scholarly journals Vaginal fibroleiomyoma in a cow: a case report

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 138-141 ◽  
Author(s):  
N. Timurkaan ◽  
M. Aydin ◽  
F. Yilmaz ◽  
A. Cevik
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Giant Cells ◽  
Vaginal Wall ◽  
Muscle Actin ◽  
Vaginal Route ◽  
Immunohistochemical Examination ◽  
Brown Swiss ◽  
Cd 68 ◽  
The Masses

: This paper describes a case of fibroleiomyoma seen in the vagina of a cow, diagnosed on the basis of macroscopic, microscopic and immunohistochemical findings. A five year-old female, Simmental and Brown Swiss crossbreed cow presented with six neoplastic masses located on the vaginal wall. The masses were surgically removed through the vaginal route and were firm and well demarcated. Microscopic examination showed that the non-encapsulated neoplastic nodules consisted of the admixture of smooth muscle and connective tissue. Immunohistochemical examination revealed strong focal positive reactions for smooth muscle actin and vimentin, but no positive reaction for CD 68. The tumour reported here was considered benign because of the lack of clear pleomorphism, invasivness, multinuclear giant cells and atypia, and low mitotic activity.

scholarly journals Is Pneumatosis Cystoides Intestinalis Gas-Distended and Ruptured Lymphatics?: Reappraisal by Immunohistochemistry

2014 ◽  
Vol 138 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Xianyong Gui ◽  
Yi Zhou ◽  
Leslie Eidus ◽  
Vincent Falck ◽  
Zu-hua Gao ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Linear Structure ◽  
Giant Cells ◽  
Pneumatosis Cystoides Intestinalis ◽  
Muscle Actin ◽  
Lymphatic Endothelial Cells ◽  
Diverse Background ◽  
The Right ◽  
Age Range

Context.—Pneumatosis cystoides intestinalis (PCI) is a condition with multiple gas-filled cysts within the bowel wall, associated with diverse background diseases. Its pathogenesis is still a mystery. Some previous observations scattered in the literature have suggested an association of the cystic spaces in PCI with the lymphatics. Objective.—To further investigate whether PCI results from the ballooning of gas-filled lymphatic channels. Design.—We did immunostaining of podoplanin, a mucoprotein preferentially expressed in lymphatic endothelial cells, in 13 cases (8 men, 5 women; age range, 18–80 years) of PCI. Ten cases were diagnosed in resected segments of bowel and 3 in biopsies. Pneumatosis was seen in the right side of the colon (9 cases), transverse colon (1 case), sigmoid colon (1 case), and small bowel (2 cases). In addition, immunostaining for CD31, calretinin, WT1, CD68, smooth muscle actin, desmin, vimentin, and cytokeratins was also performed for comparison and correlation. Results.—A strong immunopositivity of podoplanin was seen in a condensed linear structure in the pericystic interstitium in 100% of the cases, but was not seen in the overlying giant and flat cells that were all CD68-positive histiocytes. Meanwhile, the podoplanin-expressing structure was negative for calretinin and WT1, which ruled out the possible mesothelial origin. There were coexistent variable immunopositivity of smooth muscle actin, which suggests an admixture of myofibroblasts. These findings indicated that the PCI cases were gas-distended lymphatics with the lymphatic epithelium ruptured and embedded in the reactive histiocytes and giant cells. Conclusion.—Our findings support the lymphatic theory about the pathogenesis of PCI.

scholarly journals Uterine Leiomyosarcoma Associated with Cystic Endometrial Polyps, Chondriod Metaplasia and Uterine Horn Intussusception in a Greater Cane Rat (Thryonomys swinderianus)

2020 ◽  
Vol 43 (2) ◽  
pp. 197-204
Author(s):  
Olusola Lawrence Ajayi ◽  
Moshood Olajire Olaniyi ◽  
Olugbenga Olayinka Alaka ◽  
Richard Edem Antia ◽  
Temitope Morenikeji Oladipo
Keyword(s):  
Smooth Muscle ◽  
Rare Case ◽  
Smooth Muscle Actin ◽  
Uterine Horn ◽  
Uterine Leiomyosarcoma ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
Endometrial Polyps ◽  
Greater Cane Rat ◽  
Positive Immunoreactivity

AbstractA rare case of uterine leiomyosarcoma associated with chondriod metaplasia, cystic endometrial polyps and uterine horn intussusception in a greater cane rat was macroscopically, histopathologically, immuno-histochemically and ultrastructurally evaluated. The histopathological findings for this tumour were similar to those for leiomyosarcomas described in other species. Immunohistochemical examination demonstrated positive immunoreactivity of neoplastic cells with α-smooth muscle actin, desmin and vimentin. Ultrastructurally, nuclear and cytoplasmic features were consistent with leiomyosarcoma. These results revealed the tumour to be of smooth muscle origin. To our knowledge, this is the first reported case of uterine leiomyosarcoma associated with cystic endometrial polyps, chondriod metaplasia and uterine horn intussusception in a greater cane rat.

