scholarly journals Detection of Benzo[a]Pyrene Diol Epoxide-DNA Adducts in White Blood Cells of Asphalt Plant Workers in Syria

2021 ◽  
Author(s):  
Razan Zohairee
Keyword(s):  
Dna Adducts ◽  
Blood Cells ◽  
White Blood Cells ◽  
Control Group ◽  
Study Group ◽  
Carcinogenic Potential ◽  
Potential Biomarker ◽  
Significant Difference ◽  
Polycyclic Aromatic ◽  
Peripheral White Blood Cells

Benzo[a]pyrene (B[a]P) is a major polycyclic aromatic hydrocarbon (PAH), it can bind the DNA to produce DNA-adducts, which has major carcinogenic potential. Enzyme-linked immunosorbet assay (ELISA) is the method used to detect these DNA adducts of B[a] P diolepoxide (BPDE) within the living cells.The aim of this study is to evaluate exposure to bitumen fumes, and to B[a]P in asphalt plant workers by measuring the BPDE-DNA adducts in their peripheral white blood cells (WBC), which are considered biological markers for exposure risk assessment.In this study, Hemostatic blood (CBC, AST) were measured, and the levels of BPDE-DNA adducts were measured in DNA samples of WBC obtained from asphalt plant workers in Syria and compared to those measured from a control group. The measurement was performed using BPDE-DNA Adducts ELISA.kit. The sample size was determined to be 50 with 25 asphalt plant workers and 25 healthy volunteers with no occupational exposure to PAHs.The results showed some diseases associated with exposure to asphalt fumes among the workers in the study group and a statistically significant difference in the values of (CBC; WBC, leukocytes, HCT, MCHC and AST) between the study group and the control group. BPDE-DNA adducts were detected in WBC of 11 asphalt plant workers with concentrations ranging between 0 and 2.75 ng/ml and only one individual in the control group with concentration of 0.75 ng/ml. These results indicate significant positive relationship between exposure to the bitumen fumes and formation of BPDE-DNA adducts.BPDE-DNA adducts is potential biomarker for PAHs exposure and likely helpful indicator of PAH-induced DNA damage and possibly carcinogenesis.

scholarly journals Effect of pregnancy on packed cell volume and total white blood cells count among Sudanese pregnant women attending antenatal care at Ribat University Hospital (Khartoum state)

2018 ◽  
Vol 7 (2) ◽  
pp. 371
Author(s):  
Wafa Mubarak M. Ahmed ◽  
Abdurrahman Khalid ◽  
O. A. Musa
Keyword(s):  
Antenatal Care ◽  
Pregnant Women ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
White Blood Cells ◽  
University Hospital ◽  
Control Group ◽  
P Values ◽  
Significant Difference

Background: During pregnancy the women undergo several structural and physiological changes almost in all body systems including the hematological profile. The main reported hematological change is the plasma expansion and haemodilution which affect the red blood cells(RBCs) and white blood cells (WBCs) count in addition to other hematological indices including packed cell volume (PCV). The objective of this study was to assess the effect of pregnancy on packed cell volume and white blood cells among Sudanese pregnant women and derive a formula for the normal values in pregnant women in relation to the packed cell volume.Methods: An analytical case control study was conducted in Khartoum state, Sudan 2017. The study included20 Sudanese pregnant women in the second and third trimesters attending the antenatal care at The National Ribat university hospital and 20 Sudanese non-pregnant women in the same age group as control. A questionnaire was used to collect socio-demographic and clinical data.5ml of venous blood was collected in a container containing EDTA. Complete blood count (CBC) was measured by using automated cell counter Sysmex.Results: There was statistically significant decrease in PCV value during 2nd and 3rd trimesters of pregnancy compared to the non-pregnant control group with p values (0.00 and 0.04 respectively), and statistically significant increase in WBCs count during 2nd and 3rd trimesters of pregnancy compared to the non-pregnant control group with p values (0.04 and 0.00 respectively). Also, a progressive increase in WBCs count with gestational age was demonstrated (second trimester 6.65×103/uL, third trimester 7.43×103/uL). The results showed mean WBCs of 7.79×103/uL, when compared to the estimated values the results showed no significant difference with the mean WBCs in 2ndand 3rdtrimesters (p value 0.06 and 0.39 respectively).Conclusions: During pregnancy there was statistically significant decrease in PCV values, statistically significant increase in WBCs count and by considering the haemodilution factor there was a higher increase in WBCs count.

scholarly journals Association of PAH-DNA adducts in peripheral white blood cells with dietary exposure to polyaromatic hydrocarbons.

