scholarly journals Virulence Factors of Uropathogenic Escherichia coli

2021 ◽  
Author(s):  
Etefia Etefia
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cell Surface ◽  
Virulence Factors ◽  
Bacterial Cell ◽  
Uropathogenic Escherichia Coli ◽  
Bacterial Cell Surface

Uropathogenic Escherichia coli (UPEC) strains are those that cause infections in the urinary tract. They acquired virulence factors which enable them to survive in the urinary tract and elicit pathogenicity. The virulence factors are classified into two categories: (i) bacterial cell surface virulence factors and (ii) bacteria secreted virulence factors. Adhesins, toxins and iron up-take systems are major groups of virulence factors. The variety of virulence factors of UPEC is presented in this chapter.

scholarly journals Virulence factors of uropathogenic Escherichia coli of urinary tract infections and asymptomatic bacteriuria in children

2014 ◽  
Vol 47 (6) ◽  
pp. 455-461 ◽  
Author(s):  
Ki Wook Yun ◽  
Hak Young Kim ◽  
Hee Kuk Park ◽  
Wonyong Kim ◽  
In Seok Lim
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Virulence Factors ◽  
Urinary Tract Infections ◽  
Asymptomatic Bacteriuria ◽  
Uropathogenic Escherichia Coli ◽  
Tract Infections

scholarly journals An Approach to Uropathogenic Escherichia Coli in Urinary Tract Infections

2010 ◽  
Vol 2 (02) ◽  
pp. 070-073 ◽  
Author(s):  
K Prabhat Ranjan ◽  
Neelima Ranjan ◽  
Arindam Chakraborty ◽  
D R Arora
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cell Surface ◽  
Urinary Tract Infections ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Uropathogenic Escherichia Coli ◽  
Virulence Markers ◽  
The Difference ◽  
Tract Infections

ABSTRACT Purpose: To study the occurrence and characterization of Uropathogenic Escherichia coli (UPEC) in cases with urinary tract infections. Materials and Methods: A total of 220 symptomatic cases from urinary tract infections and 50 stool samples from apparently healthy individuals were included. The colonies identified as Escherichia coli were screened for virulence factors, that is, hemolysin, Mannose Resistant and Mannose Sensitive Hemagglutination (MRHA, MSHA), Cell surface hydrophobicity, and Serum resistance. Results: Among the 220 cases 91 (41.36%) were hemolytic, 68 (30.90%) showed MRHA, 58 (26.36%) were cell surface hydrophobicity positive, and 72 (32.72%) were serum-resistant. In 50 controls, three (6%) were hemolytic, six (12%) showed MRHA, nine (18%) showed cell surface hydrophobicity, and 12 (24%) were serum-resistant. The difference between cases and controls for hemolysis and MRHA were significant (P<0.001 and P<0.01, respectively). A total of 14 atypical E. coli were isolated from the urine and all showed the presence of one or the other virulence markers. Out of the 18 mucoid E.coli isolated, 10 were serum-resistant. Conclusions: The present study revealed that out of 220 urinary isolates, 151 could be labeled as UPEC.

Virulence Factors of the Bacterial Cell Surface

1978 ◽  
Vol 137 (5) ◽  
pp. 630-633 ◽  
Author(s):  
F. Orskov
Keyword(s):  
Cell Surface ◽  
Virulence Factors ◽  
Bacterial Cell ◽  
Bacterial Cell Surface

A surface-exposed DraD protein of uropathogenic Escherichia coli bearing Dr fimbriae may be expressed and secreted independently from DraC usher and DraE adhesin

Microbiology ◽  
2005 ◽  
Vol 151 (7) ◽  
pp. 2477-2486 ◽  
Author(s):  
Beata Zalewska ◽  
Rafał Piątek ◽  
Katarzyna Bury ◽  
Alfred Samet ◽  
Bogdan Nowicki ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Expression ◽  
Uropathogenic Escherichia Coli ◽  
Bacterial Attachment ◽  
Open Reading Frames ◽  
E Coli ◽  
Fimbrial Subunit ◽  
Reading Frames