scholarly journals Occurrence of an Abdominal Leiomyosarcoma in the Cyprinus carpio: Optical and Immunohistochemical Study

2021 ◽  
pp. 10-19
Author(s):  
Luis Alberto Romano ◽  
Virgínia Fonseca Pedrosa
Keyword(s):  
Cell Proliferation ◽  
Smooth Muscle ◽  
Immunohistochemical Study ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Swim Bladder ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
Vortex Pattern ◽  
Spindle Cells

A capsulated nodular neoplasm measuring 9 cm in diameter, located between the swim bladder and the kidney sample of Cyprinuscarpio, was in laboratory. The neoplasm had a solid consistency in one sector and very adherent to the capsule. Histopathological examination of the neoplasm revealed a monotonous proliferation of spindle cells, and pleomorphic, sometimes in a vortex pattern and interspersed with collagen fibers. Cells had numerous well-oriented myofibrils giving them a deep red. The cytoplasm is eosinophilic and the nuclei are hyperchromatic located in the center with blunt or "cigar-shaped" ends. In the immunohistochemical examination, the tumor cells were positive for desmin, smooth muscle actin and K-47, the latter antibody showing significant cell proliferation. Due to histopathological and immunohistochemical findings, diagnosis was made with leiomyosarcoma.

scholarly journals Congenital Cutaneous Kaposiform Hemangioendothelioma in a Newborn Calf

2020 ◽  
Vol 43 (2) ◽  
pp. 193-196
Author(s):  
Erkmen Tuğrul Epikmen ◽  
Ahmet Aydogan ◽  
Hamdi Avci ◽  
Sümbül Serap Birincioğlu
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Tumor Cells ◽  
Histopathological Examination ◽  
Smooth Muscle Actin ◽  
Whole Body ◽  
Muscle Actin ◽  
Alpha Smooth Muscle Actin ◽  
Kaposiform Hemangioendothelioma ◽  
The Masses

AbstractA one-day-old female Holstein calf was presented with subcutaneous masses spread over the whole body. Macroscopically, the masses were firm in touch, greyish-white in colour, 0.5-2 cm in diameter range. Histopathological examination confirmed the cutaneous Kaposiform hemangioendothelioma (KHE). Microscopic examination of the tumor revealed sheets of spindled endothelial cells forming vascular slits. Immunohistochemically, the tumor cells and capillaries gave strongly positive reaction for CD31 while vimentin, alpha smooth muscle actin and cytokeratin AE1/AE3 were negative. In this case, macroscopical, detailed histhopathological and immunohistochemical findings of congenital KHE reported firstly in a newborn calf.

Dacryops (Lacrimal Cyst) in Three Young Labrador Retrievers

2009 ◽  
Vol 45 (4) ◽  
pp. 191-196 ◽  
Author(s):  
Juri Ota ◽  
Jacqueline W. Pearce ◽  
Michael J. Finn ◽  
Gayle C. Johnson ◽  
Elizabeth A. Giuliano
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Actin ◽  
Case Series ◽  
Surgical Excision ◽  
Cystic Mass ◽  
Muscle Actin ◽  
Aspiration Cytology ◽  
History Of ◽  
The Masses ◽  
Labrador Retrievers

This case series constitutes a report of dacryops in multiple Labrador retrievers and the use of smooth-muscle actin immunostaining to confirm the lacrimal duct origins of the cyst wall. Three Labrador retrievers were presented with a history of a slowly enlarging mass adjacent to the left medial canthus. Ultrasonography of the masses revealed they were each spherical, thin-walled cystic structures. Aspiration cytology was performed in two cases revealing mixed inflammation and absence of detectable microorganisms. Dacryocystorhinography of the left nasolacrimal system performed in two cases revealed a normal nasolacrimal system that was closely associated, but not communicating with, the cystic mass in both cases. Surgical excision of all cysts was curative. Histopathology and positive immunohistochemical staining for smooth-muscle actin confirmed a diagnosis of dacryops in all cases.

scholarly journals Piloleiomyosarcoma in cats: Histological and immunohistochemical features

2021 ◽  
pp. 030098582110425
Author(s):  
Francisco Rodríguez Guisado ◽  
Pedro Luis Castro
Keyword(s):  
Smooth Muscle ◽  
Subcutaneous Tissue ◽  
Smooth Muscle Actin ◽  
Neuron Specific Enolase ◽  
Surgical Excision ◽  
Giant Cells ◽  
Hair Follicles ◽  
Muscle Actin ◽  
Neoplastic Cells ◽  
S 100

This study describes the histomorphology and immunohistochemical profile of 9 cases of feline piloleiomyosarcoma. Cats ranged in age from 7 to 16 years (mean 10), and tumors were 7 to 24 mm in diameter (mean 15). Tumors were composed of fusiform cells that were haphazardly arranged or in variably sized interwoven bundles. Neoplastic cells had eosinophilic and fibrillar cytoplasm, and elongated blunt-ended nuclei. Entrapment of hair follicles and absence of vascular components support an origin from the smooth muscle cells of the arrector pili. Additional findings included bizarre nuclei and giant cells (7/9 cases), atypical mitoses (7/9 cases), ulceration (3/9 cases), and intratumoral necrosis (6/9 cases). Neoplastic cells expressed calponin, desmin, α-smooth muscle actin, and vimentin, but not CD18, CD31, cytokeratins, glial fibrillary acidic protein, neuron-specific enolase, Melan A, p63, or S-100 protein. Surgical excision was curative in 6/9 cases, with local recurrence in 2/9 cases and metastasis to local lymph nodes in 1/9 case. Clinical outcome was influenced by mitotic count, infiltration of subcutaneous tissue, and intensity of nuclear immunolabeling for p53.

