Correlation of AgNOR count with biological behavior of N-Nitroso-N-Methylurea (NMU)-induced squamous cell carcinoma in Wistar rats

2015 ◽  
Vol 39 (4) ◽  
pp. 328
Author(s):  
Kaushal Kumar ◽  
K.K. Singh ◽  
M.K. Gupta ◽  
B.K. Roy ◽  
Sanjit Kumar ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Wistar Rats ◽  
Biological Behavior ◽  
Agnor Count

Basaloid Squamous Cell Carcinoma of the Uterine Cervix: Report of a Case With Molecular Analysis

2021 ◽  
pp. 106689692199713
Author(s):  
Jijgee Munkhdelger ◽  
Tomoko Shimooka ◽  
Yoshinori Koyama ◽  
Sadakatsu Ikeda ◽  
Yoshiki Mikami ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Pik3ca Mutation ◽  
Biological Behavior ◽  
Basaloid Squamous Cell Carcinoma ◽  
Surgical Specimen ◽  
Molecular Alterations ◽  
Basaloid Squamous

There is a lack of knowledge about molecular alterations in basaloid squamous cell carcinoma (BSCC) of the uterine cervix. A 72-year-old woman with a history of previous subtotal hysterectomy and current vaginal bleeding was referred to our hospital. Initially, adenoid cystic carcinoma (ACC) was diagnosed upon cervical cytology and biopsy. Chest imaging showed multiple metastatic lesions in both lungs. The surgical specimen showed BSCC with diffuse p16 immunoreactivity and negativity for S-100, c-kit, and neuroendocrine markers. There was a focal minor ACC component, which could have explained the previous cytology and biopsy diagnosis. Next-generation sequencing with two different panels showed coexisting PIK3CA mutation and NTRK2 fusion with 10 additional variants of unknown significance ( ATR, DAXX, FAM123B, JAK1, KEL, MLL2, NOTCH2, PALB2, POLD1, POLE). The MYB gene fusions were not identified. The patient received chemotherapy with TRK inhibitor larotrectinib and carboplatin, which caused shrinkage of metastatic lung nodules. This is the first report of cervical BSCC with extensive molecular workup, which detected multiple genetic events, including targetable ones, which are potentially implicated in the development of a tumor. The accumulation of data and further studies on this tumor are necessary to define its diagnostic criteria and its clinical and biological behavior.

Relationship Between mir15b and Sal-Like Protein 4 and Biological Behavior of Oral Squamous Cell Carcinoma

2021 ◽  
Vol 11 (2) ◽  
pp. 308-314
Author(s):  
Zengbo Wu ◽  
Yan Yan ◽  
Xianzhuo Chen ◽  
Yanling Liu ◽  
Dinggen Chen
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Mucosa ◽  
Clinicopathological Features ◽  
Biological Behavior ◽  
Control Group ◽  
Tumor Tissues ◽  
Proliferation And Invasion

miR15b and SALL4 are involved in a variety of tumor progression. The roles of miR15b and SALL4 in oral squamous cell carcinoma (OSCC) remains unclear. The tumors and normal mucosa of OSCC patients were collected to detect miR15b and SALL4 level by Real-time PCR and analyze their correlation with OSCC clinicopathological features. Oral cancer Tca8113 cells were separated into control group; miR15b mimics group and miR15b inhibitor group followed by analysis of SALL4 expression, cell survival by MTT assay; cell invasion by Transwell chamber assay, as well as expression of N-cadherin and Vimentin and correlated with TNM stage, tumor volume and metastasis, and positively with differentiation TGF-β by Western blot. miR15b expression was decreased and SALL4 expression was increased in OSCC tumor tissues. miR15b was negatively degree (P < 0.05), whereas, opposite correlation of SALL4 with the above parameters was found (P < 0.05). miR15b and SALL4 were negatively correlated. MiR15b mimics significantly up-regulated MiR15b, decreased SALL4 expression, inhibited Tca8113 cell proliferation and invasion, as well as reduced N-cadherin, Vimentin and TGF-βexpression (P < 0.05). Opposite results were found in MiR15b inhibitor group. MiR15b expression is decreased and SALL 4 is increased in OSCC tumor tissues. MiR15b and SALL4 is closely related to OSCC clinicopathological features. MiR15b regulates the expression of EMT-related genes and TGF-β, thereby altering the proliferation and invasion of OSCC cells.

scholarly journals miR-222 regulates the cell biological behavior of oral squamous cell carcinoma by targeting PUMA

2014 ◽  
Vol 31 (3) ◽  
pp. 1255-1262 ◽  
Author(s):  
FANGFANG JIANG ◽  
WEI ZHAO ◽  
LIJIE ZHOU ◽  
LIN ZHANG ◽  
ZIFENG LIU ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior

scholarly journals Gingival Changes in Wistar Rats after Oral Treatment with 4-Nitroquinoline 1-Oxide

2007 ◽  
Vol 01 (03) ◽  
pp. 152-157 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Squamous Cell Carcinoma ◽  
Drinking Water ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Wistar Rats ◽  
Oral Treatment ◽  
Test System ◽  
Gingival Tissue ◽  
Cancer Pathogenesis ◽  
Rat Tongue

