scholarly journals Antibacterial activity of various fractions of ethyl acetate extract from the desert truffle, Tirmania pinoyi, preliminarily analyzed by gas chromatography-mass spectrometry (GC-MS)

2011 ◽  
Vol 10 (47) ◽  
pp. 9694-9699 ◽  
Author(s):  
Dib Bellahouel ◽  
S ◽  
Fortas ◽  
Z
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Desert Truffle ◽  
Chromatography Mass Spectrometry

scholarly journals GCMS ANALYSIS OF ENDOPHYTIC FUNGI CURVULARIA AERIA MTCC-12847 ISOLATED FROM TRIBULUS TERRESTRIS L.

2019 ◽  
pp. 26-32
Author(s):  
Kamana Sahani ◽  
DEEPENDRA THAKUR
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Active Compound ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Tribulus Terrestris ◽  
Chromatography Mass Spectrometry

Objective: The objective of the present investigation was to perform the Gas Chromatography-mass spectrometry (GCMS) analysis of endophytic fungi Curvularia aeria MTCC-12847 isolated from Tribulus terrestris L. to find out the active compound present in the extract. Methods: The endophytic fungi were isolated from the plant Tribulus Terrestris L., leaf which was cultivated in optimized media for the production of secondary metabolites and was extracted using ethyl acetate. Ethyl acetate extract was used for the Gas Chromatography-mass spectrometry (GCMS) analysis. Results: GC-MS analysis of ethyl acetate extract of endophytic fungi revealed the presence of various secondary metabolites, the highest amount present was Palmitic acid (24.54%) and Lowest was Dimethyl 1-phenyl-7-methyl-1-hydroxy-1,4-dihydronaphthalene-2,3-dicarboxylate (5.76%). Conclusion: The endophytic fungal Curvularia aeria MTCC-12847 extract isolated from the Tribulus terrestris L. shows the presence of various bioactive compounds.

scholarly journals GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sphaeranthus Indicus

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

2018 ◽  
Vol 11 (7) ◽  
pp. 271 ◽  
Author(s):  
Janpen Tangjitjaroenkun
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

scholarly journals ANTIFUNGAL APPRAISAL OF BURKHOLDERIA GLADIOLI STRAIN VIMP03 (JQ867372) AGAINST CERATOCYSTIS PARADOXA

2017 ◽  
Vol 10 (3) ◽  
pp. 221
Author(s):  
Shrikumar Vijaykumar Mahamuni ◽  
Nitin Sadashiv Shinde ◽  
Prakash Vitthal Wani ◽  
Ashok Sitaram Patil
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Culture Filtrate ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Dual Culture ◽  
Chromatography Mass Spectrometry ◽  
Burkholderia Gladioli ◽  
Ceratocystis Paradoxa

ABSTRACTObjective: The purpose of the present investigation was to assess antifungal characterization of Burkholderia gladioli strain VIMP03 (JQ867372), anisolate from sugar beet rhizosphere.Methods: Antifungal characterization was carried out by biochemical, dual culture, and agar well diffusion methods against Ceratocystis paradoxa, asoilborne pathogen of sugarcane and other crops. Culture filtrate and ethyl acetate extract obtained from culture supernatant were analyzed by highperformanceliquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses, respectively.Results: The culture filtrate and ethyl acetate extract exhibited effective antifungal activity. Organic acid profile of the culture filtrate was determined.Acetic acid was mainly produced by the culture under study. The GC-MS analysis of ethyl acetate extract documented the presence of compoundsincluding tetratetracontane, 9-nonadecene, erucic acid, and other hydrocarbon derivatives.Conclusion: The GC-MS, HPLC, and biochemical profiles of B. gladioli strain VIMP03 (JQ867372) revealed its agro clinical-antifungal potential.Keywords: Antifungal, Burkholderia gladioli, High-performance liquid chromatography, Gas chromatography-mass spectrometry.

scholarly journals Uji Aktivitas Antibakteri dari Ekstrak dan Fraksi Daging Buah Mangga Kasturi (Mangifera Casturi) terhadap Bakteri Pseudomonas Aeruginosa dan Bacillus Cereus

