Evaluation of in vitro inhibition of mycelial growth of Fusarium solani f. sp. piperis by different products in Brazil

The objectives of this study were (1) to evaluate the effect of different salts on the in vitro development of Fusarium solani var. coeruleum, and (2) to evaluate the efficacy of the salts for reducing dry rot severity caused by the pathogen in potato tubers. The study showed that several salts significantly inhibited the mycelial growth of F. solani var. coeruleum. Aluminium acetate, aluminium chloride, sodium benzoate, sodium metabisulfite, potassium sorbate and trisodium phosphate completely inhibited mycelial growth. Exposure of F. solani var. coeruleum conidia to aluminium acetate, potassium sorbate, sodium benzoate, sodium metabisulfite or trisodium phosphate at 0.2 M resulted in 100% mortality of the conidia after 1 h while aluminium chloride and aluminium lactate caused 100% mortality after an exposure of 24 h. In order to evaluate the effect of salts on potato dry rot development, F. solani var. coeruleum-inoculated tubers were treated with the different salts and disease severity was evaluated following an incubation period of 7 d. Among the test salts, only aluminium chloride caused a significant reduction in potato dry rot compared with the control. The study points out the possibility of using aluminium chloride to control potato dry rot.

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Antifungal Activity of Securidaca longepedunculata and Acacia gourmaensis Hydro-ethanolic Extracts against Three Rice Seed-borne Fungi

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2020/v32i230242 ◽

2020 ◽

pp. 21-28

Author(s):

Léon W. Nitiema ◽

Fabrice W. Nikiema ◽

Drissa Sérémé ◽

Pierre A. E. D. Sombié

Keyword(s):

Antifungal Activity ◽

Fusarium Solani ◽

Fusarium Moniliforme ◽

Mycelial Growth ◽

Ethanolic Extract ◽

Curvularia Lunata ◽

Rice Seed ◽

Securidaca Longepedunculata

Aims: This study was undertaken to investigate the antifungal activities of Securidaca longepedunculata and Acacia gourmaensis bark hydro-ethanolic extract against Fusarium solani, Fusarium moniliforme and Curvularia lunata and to evaluate the percentages of germination and infection of infected rice seeds. Methods: Different extract concentrations ranging from 0.25, 0.5 and 1% were tested during 15 days using poisoned food technique method for in vitro antifungal activity against above three fungal strains. The same concentrations of extract were used to evaluate in vivo antifungal activity on rice seeds infected by these three fungal strains. Results: The extract of Securidaca longepedunculata had antifungal effect on Fusarium solani and Fusarium moniliforme and completely inhibited its mycelial growth at all tested concentrations (0.25, 0.5 and 1%). Curvularia lunata mycelial growth was inhibited of 84.7% by 1% Securidaca longepedunculata extract after five days of culture. However, mycelial growth of F. solani, F. moniliforme and C. lunata were increased with increasing concentration of Acacia gourmaensis extract. It has been observed that when Securidaca longepedunculata hydro-ethanolic extract concentration increased up to 1%, seeds germination percentage decreased for all infection. In contrast, the opposite was observed for Acacia gourmaensis extract. Likewise, seeds infection decreased with the highest concentration (1%) of Securidaca longepedunculata for all infection, whereas the opposite was observed with Acacia gourmaensis extract. Conclusion: This study showed that S. longepedunculata hydro-ethanolic extract has more antifungal activity against seed-borne fungi (F. solani, F. moniliforme and C. lunata) than A. gourmaensis in vitro and in vivo. Results show that Securidaca longepedunculata hydro-ethanolic bark extract can be used as a potential antifungal agent in the management of some rice fungal diseases. Acacia gourmaensis extract, at low concentration, could be used in rice seed treatment to increase seed germination.

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In vitro inhibition of Fusarium solani by Trichoderma harzianum and biofertilizer

Research Society and Development ◽

10.33448/rsd-v10i3.12994 ◽

2021 ◽

Vol 10 (3) ◽

pp. e5210312994

Author(s):

Paula Fernanda de Azevedo ◽

Ana Carolina de Almeida ◽

Rodrigo Domiciano Marques ◽

Christiane Luciana da Costa ◽

Anderson Roberto Benedetti ◽

...

