Interactions of Candida albicans Cells with Aerobic and Anaerobic Bacteria during Formation of Mixed Biofilms in the Oral Cavity

Six preparations (four liquid and two solid) were tested in a double-blind crossover design for their anti-bacterial effect on aerobic and anaerobic bacteria in saliva of 12 volunteers. The four liquid preparations contained either tyrothricin, hexetidine, hydrogen peroxide or ethanol and were tested against a rinse with water as control. The two solid preparations, in the form of lozenges, contained tyrothricin in doses of 4 or 10 mg and were tested against parafilm. A single rinse with either tyrothricin or hexetidine resulted in a significant reduction of aerobic and anaerobic bacteria in saliva which lasted for the whole 1h observation period and was considerably more effective than hydrogen peroxide or ethanol. Similar results were seen with the solid preparations. The lozenge containing 10 mg tyrothricin was the most effective and was the only formulation capable of reducing oral aerobic bacterial counts by a factor of approximately 100.

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Bacteriology of Adenoids and Tonsils in Children with Recurrent Adenotonsillitis

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348940111000908 ◽

2001 ◽

Vol 110 (9) ◽

pp. 844-848 ◽

Cited By ~ 38

Author(s):

Itzhak Brook ◽

Kiran Shah

Keyword(s):

Candida Albicans ◽

Haemophilus Influenzae ◽

Mixed Infection ◽

Anaerobic Bacteria ◽

Recurrent Group ◽

Potential Source ◽

History Of ◽

Group A ◽

Aerobic And Anaerobic

Adenoids and tonsils electively removed from 25 children with a history of recurrent group A β-hemolytic streptococci (GABHS) adenotonsillitis were cultured for aerobic and anaerobic bacteria. Two hundred twenty-four organisms (112 aerobes and facultatives, 110 anaerobes, and 2 Candida albicans) were isolated from the tonsils, and 229 (111 aerobes and facultatives and 118 anaerobes) were isolated from the adenoids. Mixed infection was present in all instances, with an average of 9.1 isolates per specimen. The predominant aerobes were Streptococcus sp, Haemophilus influenzae, and GABHS, and the prevalent anaerobes were Peptostreptococcus, Prevotella, and Fusobacterium spp. β-Lactamase-producing bacteria were detected in 72 isolates recovered from 22 tonsils (88%) and in 74 isolates recovered from 21 adenoids (84%). Discrepancies in the organisms recovered were found between the tonsils and adenoids. Of the aerobic isolates, 20% were isolated only in tonsils and 18% only in adenoids. Of the anaerobes, 20% were found only in tonsils and 26% only in adenoids. This study demonstrates a polymicrobial aerobic-anaerobic flora in both adenoids and tonsils, and discrepancies in recovery of pathogens such as GABHS. The adenoids may serve as a potential source of tonsillitis caused by GABHS.

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Alimentary necrobacillosis in alpacas

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638720906409 ◽

2020 ◽

Vol 32 (2) ◽

pp. 339-343

Author(s):

Francisco R. Carvallo ◽

Francisco A. Uzal ◽

Carlos Flores ◽

Santiago S. Diab ◽

Federico Giannitti ◽

...

Keyword(s):

Oral Cavity ◽

Traumatic Event ◽

Anaerobic Bacteria ◽

Fusobacterium Necrophorum ◽

Postmortem Examination ◽

South American ◽

Autopsy Finding ◽

South American Camelids ◽

Associated Lesions ◽

Aerobic And Anaerobic

Ulcers of the oral cavity, esophagus, and gastric compartments of South American camelids are uncommon. Multifocal-to-coalescing ulcers were identified in the oral cavity, esophagus, and/or gastric compartments of 5 alpacas submitted for postmortem examination. Fusobacterium necrophorum was isolated from the lesions in all alpacas, in combination with other aerobic and anaerobic bacteria. In 4 of these cases, F. necrophorum–associated lesions were considered secondary to neoplasia or other chronic debilitating conditions; in 1 case, the alimentary ulcers were considered the most significant autopsy finding. It is not known if this agent acted as a primary or opportunistic agent in mucosal membranes previously damaged by a traumatic event, chemical insult, immunodeficiency, or any other debilitating condition of the host.

