scholarly journals GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sphaeranthus Indicus

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

2018 ◽  
Vol 11 (7) ◽  
pp. 271 ◽  
Author(s):  
Janpen Tangjitjaroenkun
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

scholarly journals GCMS ANALYSIS OF ENDOPHYTIC FUNGI CURVULARIA AERIA MTCC-12847 ISOLATED FROM TRIBULUS TERRESTRIS L.

2019 ◽  
pp. 26-32
Author(s):  
Kamana Sahani ◽  
DEEPENDRA THAKUR
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Active Compound ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Tribulus Terrestris ◽  
Chromatography Mass Spectrometry

Objective: The objective of the present investigation was to perform the Gas Chromatography-mass spectrometry (GCMS) analysis of endophytic fungi Curvularia aeria MTCC-12847 isolated from Tribulus terrestris L. to find out the active compound present in the extract. Methods: The endophytic fungi were isolated from the plant Tribulus Terrestris L., leaf which was cultivated in optimized media for the production of secondary metabolites and was extracted using ethyl acetate. Ethyl acetate extract was used for the Gas Chromatography-mass spectrometry (GCMS) analysis. Results: GC-MS analysis of ethyl acetate extract of endophytic fungi revealed the presence of various secondary metabolites, the highest amount present was Palmitic acid (24.54%) and Lowest was Dimethyl 1-phenyl-7-methyl-1-hydroxy-1,4-dihydronaphthalene-2,3-dicarboxylate (5.76%). Conclusion: The endophytic fungal Curvularia aeria MTCC-12847 extract isolated from the Tribulus terrestris L. shows the presence of various bioactive compounds.

scholarly journals NUTRITIONAL AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ANDROGRAPHIS LAWSONII GAMBLE

2021 ◽  
pp. 47-51
Author(s):  
ELAVARASAN SATHYA ◽  
THANGAVEL SEKAR
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Tamil Nadu ◽  
Methanol Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Nutritional Analysis

Objective: Andrographis lawsonii Gamble is a medicinal herb species, Endemic to India: Karnataka (Dakshina kannada or south Canara district), Kerala (Palakkad district), and Tamil Nadu (Coimbatore and Nilgiris districts). The Andrographis is a large genus of the family Acanthaceae. This study focused to check the preliminary phytochemical nutritive analysis, leaf stem and root and gas chromatography-mass spectrometry (GC-MS) analysis leaf methanol extract. Methods: The plant samples were subjected to Soxhlet extraction for phytochemical analysis and further experimental studies. The test on phytochemical studies indicated the presence of alkaloids, saponins, glycosides, and flavonoids glycosides within the plant parts, respectively. The estimation of carbohydrate protein starch methanol leaves extracts to have high activity compared to others. Results: Preliminary phytochemical and Nutritional analysis in A. lawonii. Nutritional analysis in carbohydrate protein and starch content was found to be high in leaf methanol extract. Highly medicinal compound analysis in this GC-MS Analysis 50 compound present in methanol leaf extract. Conclusion: The leaf methanol extract of A. lawsonii has exhibited remarkable preliminary phytochemical analysis and nutritional analysis GC-MS analysis to the medicinal plant.

BRINE SHRIMPS LETHALITY TEST OF ETHANOL EXTRACT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE FRACTION OF BLIGHIA SAPIDA

2020 ◽  
pp. 208-212
Author(s):  
ADEKOLA MB ◽  
AREOLA JO ◽  
ORIYOMI OV ◽  
APATA JT ◽  
APALOWO OE ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Blighia Sapida

Objective: The objective of the study was to evaluate brine shrimp lethality of Blighia sapida stem-bark extract and its fractions and identify the bioactive constituents in the ethyl acetate fraction (EAF) using gas chromatography-mass spectrometry (GC-MS) technique. Methods: The ethanol extract (EE) and its fractions were subjected to lethality assay, and GC-MS analysis of EAF was carried out. Results: The lethality test showed a concentration-dependent mortality rate in the brine shrimp nauplii for the EE and its fractions. GC-MS analysis of EAF of the extract revealed the existence of 13 peaks of the GC-MS chromatogram with only one prominent compound, n-hexadecanoic acid (peak area of 10.13%). Conclusion: The result revealed the presence of 13 bioactive components in the EAF of the extract, the majority of which have been reported for different biological activities, hence, justifies the use of the plant in the treatment and management of different diseases ethnomedicinally.