Keyword(s):

Trichoderma Harzianum ◽

Spore Germination ◽

Fusarium Solani ◽

Mycelial Growth ◽

Culture Media ◽

Antagonistic Activity ◽

Dual Culture ◽

Sporulation Rate ◽

Biological Fertilizer

Cassava root rot causes significant production losses. Difficulties of management, along with the lack of chemical fungicides officially registered by the Ministry of Agriculture, Livestock and Supply (MAPA), require alternative control methods. This study investigated the in vitro antagonistic activity of Trichoderma harzianum as well as a biological fertilizer MICROGEO® on Fusarium solani. The phytophatogenic strains of F. solani, called F1 and F2 were isolated from rotted cassava tubers and T. harzianum, strain ESALQ 1306, from a biological fungicide. Continuous liquid composting of bovine ruminal content, water and MICROGEO® produced the biological fertilizer. Dual culture method was used at the bioassay with T. harzianum. Sterilized (St) and unsterilized (USt) biological fertilizer were tested in different concentrations (% v/v) diluted in the culture media. Colony diameters were measured daily in order to establish the mycelial growth velocity index, inhibition percentage, aside from the sporulation rate and spore germination percentage. The mycelial growth of F. solani isolates was interrupted after hyphae encounter with T. harzianum, due to the occurrence of mycoparasitism, but without influence on the sporulation rate. Sterilized biological fertilizer induced no biocontrol, whereas the unsterilized product (concentration 2.5%) inhibited approximately 64% and 85% of the mycelial growth of isolates F1 and F2, respectively. Moreover, spore germination declined with increasing concentration. In conclusion, T. harzianum and the unsterilized biofertilizer showed in vitro antagonistic activity on F. solani.

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Fusarium verticillioides: Evaluation of Fumonisin Production and Effect of Fungicides on In Vitro Inhibition of Mycelial Growth

Mycopathologia ◽

10.1007/s11046-010-9339-9 ◽

2010 ◽

Vol 171 (1) ◽

pp. 77-84 ◽

Cited By ~ 15

Author(s):

Virgínia Carla A. Falcão ◽

Mario Augusto Ono ◽

Tatiana de Ávila Miguel ◽

Edio Vizoni ◽

Elisa Yoko Hirooka ◽

...

Keyword(s):

Mycelial Growth ◽

Fusarium Verticillioides ◽

In Vitro Inhibition

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Integrated management approaches for controlling root rot of bael caused by Fusarium solani

Journal of Applied and Natural Science ◽

10.31018/jans.v8i2.884 ◽

2016 ◽

Vol 8 (2) ◽

pp. 849-854

Author(s):

M. Singh ◽

Sushil Sharma ◽

Mukesh Kumar

Keyword(s):

Fusarium Solani ◽

Root Rot ◽

Mycelial Growth ◽

Management Practices ◽

Integrated Management ◽

Culture Method ◽

Dual Culture ◽

Root Rot Disease ◽

Rot Disease

An experiment was carried out to find out the effective management practices to control the recently recorded pathogen (Fusarium solani) inciting root rot disease in Bael. Rampant incidence due to this pathogen is resulting in excess damage and reduction in acreage. Out of six fungicides screened in vitro, Topsin-M and Bavistin stood at first place in inhibiting the mycelial growth of F. solani. Topsin-M showed 100% inhibition at 50 ppm concentration whereas Bavistin showed 100% inhibition at 150 ppm concentration. Bavistin and Topsin-M as seed dressers effectively protected pre and post emergence seedlings mortality to the tune of 68.75 and 70.95%; 65.00 and 67.54%, respectively. Pre-sowing drenching of soil with Bavistin (0.4%) reduced the pre-emergence mortality from 26.50 to 8.25% and post-emergence mortality from 39.00 to 16.25%. The integration of seed treatment and pre-sowing drenching resulted in 72.51% control of pre emergence mortality and 82.92% control of post emergence mortality. In dual culture method, maximum inhibition of mycelial growth was recorded with Trichoderma harzianum (72.18%) followed by T. viride (67.70%). Glomus mosseae in combination with T. harzianum was found very effective against F. solani under screen house conditions as minimum pre emergence mortality (10.00%) and post emergence mortality (13.25%) against control where the values were 27.25% and 40.25%, respectively. The studies and results compiled here in provide an explanation for the potential of selected fungicides and antagonists in the control of bael root rot disease.