2021 ◽  
Vol 49 (2) ◽  
pp. 113-122
Author(s):  
Meliana Meliana ◽  
Sogandi Sogandi ◽  
Ekajayanti Kining
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Inhibitory Concentration ◽  
Mangifera Indica ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry

Kasturi (Mangifera casturi) is a typical plant of South Kalimantan which has only been used for consumption because of its distinctive taste and aroma. Mangifera indica, which is in the same genus as Mangifera casturi, shows antioxidant, antibacterial, antifungal, and antidiarrheal activity, so it is suspected that this plant has the same activity. Crude methanol extract from the meat of Casturi mango was fractionated using n-hexane, ethyl acetate, and methanol-water. Testing for antibacterial activity and minimum inhibitory concentration (MIC) were carried out using the disk diffusion method (Kirby-Bauer). The positive control used was Chloramphenicol because it has an antibacterial range that is rather broad and the negative control used was 10% DMSO. The fraction that had the greatest antibacterial activity was the ethyl acetate fraction; with the formation of a clear zone of 7.98mm against Pseudomonas aeruginosa and 7.52mm against Bacillus cereus, with a minimum inhibitory concentration of 2mg/mL. The next test was a cell leakage analysis which revealed an increase in absorbance at MIC 1 and MIC 2. The Gas Chromatography – Mass Spectrometry (GC-MS) results from the ethyl acetate fraction revealed the presence of phenolic, flavonoid, and glycoide content, with the highest compound concentration of 5-Hydroxymethylfurfural. Keywords: Antibacterial, Extract, Ethyl acetate, fraction, Mangifera casturi Abstrak Kasturi (Mangifera casturi) merupakan tumbuhan khas Kalimantan Selatan yang selama ini hanya dimanfaatkan untuk dikonsumsi karena rasa dan aromanya yang khas. Mangifera indica yang masih satu genus dengan Mangifera casturi menunjukkan aktivitas antioksidan, antibakteri, antijamur, dan antidiare sehingga diduga tanaman ini memiliki aktivitas yang sama. Ekstrak kasar metanol daging buah mangga kasturi difraksinasi dengan menggunakan n-heksan, etil asetat, dan metanol-air. Pengujian aktivitas antibakteri dan kadar hambat minimum dilakukan dengan menggunakan metode difusi cakram (Kirby- Bauer). Kontrol positif yang digunakan adalah Chloramfenikol karena memiliki spektrum antibakteri yang cukup luas dan kontrol negatif yang digunakan adalah DMSO 10%. Fraksi yang memiliki aktivitas antibakteri terbesar pada penelitian ini adalah fraksi etil asetat dengan terbentuknya zona bening sebesar 7,98mm terhadap Pseudomonas aeruginosa dan 7,52mm terhadap Bacillus cereus dengan kadar hambat minimum 2mg/mL. Uji selanjutnya adalah uji kebocoran sel yang menyatakan adanya peningkatan absorbansi pada KHM 1 dan KHM 2. Hasil pemeriksaan Gas Chromatography – Mass Spectrometry (GCMS) dari fraksi etil asetat menyatakan adanya kandungan fenolik, flavonoid, dan glikosida dengan senyawa tertinggi yaitu 5-Hydroxymethylfurfural. Kata kunci: Antibakteri, Ekstrak, Etil asetat, Fraksi, Mangifera castur

scholarly journals Chemical Composition and Antibacterial Activity of Pituranthos Chloranthus Volatile Oil

2007 ◽  
Vol 2 (11) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Mostepha Dahia ◽  
Hocine Laouer ◽  
Adel N. Chaker ◽  
Soizic Prado ◽  
Uwe J. Meierhenrich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

The chemical composition of the essential oil of Pituranthos chloranthus ssp. cossonianus Maire (Apiaceae) was investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) to reveal 54 compounds. The main constituents were myristicine (27.4%), limonene (15.8%), α-pinene (11.4%), and α-phellandrene (8.3%). The antibacterial activity of the oil was evaluated against several strains and was shown to be significant against Pseudomonas aeruginosa.

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