Vitamin B1 (Thiamine) Metabolism and Regulation in Archaea

It is well established that thiamine deficiency results in an excess of metabolic intermediates such as lactate and pyruvate, which is likely due to insufficient levels of cofactor for the function of thiamine-dependent enzymes. When in excess, both pyruvate and lactate can increase the stabilization of the hypoxia-inducible factor 1-alpha (HIF-1α) transcription factor, resulting in the trans-activation of HIF-1α regulated genes independent of low oxygen, termed pseudo-hypoxia. Therefore, the resulting dysfunction in cellular metabolism and accumulation of pyruvate and lactate during thiamine deficiency may facilitate a pseudo-hypoxic state. In order to investigate the possibility of a transcriptional relationship between hypoxia and thiamine deficiency, we measured alterations in metabolic intermediates, HIF-1α stabilization, and gene expression. We found an increase in intracellular pyruvate and extracellular lactate levels after thiamine deficiency exposure to the neuroblastoma cell line SK-N-BE. Similar to cells exposed to hypoxia, there was a corresponding increase in HIF-1α stabilization and activation of target gene expression during thiamine deficiency, including glucose transporter-1 (GLUT1), vascular endothelial growth factor (VEGF), and aldolase A. Both hypoxia and thiamine deficiency exposure resulted in an increase in the expression of the thiamine transporter SLC19A3. These results indicate thiamine deficiency induces HIF-1α-mediated gene expression similar to that observed in hypoxic stress, and may provide evidence for a central transcriptional response associated with the clinical manifestations of thiamine deficiency.

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PENGARUH KOMBINASI INTENSITAS NAUNGAN DENGAN ZAT PENGATUR TUMBUH INDOLE BUTIRIC ACID (IBA), NAPHTHALENE ACETIC ACID (NAA), DAN VITAMIN B1 DALAM AKLIMATISASI PERTUMBUHAN BIBIT GAHARU (Aquilaria beccariana)

Jurnal Sains dan Teknologi Indonesia ◽

10.29122/jsti.v14i3.922 ◽

2013 ◽

Vol 14 (3) ◽

Author(s):

Daru Mulyono

Keyword(s):

Acetic Acid ◽

Factorial Design ◽

Vitamin B1 ◽

Naphthalene Acetic Acid ◽

Randomized Design ◽

Optimal Formula

The objective of this research is to know the optimal formula of Indole Butiric Acid (IBA), Naphthalene Acetic Acid (NAA), Vitamine B1 and the combination with shading intensities to the acclimatization of Gaharu stump (Aquilaria beccariana). This research used Factorial Design with basic analysis of Complete Randomized Design in order to know theeffect of treatment. The research was carried out in Agroindustry and Biotechnology Laboratory, Ciampea, Bogor, from July to September 2007. The results of the research showed that after 8 weeks of treatment: (a). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 10 mg/l + Vitamine B1 1 mg/l was the best formula for increasingheight of Gaharu stump 4.660 cm. (b). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 30 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sum of Gaharu leaf stump 12.337 leafs, (c). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing sumof Gaharu root stump 3.783 roots, and (d). The combination of 55 % shading intensity with IBA 15 mg/l + NAA 40 mg/l + Vitamine B1 1 mg/l was the best formula for increasing length of Gaharu root stump 3.686 cm.

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Comparison of Extraction Methods for the Determination of Vitamin B1 and B3 in Foods

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2020.49.8.822 ◽

2020 ◽

Vol 49 (8) ◽

pp. 822-828

Author(s):

Jimin Yoon ◽

Naeun Kim ◽

Ahyeong Jeon ◽

Jihyun Kwon ◽

Sang-Hoon Lee ◽

...

Keyword(s):

Vitamin B1 ◽

Extraction Methods

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Vitamin B1: A Versatile Organocatalyst for Organic Synthesis

Current Organocatalysis ◽

10.2174/2213337204666170824163521 ◽

2017 ◽

Vol 4 (2) ◽

Author(s):

Ruby Singh ◽

Shakeel Ahmad Ganaie ◽

Aakash Singh

Keyword(s):

Organic Synthesis ◽

Vitamin B1

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A novel PET tracer 18F-deoxy-thiamine: synthesis, metabolic kinetics, and evaluation on cerebral thiamine metabolism status

EJNMMI Research ◽

10.1186/s13550-020-00710-5 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Changpeng Wang ◽

Siwei Zhang ◽

Yuefei Zou ◽

Hongzhao Ma ◽

Donglang Jiang ◽

...

