Evaluation of the efficacy of benzimidazoles against Ancylostoma caninum by egg hatch assay

2021 ◽  
Vol 35 (1) ◽  
pp. 69-72
Author(s):  
Roja Kurumadas ◽  
Sreedevi Chennuru ◽  
Sudhakar Krovvidi ◽  
Malakondaiah Pattipati
Keyword(s):  
Egg Hatch ◽  
Ancylostoma Caninum ◽  
Egg Hatch Assay

scholarly journals In vitro Efficacy of Albendazole against Strongyle eggs recovered from Trade Goats slaughtered at the Nsukka Abattoir: A Preliminary Survey of Resistance to Albendazole

2021 ◽  
Vol 40 (4) ◽  
pp. 287-295
Author(s):  
I.K. Idika ◽  
V.J. Ebuk ◽  
E.I. Okoro ◽  
T.A. Nzeakor ◽  
N.M. Uzonnah ◽  
...  
Keyword(s):  
Faecal Sample ◽  
Anthelmintic Resistance ◽  
Egg Hatch ◽  
Lc50 Value ◽  
Nematode Parasites ◽  
Faecal Culture ◽  
World Association ◽  
Egg Hatch Assay ◽  
Set Up

The efficacy of Albendazole against trichostrongyle nematode parasites in goats presented for slaughter at the Nsukka municipal  abattoir was evaluated using the In vitro Egg hatch assay (EHA) model. The abattoir was visited once every week for 4 consecutive months during which a total of 240 goats were sampled. Fecal samples were collected per rectum from a minimum of 15 goats on each day of the visit. Egg Hatch Assay was performed on strongyle eggs recovered from pooled faecal sample on each day of sampling with a 2.5% W/V Albendazole. Faecal culture was also set up from the pooled faecal sample on each sampling day to recover and identify the nematode parasites present in the goats. Among the 240 goats sampled, the prevalence of trichostrongylosis as observed by the presence of strongyle eggs was 94.6% (227/240). Faecal culture and larval identification revealed 69.8% of the strongyles as  Haemonchus contortus, while 25.5 and 4.8% were Trichostrongylus colubriformis and Oesophagostomum species respectively. In the EHA, Albendazole had mean LC50 value of 0.16 µg/ml which is slightly in excess of the discriminating dose of 0.1µg/ml as prescribed by the World Association for the Advancement of Veterinary Parasitology (WAAVP) as an indication of anthelmintic resistance. There is therefore an urgent need to screen the nematode parasite population in the Nigeria for the presence Albendazole resistance genes. Key words: GI nematode; egg hatch assay; goat; Albendazole; Resistance; Nigeria

Development of an egg hatch assay for the detection of anthelmintic resistance to albendazole in Fasciola hepatica isolated from sheep

2014 ◽  
Vol 203 (1-2) ◽  
pp. 217-221 ◽  
Author(s):  
D. Robles-Pérez ◽  
J.M. Martínez-Pérez ◽  
F.A. Rojo-Vázquez ◽  
M. Martínez-Valladares
Keyword(s):  
Fasciola Hepatica ◽  
Anthelmintic Resistance ◽  
Egg Hatch ◽  
Egg Hatch Assay

Evaluation of the Egg Hatch Assay and the Larval Migration Inhibition Assay to detect anthelmintic resistance in cattle parasitic nematodes on farms

2012 ◽  
Vol 61 (4) ◽  
pp. 614-618 ◽  
Author(s):  
Janina Demeler ◽  
Nina Kleinschmidt ◽  
Ursula Küttler ◽  
Regine Koopmann ◽  
Georg von Samson-Himmelstjerna
Keyword(s):  
Anthelmintic Resistance ◽  
Parasitic Nematodes ◽  
Inhibition Assay ◽  
Migration Inhibition ◽  
Egg Hatch ◽  
Larval Migration ◽  
Egg Hatch Assay

Variability of the egg hatch assay to survey benzimidazole resistance in nematodes of small ruminants under field conditions

2014 ◽  
Vol 203 (1-2) ◽  
pp. 102-113 ◽  
Author(s):  
C. Calvete ◽  
L.M. Ferrer ◽  
D. Lacasta ◽  
R. Calavia ◽  
J.J. Ramos ◽  
...  
Keyword(s):  
Small Ruminants ◽  
Field Conditions ◽  
Egg Hatch ◽  
Benzimidazole Resistance ◽  
Egg Hatch Assay

scholarly journals In Vitro Pharmacodynamics of Benzimidazole and Tetrahydropyrimidine Derivatives in European Bison Gastro-Intestinal Nematodes

2017 ◽  
Vol 74 (2) ◽  
pp. 167
Author(s):  
Laura CĂTANĂ ◽  
Aurora URSACHE ◽  
Alexandra PETCU ◽  
Flavia PETREAN ◽  
Raul CĂTANĂ
Keyword(s):  
Larval Development ◽  
Nematode Population ◽  
In Vitro Tests ◽  
European Bison ◽  
Egg Hatch ◽  
Parasitic Resistance ◽  
Faecal Samples ◽  
Egg Hatch Assay ◽  
Inducing Resistance

The present study was aimed to evaluate the efficacy of anthelmintic agents against intestinal nematodes found in European bison. It was performed between October 2016 and May 2017, using Egg Hatch Assay (EHA) and Larval Development Assay (LDA). The parasites were obtained from faecal samples, harvested from bisons in Romania and Sweden. The efficacy of albendazole (ABZ), mebendazole (MBZ) thiabendazole (TBZ) and pyrantel (PYR) was tested. In EHA, the maximum efficacy was observed in MBZ (EC50 = - 0.227 μg/ml), and then TBZ (EC50 = - 0.2228). ABZ had a weaker result, EC50 being 0.326 μg/ml. All tested benzimidazoles registered hatching percentages below 50%, reflecting the lack of parasitic resistance. MIC obtained in the LDA tests were 0.2144 μg/ml for TBZ, 0.2792 μg/ml for PYR, 0.5429 μg/ml for MBZ, while ABZ came last (MIC = 0.8187 μg/ml). The in vitro tests proved the antiparasitic molecules efficacy against bisons nematode population and a limited risk of inducing resistance phenomena.

Effect of selection pressure for thiabendazole resistance on fitness of Haemonchus contortus in sheep

Parasitology ◽  
1990 ◽  
Vol 100 (2) ◽  
pp. 327-335 ◽  
Author(s):  
N. Maingi ◽  
M. E. Scott ◽  
R. K. Prichard
Keyword(s):  
Haemonchus Contortus ◽  
Selection Pressure ◽  
Egg Hatch ◽  
Worm Recovery ◽  
Per Capita ◽  
Egg Hatch Assay ◽  
Severe Pathology ◽  
Larval Establishment

SUMMARYA Strain ofHaemonchus contortusshowing moderate levels of resistance to thiabendazole (TBZ) (egg hatch assay LC50 2.1 μM) was subjected, in alternating sequence, to 5 in vitro and 4 in vivo selections with TBZ. This resulted in a significant increase in resistance (egg hatch assay LC50 2.8 μM). The isolates obtained after each combined in vitro and in vivo selection were compared in terms of egg hatch assay, establishment, net egg output, per capita egg output and degree of pathology caused in infected hosts. The increase in resistance was accompanied by a significant increase in larval establishment sheep (22 %) and was associated with more severe pathology, higher egg count and worm recovery 3 months post- infection.

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