1993 ◽  
Vol 99 ◽  
pp. 265-267 ◽  
Author(s):  
N Rothman ◽  
M C Poirier ◽  
R A Haas ◽  
A Correa-Villasenor ◽  
P Ford ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Blood Cells ◽  
Polyaromatic Hydrocarbons ◽  
White Blood Cells ◽  
Dietary Exposure ◽  
Peripheral White Blood Cells

Polycyclic aromatic hydrocarbon—DNA adducts in white blood cells from lung cancer patients: no correlation with adduct levels in lung

1992 ◽  
Vol 13 (6) ◽  
pp. 987-993 ◽  
Author(s):  
F.J. van Schooten ◽  
M.J.X. Hillebrand ◽  
F.E. van Leeuwen ◽  
N. van Zandwijk ◽  
H.M. Jansen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Polycyclic Aromatic Hydrocarbon ◽  
Aromatic Hydrocarbon ◽  
Cancer Patients ◽  
Dna Adducts ◽  
Blood Cells ◽  
White Blood Cells ◽  
Lung Cancer Patients ◽  
Polycyclic Aromatic

The effect of lead exposure on tracheal responsiveness to methacholine and ovalbumin, total and differential white blood cells count, and serum levels of immunoglobulin E, histamine, and cytokines in guinea pigs

2013 ◽  
Vol 33 (3) ◽  
pp. 325-333 ◽  
Author(s):  
T Farkhondeh ◽  
MH Boskabady ◽  
S Jalali ◽  
G Bayrami
Keyword(s):  
Blood Cells ◽  
Guinea Pigs ◽  
Lead Acetate ◽  
Immunoglobulin E ◽  
White Blood Cells ◽  
Serum Levels ◽  
Control Group ◽  
Significant Difference ◽  
Ifn Γ ◽  
Pb Concentration

The effect of exposure to inhaled lead acetate in guinea pigs was evaluated. The present study comprised of five groups of guinea pigs including control (C), sensitized to ovalbumin (OA; S) and three groups exposed to 0.1, 0.2, and 0.4 M inhaled lead (Pb; n = 6 for each group). Tracheal responsiveness to methacholine and OA, total and differential white blood cells (WBCs) count in lung lavage, serum levels of cytokines (interferon γ (IFN-γ) and interleukin 4 (IL-4)), histamines, and immunoglobulin E (IgE), and Pb concentration in lung were measured. Tracheal responsiveness to methacholine, OA, total and differential WBC types as well as IL-4, IFN-γ, histamine, and IgE were significantly increased but IFN-γ/IL-4 were significantly decreased in sensitized animals as well as those exposed to high Pb concentrations when compared with the control group (from p < 0.05 to p < 0.001). In addition, there was not a significant difference in most measured values between animals exposed to high Pb concentration and group S. The Pb concentration in lung tissues of animals exposed to all three Pb concentrations was significantly higher than that of group C ( p < 0.001 for all cases).These results showed that inhaled lead acetate exposure can induce lung inflammatory changes similar to sensitized animals. Therefore, exposure to environmental Pb pollution may cause asthma-like changes.

Monocytosis in rational empirical antibacterial therapy in moderate forms of COVID-19

2021 ◽  
Vol 2 (3(5)) ◽  
pp. 35-40
Author(s):  
A.V. Kovalev ◽  
◽  
M.I. Shperling ◽  
A.S. Polyakov ◽  
Ya.А. Nоskov ◽  
...  
Keyword(s):  
Antibacterial Therapy ◽  
Blood Cells ◽  
White Blood Cells ◽  
Absolute Number ◽  
Glucocorticoid Therapy ◽  
Control Group ◽  
Blood Monocytes ◽  
Significant Difference ◽  
Empirical Antibacterial Therapy ◽  
The Absolute

Microbiologically confirmed bacterial co-infection occurs in 1.2%–7% of hospitalized patients with COVID-19. The study of rational approaches to empirical antibacterial therapy (ABT) of SARS-CoV-2 virus-induced pneumonia continues. Glucocorticoid (GCS) therapy, the main method for pathogenetic treatment of moderate forms of CОVID-19, can lead to the development of neutrophilic leukocytosis. The criterion for the differential diagnosis of leukocytosis could be determining the quantity of peripheral blood monocytes. Assessing the significance of identifying the monocyte quantity can serve as an additional criterion for assigning empirical ABT in the treatment of pneumonia caused by the new coronavirus infection. The aim of the study was to identify the characteristics of glucocorticoid-induced leukocytosis in patients with moderate COVID-19. The study included 86 patients with a confirmed diagnosis of COVID-19 (ICD codes: U07.1, U07.2) of moderate severity. The patients were divided into 2 groups. The comparison group consisted of 40 patients who were prescribed ABT after the manifestation of leukocytosis on the background of glucocorticoid therapy. The control group included 46 people who were not prescribed ABT after the manifestation of leukocytosis on the background of glucocorticoid therapy and until the end of their stay in the hospital. We compared the parameters of the clinical blood tests (the absolute number of white blood cells, neutrophils and monocytes (×109/L)) on days 3, 6 and 9 from the start of GCS therapy. As a result, on the 3rd day, both groups had neutrophilic leukocytosis (>9.0×109/L) and absolute monocytosis (>0.8×109/L). There was a statistically signif icant decrease in the absolute number of white blood cells, neutrophils and monocytes by days 6 and 9, compared with day 3 from the start of glucocorticoid therapy. When comparing blood parameters between the groups, there was no statistically significant difference in the number of cells on the 3rd, 6th and 9th day of GCS therapy (p>0.05). Glucocorticoid-induced leukocytosis is associated with absolute monocytosis. The administration of ABT in response to the occurrence of leukocytosis in this study did not affect the change in the level of white blood cells. At the same time, a likely factor in reducing these indicators was a decrease in the daily dosage of corticosteroids.

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