scholarly journals RNA 5′-end Maturation: A Crucial Step in the Replication of Viral Genomes

10.5772/56166 ◽  
2013 ◽  
Author(s):  
Frdric Picard-Jean ◽  
Maude Tremblay-Ltourneau ◽  
Elizabeth Serra ◽  
Christina Dimech ◽  
Helene Schulz ◽  
...  
Keyword(s):  
Crucial Step ◽  
Viral Genomes

Letter Verbal Fluency in Spanish-, Basque-, and Catalan-Speaking Individuals: Does the Selection of the Letters Influence the Outcome?

2019 ◽  
Vol 62 (7) ◽  
pp. 2400-2410
Author(s):  
Laiene Olabarrieta-Landa ◽  
Itziar Benito-Sánchez ◽  
Montserrat Alegret ◽  
Anna Gailhajanet ◽  
Esther Landa Torre ◽  
...  
Keyword(s):  
Frontal Lobe ◽  
Verbal Fluency ◽  
Native Language ◽  
Spanish Speakers ◽  
Crucial Step ◽  
Verbal Fluency Test ◽  
Performance Differences ◽  
Frontal Lobe Functioning ◽  
Selection Of ◽  
Fluency Test

Purpose The aim of this study was to compare Basque and Catalan bilinguals' performance on the letter verbal fluency test and determine whether significant differences are present depending on the letters used and the language of administration. Method The sample consisted of 87 Spanish monolinguals, 139 Basque bilinguals, and 130 Catalan bilinguals from Spain. Participants completed the letter verbal fluency test using the letters F, A, S, M, R, P, and E. Results Bilinguals scored higher on the letter verbal fluency test when they were tested in Spanish than in Basque or Catalan. No performance differences were found according to native language or dialects within Basque participants. Catalans with Spanish as their native language scored lower on the letter F compared to those who grew up speaking Catalan and Spanish. The suggested letters to use with Basque speakers are A, E, and B; the suggested letters to use with Catalan speakers are P, F, and M; and the suggested letters to use with Spanish speakers are M, R, and P. Conclusion Selecting appropriate stimuli depending on the language of testing is the first crucial step to assess verbal fluency and thus possible frontal lobe functioning impairment.

How Dialogical Are We Really? Insights Gleaned from Un-Dialogical Moments

2017 ◽  
Vol 6 (4) ◽  
pp. 47-69 ◽  
Author(s):  
Dongjing Kang ◽  
Chigozirim Utah Sodeke
Keyword(s):  
Mikhail Bakhtin ◽  
Research Process ◽  
Martin Buber ◽  
Crucial Step ◽  
Research Reporting

This essay emphasizes the writing of dialogical research as a crucial step in the dialogical research process. Dialogical research accounts should not suppress the ongoing struggles that accompany a genuine desire to engage dialogically in research contexts. Thus, we advocate and model evocative retellings of these struggles. Questioning our own fieldwork based on the work of Martin Buber and Mikhail Bakhtin, we highlight principles of dialogue that also serve as guidelines for dialogical research reporting: unfinalizability, engaging paradoxes, and creative (critical) transformation.

Annotating Viral Genomes - A Cannon is Needed to Kill Mosquitoes

2014 ◽  
Vol 9 (2) ◽  
pp. 132-139
Author(s):  
Shiliang Wang
Keyword(s):  
Viral Genomes

Method for determining the extent and copy number of overlapping and nonoverlapping segments of integrated viral genomes.

1979 ◽  
Vol 31 (2) ◽  
pp. 575-578 ◽  
Author(s):  
C T Patch ◽  
J Hauser ◽  
A M Lewis ◽  
A S Levine
Keyword(s):  
Copy Number ◽  
Viral Genomes

Enzymes of Human Herpesviruses

1992 ◽  
Vol 57 (8) ◽  
pp. 1577-1612 ◽  
Author(s):  
Pavel Kramata ◽  
Ivan Votruba
Keyword(s):  
Binding Protein ◽  
Human Herpesvirus ◽  
Dna Helicase ◽  
Point Of View ◽  
Uracil Dna Glycosylase ◽  
Replication Complex ◽  
Viral Genomes ◽  
Origin Binding Protein ◽  
Antiviral Chemotherapy ◽  
Human Herpesviruses

