Morphological Changes Induced by Anticancer Drug Therapy of Ovarian Cancer Cells in Ascitic Fluid

Estrogens (Es) play a significant role in the carcinogenesis and progression of ovarian malignancies. Depending on the concentration, Es may have a protective or toxic effect on cells. Moreover, they can directly or indirectly affect the activity of membrane ion channels. In the presented study, we investigated in vitro the effectiveness of the ovarian cancer cells (MDAH-2774) pre-incubation with 17β-estradiol (E2; 10 µM) in the conventional chemotherapy (CT) and electrochemotherapy (ECT) with cisplatin or calcium chloride. We used three different protocols of electroporation including microseconds (µsEP) and nanoseconds (nsEP) range. The cytotoxic effect of the applied treatment was examined by the MTT assay. We used fluorescent staining and holotomographic imaging to observe morphological changes. The immunocytochemical staining evaluated the expression of the caspase-12. The electroporation process’s effectiveness was analyzed by a flow cytometer using the Yo-Pro™-1 dye absorption assay. We found that pre-incubation of ovarian cancer cells with 17β-estradiol may effectively enhance the chemo- and electrochemotherapy with cisplatin and calcium chloride. At the same time, estradiol reduced the effectiveness of electroporation, which may indicate that the mechanism of increasing the effectiveness of ECT by E2 is not related to the change of cell membrane permeability.

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Detection of morphological changes in cisplatin-treated ovarian cancer cells by digital holographic microscopy

2018 International Conference Laser Optics (ICLO) ◽

10.1109/lo.2018.8435576 ◽

2018 ◽

Author(s):

A.A. Zhikhoreva ◽

A.V. Belashov ◽

V.G. Bespalov ◽

V.A. Romanov ◽

A.L. Semenov ◽

...

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Morphological Changes ◽

Digital Holographic Microscopy ◽

Ovarian Cancer Cells ◽

Holographic Microscopy

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The anti-ovarian carcinoma activity of L-amino Acid Oxidase from Crotalus adamanteus venom in vivo and in vitro

10.21203/rs.3.rs-1131706/v1 ◽

2021 ◽

Author(s):

Li Bo ◽

Yan Xiong ◽

Qiyi He ◽

Xiaodong Yu ◽

Bo Li ◽

...

Keyword(s):

Ovarian Cancer ◽

Amino Acid ◽

Cancer Cells ◽

Morphological Changes ◽

Complex Mixture ◽

Ovarian Cancer Cells ◽

Acid Oxidase ◽

Amino Acid Oxidase ◽

Apoptosis Pathway

Abstract The anti-tumor potential of animal toxins has fully attracted the attention of researchers. Snake venoms is a complex mixture of different components and has revealed high toxicity on normal and tumoral tissues or cells. The snake venom L-Amino-acid oxidase (svLAAO) has grown up to be a critical research target in molecular biology sciences and medicine sciences since widespread presence and various biological roles, including antitumor application. We found that Crotalus adamanteus (C. adamanteus) venom LAAO significantly decreased the viability of ovarian cancer cells and caused morphological changes preceded cell death. Cell experiments confirmed that C. adamanteus venom LAAO caused alterations of intrinsic or extrinsic apoptosis pathway-related genes in ovarian cancer cells. Animal experiments and histological analysis also proved that C. adamanteus venom LAAO could effectively inhibit the damage of ovarian cancer to tissues. The major apoptosis induction of C. adamanteus venom LAAO on ovarian cancer cells can be blocked by catalase, suggesting that the cytotoxicity of C. adamanteus venom LAAO on ovarian cancer cells was mainly mediated by H2O2. Our preliminary results revealed that C. adamanteus venom LAAO may induce apoptosis of ovarian cancer cells through the death receptor pathway and mitochondrial pathway. It is inferred that C. adamanteus venom LAAO will be some advantages in New Drug Research and Development of antitumor drugs in the future. Nevertheless, extra studies on the pharmacological actions and molecular mechanism of svLAAO in anti-cancer are necessary in order to better promote its application.

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Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid

BIO-PROTOCOL ◽

10.21769/bioprotoc.3124 ◽

2018 ◽

Vol 8 (24) ◽

Author(s):

Vikash Kumar ◽

Asok Mukhopadhyay

Keyword(s):

Ovarian Cancer ◽

Natural Killer Cell ◽

Cancer Cells ◽

Natural Killer ◽

Ascitic Fluid ◽

Cell Function ◽

Killer Cell ◽

Ovarian Cancer Cells ◽

Human Ovarian Cancer ◽

Natural Killer Cell Function

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Chemical Analysis of Morphological Changes in Lysophosphatidic Acid-Treated Ovarian Cancer Cells

Scientific Reports ◽

10.1038/s41598-017-15547-7 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 3

Author(s):

Karen A. Bailey ◽

Yuliya Klymenko ◽

Peter E. Feist ◽

Amanda B. Hummon ◽

M. Sharon Stack ◽

...

Keyword(s):

Ovarian Cancer ◽

Chemical Analysis ◽

Cancer Cells ◽

Lysophosphatidic Acid ◽

Morphological Changes ◽

Ovarian Cancer Cells

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Sensitivity of EGFR/HER-2 Positive Cells Isolated from Ascitic Fluid of Advanced Ovarian Cancer Patients to EGFR/HER-2 Inhibitors

Applied Sciences ◽

10.3390/app10072343 ◽

2020 ◽

Vol 10 (7) ◽

pp. 2343

Author(s):

Kenny Chitcholtan ◽

Dianne Harker ◽

Bryony Simcock ◽

Peter Sykes

Keyword(s):

Ovarian Cancer ◽

Cancer Cells ◽

Cancer Patients ◽

Ascitic Fluid ◽

Cancer Cell ◽

Protein Level ◽

Advanced Ovarian Cancer ◽

Immunofluorescent Staining ◽

Ovarian Cancer Cells ◽

Her 2

Background: advanced ovarian cancer often presents with ascites. These ascites contain small clusters of cancer cells, which may contribute greatly to the metastatic potential of ovarian cancer in the peritoneal cavity. Therefore, understanding the unique protein expressions of this cell population will provide vital information for the development of tailored, targeted treatment. In this study, we isolate floating ovarian cancer cells from ovarian cancer patient ascitic fluid and use these cells to document that the expression of EGFR/HER-2 proteins may be essential for the growth and survival of these floating cancer cell clusters. Methods: ascitic fluid-derived cells were isolated from ascitic fluid by using Ficoll separation. Cells were cultured in a non-adherent condition for six days. The protein level of EGFR, HER-2, AKT, and ERK and their phosphorylation in ovarian cancer cell lines were determined by immunofluorescence. The immunofluorescent staining for proteins presented in ascitic fluid-derived cells determined the intensity profile of each protein using Carl Zeiss Blue software. Results: Isolated ovarian cancer cells from ascitic fluid have a measurable level of EGFR and HER-2 proteins. The inhibition of EGFR and EGFR/HER-2 positive cells with gefitinib and canertinib selectively disrupts cell viability and the protein level of EGFR, HER-2, AKT and ERK and their respective phosphorylation status. In addition, the dual EGFR/HER-2 inhibitor canertinib demonstrates greater anti-tumour effects than gefitinib in EGFR/HER-2 positive cells. Conclusion: These studies reveal an important role of multiple activation of receptor tyrosine kinases in floating ovarian cancer cells, as well as the importance of a dual EGFR/HER-2 inhibitor used as alternative adjuvant therapy in advanced ovarian cancer patients.

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