scholarly journals The Incidence of Current Infection with Different Human Malaria Species by Polymerase Chain Reaction for Diagnosis of Suspicious Malaria Patients on Elimination Region Sistan and Baluchistan Province, Southeast of Iran

2017 ◽  
Vol 10 (10) ◽  
Author(s):  
Adel Ebrahimzadeh ◽  
Sedigheh Nouri Dalir ◽  
Hadi Mirahmadi ◽  
Ahmad Mehravaran ◽  
Alireza Salimi Khorashad ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Human Malaria ◽  
Current Infection ◽  
Malaria Species ◽  
Polymerase Chain

scholarly journals Usefulness of Polymerase Chain Reaction in the diagnosis of asymptomatic malaria among school age children in Ilorin, Nigeria

2018 ◽  
Vol 1 (1) ◽  
pp. 39-45
Author(s):  
Ayodele Adedoja ◽  
Shola Kola Babatunde ◽  
Bukola Deborah Tijani ◽  
Ajibola A. Akanbi II ◽  
Olusola Ojurongbe
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Species ◽  
Malaria Diagnosis ◽  
School Age Children ◽  
Clinical Settings ◽  
Asymptomatic Malaria ◽  
Chain Reaction ◽  
Malaria Species ◽  
Pcr Method ◽  
Polymerase Chain

Introduction: In Nigeria, microscopy and Rapid Diagnostic Tests (RDTs) are majorly used routinely to diagnose malaria in clinical settings. In recent years, molecular diagnosis has emerged as the most sensitive method for malaria diagnosis. This study determined the usefulness of Polymerase Chain Reaction (PCR) in comparison to microscopy (Giemsa stained thick and thin smears) for the diagnosis of asymptomatic malaria in Ilorin, Nigeria. Methods: Th e study enrolled 310 school children aged 4–15 years with no sign or symptoms of malaria. Blood samples were collected for identification of Plasmodium species infection using light microscopy and conventional PCR. Results: The PCR method detected m o r e infection of P. falciparum 107 (34.5%) than slide microscopy 81 (26.1%) in the study. Only P. falciparum was detected by microscopy while PCR detected mono infection of P. malariae (3.2%) and P. ovale (0.6%) and mixed infection of P. falciparum and P. malariae (3.2%). Overall the PCR method detected more malaria parasite compared to microscopy. Conclusion: The PCR technique, although more laborious and expensive than microscopy, have better diagnostic accuracy and are highly useful for the detection of P. falciparum and other malaria species in asymptomatic and low parasitaemia cases.

Standardization of a Polymerase Chain Reaction Protocol for Detecting Antimicrobial Peptides in Anopheles Darlingi (Diptera: Culicidae) Mosquitoes from the Brazilian Amazon

2021 ◽  
Author(s):  
Erian Santos ◽  
Ana Cecília Feio dos Santos ◽  
Luciana Letícia da Costa Pires ◽  
Samir Mansour Moraes Casseb ◽  
Gustavo Moraes Holanda ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Antimicrobial Peptides ◽  
Brazilian Amazon ◽  
The State ◽  
Anopheles Darlingi ◽  
Chain Reaction ◽  
Human Malaria ◽  
Annealing Temperatures ◽  
Polymerase Chain ◽  
Anopheline Mosquitoes

Abstract Background: Antimicrobial peptides (AMPs) are proteins of the innate immune system that can limit infections of the malaria-carrying parasite Plasmodium, which develops inside anopheline mosquitoes, the human malaria vectors. Despite this, studies on Brazilian Amazon species of anopheline mosquitoes are still needed. The aim of the study is to develop a standard molecular polymerase chain reaction (PCR) technique to detect the AMPs cecropin A (CecA) and defensin from Anopheles darlingi to support studies involving their detection and amplification and better understanding of the roles of these peptides in the Anopheles-Plasmodium interaction. Methods: The collection of anopheline mosquitoes was carried out in three municipalities in the Brazilian Amazon: Altamira and Peixe-Boi, in the state of Pará, and Cruzeiro do Sul, in the state of Acre. The primers were built based on the sequences available in GenBank, and PCR followed standard protocols with different annealing temperatures tested. The PCR products were purified and then sequenced by the dideoxy chain termination method. Results: CecA and defensin amplification were standardized with annealing temperatures of 59ºC and 55ºC, respectively. The amplified products and sequencing demonstrated the good quality of both primer sets. Conclusions: For the first time, a standardized molecular technique for detecting AMPs was described in An. darlingi, a mosquito species from the Brazilian Amazon, supporting future studies aiming to understand the interactions of this species and the action of these peptides during infection and providing important molecular markers for the control of human malaria.

High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction

1993 ◽  
Vol 61 (2) ◽  
pp. 315-320 ◽  
Author(s):  
Georges Snounou ◽  
Suganya Viriyakosol ◽  
Xin Ping Zhu ◽  
William Jarra ◽  
Lucilia Pinheiro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Sensitivity ◽  
Chain Reaction ◽  
Malaria Parasites ◽  
Nested Polymerase Chain Reaction ◽  
Human Malaria ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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