory factor produced by Entamoeba histolytica

2005 ◽  
Vol 98 (5) ◽  
pp. 493-495 ◽  
Author(s):  
Sara Rojas-Dotor ◽  
Guadalupe Rico ◽  
Julia Pérez ◽  
Juan Velázquez ◽  
Raúl Silva ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Cytokine Expression ◽  
Inhibitory Factor ◽  
Cd4 Cells ◽  
Monocyte Locomotion Inhibitory Factor

Isolation and Structural Studies of the Monocyte Locomotion Inhibitory Factor (MLIF) Produced by Entamoeba histolytica

2000 ◽  
Vol 31 (4) ◽  
pp. S76-S77 ◽  
Author(s):  
Roberto Kretschmer ◽  
Guadalupe Rico ◽  
Juan Antonio Giménez
Keyword(s):  
Entamoeba Histolytica ◽  
Inhibitory Factor ◽  
Structural Studies ◽  
Monocyte Locomotion Inhibitory Factor

Chemokines in Leukemic Infiltration of the Central Nervous System in Childhood Acute Lymphoblastic Leukemia.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 1627-1627
Author(s):  
Manuel Ramirez ◽  
Ana M Gomez ◽  
Carolina Martinez ◽  
Alvaro Lassaletta ◽  
Jose L Fuster ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Flow Cytometry ◽  
Nervous System ◽  
Acute Lymphoblastic Leukemia ◽  
Chemokine Receptors ◽  
Lymphoblastic Leukemia ◽  
Cns Disease ◽  
Leukemic Infiltration ◽  
Cns Relapse ◽  
Chemokine Ligands

Abstract Abstract 1627 Poster Board I-653 Background Leukemic blasts from B-cell acute lymphoblastic leukemia (B-ALL) and T-ALL circulate through the blood stream and may infiltrate different organs. Extramedullary organs may act as sanctuaries for lymphoblasts, preventing the exposure to adequate levels of chemotherapeutic drugs. Typical extramedullary relapses are seen in testes and in central nervous system (CNS). We aimed at determining whether chemokines may play a role in the infiltration of the CNS by leukemic blasts in childhood ALL. We studied the expression of chemokine receptors in ALL blasts in marrow, as well as the levels of chemokine ligands in the cerebrospinal fluid (CSF) of children with B- or T-ALL. If chemokines had a role in CNS leukemic infiltration, the following should be confirmed: leukemic blasts should express high levels of the chemokine receptor/s for which high levels of its corresponding ligand/s were detected at the CNS. Methods This prospective study was approved by the local ethical committees for clinical research. Samples from 80 children in 10 Spanish pediatric oncology units were obtained. We detected the presence of leukemic blasts in CFS by flow cytometry. We defined leukemic infiltration of CFS samples as the presence of cells with the same immunophenotype as the leukemia in the marrow aspirates. We detected the expression levels of 9 CCR and 6 CXCR molecules in ALL blasts by flow cytometry in marrow aspirates. Levels of chemokine ligands were quantitated by Cytometric Bead Array or by commercial ELISA kits in CSF samples. Results We found that chemokine receptors expression and levels of chemokine ligands varied depending upon the lineage (T versus B), the maturation state (pre-T versus T; pro-B versus pre-B versus common-B) and the risk-status (high versus non-high) of the leukemia. T-ALL patients with high levels of CNS leukemic infiltration expressed significantly higher levels of CXCL10 compared to the same parameters of T-ALL patients with low/absent levels of CNS disease (p=0,049). Common B-ALL patients with high levels of CNS leukemic infiltration expressed higher levels of CCL22 compared to that of common B-ALL patients with low/absent levels of CNS disease (p=0,059). Among the 4 patients with a CNS relapse, we detected higher levels of CXCR3 (p=0,0038) and of its ligand, CXCL10 (p=0,0169), compared to patients who did not relapse. Conclusions Our study suggests that the CXCR3/CXCL10 axis may be involved in the CNS relapse of high-risk ALL in children: high expression of CXCR3 on marrow blasts plus high levels of CXCL10 in the CNS was associated with leukemic CNS relapse. Disclosures No relevant conflicts of interest to declare.

The Cys-Asn-Ser carboxyl-terminal end group is the pharmacophore of the amebic anti-inflammatory monocyte locomotion inhibitory factor (MLIF)

2008 ◽  
Vol 158 (1) ◽  
pp. 46-51 ◽  
Author(s):  
María Esther Morales-Martínez ◽  
Raúl Silva-García ◽  
Catalina Soriano-Correa ◽  
Juan Antonio Giménez-Scherer ◽  
Sara Rojas-Dotor ◽  
...  
Keyword(s):  
Inhibitory Factor ◽  
Inflammatory Monocyte ◽  
Carboxyl Terminal ◽  
Anti Inflammatory ◽  
Monocyte Locomotion Inhibitory Factor ◽  
End Group

Further characterization of a human monocyte locomotion inhibitory factor produced by axenically grownEntamoeba histolytica

1989 ◽  
Vol 75 (3) ◽  
pp. 245-246 ◽  
Author(s):  
R. R. Kretschmer ◽  
E. M. Castro ◽  
G. Rico ◽  
G. Pacheco ◽  
R. Noriega ◽  
...  
Keyword(s):  
Human Monocyte ◽  
Inhibitory Factor ◽  
Monocyte Locomotion Inhibitory Factor
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