scholarly journals Molecular Markers and Marker-Assisted Breeding in Plants

10.5772/52583 ◽  
2013 ◽  
Author(s):  
Guo-Liang Jiang
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Breeding

scholarly journals Inheritance Pattern and Molecular Markers for Resistance to Blackleg Disease in Cabbage

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 583
Author(s):  
Mostari Jahan Ferdous ◽  
Mohammad Rashed Hossain ◽  
Jong-In Park ◽  
Arif Hasan Khan Robin ◽  
Denison Michael Immanuel Jesse ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Resolution Melting ◽  
Leptosphaeria Maculans ◽  
Inbred Lines ◽  
Indel Marker ◽  
Detection Accuracy ◽  
Nucleotide Polymorphisms ◽  
Blackleg Disease ◽  
Marker Assisted Breeding ◽  
Single Nucleotide

The inheritance and causal loci for resistance to blackleg, a devastating disease of Brassicaceous crops, are yet to be known in cabbage (Brassica oleracea L.). Here, we report the pattern of inheritance and linked molecular marker for this trait. A segregating BC1 population consisting of 253 plants was raised from resistant and susceptible parents, L29 (♀) and L16 (♂), respectively. Cotyledon resistance bioassay of BC1 population, measured based on a scale of 0–9 at 12 days after inoculation with Leptosphaeria maculans isolate 03–02 s, revealed the segregation of resistance and ratio, indicative of dominant monogenic control of the trait. Investigation of potential polymorphism in the previously identified differentially expressed genes within the collinear region of ‘B. napus blackleg resistant loci Rlm1′ in B. oleracea identified two insertion/deletion (InDel) mutations in the intron and numerous single nucleotide polymorphisms (SNPs) throughout the LRR-RLK gene Bol040029, of which six SNPs in the first exon caused the loss of two LRR domains in the susceptible line. An InDel marker, BLR-C-InDel based on the InDel mutations, and a high resolution melting (HRM) marker, BLR-C-2808 based on the SNP C2808T in the second exon were developed, which predicated the resistance status of the BC1 population with 80.24%, and of 24 commercial inbred lines with 100% detection accuracy. This is the first report of inheritance and molecular markers linked with blackleg resistance in cabbage. This study will enhance our understanding of the trait, and will be helpful in marker assisted breeding aiming at developing resistant cabbage varieties.

scholarly journals Characterization of Checo/F95-927 pea (Pisum sativum L.) population for winter frost tolerance using molecular markers

2021 ◽  
Vol 26 (1) ◽  
pp. 2262-2268
Author(s):  
ANCUȚA CRÎNGAȘU (BĂRBIERU) ◽  
MATILDA CIUCĂ ◽  
CRISTINA DANIEL ◽  
CĂLINA-PETRUȚA CORNEA ◽  
GHEORGHE ITTU ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Essential Role ◽  
Field Experiments ◽  
Recombinant Inbred Lines ◽  
Frost Tolerance ◽  
Inbred Lines ◽  
Marker Assisted Breeding ◽  
Pisum Sativum L

Winter pea has several advantages on spring pea: higher yield, more rapid spring growth that helps this crop to compete weeds, wider growing area, etc. The aim of this paper was to evaluate the association of some molecular markers with frost tolerance of pea. Forty-one pea recombinant inbred lines (RILs), from F5 generation, obtained by crossing the winter cultivar Checo with the spring line F95-927 were examined during this work both by in vitro (molecular markers analysis) and in field experiments. The results obtained showed a significant correlation betweeen frost tolerance and molecular markers EST1109 and AD59. Also, it has been found that the molecular marker AD159 is associated with earliness character. These associated markers could play an essential role in marker-assisted breeding for winter-hardy pea cultivars.

scholarly journals Development of diagnostic molecular markers for marker-assisted breeding against bacterial wilt in tomato

2020 ◽  
Vol 70 (4) ◽  
pp. 462-473
Author(s):  
Alebel Mekuriaw Abebe ◽  
Jinwoo Choi ◽  
Youngjun Kim ◽  
Chang-Sik Oh ◽  
Inhwa Yeam ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Bacterial Wilt ◽  
Marker Assisted Breeding

scholarly journals Molecular marker validation and identification of Fusarium wilt resistant chickpea genotypes

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
Vinod Kumar Sahu ◽  
Sushma Tiwari ◽  
M. K. Tripathi ◽  
Neha Gupta ◽  
R. S. Tomar ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Wilt ◽  
Polymorphic Information Content ◽  
Marker Assisted Breeding ◽  
Biochemical Traits ◽  
Significance Level ◽  
Resistant Varieties ◽  
Gene Based Markers ◽  
Physiological And Biochemical ◽  
Number Of Branches