Keyword(s):

High Performance ◽

Specific Activity ◽

High Accumulation ◽

Metabolic Kinetics ◽

Thiamine Metabolism ◽

Pet Tracer ◽

The Status ◽

The Brain

Abstract Background Some neuropsychological diseases are associated with abnormal thiamine metabolism, including Korsakoff–Wernicke syndrome and Alzheimer’s disease. However, in vivo detection of the status of brain thiamine metabolism is still unavailable and needs to be developed. Methods A novel PET tracer of 18F-deoxy-thiamine was synthesized using an automated module via a two-step route. The main quality control parameters, such as specific activity and radiochemical purity, were evaluated by high-performance liquid chromatography (HPLC). Radiochemical concentration was determined by radioactivity calibrator. Metabolic kinetics and the level of 18F-deoxy-thiamine in brains of mice and marmosets were studied by micro-positron emission tomography/computed tomography (PET/CT). In vivo stability, renal excretion rate, and biodistribution of 18F-deoxy-thiamine in the mice were assayed using HPLC and γ-counter, respectively. Also, the correlation between the retention of cerebral 18F-deoxy-thiamine in 60 min after injection as represented by the area under the curve (AUC) and blood thiamine levels was investigated. Results The 18F-deoxy-thiamine was stable both in vitro and in vivo. The uptake and clearance of 18F-deoxy-thiamine were quick in the mice. It reached the max standard uptake value (SUVmax) of 4.61 ± 0.53 in the liver within 1 min, 18.67 ± 7.04 in the kidney within half a minute. The SUV dropped to 0.72 ± 0.05 and 0.77 ± 0.35 after 60 min of injection in the liver and kidney, respectively. After injection, kidney, liver, and pancreas exhibited high accumulation level of 18F-deoxy-thiamine, while brain, muscle, fat, and gonad showed low accumulation concentration, consistent with previous reports on thiamine distribution in mice. Within 90 min after injection, the level of 18F-deoxy-thiamine in the brain of C57BL/6 mice with thiamine deficiency (TD) was 1.9 times higher than that in control mice, and was 3.1 times higher in ICR mice with TD than that in control mice. The AUC of the tracer in the brain of marmosets within 60 min was 29.33 ± 5.15 and negatively correlated with blood thiamine diphosphate levels (r = − 0.985, p = 0.015). Conclusion The 18F-deoxy-thiamine meets the requirements for ideal PET tracer for in vivo detecting the status of cerebral thiamine metabolism.

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Directing cation-cation interactions in thiamine compounds: Analysis of a series of organic salts based on vitamin B1

Journal of Molecular Structure ◽

10.1016/j.molstruc.2021.130046 ◽

2021 ◽

Vol 1232 ◽

pp. 130046

Author(s):

Joseph Traver ◽

Erica Chenard ◽

Matthias Zeller ◽

Gary L. Guillet ◽

Will E. Lynch ◽

...

Keyword(s):

Vitamin B1 ◽

Organic Salts

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Vitamin Intake and Loss of Muscle Mass in Older People with Type 2 Diabetes: A Prospective Study of the KAMOGAWA-DM Cohort

Nutrients ◽

10.3390/nu13072335 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2335

Author(s):

Fuyuko Takahashi ◽

Yoshitaka Hashimoto ◽

Ayumi Kaji ◽

Ryosuke Sakai ◽

Yuka Kawate ◽

...

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Older People ◽

Vitamin B12 ◽

Muscle Mass ◽

Vitamin B1 ◽

Vitamin D Intake ◽

Glucagon Like Peptide 1

The aim of this prospective cohort study was to examine the relationships between the intakes of various vitamins and the loss of muscle mass in older people with type 2 diabetes (T2DM). The change in skeletal muscle mass index (SMI, kg/m2) (kg/m2/year) was defined as follows: (SMI at baseline (kg/m2) − SMI at follow-up (kg/m2))/follow-up period (year). The rate of SMI reduction (%) was calculated as follows (the change in SMI (kg/m2/year)/SMI at baseline (kg/m2)) × 100. The rate of SMI reduction ≥ 1.2% was considered as the loss of muscle mass. Among 197 people with T2DM, 47.2% of them experienced the loss of muscle mass at the 13.7 ± 5.2 month follow-up. Vitamin B1 (0.8 ± 0.3 vs. 0.8 ± 0.3 mg/day, p = 0.031), vitamin B12 (11.2 ± 8.3 vs. 13.4 ± 7.5 μg/day, p = 0.049), and vitamin D (16.5 ± 12.2 vs. 21.6 ± 13.0 μg/day, p = 0.004) intakes in people with the loss of muscle mass were significantly lower than those without. Vitamin D intake was related to the loss of muscle mass after adjusting for sex, age, exercise, alcohol, smoking, body mass index, SMI, glucagon-like peptide-1 agonist, sodium glucose cotransporter-2 inhibitor, insulin, HbA1c, creatinine, energy intake, and protein intake (adjusted odds ratio 0.93, 95% confidence interval: 0.88–0.97, p = 0.003). This study showed that vitamin D intake was related to the loss of muscle mass in older people with T2DM. Vitamin B12 intake tended to be related to the loss of muscle mass, although vitamin A, vitamin B2, vitamin B6, vitamin C, and vitamin E intake were not related.

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