Epithelioid Leiomyosarcoma With Osteoclast-like Giant Cells in the Rectum

2000 ◽  
Vol 124 (3) ◽  
pp. 438-440 ◽  
Author(s):  
Tadashi Terada ◽  
Kanenori Endo ◽  
Hiroyuki Maeta ◽  
Satoshi Horie ◽  
Tetsuo Ohta
Keyword(s):  
Tumor Cells ◽  
Biopsy Specimen ◽  
Smooth Muscle Actin ◽  
Cell Types ◽  
Giant Cells ◽  
Muscle Actin ◽  
Immunohistochemical Examination ◽  
First Case ◽  
Old Japanese ◽  
Giant Tumor

Abstract We report a rare case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells. A 71-year-old Japanese man was admitted to a hospital with melena. Results of a colonoscopy test revealed a polypoid tumor in the rectum, and a biopsy specimen from the lesion showed a sarcoma; the patient underwent rectosigmoidectomy. At gross inspection, the tumor measured 8 × 7 × 4 cm and was polypoid with ulcerations. Necrotic and hemorrhagic foci were scattered. Microscopically, the tumor consisted of 2 cell types: malignant tumor cells with epithelioid features and benign-appearing osteoclast-like giant cells. The tumor cells were polygonal and epithelioid in shape and had eosinophilic or clear cytoplasms, with scattered giant tumor cells. Immunohistochemical examination revealed that the tumor cells were positive for vimentin, muscle actin, α-smooth muscle actin, and desmin, whereas the osteoclast-like giant cells were positive for CD68, leukocyte common antigen, and lysozymes. We diagnosed this case as epithelioid leiomyosarcoma with osteoclast-like giant cells. To the best of our knowledge, this is the first case of rectal epithelioid leiomyosarcoma with osteoclast-like giant cells.

scholarly journals Immunohistochemical Comparison of p53, Ki-67, CD68, Vimentin, α-smooth Muscle Actin and Alpha-1-Antichymotrypsin in Oral Peripheral and Central Giant Cell Granuloma

2015 ◽  
Vol 16 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Omar Kujan ◽  
Ahmad Zahi Al-Shawaf ◽  
Saleh Azzeghaiby ◽  
Ahmad AlManadille ◽  
Kusay Aziz ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Giant Cell ◽  
Mononuclear Cells ◽  
Smooth Muscle Actin ◽  
Giant Cells ◽  
Molecular Techniques ◽  
Muscle Actin ◽  
Giant Cell Granuloma ◽  
Ki 67 ◽  
Central Giant Cell Granuloma

ABSTRACT Introduction Giant cell lesions are characterised histologically by multinucleated giant cells in a background of ovoid to spindleshaped mesenchymal cells. There is a major debate whether these lesions are separate entities or variants of the same disease. Our aim was to study the nature of multinucleated and mononuclear cells from peripheral giant cell granuloma (PGCG), and central giant cell granuloma (CGCG) and giant cell tumor (GCT) of long bones using immunohistochemistry evaluation and to determine whether there is a correlation between recurrence and the markers used. Materials and methods Ki-67, p53, Vimentin, smooth muscle specific actin, CD68 and alpha-1-antichymotrypsin were used to study 60 giant cell lesions. These included 26 CGCG, 28 PGCG, and 6 GCT cases using an avidin-biotin-complex immunohistochemistry standard method. Results All studied cases showed the same results except the percentage of Ki-67 positive mononuclear cells in PGCG was significantly higher than that of both CGCG and GCT (p < 0.05). Interestingly, no statistical correlation between recurrence and the markers used was found. Conclusion Our results may suggest that these lesions have the same histogenesis. The mononuclear stromal cells, both histiocytic and myofibroblastic, are thought to be responsible for the behavior of these lesions whereas the multinucleated cells are considered as reactive. This might support the argument that PGCG, CGCG and GCT are different variants for the same disease. Further studies using molecular techniques are required to elucidate why some of these lesions behave aggressively than others. How to cite this article Kujan O, Al-Shawaf AZ, Azzeghaiby S, AlManadille A, Aziz K, Raheel SA. Immunohistochemical Comparison of p53, Ki-67, CD68, Vimentin, α-smooth Muscle Actin and Alpha-1-Antichymotrypsin in Oral Peripheral and Central Giant Cell Granuloma. J Contemp Dent Pract 2015;16(1):20-24.

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