Fusarium wilt is one of the most destructive biotic stress reducing chickpea productivity worldwide. As a step towards understanding the basis of wilt resistance in chickpea, we investigated the morpho-physiological and biochemical traits of 29 desi and 15 kabuli chickpea genotypes and screened these genotypes for fusarium wilt disease using gene based molecular markers. The number of branches per plant were significant and positively correlated with number of pods per plant (r=0.635) and number of seed per plant (r=0.556) at 1% significance level. Biochemical parameters related to stresses were also analyzed for proline (1.19-3.92 µ mol/g), sugar (23.6-37.4 mg/g), malondialdehyde (MDA) (1.1 -3.67 nmol/g) and hydrogen peroxide (H2O2) (10.4-21.5 µ mol/g) in seeds of these genotypes grown under normal field conditions. Molecular screening was done by using15 gene-based markers. Polymorphic Information Content (PIC) value was in the range of 0.221 to 0.695, respectively. The genotypes JG-63 and Vijay identified during the investigation could be included in the hybridization programs during development of high yielding and wilt resistant varieties. The molecular markers TA194, TA-59, TA-96, TR-19, TR-29 and TR-31 can be used as marker assisted breeding tools for screening, validation and development of fusarium wilt resistant chickpea genotypes.

scholarly journals Role of Molecular Markers in Crop Breeding: A Review

2021 ◽  
Author(s):  
Rajitha Jayakumar Nair ◽  
Manoj Kumar Pandey
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Marker Assisted Selection ◽  
Abiotic Stress Tolerance ◽  
Specific Gene ◽  
Biotic And Abiotic Stress ◽  
Marker Assisted Breeding ◽  
Crop Breeding ◽  
Entire Genome

Molecular markers are effective tools used to ‘flag’ the location of a specific gene or the inheritance of a definite trait. Markers are unique DNA fragments that can be identified inside the entire genome. The development of molecular markers combined with high throughput technologies have paved the way for achieving the desirable traits as well as induced biotic and abiotic stress tolerance in plant, which enhanced the crop breeding. Highly polymorphic molecular markers are developed for gene mapping, estimation of genetic diversity, finding out the evolution and phylogeny of crop, analysis of heterosis, assessment of diploid/haploid crops and genotyping of cultivars along with Marker Assisted Breeding (MAB)/Marker Assisted Selection (MAS). These are the most significant objectives for crop breeding. This review reveals about the role of various recently developed molecular markers in the improvement of crop. Molecular markers act as a “milestone” for the researchers who aim to enhance crop breeding.

Simple sequence repeat (SSR) markers linked to E1, E3, E4, and E7 maturity genes in soybean

Genome ◽  
2003 ◽  
Vol 46 (6) ◽  
pp. 1024-1036 ◽  
Author(s):  
Stephen J Molnar ◽  
Satish Rai ◽  
Martin Charette ◽  
Elroy R Cober
Keyword(s):  
Molecular Markers ◽  
Reproductive Period ◽  
Soybean Genome ◽  
Isogenic Line ◽  
Near Isogenic Lines ◽  
Marker Assisted Breeding ◽  
Trait Locus ◽  
First Time ◽  
Map Location ◽  
Simple Sequence

Soybean near isogenic lines (NILs), contrasting for maturity and photoperiod sensitivity loci, were genotyped with approximately 430 mapped simple sequence repeats (SSRs), also known as microsatellite markers. By analysis of allele distributions across the NILs, it was possible to confirm the map location of the Dt1 indeterminate growth locus, to refine the SSR mapping of the T tawny pubescence locus, to map E1 and E3 maturity loci with molecular markers, and to map the E4 and E7 maturity loci for the first time. Molecular markers flanking these loci are now available for marker-assisted breeding for these traits. Analysis of map locations identified a putative homologous relationship among four chromosomal regions; one in the middle of linkage group (LG) C2 carrying E1 and E7, one on LG I carrying E4, one at the top of LG C2, at which there is a reproductive period quantitative trait locus (QTL), and the fourth on LG B1. Other evidence suggests that homology also exists between the E1 + E7 region on LG C2 and a region on LG L linked to a pod maturity QTL. Homology relationships predict possible locations in the soybean genome of additional maturity loci, as well as which maturity loci may share a common evolutionary origin and similar mechanism(s) of action.Key words: soybean, maturity, photoperiod, microsatellite marker, near isogenic line.

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