The dra gene cluster, expressed by uropathogenic Escherichia coli strains, determines bacterial attachment and invasion. The Dr fimbrial structures formed at the bacterial cell surface are composed of DraE subunits. The Dr fimbriae-coding cluster contains six open reading frames – draA, draB, draC, draD, draP and draE – among which the draE gene encodes the structural fimbrial subunit DraE. Very little is known about E. coli surface expression of the draD gene product. The expression of DraD and its role in the biogenesis of Dr fimbriae were determined by constructing mutants in the dra operon and by immunoblot and immunofluorescence experiments. In this study, DraD was found to be a surface-exposed protein. The expression of DraD was independent of the DraC usher and DraE fimbrial subunits. Polymerization of DraE fimbrial subunits into fimbrial structures did not require expression of the DraD protein.

Functional Display of an Amoebic Chitinase in Escherichia coli Expressing the Catalytic Domain of EhCHT1 on the Bacterial Cell Surface

2020 ◽  
Vol 192 (4) ◽  
pp. 1255-1269
Author(s):  
Ricardo Torres-Bañaga ◽  
Rosa E. Mares-Alejandre ◽  
Celina Terán-Ramírez ◽  
Ana L. Estrada-González ◽  
Patricia L.A. Muñoz-Muñoz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Catalytic Domain ◽  
Bacterial Cell Surface

scholarly journals Heterogeneous Surface Expression of EspA Translocon Filaments by Escherichia coli O157:H7 Is Controlled at the Posttranscriptional Level

2003 ◽  
Vol 71 (10) ◽  
pp. 5900-5909 ◽  
Author(s):  
Andrew J. Roe ◽  
Helen Yull ◽  
Stuart W. Naylor ◽  
Martin J. Woodward ◽  
David G. E. Smith ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Population ◽  
Enteric Bacteria ◽  
Effector Proteins ◽  
Host Cells ◽  
Phase Variable ◽  
E Coli ◽  
Bacterial Cell Surface

ABSTRACT Type III secretion systems of enteric bacteria enable translocation of effector proteins into host cells. Secreted proteins of verotoxigenic Escherichia coli O157 strains include components of a translocation apparatus, EspA, -B, and -D, as well as “effectors” such as the translocated intimin receptor (Tir) and the mitochondrion-associated protein (Map). This research has investigated the regulation of LEE4 translocon proteins, in particular EspA. EspA filaments could not be detected on the bacterial cell surface when E. coli O157:H7 was cultured in M9 minimal medium but were expressed from only a proportion of the bacterial population when cultured in minimal essential medium modified with 25 mM HEPES. The highest proportions of EspA-filamented bacteria were detected in late exponential phase, after which filaments were lost rapidly from the bacterial cell surface. Our previous research had shown that human and bovine E. coli O157:H7 strains exhibit marked differences in EspD secretion levels. Here it is demonstrated that the proportion of the bacterial population expressing EspA filaments was associated with the level of EspD secretion. The ability of individual bacteria to express EspA filaments was not controlled at the level of LEE1-4 operon transcription, as demonstrated by using both β-galactosidase and green fluorescent protein (GFP) promoter fusions. All bacteria, whether expressing EspA filaments or not, showed equivalent levels of GFP expression when LEE1-4 translational fusions were used. Despite this, the LEE4-espADB mRNA was more abundant from populations with a high proportion of nonsecreting bacteria (low secretors) than from populations with a high proportion of secreting and therefore filamented bacteria (high secretors). This research demonstrates that while specific environmental conditions are required to induce LEE1-4 expression, a further checkpoint exists before EspA filaments are produced on the bacterial surface and secretion of effector proteins occurs. This checkpoint in E. coli O157:H7 translocon expression is controlled by a posttranscriptional mechanism acting on LEE4-espADB mRNA. The heterogeneity in EspA filamentation could arise from phase-variable expression of regulators that control this posttranscriptional mechanism.

scholarly journals Enhanced phagocytosis of encapsulated Escherichia coli strains after exposure to sub-MICs of antibiotics is correlated to changes of the bacterial cell surface.

1990 ◽  
Vol 34 (2) ◽  
pp. 332-336 ◽  
Author(s):  
G Raponi ◽  
N Keller ◽  
B P Overbeek ◽  
M Rozenberg-Arska ◽  
K P van Kessel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Bacterial Cell Surface
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