The properties of human herpesvirus-encoded enzymes are reviewed and the importance of sequence analysis of viral genomes as well as the experiments on characteristics of enzymes isolated from infected cell cultures are emphasized. The following enzymes are described in detail: DNA replication complex consisting of DNA polymerase, DNA helicase-primase, single-stranded DNA binding protein and origin binding protein, further thymidine kinase, ribonucleotide reductase, deoxyuridine triphosphatase as well as uracil-DNA-glycosylase, deoxyribonuclease and protein kinase. The importance of these enzymes from the point of view of antiviral chemotherapy is discussed.

scholarly journals Latest Advances of Virology Research Using CRISPR/Cas9-Based Gene-Editing Technology and Its Application to Vaccine Development

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 779
Author(s):  
Man Teng ◽  
Yongxiu Yao ◽  
Venugopal Nair ◽  
Jun Luo
Keyword(s):  
Biological Sciences ◽  
Gene Therapy ◽  
Genetic Engineering ◽  
Gene Function ◽  
Viral Genome ◽  
Gene Editing ◽  
Vaccine Development ◽  
Future Prospects ◽  
Dna Viruses ◽  
Viral Genomes

In recent years, the CRISPR/Cas9-based gene-editing techniques have been well developed and applied widely in several aspects of research in the biological sciences, in many species, including humans, animals, plants, and even in viruses. Modification of the viral genome is crucial for revealing gene function, virus pathogenesis, gene therapy, genetic engineering, and vaccine development. Herein, we have provided a brief review of the different technologies for the modification of the viral genomes. Particularly, we have focused on the recently developed CRISPR/Cas9-based gene-editing system, detailing its origin, functional principles, and touching on its latest achievements in virology research and applications in vaccine development, especially in large DNA viruses of humans and animals. Future prospects of CRISPR/Cas9-based gene-editing technology in virology research, including the potential shortcomings, are also discussed.

Rhetoric beyond Arguments: Thinking about the Role of Fictional Audiences in Plato’s Gorgias

Elenchos ◽  
2020 ◽  
Vol 41 (2) ◽  
pp. 217-243
Author(s):  
Dora Suarez
Keyword(s):  
Crucial Step ◽  
Universal Audience ◽  
Seminal Work ◽  
New Rhetoric ◽  
Chaim Perelman ◽  
The Way

AbstractIn this piece, I propose a reading of Plato’s Gorgias that pays special attention to the role that the fictional audience plays in the unfolding of the dialogue. To this end, I use some of the insights that Chaïm Perelman and Lucie Olbrechts–Tyteca conveyed in their seminal work, The New Rhetoric: A Treatise on Argumentation in order to argue that thinking about the way in which Socrates’ arguments are shaped by the different audiences that Gorgias, Polus, and Callicles aim to address and represent provides us with a new hermeneutical understanding of what is at stake in each of the different interactions Socrates engages in throughout the dialogue. In unpacking the way in which Socrates appropriates Gorgias’ particular audience, transforms Polus’ universal audience, and challenges Callicles’ elite audience, I provide an outline of the difficulties that Plato’s Socrates has to overcome in order to achieve the ‘community of minds’ that Perelman and Olbrechts–Tyteca identify as the bedrock of fruitful argumentation. Having done this, in the last section I turn to Plato’s Phaedrus, for the purpose of making evident that thinking about Plato’s deployment of rhetorical audiences is a crucial step in the effort to expose the implicit continuity that links the discussion of rhetoric delivered by the Gorgias to that of the Phaedrus.

scholarly journals Persistent Human Papillomavirus Infection

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 321
Author(s):  
Ashley N. Della Fera ◽  
Alix Warburton ◽  
Tami L. Coursey ◽  
Simran Khurana ◽  
Alison A. McBride
Keyword(s):  
Human Papillomavirus ◽  
Dna Replication ◽  
Cellular Proliferation ◽  
Basal Cells ◽  
Viral Dna ◽  
Viral Dna Replication ◽  
Viral Genomes ◽  
Cellular Environment ◽  
E6 And E7 ◽  
Cellular Replication

Persistent infection with oncogenic human papillomavirus (HPV) types is responsible for ~5% of human cancers. The HPV infectious cycle can sustain long-term infection in stratified epithelia because viral DNA is maintained as low copy number extrachromosomal plasmids in the dividing basal cells of a lesion, while progeny viral genomes are amplified to large numbers in differentiated superficial cells. The viral E1 and E2 proteins initiate viral DNA replication and maintain and partition viral genomes, in concert with the cellular replication machinery. Additionally, the E5, E6, and E7 proteins are required to evade host immune responses and to produce a cellular environment that supports viral DNA replication. An unfortunate consequence of the manipulation of cellular proliferation and differentiation is that cells become at high risk for carcinogenesis.

Sign in / Sign up

Export Citation Format

Share Document