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In Vitro Suppression of Soilborne Plant Pathogens by Coir

HortTechnology ◽

10.21273/hortsci.19.1.96 ◽

2009 ◽

Vol 19 (1) ◽

pp. 96-100 ◽

Cited By ~ 2

Author(s):

Naveen Hyder ◽

James J. Sims ◽

Stephen N. Wegulo

Keyword(s):

Fusarium Solani ◽

Mycelial Growth ◽

Aspergillus Terreus ◽

Plant Pathogens ◽

Cocos Nucifera ◽

Phytophthora Capsici ◽

Potato Dextrose Agar ◽

Soilborne Pathogens ◽

Soilborne Plant Pathogens

Experiments conducted in vitro showed that coir [coconut (Cocos nucifera) mesocarp pith] suppressed growth of soilborne plant pathogens. Mycelial growth of Phytophthora capsici on potato dextrose agar (PDA) amended with an unsterilized coir suspension was strongly inhibited regardless of suspension concentration. Growth of P. capsici on PDA amended with a filter-sterilized coir suspension was uninhibited. Growth of Fusarium solani on water agar (WA) amended with unautoclaved coir was completely inhibited. Growth of F. solani on WA amended with autoclaved coir was uninhibited. Aspergillus terreus recovered from coir inhibited mycelial growth of various soilborne pathogens by up to 75%. The results from this study suggest that coir has the ability to suppress soilborne plant pathogens in vitro and this ability is largely due to microorganisms associated with the substrate.

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Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of Pseudomonas fluorescens and Chitosan

Molecules ◽

10.3390/molecules26216359 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6359

Author(s):

Ariadna Berenice Trejo-Raya ◽

Víctor Manuel Rodríguez-Romero ◽

Silvia Bautista-Baños ◽

Francisco Roberto Quiroz-Figueroa ◽

Ramón Villanueva-Arce ◽

...

Keyword(s):

Fusarium Solani ◽

Mycelial Growth ◽

Higher Plants ◽

Biocontrol Agents ◽

Animal Products ◽

Morphological Alterations ◽

Starting Point ◽

Fungal Phytopathogens ◽

Layer Chromatography

A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of Alternaria alternata and Fusarium solani treated with cell-free extracts of P. fluorescens. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of P. fluorescens contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of P. fluorescens and chitosan [50–1.5% (v/v)] had a synergistic effect since they notably inhibited the mycelial growth of A. altenata and F. solani. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents.

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Influence of Carbon - Nitrogen Supplements and pH on Growth of Sugarcane Stem rot Pathogen Fusarium Solani NVS671

Journal of Fungal Diversity ◽

10.14302/issn.2766-869x.jfd-20-3193 ◽

2020 ◽

Vol 1 (1) ◽

pp. 27-32

Author(s):

Prittesh Patel

Keyword(s):

Fusarium Solani ◽

Mycelial Growth ◽

Growth Promotion ◽

Nitrogen Sources ◽

Cultural Analysis ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

N Sources ◽

Sugarcane Stem

Fusarium solani NVS671 identified from infected sugarcane stem of Co 671 as a new pathogen was subjected to various cultural conditions to understand its physiological profile. In continuation with our previous work, cultural analysis was carried out under in vitro condition by supplementing various carbon and nitrogen sources in Czapek-Dox agar (CDA). Under different hydrogen ion concentrations, it was found that the growth of Fusarium solaniNVS671 was less at pH 4 and pH 10 and could reach up to 5 cm after 7 days of incubation on Potato Dextrose Agar (PDA). It is observed that the pH around 7 to 8 was optimum for the growth of F. solani. Ten different nitrogenous (N) sources and nine different carbon sources were tested on CDA medium to know their effect on the mycelial growth rate and characteristics of the fungus. Among the N sources evaluated, ammonium chloride (7.96±0.11 cm) was found to be most efficient for mycelial growth promotion followed by ammonium nitrate (7.7±0.1 cm) and ammonium sulphate (7.3±0.1 cm). The most preferred carbon source recorded to promote best radial mycelial growth was starch (7.96±0.05 cm) and sucrose (7.93±0.05 cm). Capabilities of using different carbon and nitrogen sources and ability to grow at different pH levels may allow species to adapt to specific soil conditions.This study is important to understand the physiology and metabolite preference of F. solani.

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