Secondary metabolite Constituents, Antimicrobial Activity and Gas chromatography-Mass spectroscopy Profile of Bombax buonopozense P. Beauv.(Bombacaceae) Stem bark Extract

2019 ◽  
Vol 11 (2) ◽  
pp. 87
Author(s):  
A.M. Yusuf-Babatunde ◽  
O.T. Osuntokun ◽  
O.O. Ige ◽  
O.O. Solaja
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Secondary Metabolite ◽  
Mass Spectroscopy ◽  
Stem Bark ◽  
Bark Extract ◽  
Stem Bark Extract ◽  
Gas Chromatography Mass Spectroscopy

scholarly journals Antimicrobial activity and phytochemistry of methanolic stem bark extract of Melia azedarach (Linn.)

2020 ◽  
Vol 12 (1) ◽  
pp. 407-411
Author(s):  
M. Ahmed ◽  
A.H. Bukar ◽  
M.Y. Amuda ◽  
A. Babagana
Keyword(s):  
Antimicrobial Activity ◽  
Developing Countries ◽  
Traditional Medicine ◽  
Stem Bark ◽  
Bark Extract ◽  
Melia Azedarach ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Medicinal Uses ◽  
Stem Bark Extract

The use of traditional medicine is increasing and gaining popularity throughout the developed and developing countries. About 80 % of the people in developing countries rely on traditional medicine for primary health care. The present study was carried out to evaluate the phytochemical constituents and antimicrobial activity of the stem bark of Melia azedarach extracted using methanol as solvent. Phytochemical screening revealed that methanolic stem bark extract contain alkaloid, cardiac glycoside, flavonoids, saponins, steroid, tannin and terpenoid. Antimicrobial efficiency were tested against four human pathogens: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus spp. The diameter of zone of inhibition (ZOI) was measured and it was found that the extract showed a maximum zone of inhibition against Gram negative and Gram positive pathogens and the zone of inhibition was found to be concentration dependent. The result obtained in this study may be indication that alcoholic extracts of this plant could be a possible source of obtaining new and effective herbal medicine to treat infections which justify the ethno    medicinal uses of Melia azedarach against infectious diseases agents.  Key words: Melia azedarach, antimicrobial, activity, traditional medicine, extract

scholarly journals Evaluation of antimicrobial activity and phytochemical composition of some fractions of methanol stem bark extract of Diospyros mespiliformis

2018 ◽  
Vol 11 (1) ◽  
pp. 47-51
Author(s):  
H.M.G. Abubaka ◽  
H Usman ◽  
Y Karumi
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Pyogenes ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Bark Extract ◽  
Active Components ◽  
Column Fraction ◽  
Phytochemical Composition ◽  
Stem Bark Extract ◽  
Micro Organisms

The aim of this study was to determine the active phytochemical(s) most probably responsible for microbial inhibitions, following a bioassay guided protocol. Column chromatographic fractions (AG) obtained from n-Butanol partitioned portion of stem bark extract of Diospyros mespiliformis were analyzed for phytochemical composition. These were subjected to antimicrobial activity tests on clinical isolates of Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Klebsiella pneumoniae and Candida albicans. Phytochemical screening conducted on the column fraction E revealed the presence of tannins, free anthraquinones, cardiac glycosides, saponins, terpenoids, and flavonoids. The anti-microbial test results from fractions A, B, F and G, obtained showed no inhibition against most of the micro-organisms tested, while fractions C, D and E showed significant (P<0.05) activities with diameters of inhibition zone of inhibition ranging from 15.00±1.00 mm to 13.00±6.67 mm against Streptococcus pyogenes at all the concentrations tested (50, 12.5, 6.25 mg/ml equivalent to 3, 1.5 and 0.75 mg/disc). The column fraction E showed the highest inhibition zones with broader concentration dependent pattern with MIC at 12.5 mg/ml. The findings from this study showed the presence of bio-active components against Streptococcus pyogenes with relative higher potency in fraction E. Based on this findings, it can be surmised that Fraction E with significant dose-dependent activity is expected to revealed a potent broad-spectrum antimicrobial agent and thus recommended for further purification stages towards compound(s) isolation of a novel antimicrobial agent.Keywords: Antimicrobial, Diospyros mespiliformis, Phytochemical, Potency, Stem Bark

scholarly journals Antimicrobial Study of Oil and Stem Bark Extracts of Pentaclethra Macrophylla Benth on Six Selected Pathogens

2016 ◽  
Vol 3 (1) ◽  
pp. 7-10
Author(s):  
A. M. Yusuf Babatunde ◽  
L. S. Kasim ◽  
Adegbite A. Ayoade
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Haemolytic Streptococcus ◽  
Stem Bark Extract ◽  
Oil Extract

The antimicrobial activity of oil and stem bark extracts of pentaclethra macrophylla benth was determined against staphylococcus aureus, streptococcus pneumonia, Eschericia coli, Haemophilus influenza, α-Haemolytic Streptococcus and Yeast, using hole diffusion method. The results revealed that the most inhibitory zone (25.0mm) was recorded in hexane oil extract against staphylococcus aureus and α-Haemolytic Streptococcus. The minimum inhibitory concentration (MIC) of the oil extract ranges between 7.8125-62.5mg/ml on all the test organisms and aqueous stem bark extract inhibited only Eschericia coli with minimum, inhibitory concentration (MIC) of 62.5mg/ml. However, the oil extract possessed more antimicrobial activity than the stem bark extract. These findings lend more knowledge to the use of pentaclethra macrophylla benth for medicinal purposes.  

scholarly journals Antibacterial Screening and Phytochemical investigation of bark extracts of Acacia jacquemontii Benth.

2010 ◽  
Vol 2 (2) ◽  
pp. 21-26
Author(s):  
K Choudhary ◽  
M Singh ◽  
U Pillai ◽  
N S Shekhawat
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extract ◽  
Antimicrobial Agents ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Pharmaceutical Sciences ◽  
Antibacterial Screening ◽  
Phytochemical Investigation ◽  
Stem Bark Extract

Acacia  jacquemontii was  assessed  for  active  principles  to  ascertain  the  rationale  for  its  use  in  traditional medicine. Preliminary phytochemical screening of the stem bark extracts showed that it possessed the active principles - alkaloids, glycosides, saponins, terpenoids and tannins. The antimicrobial activity of the extracts was assayed against pathogenic strains of Bacillus cereus, Bacillus pumilus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, S. pyrogenes, and Candida albcans using  the agar diffusion method. The plant extract exhibited antimicrobial activity against all the test microorganisms. B. cereus and B. pumilus were the most susceptible to the plant extract while Candida albicans was the most resistant. The minimum inhibitory  concentration  of  the  stem  bark  extract  of  the  plant  ranged  between  30  and  50 mg/ml while  the minimum bactericidal concentration ranged between 35 and 60 mg/ml. A. jacquemontii could be a potential source of antimicrobial agents.   Key words: Antibacterial; Antifungal; Baonli; Medicinal plants.  DOI: 10.3329/sjps.v2i2.2384Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 21-26

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