Epithelioid Leiomyosarcoma With Osteoclast-like Giant Cells in the Rectum

2000 ◽  
Vol 124 (3) ◽  
pp. 438-440 ◽  
Author(s):  
Tadashi Terada ◽  
Kanenori Endo ◽  
Hiroyuki Maeta ◽  
Satoshi Horie ◽  
Tetsuo Ohta
Keyword(s):  
Tumor Cells ◽  
Biopsy Specimen ◽  
Smooth Muscle Actin ◽  
Cell Types ◽  
Giant Cells ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
First Case ◽  
Old Japanese ◽  
Giant Tumor

Abstract We report a rare case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells. A 71-year-old Japanese man was admitted to a hospital with melena. Results of a colonoscopy test revealed a polypoid tumor in the rectum, and a biopsy specimen from the lesion showed a sarcoma; the patient underwent rectosigmoidectomy. At gross inspection, the tumor measured 8 × 7 × 4 cm and was polypoid with ulcerations. Necrotic and hemorrhagic foci were scattered. Microscopically, the tumor consisted of 2 cell types: malignant tumor cells with epithelioid features and benign-appearing osteoclast-like giant cells. The tumor cells were polygonal and epithelioid in shape and had eosinophilic or clear cytoplasms, with scattered giant tumor cells. Immunohistochemical examination revealed that the tumor cells were positive for vimentin, muscle actin, α-smooth muscle actin, and desmin, whereas the osteoclast-like giant cells were positive for CD68, leukocyte common antigen, and lysozymes. We diagnosed this case as epithelioid leiomyosarcoma with osteoclast-like giant cells. To the best of our knowledge, this is the first case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells.

scholarly journals Vaginal fibroleiomyoma in a cow: a case report

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 138-141 ◽  
Author(s):  
N. Timurkaan ◽  
M. Aydin ◽  
F. Yilmaz ◽  
A. Cevik
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Giant Cells ◽  
Vaginal Wall ◽  
Muscle Actin ◽  
Vaginal Route ◽  
Immunohistochemical Examination ◽  
Brown Swiss ◽  
Cd 68 ◽  
The Masses

: This paper describes a case of fibroleiomyoma seen in the vagina of a cow, diagnosed on the basis of macroscopic, microscopic and immunohistochemical findings. A five year-old female, Simmental and Brown Swiss crossbreed cow presented with six neoplastic masses located on the vaginal wall. The masses were surgically removed through the vaginal route and were firm and well demarcated. Microscopic examination showed that the non-encapsulated neoplastic nodules consisted of the admixture of smooth muscle and connective tissue. Immunohistochemical examination revealed strong focal positive reactions for smooth muscle actin and vimentin, but no positive reaction for CD 68. The tumour reported here was considered benign because of the lack of clear pleomorphism, invasivness, multinuclear giant cells and atypia, and low mitotic activity.

scholarly journals Preoperatively diagnosed gastric collision tumor with mixed adenocarcinoma and gastrointestinal stromal tumor: a case report and literature review

2021 ◽  
Author(s):  
Kunihiko Matsuno ◽  
Yoshikazu Kanazawa ◽  
Daisuke Kakinuma ◽  
Nobutoshi Hagiwara ◽  
Fumihiko Ando ◽  
...  
Keyword(s):  
Gastrointestinal Stromal Tumor ◽  
Distal Gastrectomy ◽  
Submucosal Tumor ◽  
Smooth Muscle Actin ◽  
Collision Tumor ◽  
Stromal Tumor ◽  
Lower Body ◽  
Muscle Actin ◽  
First Case ◽  
S 100

AbstractReports of gastric collision tumors, comprising adenocarcinoma and gastrointestinal stromal tumor, are extremely rare. Here, we report the case of a 68-year-old male who was diagnosed with a lower-body, moderately differentiated, tubular-type adenocarcinoma and submucosal tumor and underwent an elective D2 distal gastrectomy. The tumor cells of the gastrointestinal stromal tumor were positive for H-caldesmon and CD117, weakly positive for smooth muscle actin and DOG-1, and negative for desmin, S-100 protein, CD31, and AE1/AE3. The tumor had grown into a mixed form of adenocarcinoma and gastrointestinal stromal tumor. Thus, we report the first case of a preoperatively diagnosed collision tumor in the stomach consisting of adenocarcinoma and gastrointestinal stromal tumor.

