Effects of varying levels of nitrogen on plant growth, anti-Fusarium oxysporum activity and 3,5-dicaffeoylquinic acid concentration of extracts from hydroponically cultivated Helichrysum cymosum (L.)

Author(s):  
Yonela Matanzima ◽  
Charles P. Laubscher ◽  
Wilfred T. Mabusela ◽  
Felix Nchu
2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Savita Jandaik ◽  
Preeti Thakur ◽  
Vikas Kumar

The present study was conducted to determine antifungal activity of three different concentrations (5, 10, and 15%) of cow urine against three fungal pathogens (Fusarium oxysporum, Rhizoctonia solani, andSclerotium rolfsii) isolated from infected plants of Methi and Bhindi that showed symptoms of damping off and wilting disease by poison food technique. The extent of growth of test fungi in plates poisoned with cow urine was lesser when compared with the control plates. Among these concentrations cow urine at 15% concentration was most effective. When the three fungal organisms were compared, maximum growth suppression was observed inFusarium oxysporum(78.57%) at 15% concentration of cow urine followed byRhizoctonia solani(78.37%) andSclerotium rolfsii(73.84%). Finally we concluded that the cow urine has antifungal activities and the inhibitory activity can be used in the control of fungi. The nutritional effect of cow urine on plant growth was also tested withTrigonella foenum-graecum(Methi) andAbelmoschus esculentus(Bhindi) plants and the chlorophyll and protein content was also estimated.


2013 ◽  
Vol 55 (1) ◽  
pp. 367-376 ◽  
Author(s):  
Maria Warner ◽  
Wojciech Antkowiak

The influence of NPK fertilization on plant growth and roots yield of <i>Gypsophila paniculata</i> and <i>Gypsophila repens</i> was investigated. The nutrients were applied in different proportions. The health state of <i>Gypsophila</i> was also analyzed. The pathogens isolated from plants showing the diseases symptoms represented eight species: <i>Alternaria dianthicola</i> Neergaard; <i>Fusarium avenaceum</i> (Fr.) Sacc.; <i>Fusarium culmorum</i> (W. G.Smith) Sacc.; <i>F.equiseti</i> (Cola) Sacc.; <i>F.oxysporum</i> Schlecht., Snyder et Hansen; <i>F.sambucinum</i> Fuck; <i>Phytophthora</i> sp. de Bary; <i>Rhizoctonia solani</i> K. Fusarium oxysporum and other species of fungi were isolated from <i>Gypsophila paniculata</i>, whilst from Gypsophila repens only <i>Fusarium oxysporum</i> was obtained.


2019 ◽  
Vol 10 (3) ◽  
pp. 393-402
Author(s):  
Jefferson Guato-Molina ◽  
Javier Auhing-Arcos ◽  
Jorge Crespo-Ávila ◽  
Gabriel Esmeraldas-García ◽  
Antonio Mendoza-León ◽  
...  

2020 ◽  
Vol 2020 ◽  
pp. 1-17 ◽  
Author(s):  
Mohammad Sayyar Khan ◽  
Junlian Gao ◽  
Xuqing Chen ◽  
Mingfang Zhang ◽  
Fengping Yang ◽  
...  

Paenibacillus polymyxa is a plant growth-promoting rhizobacterium that has immense potential to be used as an environmentally friendly replacement of chemical fertilizers and pesticides. In the present study, Paenibacillus polymyxa SK1 was isolated from bulbs of Lilium lancifolium. The isolated endophytic strain showed antifungal activities against important plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea, and Fusarium fujikuroi. The highest percentage of growth inhibition, i.e., 66.67 ± 2.23%, was observed for SK1 against Botryosphaeria dothidea followed by 61.19 ± 3.12%, 60.71 ± 3.53%, and 55.54 ± 2.89% against Botrytis cinerea, Fusarium fujikuroi, and Fusarium oxysporum, respectively. The metabolite profiling of ethyl acetate fraction was assessed through the UHPLC-LTQ-IT-MS/MS analysis, and putative identification was done with the aid of the GNPS molecular networking workflow. A total of 29 compounds were putatively identified which included dipeptides, tripeptides, cyclopeptides (cyclo-(Leu-Leu), cyclo(Pro-Phe)), 2-heptyl-3-hydroxy 4-quinolone, 6-oxocativic acid, anhydrobrazilic acid, 1-(5-methoxy-1H-indol-3-yl)-2-piperidin-1-ylethane-1,2-dione, octadecenoic acid, pyochelin, 15-hydroxy-5Z,8Z,11Z, 13E-eicosatetraenoic acid, (Z)-7-[(2R,3S)-3-[(2Z,5E)-Undeca-2,5-dienyl]oxiran-2-yl]hept-5-enoic acid, arginylasparagine, cholic acid, sphinganine, elaidic acid, gossypin, L-carnosine, tetrodotoxin, and ursodiol. The high antifungal activity of SK1 might be attributed to the presence of these bioactive compounds. The isolated strain SK1 showed plant growth-promoting traits such as the production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, nitrogen fixation, and phosphate solubilization. IAA production was strongly correlated with the application of exogenous tryptophan concentrations in the medium. Furthermore, inoculation of SK1 enhanced plant growth of two Lilium varieties, Tresor and White Heaven, under greenhouse condition. In the light of these findings, the P. polymyxa SK1 may be utilized as a source of plant growth promotion and disease control in sustainable agriculture.


