Sequencing Analysis of Interleukin-4 gene 590C>T in Patients with Type-2 Diabetes Mellitus

2019 ◽  
Vol 13 (4) ◽  
pp. 321
Author(s):  
Intisar Hussein Ahmed ◽  
Zafir Hassan Ghali
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Interleukin 4 ◽  
Sequencing Analysis

scholarly journals Interleukin-4 −33 C/T Gene Polymorphisms are not Associated with Type 2 Diabetes Mellitus

2020 ◽  
Vol 8 (A) ◽  
pp. 833-836
Author(s):  
Milahayati Daulay ◽  
Mutiara Indah Sari
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Gene Polymorphism ◽  
Gene Polymorphisms ◽  
Interleukin 4 ◽  
Healthy Control ◽  
Different Populations

   BACKGROUND: Various studies have shown variations in the interleukin-4 (IL) gene associated with type 2 diabetes mellitus (T2DM) in different populations and ethnicities. AIM: The objective of this study was to determine the genetic variation of the IL-4 −33 C/T gene in some T2DM patients compared with healthy control in Medan, Sumatera Utara province, Indonesia. METHODS: DNA from T2DM patients and healthy control was isolated, then the determination of the IL-4 −33 C/T gene polymorphism was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: In all samples of this study, IL-4 −33 C/T gene polymorphisms were found in patients with T2DM and healthy control but were not statistically significant. CONCLUSION: The IL-4 −33 C/T gene polymorphism has no relationship with the incidence of T2DM in a portion of our population in Medan, Sumatera Utara province, Indonesia.

scholarly journals Insulin Receptor Substrate-2-dependent Interleukin-4 Signaling in Macrophages Is Impaired in Two Models of Type 2 Diabetes Mellitus

2004 ◽  
Vol 279 (27) ◽  
pp. 28045-28050 ◽  
Author(s):  
Matthew E. Hartman ◽  
Jason C. O'Connor ◽  
Jonathan P. Godbout ◽  
Kyle D. Minor ◽  
Valerie R. Mazzocco ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Insulin Receptor ◽  
Interleukin 4 ◽  
Insulin Receptor Substrate

scholarly journals Interleukin-4 (IL4) -590C/T (rs2243250) gene polymorphism is not associated with diabetic nephropathy (DN) in Caucasians with type 2 diabetes mellitus (T2DM)

2018 ◽  
Vol 18 (4) ◽  
pp. 347-351 ◽  
Author(s):  
Matej Završnik ◽  
Jernej Letonja ◽  
Jana Makuc ◽  
Maja Šeruga ◽  
Ines Cilenšek ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Gene Polymorphism ◽  
Interleukin 1 ◽  
Interleukin 4 ◽  
Diabetic Patients ◽  
Factor Α

Diabetic nephropathy (DN) is a microvascular complication that affects up to 40% of diabetic patients and can lead to end-stage kidney disease. Inflammatory cytokines such as interleukin 1 (IL-1), IL-6, IL-18 and tumor necrosis factor-α (TNFα) have been linked to the development and progression of DN. The aim of our study was to examine the relationship between interleukin-4 (IL4) -590C/T (rs2243250) gene polymorphism and DN in patients with type 2 diabetes mellitus (T2DM). This study is a continuation of our previous research on the association between angiotensinogen (AGT) gene polymorphisms and DN in patients with T2DM. We included 651 unrelated Slovenian (Caucasian) patients who had had T2DM for at least 10 years. The participants were classified into a group of T2DM patients with DN (276 cases) and a group without DN (375 controls). IL4 rs2243250 polymorphism was analyzed using a TaqMan SNP genotyping assay and StepOne Real-Time PCR System. The frequencies of rs2243250 TT, CT and CC (wild type) genotypes were 3.2%, 29.4% and 67.4%, respectively in patients with DN, and 2.7%, 34.4% and 62.9%, respectively in controls. Our logistic regression analysis adjusted for gender, age, diabetes duration, and glycated hemoglobin showed no association between rs2243250 and the risk for DN (OR 1.06; CI 0.37-3.05; p = 0.9). IL4 rs2243250 is not associated with DN in our subset of Slovenian patients with T2DM.

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