Prevalence ofCitrus tristeza virusin North Eastern region of India and molecular characterization of its isolates

Background: Riemerella anatipestifer (R. anatipestifer) is a gram negative, microaerophilic, non-motile, bipolar bacteria. High genetic diversity and molecular differentiation were reported among field isolates. Although the bacterium causes one of the most economically important duck diseases in the north-eastern region of India, little work has been done on isolation, identification and molecular characterization of the bacteria. Hence, the present investigation was undertaken with a view to characterize the R. anatipestifer isolates from ducks of Assam.Methods: Phenotypic and molecular identification of R. anatipestifer isolates from domesticated ducks of Assam, India were carried out during the period from February, 2019 to January 2020. A total of 624 samples (Ocular swab, throat swab, liver, spleen, kidney, brain, heart, lung) from ducks comprising of apparently healthy, ailing and dead ducks were collected from five districts of Assam, India were processed to isolate and identify the bacteria. The tentative identification of the bacteria was done based on phenotypic characteristics viz., colony morphology, growth characteristics and biochemical reactions. All the phenotypically positive isolates were further subjected to molecular identification based on PCR assay targeting 16S rRNA gene and ERIC sequence.Result: The bacteria could be isolated from different field samples. The highest percentage of the samples that yielded the bacteria are from brain (76%) followed by spleen (74%) of dead ducks and less number of ocular swab (33%) from apparently healthy ducks were found positive. Sequencing of the amplified product of the selected R. anatipestifer isolates targeting 16S rRNA gene revealed homology percentage of 96.5-100%. Further, sequences representing five geographical locations were submitted to NCBI gene bank. Phylogenetic studies of the isolates indicated that there is prevalence of at least two genetically different strains of R. anatipestifer in the study area. The study suggested that the R. anatipestifer infection is endemic in Assam causing varying rate of morbidity (39%) and mortality (53%) and molecular based confirmation is necessary besides phenotypic identification.

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Surveillance and molecular characterization of rotavirus strains circulating in Manipur, North-Eastern India: Increasing prevalence of emerging G12 strains

Infection Genetics and Evolution ◽

10.1016/j.meegid.2010.01.002 ◽

2010 ◽

Vol 10 (2) ◽

pp. 311-320 ◽

Cited By ~ 57

Author(s):

Anupam Mukherjee ◽

Shiladitya Chattopadhyay ◽

Parikshit Bagchi ◽

Dipanjan Dutta ◽

Ng. Brajachand Singh ◽

...

Keyword(s):

Molecular Characterization ◽

Eastern India ◽

North Eastern ◽

North Eastern India

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Polyphasic characterization of phosphate-solubilizing bacteria isolated from rhizospheric soil of the north-eastern region of Portugal

First International Meeting on Microbial Phosphate Solubilization - Developments in Plant and Soil Sciences ◽

10.1007/978-1-4020-5765-6_43 ◽

2007 ◽

pp. 273-276 ◽

Cited By ~ 2

Author(s):

A. Valverde ◽

J. M. Igual ◽

E. Cervantes

Keyword(s):

Eastern Region ◽

Phosphate Solubilizing Bacteria ◽

Rhizospheric Soil ◽

The North ◽

North Eastern ◽

Polyphasic Characterization ◽

Phosphate Solubilizing

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Molecular characterization of rotavirus genotypes circulating in the eastern region of Kenya: Predominance of G9 and emergence of G12 genotypes

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2014.03.935 ◽

2014 ◽

Vol 21 ◽

pp. 247

Author(s):

N. Kiulia ◽

A. Nyachieo

Keyword(s):

Molecular Characterization ◽

Eastern Region

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Occurrences and characterization of endophytic Gluconacetobacter isolated from sugarcane tissues of North Eastern region of India

Indian Journal of Agricultural Research ◽

10.18805/ijare.v0iof.9362 ◽

2016 ◽

Author(s):

Merasenla Ao ◽

Anjuma Gayan ◽

Smirta Buragohain ◽

Dhruba Jyoti Nath

Keyword(s):

Nitrogenase Activity ◽

Carbon Sources ◽

Colony Morphology ◽

Eastern Region ◽

Fresh Tissue ◽

Gram Negative ◽

North Eastern ◽

Intracellular Enzymes ◽

Brown Pigmentation

Sugarcane plants including roots were collected from 21 sugarcane growing locations of Golaghat, Dimapur and Jorhat districts of North Eastern region for isolation of endophytic Gluconacetobacter. Based on the nitrogenase activity, only eight isolates were selected for further characterization of Gluconacetobacter. The nitrogenase activity of the isolates ranged in between 12.4 to 72.4 n mole C2H4 h-1 culture-1. The characteristic yellowish surface pellicles were formed on inoculating the selected isolates into semisolid LGI medium and acetic LGI medium. On enumeration in LGI plates, the frequencies of well recognized Gluconacetobacter like colonies ranged in between 0.75 and 7.68 x 105 (cfu g-1 fresh tissue). Colony morphology of the isolates was irregular flat, irregular raised or circular raised yellow colonies of 1-5 mm in diameter. Light brown to brown pigmentation was observed on potato agar plates and light brown pigmentation with clearing of plates was observed on GYC plates. All the isolates exhibited gram negative and shape of the cells were rods. Reactions to extracellular and intracellular enzymes as well as to the various carbon sources by the isolates explained the diversity among them. The IAR profiles of the eight isolates against twelve antibiotics exhibited wide variation in their response to various antibiotics.

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Spatial and seasonal characterization of terrestrial biospheric carbon flux over India using GOSAT data

ISPRS - International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences ◽

10.5194/isprsarchives-xl-8-617-2014 ◽

2014 ◽

Vol XL-8 ◽

pp. 617-621 ◽

Cited By ~ 2

Author(s):

R. Pradhan ◽

S. K. Goroshi ◽

R. P. Singh

Keyword(s):

Carbon Flux ◽

Co2 Concentration ◽

Spatial And Temporal Variability ◽

Eastern Region ◽

New Era ◽

The North ◽

North Eastern ◽

Flux Measurements ◽

Ct Data

Carbon plays a crucial role in determining the ecosystem balance and slight changes in its concentration in the atmosphere can have significant impacts. The launch of JAXA’s GOSAT (Greenhouse gases Observing SATellite) in 2009 has started a new era of high accuracy CO2 concentration and flux measurements from space borne sensors. This paper reports the spatial and temporal variability of terrestrial biospheric carbon fluxes over the agro-climatic zones of India derived using GOSAT data for the period June 2009 to October 2011. The country averaged biospheric carbon flux varied from −0.47 (October) to 0.37 (April) gC m−2 day−1. Maximum variability in fluxes was observed for the North-Eastern region (−2.18 to +1.38 gC m−2 day−1) whereas the dry region of Rajasthan showed extremely low values (−0.1 to +0.1 gC m−2 day−1). The temporal variation in flux values was compared to averaged NDVI for each zone and indicated that growing season corresponds to more sequestration of carbon from the atmosphere. We compared GOSAT derived biospheric flux with Carbon Tracker (CT) data and observed that the two values show good agreement for all months except June and July. This study provides new estimates of biospheric carbon flux using satellite data driven models to better understand the carbon dynamics associated with terrestrial biosphere over India.

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