scholarly journals Molecular marker validation and identification of Fusarium wilt resistant chickpea genotypes

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
Vinod Kumar Sahu ◽  
Sushma Tiwari ◽  
M. K. Tripathi ◽  
Neha Gupta ◽  
R. S. Tomar ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Fusarium Wilt ◽  
Polymorphic Information Content ◽  
Marker Assisted Breeding ◽  
Biochemical Traits ◽  
Significance Level ◽  
Resistant Varieties ◽  
Gene Based Markers ◽  
Physiological And Biochemical ◽  
Number Of Branches

Fusarium wilt is one of the most destructive biotic stress reducing chickpea productivity worldwide. As a step towards understanding the basis of wilt resistance in chickpea, we investigated the morpho-physiological and biochemical traits of 29 desi and 15 kabuli chickpea genotypes and screened these genotypes for fusarium wilt disease using gene based molecular markers. The number of branches per plant were significant and positively correlated with number of pods per plant (r=0.635) and number of seed per plant (r=0.556) at 1% significance level. Biochemical parameters related to stresses were also analyzed for proline (1.19-3.92 µ mol/g), sugar (23.6-37.4 mg/g), malondialdehyde (MDA) (1.1 -3.67 nmol/g) and hydrogen peroxide (H2O2) (10.4-21.5 µ mol/g) in seeds of these genotypes grown under normal field conditions. Molecular screening was done by using15 gene-based markers. Polymorphic Information Content (PIC) value was in the range of 0.221 to 0.695, respectively. The genotypes JG-63 and Vijay identified during the investigation could be included in the hybridization programs during development of high yielding and wilt resistant varieties. The molecular markers TA194, TA-59, TA-96, TR-19, TR-29 and TR-31 can be used as marker assisted breeding tools for screening, validation and development of fusarium wilt resistant chickpea genotypes.

scholarly journals Using SSR Markers For Assessment Genetic Diversity And Detection Drought Escape Candidate Genes In Barley Lines (Hordeum Vulgare L.)

2014 ◽  
Vol 70 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Vahideh Gougerdchi ◽  
Sara Dezhsetan ◽  
Mohammad Ali Ebrahimi ◽  
Behzad Sadeghzadeh ◽  
Sona Savari
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Molecular Markers ◽  
Candidate Genes ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Marker Assisted Breeding ◽  
Hordeum Vulgare L ◽  
Breeding Programs ◽  
Mapping Analysis

Abstract Assessment of genetic diversity using molecular markers is one of the primary and important steps in breeding programs. In this study, genetic diversity of 52 barley lines evaluated using 68 SSR primer pairs and 47 primer pairs produced clear and polymorphic banding pattern. In general, 153 polymorphic alleles detected. The number of observed polymorphic alleles varied from 2 to 9, with an average of 3.26 alleles per locus. Polymorphic Information Content (PIC) ranged from 0.07 to 0.81, with an average of 0.45. In this research, SSR markers differentiated the studied lines efficiently. Using cluster analysis, studied barley lines divided into two groups. Genetic diversity was relatively corresponding with geographical origins, because the lines related to a country somewhat diverged from each other. Two-rowed Iranian and Chinese barleys classified in one subgroup. Also, most six-rowed barleys classified in one subgroup. Association mapping analysis was used to identify candidate genes for drought escape in barley lines and 16 informative markers were identified after which confirmation in other tests could be suitable for marker assisted breeding drought escape.

Stevia rebaudiana (Bert.) Bertoni cultivated under different photoperiod conditions: Improving physiological and biochemical traits for industrial applications

2021 ◽  
Vol 168 ◽  
pp. 113595
Author(s):  
Marcos Vinicius Silva de Andrade ◽  
Renato Delmondez de Castro ◽  
Diego da Silva Cunha ◽  
Valdir Gomes Neto ◽  
Maria Gabriela Aparecida Carosio ◽  
...  
Keyword(s):  
Stevia Rebaudiana ◽  
Industrial Applications ◽  
Biochemical Traits ◽  
Physiological And Biochemical

Effect of marennine produced by the blue diatom Haslea ostrearia on behavioral, physiological and biochemical traits of juvenile Mytilus edulis and Crassostrea virginica

Aquaculture ◽  
2017 ◽  
Vol 467 ◽  
pp. 138-148 ◽  
Author(s):  
Fiddy S. Prasetiya ◽  
Luc A. Comeau ◽  
Romain Gastineau ◽  
Priscilla Decottignies ◽  
Bruno Cognie ◽  
...  
Keyword(s):  
Mytilus Edulis ◽  
Crassostrea Virginica ◽  
Biochemical Traits ◽  
Haslea Ostrearia ◽  
Physiological And Biochemical

scholarly journals Physiological and biochemical traits of sesame (Sesamum indicum L.) varieties under rainfed conditions

2020 ◽  
Vol 8 (5) ◽  
pp. 2277-2281
Author(s):  
KN Ravitej ◽  
P Ratna Kumar ◽  
S Narender Reddy ◽  
Praduman Yadav V ◽  
Gouri Shankar ◽  
...  
Keyword(s):  
Sesamum Indicum ◽  
Biochemical Traits ◽  
Rainfed Conditions ◽  
Physiological And Biochemical

scholarly journals Effect of Heat Stress on Inter-relationship of Physiological and Biochemical Traits with Grain Yield in Wheat (Triticum aestivum L.)

2020 ◽  
pp. 19-29
Author(s):  
Amrita Kumari ◽  
R. D. Ranjan ◽  
Chandan Roy ◽  
Awadesh Kumar Pal ◽  
S. Kumar
Keyword(s):  
Heat Stress ◽  
Grain Yield ◽  
Flag Leaf ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Seedling Stage ◽  
Biochemical Traits ◽  
Highly Correlated ◽  
Complex Phenomena ◽  
Physiological And Biochemical

Heat stress, particularly the stress appears at the time of flowering to grain filling stages causing severe yield loss in wheat. Heat tolerance is complex phenomena that include adjustment in morphological, physiological and biochemical traits of the crop. Present investigation was carried out to understand the effect of terminal heat stress on different traits of wheat. The experiment was conducted in three dates of sowing as timely sown, late sown and very late sown to expose the crop to heat stress at later stages of the crop growth. Significant genetic variations for all the traits evaluated under three conditions indicated the presence of variability for the traits. Trait association analysis revealed that flag leaf chlorophyll content and MSI at seedling stage; MDA at reproductive stage had direct relationship with grain yield. While under very late sown condition MDA and RWC at seedling stages were found to be highly correlated with grain yield. It indicates that MDA, RWC at seedling stage and days to booting, days to milking plays important role in very late sown condition that can be used as selection criteria in breeding programme.

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