scholarly journals ANTIFUNGAL APPRAISAL OF BURKHOLDERIA GLADIOLI STRAIN VIMP03 (JQ867372) AGAINST CERATOCYSTIS PARADOXA

2017 ◽  
Vol 10 (3) ◽  
pp. 221
Author(s):  
Shrikumar Vijaykumar Mahamuni ◽  
Nitin Sadashiv Shinde ◽  
Prakash Vitthal Wani ◽  
Ashok Sitaram Patil
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Culture Filtrate ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Dual Culture ◽  
Chromatography Mass Spectrometry ◽  
Burkholderia Gladioli ◽  
Ceratocystis Paradoxa

ABSTRACTObjective: The purpose of the present investigation was to assess antifungal characterization of Burkholderia gladioli strain VIMP03 (JQ867372), anisolate from sugar beet rhizosphere.Methods: Antifungal characterization was carried out by biochemical, dual culture, and agar well diffusion methods against Ceratocystis paradoxa, asoilborne pathogen of sugarcane and other crops. Culture filtrate and ethyl acetate extract obtained from culture supernatant were analyzed by highperformanceliquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses, respectively.Results: The culture filtrate and ethyl acetate extract exhibited effective antifungal activity. Organic acid profile of the culture filtrate was determined.Acetic acid was mainly produced by the culture under study. The GC-MS analysis of ethyl acetate extract documented the presence of compoundsincluding tetratetracontane, 9-nonadecene, erucic acid, and other hydrocarbon derivatives.Conclusion: The GC-MS, HPLC, and biochemical profiles of B. gladioli strain VIMP03 (JQ867372) revealed its agro clinical-antifungal potential.Keywords: Antifungal, Burkholderia gladioli, High-performance liquid chromatography, Gas chromatography-mass spectrometry.

scholarly journals IN VITRO CYTOTOXIC AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY STUDIES ON ORTHOSIPHON STAMINEUS BENTH. (LEAF) AGAINST MCF–7 CELL LINES

2017 ◽  
Vol 10 (3) ◽  
pp. 129
Author(s):  
Renuka Saravanan ◽  
Brindha Pemiah ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Orthosiphon Stamineus ◽  
Diphenyltetrazolium Bromide ◽  
Anti Cancer ◽  
Mcf 7

ABSTRACTObjective: The objective of the study was to determine the anticancer efficacy of Orthosiphon stamineus extract against Michigan Cancer Foundation-7(MCF-7) and its phytochemical analysis through gas chromatography-mass spectrometry (GC-MS).Methods: Different solvents were used for leaf extraction and used for qualitative assay of phytochemicals using standard protocols. Differentconcentration (12.5, 25, 50, 100, and 200 μg/ml) of methanol extract and ethyl acetate extract of O. stamineus leaves were used to assess the in vitrocytotoxic effect using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Further, the ethyl acetate extract was subjected toGC-MS analysis, and the identification of components was based on the National Institute of Standards and Technology library database.Result: Of the hexane, methanol and ethyl acetate extracts, methanol extract found to contain more of phytochemicals followed by ethyl acetate. Theinhibitory concentration 50 for methanol extract and ethyl acetate extract was found be 93.42 μg/ml and 215.3 μg/ml, respectively.GC-MS mass spectrum of ethyl acetate extract revealed the presence of squalene and phytol and antioxidants such as flavones. Sterols compoundssuch as vitamin E, ergosterol, cholesterol, and 3,7,11,15-tetramethyl-2-hexadecen-1-ol and terpenoids were also identified.Conclusion: The data obtained in this work could be useful as a chemical standard in checking the genuineness of this plant source. Data of the resultsfurther depicted that the selected traditional anti-cancer plants could be used not only as an anti-cancer but also as a good antioxidant.Keywords: Anti-cancer, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Orthosiphon stamineus, Michigan cancer fsoundation-7cells.

scholarly journals The Analysis of Bayer Liquor by SPME-GC-MS of Derivatized Organic Poisons

Proceedings ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 101
Author(s):  
Virgil Badescu ◽  
Raluca Senin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bayer Liquor ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Silylating Agent

The aim of this article was the gas chromatography–mass spectrometry (GC-MS) analysis oforganic matter from a residual liquor sample (S.C. Alum S.A., Tulcea), extracted by the solid-phasemicroextraction method (SPMA) and derivatized with N-(tert-butyldimethylsilyl)-Nmethyltrifluoroacetamide(MTBSTFA) as the silylating agent. [...]

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