Primary Glomangioma of the Liver: A Case Report and Review of the Literature

2004 ◽  
Vol 128 (3) ◽  
pp. e46-e49 ◽  
Author(s):  
Vilkesh R. Jaiswal ◽  
Julie G. Champine ◽  
Suash Sharma ◽  
Kyle H. Molberg
Keyword(s):  
Tumor Cells ◽  
Smooth Muscle Actin ◽  
Vascular Network ◽  
Pericellular Matrix ◽  
Muscle Actin ◽  
Review Of The Literature ◽  
Deep Soft Tissue ◽  
Large Tumor ◽  
Glomus Tumors

Abstract Glomangiomas are a subset of glomus tumors that have a rich vascular network. Although a majority of the glomus tumors occur in the skin of the hand, they have also been reported in the deep soft tissue, bone, lungs, and gastrointestinal tract, especially the stomach. To our knowledge, only one such case has previously been reported primarily occurring in the liver. We report a case of a glomangioma primarily arising in the liver of a 57-year-old man who presented with right flank pain of several months' duration. A 3.0-cm hepatic mass was excised and consisted of numerous, small-to-medium branched vessels with the stroma containing small, round, regular cells with sharply outlined round-to-oval nuclei. Immunostains showed the tumor cells to be diffusely positive for vimentin and smooth muscle actin and to be focally positive for calponin. Collagen IV stained the pericellular matrix. The immunostain for CD34 highlighted the vascular network as well as outlined the tumor cells in many areas. Coexpression of actin and CD34 in glomus tumors, although unusual, has recently been reported in the literature. Despite its bland histology, the large tumor size and deep visceral location were suggestive of aggressive behavior; thus, a close clinical follow-up was recommended. The patient had an unremarkable postoperative course and has no evidence of metastatic disease 12 months after the procedure. An accurate diagnosis and an understanding the biology of this rare disease, especially in an unusual location, are crucial to its management.

scholarly journals Is Pneumatosis Cystoides Intestinalis Gas-Distended and Ruptured Lymphatics?: Reappraisal by Immunohistochemistry

2014 ◽  
Vol 138 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Xianyong Gui ◽  
Yi Zhou ◽  
Leslie Eidus ◽  
Vincent Falck ◽  
Zu-hua Gao ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Linear Structure ◽  
Giant Cells ◽  
Pneumatosis Cystoides Intestinalis ◽  
Muscle Actin ◽  
Lymphatic Endothelial Cells ◽  
Diverse Background ◽  
The Right ◽  
Age Range

Context.—Pneumatosis cystoides intestinalis (PCI) is a condition with multiple gas-filled cysts within the bowel wall, associated with diverse background diseases. Its pathogenesis is still a mystery. Some previous observations scattered in the literature have suggested an association of the cystic spaces in PCI with the lymphatics. Objective.—To further investigate whether PCI results from the ballooning of gas-filled lymphatic channels. Design.—We did immunostaining of podoplanin, a mucoprotein preferentially expressed in lymphatic endothelial cells, in 13 cases (8 men, 5 women; age range, 18–80 years) of PCI. Ten cases were diagnosed in resected segments of bowel and 3 in biopsies. Pneumatosis was seen in the right side of the colon (9 cases), transverse colon (1 case), sigmoid colon (1 case), and small bowel (2 cases). In addition, immunostaining for CD31, calretinin, WT1, CD68, smooth muscle actin, desmin, vimentin, and cytokeratins was also performed for comparison and correlation. Results.—A strong immunopositivity of podoplanin was seen in a condensed linear structure in the pericystic interstitium in 100% of the cases, but was not seen in the overlying giant and flat cells that were all CD68-positive histiocytes. Meanwhile, the podoplanin-expressing structure was negative for calretinin and WT1, which ruled out the possible mesothelial origin. There were coexistent variable immunopositivity of smooth muscle actin, which suggests an admixture of myofibroblasts. These findings indicated that the PCI cases were gas-distended lymphatics with the lymphatic epithelium ruptured and embedded in the reactive histiocytes and giant cells. Conclusion.—Our findings support the lymphatic theory about the pathogenesis of PCI.

scholarly journals Uterine Leiomyosarcoma Associated with Cystic Endometrial Polyps, Chondriod Metaplasia and Uterine Horn Intussusception in a Greater Cane Rat (Thryonomys swinderianus)

2020 ◽  
Vol 43 (2) ◽  
pp. 197-204
Author(s):  
Olusola Lawrence Ajayi ◽  
Moshood Olajire Olaniyi ◽  
Olugbenga Olayinka Alaka ◽  
Richard Edem Antia ◽  
Temitope Morenikeji Oladipo
Keyword(s):  
Smooth Muscle ◽  
Rare Case ◽  
Smooth Muscle Actin ◽  
Uterine Horn ◽  
Uterine Leiomyosarcoma ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
Endometrial Polyps ◽  
Greater Cane Rat ◽  
Positive Immunoreactivity

AbstractA rare case of uterine leiomyosarcoma associated with chondriod metaplasia, cystic endometrial polyps and uterine horn intussusception in a greater cane rat was macroscopically, histopathologically, immuno-histochemically and ultrastructurally evaluated. The histopathological findings for this tumour were similar to those for leiomyosarcomas described in other species. Immunohistochemical examination demonstrated positive immunoreactivity of neoplastic cells with α-smooth muscle actin, desmin and vimentin. Ultrastructurally, nuclear and cytoplasmic features were consistent with leiomyosarcoma. These results revealed the tumour to be of smooth muscle origin. To our knowledge, this is the first reported case of uterine leiomyosarcoma associated with cystic endometrial polyps, chondriod metaplasia and uterine horn intussusception in a greater cane rat.