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Paronychia: a Mixed Infection

Journal of Hand Surgery (European Volume) ◽

10.1016/0266-7681(93)90063-l ◽

1993 ◽

Vol 18 (3) ◽

pp. 358-359 ◽

Cited By ~ 26

Author(s):

I. BROOK

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Mixed Infection ◽

Anaerobic Bacteria ◽

Eikenella Corrodens ◽

Bacteroides Species ◽

Group A ◽

Aerobic And Anaerobic ◽

Fusobacterium Species

Cultures of paronychia of the fingers have grown aerobic and anaerobic bacteria. Each was recovered individually in only about a quarter of the patients and a mixed aerobic/anaerobic flora was isolated in half of the patients. The predominant anaerobic organisms were Gram-positive anaerobic cocci, Bacteroides species and Fusobacterium species. The predominant aerobic organisms were Staphylococcus aureus, gamma-haemolytic streptococci, Eikenella corrodens, group A beta-hemolytic streptococci, alpha-hemolytic streptococci, and Klebsiella pneumoniae. Candida albicans was recovered in about 15% of the cases. The management of this infection is discussed.

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Toothbrush: A Favorable Media for Bacterial Growth

International Journal of Experimental Dental Science ◽

10.5005/jp-journals-10029-1035 ◽

2013 ◽

Vol 2 (1) ◽

pp. 27-28 ◽

Cited By ~ 1

Author(s):

Varun Kulkarni

Keyword(s):

Oral Cavity ◽

Bacterial Growth ◽

Oral Hygiene ◽

Anaerobic Bacteria ◽

Agar Plate ◽

Healthy Population ◽

Microbial Analysis ◽

Cooked Meat ◽

Aerobic And Anaerobic ◽

True Alternative

ABSTRACT Introduction In this era of stem cell transplantation, tissue engineering and piezosurgery, it is important to consider the toothbrush as a source of potential pathogens. As toothbrush plays an important everyday role for personal oral hygiene and effective plaque removal. Oral cavity incubates diversity of microorganisms; therefore it is not surprising that some of these microorganisms were transferred to toothbrush during use. Presence of nutrients, epithelial debris and oral secretions make the toothbrush a favorable media for numerous bacterial growths. Aim The main aim of this study was to evaluate the microbial flora predominantly present in toothbrushes. Materials and methods Samples of 150 different used toothbrushes from healthy population were taken randomly for microbial analysis. Samples of toothbrushes were put in Robertson cooked meat (RCM) media for both aerobic and anaerobic microbial recognition. Inoculation was done on blood agar and MacConkey's agar plate and culture plates were incubated for 24 hours. Results Fifty-four samples showed facultative anaerobes growth, i.e. Streptococcus mutans, Alpha-hemolytic streptococci, coagulase negative Staphylococci (CONS) and diphtheroids. Sixty-six samples showed polymicrobial etiology and 30 showed purely anaerobic bacteria, i.e. Bacteroides, Lactobacilli. Conclusion Toothbrush is thus considered a potential biohazard for reintroduction of microorganisms in the oral cavity. New oral hygiene devices like Dental Air Force home dental cleaning system will emerge as true alternative for toothbrush. How to cite this article Saini R, Kulkarni V. Toothbrush: A Favorable Media for Bacterial Growth. Int J Experiment Dent Sci 2013;2(1):27-28.