ABSTRACTObjectives:4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis is a useful model for studying oral squamous cell carcinoma. However, gingival changes following 4NQO administration via drinking water are absent in the literature. The aim of this study was to investigate gingival changes concomitant to tongue carcinogenesis induced by 4NQO by means of morphological analysis.Methods:Male Wistar rats were distributed into 3 groups of 10 animals each and treated with 50 ppm 4NQO solution by drinking water for 4, 12 or 20 weeks. Thirty animals were used as negative control.Results: Regarding tongue mucosa, the primary histopathological change i.e., hyperplasia and dysplasia was evidenced after 12 weeks treatment with 4NQO. At 20 weeks, squamous cell carcinoma was found in the majority of animals. Gingival squamous hyperplasia was induced by 4NQO after 20-weeks of treatment. Dysplastic changes appeared in some animals (two cases) as well.Conclusions:Taken together, our results support the notion that 4NQO is more effective in rat tongue mucosa than gingival tissue. Probably, this discrepancy depends strongly on route of administration and the susceptibility with respect to animals species. Certainly, such data will contribute when using this experimental test-system for understanding oral cancer pathogenesis. (Eur J Dent 2007;1:152-157)

scholarly journals HLA-DR regulates macrophage phenotypic transformation and affects malignant behavior in esophageal squamous cell carcinoma

2020 ◽  
Author(s):  
Jiang Fen Li ◽  
Yu Fang Xie ◽  
Wei Hua Liang ◽  
Hai Jun Zhang ◽  
Xue Li Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Immune Cell ◽  
Macrophage Polarization ◽  
Biological Behavior ◽  
Invasion And Metastasis ◽  
Hla Dr ◽  
Low Expression

Abstract Background Tumor-associated macrophages (TAMs) are an important immune cell component of the tumor microenvironment. This study aimed to explore the molecular mechanism of TAMs phenotype transformation and the role in the development of esophageal squamous cell carcinoma (ESCC).Methods Co-culture conditions were employed to determine the phenotypic effects of TAMs on ESCC cell biological behavior. Tumor metastasis related molecules VEGF-C and MMP-9 produced by TAMs was evaluated by qRT-PCR and western blot. Expression of HLA-DR was knocked down in TAMs in vitro to determine the effects on macrophage polarization and the biological behavior of ESCC. We determined whether co-injection with M2 TAMs and macrophages depletion affected tumor growth in vivo tumor challenge model. Associations between HLA-DR, TAM density, and clinical outcomes were evaluated in patients with ESCC.Results TAMs in ESCC samples were found to closely reflect the M2 phenotype of TAMs, and exhibited low expression of HLA-DR. Which was involved in ESCC tumor invasion and metastasis. Low expression of HLA-DR positively correlated with high-density of M2 TAMs, indicating high invasiveness and poor prognosis in patients with ESCC. Downregulation of HLA-DR in TAMs led to additional M2-type TAM polarization and more VEGF-C and MMP-9 secretion, promoted the malignant transformation of ESCC.Conclusions These results demonstrate that downregulation of HLA-DR promote the transformation of M2 TAMs, and participate in the invasion and metastasis of ESCC.

scholarly journals Immunoexpression of P16 and Ki 67 in oral squamous cell carcinoma: Association with clinicopathological parameters.

2020 ◽  
Vol 3 (2) ◽  
pp. 135-144
Author(s):  
Shadan Omer ◽  
Payman Rashid
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biological Behavior ◽  
Clinicopathological Parameters ◽  
Statistical Association ◽  
Ki 67 ◽  
Lower Lip ◽  
Significant Statistical Association

Background and Objectives: Oral squamous cell carcinoma (OSCC) is considered as a major health problem worldwide and has been associated with high recurrence rate and poor progno-sis. Advances in understanding of OSCC have not improved the outcome in their management significantly. Many studies have focused on the roles of biomolecular markers in OSCC. The use of p16 and Ki67 as biomarkers of biological behavior of oral squamous cell carcinoma is contro-versial. This study aimed to determine immunoexpression of P16 and Ki67 in oral squamous cell carcinoma and to evaluate their association with various clinicopathological parameters. Materials and Methods: Fifty cases of squamous cell carcinoma from different locations in the oral cavity were included in this cross sectional study. The cases were collected from Rizgary Teaching Hospital and Private Laboratories in Erbil city during a period of eight months from October 2018 to May 2019. The expression of p16 and Ki 67 were evaluated immunohistochem-ically; the findings were correlated with the age of the patients, gender, site of the tumor and grade of the tumor. Result: A total of 50 patients with oral squamous cell carcinoma were enrolled in this study the age ranged from 33 to 89 years, with a mean age ± SD of (64.24 ±12.01) years and more than half (52.0%) of them were males. Lower lip was the most common site of the tumor followed by upper lip and tongue (42.0%, 26.0% and 18.0%, respectively). Histopathological findings of the tumor showed that (54.0%) of the patients had moderately differentiated squamous cell carci-noma. However, (84.0%) of the patients showed negative expression of P 16, while Ki 67 ex-pression was positive among (76.0%) of them. No significant statistical association were found between immunoexpression of p16 and age, sex of patient, site of the tumor and grade of the tumor (P=0.67, P=0,095, P=0.696, P=0.454 respectively). No significant statistical association were found between immunoexpression of Ki67 and age, sex of patient, site of the tumor and grade of the tumor (P=0.637, P=0,411, P=0.353, P=1.00 respectively). Conclusion: in relation to the results obtained in this study no significant association were found between P16 and Ki 67 immunoexpression in oral squamous cell carcinoma with clinicopatho-logical parameters. Further researches have to be designed to better understand the role of p16 and Ki 67 in OSCC. Keywords: oral squamous cell carcinoma, immunoexpression, P16, Ki67.

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