Intraocular Inflammatory Myofibroblastic Tumor With ALK Overexpression

2004 ◽  
Vol 128 (1) ◽  
pp. e5-e7
Author(s):  
Dennis P. O'Malley ◽  
Christopher Poulos ◽  
Magdalena Czader ◽  
Warren G. Sanger ◽  
Attilio Orazi
Keyword(s):  
Tumor Cells ◽  
Inflammatory Myofibroblastic Tumor ◽  
Plasma Cells ◽  
Smooth Muscle Actin ◽  
Chromosome 2 ◽  
Muscle Actin ◽  
Anaplastic Lymphoma ◽  
Spindle Cells ◽  
Multiple Copies

Abstract We report a case of an intraocular inflammatory myofibroblastic tumor nearly filling the vitreous cavity of the eye of a 50-year-old man. The tumor was composed of a mixture of spindle cells and mixed inflammatory elements, including numerous plasma cells. The differential diagnosis included inflammatory pseudotumor and neoplastic mimics of this condition. Further investigation with immunohistochemistry revealed the mass to be composed of myofibroblasts, positive for smooth muscle actin stains and with weak anaplastic lymphoma kinase (ALK) expression in some tumor cells. Evaluation by fluorescence in situ hybridization revealed the tumor cells to have multiple copies of chromosome 2 and ALK but no rearrangement of the ALK gene. The authors propose that multiple copies of the ALK gene may be involved in inflammatory myofibroblastic tumor tumorigenesis, in addition to ALK gene rearrangements.

scholarly journals Host-Derived Pericytes and Sca-1+ Cells Predominate in the MART-1− Stroma Fraction of Experimentally Induced Melanoma

2011 ◽  
Vol 59 (12) ◽  
pp. 1060-1075 ◽  
Author(s):  
J. Humberto Treviño-Villarreal ◽  
Douglas A. Cotanche ◽  
Rosalinda Sepúlveda ◽  
Magda E. Bortoni ◽  
Otto Manneberg ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Smooth Muscle Actin ◽  
Cell Types ◽  
Muscle Actin ◽  
Cellular Composition ◽  
Tumor Capsule ◽  
Form Blood Vessel

Identification of cell types in tumor-associated stroma that are involved in the development of melanoma is hampered by their heterogeneity. The authors used flow cytometry and immunohistochemistry to demonstrate that anti–MART-1 antibodies can discriminate between melanoma and stroma cells. They investigated the cellular composition of the MART-1−, non-hematopoietic melanoma-associated stroma, finding it consisted mainly of Sca-1+ and CD146+ cells. These cell types were also observed in the skin and muscle adjacent to developing melanomas. The Sca-1+ cell population was observed distributed in the epidermis, hair follicle bulges, and tumor capsule. The CD146+ population was found distributed within the tumor, mainly associated with blood vessels in a perivascular location. In addition to a perivascular distribution, CD146+ cells expressed α-smooth muscle actin, lacked expression of endothelial markers CD31 and CD34, and were therefore identified as pericytes. Pericytes were found to be associated with CD31+ endothelial cells; however, some pericytes were also observed associated with CD31−, MART-1+ B16 melanoma cells that appeared to form blood vessel structures. Furthermore, the authors observed extensive nuclear expression of HIF-1α in melanoma and stroma cells, suggesting hypoxia is an important factor associated with the melanoma microenvironment and vascularization. The results suggest that pericytes and Sca-1+ stroma cells are important contributors to melanoma development.

scholarly journals Multiple Histological Subtypes of Dermatofibrosarcoma Protuberans Occurring in the Same Tumor

2015 ◽  
Vol 53 (1) ◽  
pp. 81-90
Author(s):  
C. Socoliuc ◽  
Sabina Zurac ◽  
R. Andrei ◽  
Florica Stăniceanu
Keyword(s):  
Tumor Cells ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Dermatofibrosarcoma Protuberans ◽  
Giant Cells ◽  
Negative Staining ◽  
Low Grade ◽  
Body Type ◽  
Morphological Aspects ◽  
Resection Specimen