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Ex vivo comparative evaluation of efficacy of disinfecting ability of garlic oil, neem oil, clove oil and tulsi oil with autoclaving on endodontic K files, tested against aerobic bacteria

UNIVERSITY JOURNAL OF DENTAL SCIENCES ◽

10.21276/ujds.2020.6.3.6 ◽

2021 ◽

Vol 6 (3) ◽

pp. 27-31

Author(s):

Asna Isani ◽

Updesh Masih ◽

Kairavi Joshi

Keyword(s):

Oral Cavity ◽

Gold Standard ◽

Comparative Evaluation ◽

Ex Vivo ◽

Anaerobic Bacteria ◽

Aerobic Bacteria ◽

Clove Oil ◽

Neem Oil ◽

Garlic Oil ◽

Aerobic And Anaerobic

Aim: This study aims for comparative evaluation of efficacy of disinfecting ability of garlic oil, neem oil, clove oil and tulsi oil with autoclaving on endodontic k files tested against aerobic and anaerobic bacteria. Materials and Methods: 50 endodontic K-file were used in oral cavity as sample size, which were immersed into 4 different herbal oils and were checked for its disinfecting ability with three different methods. Results: In this study garlic oil is more effective against aerobic bacteria when compared to neem oil, clove oil and tulsi oil. Conclusion: Garlic oil can be used as an alternative method to autoclave against aerobic bacteria but autoclave is a gold standard disinfecting method for sterilization.

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Surface microhardness of different restorative materials exposed to candida albicans biofilm isolated from the oral cavity of hiv-infected children

Rio de Janeiro Dental Journal (Revista Científica do CRO-RJ) ◽

10.29327/24816.4.1-14 ◽

2019 ◽

Vol 4 (1) ◽

pp. 87-91

Author(s):

Leandro COSTA ◽

Larissa SOARES-SILVA ◽

Paulini Malfei De C. COSTA ◽

Adrielle MANGABEIRA ◽

Maristela PORTELA ◽

...

Keyword(s):

Candida Albicans ◽

Oral Cavity ◽

Surface Microhardness ◽

Restorative Materials

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Aerobic and Anaerobic Bacteria in the Middle ear and ear Canal in Acute Otitis Media

Acta Oto-Laryngologica ◽

10.3109/00016488209108484 ◽

1982 ◽

Vol 93 (sup386) ◽

pp. 100-102 ◽

Cited By ~ 3

Author(s):

J. Luotonen ◽

A. M. M. Jokipii ◽

P. Sipilä ◽

J. Väyrynen ◽

L. Jokipii ◽

...

Keyword(s):

Otitis Media ◽

Middle Ear ◽

Anaerobic Bacteria ◽

Acute Otitis Media ◽

Ear Canal ◽

Aerobic And Anaerobic

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Clonal similarity of salivary and nasopharyngeal Fusobacterium nucleatum in infants with acute otitis media experience

Journal of Medical Microbiology ◽

10.1099/jmm.0.05441-0 ◽

2004 ◽

Vol 53 (2) ◽

pp. 161-165 ◽

Cited By ~ 12

Author(s):

Gunnsteinn Haraldsson ◽

W. Peter Holbrook ◽

Eija Könönen

Keyword(s):

Oral Cavity ◽

Otitis Media ◽

Anaerobic Bacteria ◽

Acute Otitis Media ◽

Fusobacterium Nucleatum ◽

Potential Source ◽

History Of ◽

Arbitrarily Primed Pcr ◽

Inoculation Route ◽

Media Experience

The environment of an infant's nasopharynx during acute otitis media (AOM) favours the growth of anaerobic bacteria, which can be recovered frequently during infection, but hardly at all if the infant is healthy. The aim of this investigation was to identify the potential source and inoculation route of anaerobes that were present in the nasopharynx. Eleven Fusobacterium nucleatum isolates that were collected through the nasal cavity from the nasopharynx of eight infants with a history of AOM, and 161 F. nucleatum isolates from the saliva of the same infants, were typed to the clonal level by using arbitrarily primed PCR (AP-PCR). In five of the eight infants examined, identical AP-PCR types were found among nasopharyngeal and salivary isolates. As anaerobes seem to be present only transiently in the nasopharynx and salivary contamination of the nasopharyngeal samples can be excluded, this observation indicates that the source of nasopharyngeal anaerobes is the oral cavity and that saliva is their transmission vehicle.

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