AbstractDermatofibrosarcoma protuberans (DFSP) represents a low-grade cutaneous sarcoma which may have different histological aspects, presenting as a fibrosarcomatous, pigmented, juvenile, myxoid, atrophic, sclerosing or myoid lesion. Some of these subtypes may occur isolated or in association with one of the others creating hybrid lesions. We present the case of a 66 years old woman having a 4 cm diameter tumor located on the abdominal wall. Histopathological examination of the resection specimen revealed areas of typical DFSP associated with fibrosarcomatous transformation, myoid and myxoid areas. Also, focally, pleomorphic tumor cells and foreign-body type multinucleated giant cells were observed. Immunostains revealed CD34 positivity in typical DFSP and myxoid areas with negative staining of some of the tumor cells in fibrosarcomatous areas and negative staining of myoid areas. Smooth muscle actin was positive in myoid areas. The nature of myoid fascicles in DFSP is a matter of debate, being uncertain whether these represent a type of tumor differentiation or a reactive myoid proliferation. In this particular case, finding the association of myoid cells with blood vessel walls sustains their reactive nature. We present the morphological aspects of the different areas of the tumor with emphasis on differential diagnostic problems and clinical implications.

scholarly journals Primary Leiomyosarcoma of the Penis: A Review and Update of the Literature

2019 ◽  
pp. 43-51
Author(s):  
Anthony Kodzo-Grey Venyo
Keyword(s):  
Tumor Cells ◽  
Tunica Albuginea ◽  
Smooth Muscle Actin ◽  
Giant Cells ◽  
Resection Margins ◽  
Positive Staining ◽  
Alpha Smooth Muscle Actin ◽  
Complete Excision ◽  
Eosinophilic Cytoplasm ◽  
Abundant Eosinophilic Cytoplasm

Less than 50 cases of Primary Leiomyosarcomas of the Penis (PLOP) have been reported despite this PLOPs are the second most common sarcomas of the penis. The usual site for the development of PLOP is the shaft or base of the penis but the malignancy can affect any site of the penis. PLOPs are likely to be superficially located above the tunica albuginea in comparison with deep seated PLOPs. Superficial PLOPs may be asymptomatic or they may present as lumps, ulcers or nodules on the penis. Deep PLOPs may manifest with dysuria and difficulty with micturition when they compress the urethra. Diagnosis of PLOP is based upon the pathology examination features of specimens of the penile lesion that show interlacing fascicles of spindled-cells that have abundant eosinophilic cytoplasm, focal juxta-nuclear vacuoles and blunt ended nuclei and moderate to severe nuclear atypia, rarely atypical multi-nucleated giant cells and focal necrosis tend to be seen. There may be evidence of many mitoses. Immunohistochemistry studies of PLOP tend to show tumor cells that exhibit positive staining for desmin, muscle specific-actin, and alpha smooth muscle actin. Most often superficial PLOPs tend to be treated by wide complete excision of the tumor with tumor free and clear surgical resection margins. Deep seated PLOPs have also been treated by complete excision in various forms depending upon the site and size of the tumor and some of these have included Wide excision, partial penectomy and total penectomy alone and sometimes with adjuvant chemotherapy/radiotherapy. Some cases of deep PLOPs tend to be associated with the development of recurrences and metastases as well as death of the patient and hence deep PLOPs are aggressive tumors. Some people are of the opinion that chemotherapy and radiotherapy are not effective for the adjuvant treatment of PLOP. For this reason it would appear that there is need for the development of new chemotherapy medicaments that would effectively destroy PLOP tumor cells. There is need to undertake a global multi-center treatment trial of immunotherapy, chemotherapy and radiotherapy in order to streamline the treatment of PLOPs especially deep PLOPs.

scholarly journals Occurrence of an Abdominal Leiomyosarcoma in the Cyprinus carpio: Optical and Immunohistochemical Study

2021 ◽  
pp. 10-19
Author(s):  
Luis Alberto Romano ◽  
Virgínia Fonseca Pedrosa
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Immunohistochemical Study ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Swim Bladder ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
Vortex Pattern ◽  
Spindle Cells

A capsulated nodular neoplasm measuring 9 cm in diameter, located between the swim bladder and the kidney sample of Cyprinuscarpio, was in laboratory. The neoplasm had a solid consistency in one sector and very adherent to the capsule. Histopathological examination of the neoplasm revealed a monotonous proliferation of spindle cells, and pleomorphic, sometimes in a vortex pattern and interspersed with collagen fibers. Cells had numerous well-oriented myofibrils giving them a deep red. The cytoplasm is eosinophilic and the nuclei are hyperchromatic located in the center with blunt or "cigar-shaped" ends. In the immunohistochemical examination, the tumor cells were positive for desmin, smooth muscle actin and K-47, the latter antibody showing significant cell proliferation. Due to histopathological and immunohistochemical findings, diagnosis was made with leiomyosarcoma.

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