HortScience ◽  
2018 ◽  
Vol 53 (9) ◽  
pp. 1340-1346 ◽  
Author(s):  
Xin Zhao ◽  
Qianru Liu ◽  
M. Tatiana Sanchez ◽  
Nicholas S. Dufault

Fusarium wilt of watermelon can be effectively managed by grafting with resistant rootstocks. Excision and regeneration of grafted seedling roots is a common practice among cucurbit-grafting nurseries that has not been thoroughly examined. The objectives of this study were to compare the performance of grafted and nongrafted watermelon plants under both greenhouse and field conditions when inoculated with Fusarium oxysporum f. sp. niveum (FON) race 2, and assess the effect of root excision on growth of grafted plants with Cucurbita moschata and Cucurbita maxima × C. moschata rootstocks. Two greenhouse experiments (Fall 2015 and Spring 2016) and one field trial (Spring 2016) of seedless watermelon ‘Melody’ were conducted in this study. In both greenhouse experiments, inoculated, nongrafted watermelon plants showed a significantly higher percentage of recovered Fusarium spp. colonies (70% to 75%) compared with grafted treatments (0% to 7.5%). Some plant growth measurements, including the longest vine length and aboveground fresh and dry weight, indicated less vigorous growth for nongrafted plants compared with the grafted treatments. Significantly higher percent recovery of Fusarium spp. below the graft union was observed in the grafted plants with root excision and regeneration treatment (3.7%) in contrast to the intact root treatment (0.5%), suggesting that the root excision method may possibly create entry points for FON infections. Overall, the root excision treatment showed little influence on aboveground growth and root characteristics of grafted plants. Yield of grafted watermelon with FON inoculation in the fumigated field trial was significantly higher than that of noninoculated, nongrafted ‘Melody’ (NGM) control as reflected by the increase of fruit number and size. Averaged over all the grafted treatments, the increase in marketable fruit number and weight reached 108.3% and 240.9%, respectively, and the total fruit number and weight increase was at 80.0% and 237.2%, respectively. However, grafted plants also exhibited greater levels of root-knot nematode infestation as indicated by the significantly higher root galling ratings. Results from this study demonstrated that grafting with squash rootstocks can effectively limit FON colonization in seedless watermelon plants, although more research in rootstock selection and testing is needed to optimize the use of grafted plants for improving plant growth and fruit yield.


2018 ◽  
Vol 39 (4) ◽  
pp. 1437
Author(s):  
Mario Gámiz Infante ◽  
Manuel Avilés Guerrero ◽  
Celia Borrero Vega ◽  
Wilian Carlo Demetrio ◽  
Jair Alves Dionísio

Earthworms are soil invertebrates that play an important environmental role and are often referred to as “ecosystem engineers”. These invertebrates can influence several organisms, from microscopic life forms to plants. Although many works had reported positive effects of earthworms on plant growth, studies combining these invertebrates and soil pathogens showed numerous positive interactions. Fusarium wilt is a global disease that can cause severe damage to strawberry fields. The aim of this study was to evaluate the effects of inoculation of earthworms (Lumbricus terrestris) and Fusarium wilt (Fusarium oxysporum f. sp. fragariae) in strawberry plants. This greenhouse experiment was carried out in the University of Seville School of Agricultural Engineering, Utrera City, Spain. Strawberries (Rooted cuttings) were planted in plastic pots and administered the following treatments: Control (absence of F. oxysporum f. sp. fragariae and earthworms), T1 (absence of F. oxysporum f. sp. fragariae, two L. terrestris per pot), T2 (inoculum of F. oxysporum f. sp. fragariae, absence of L. terrestris), and T3 (inoculum of F. oxysporum f. sp. fragariae and two L. terrestris). Weekly fruit production was measured for seven months. After this period the shoot fresh weight and the leaf nutrient content was measured. The results obtained showed no interaction between L. terrestris and F. oxysporum f. sp. fragariae on strawberry production. T1 treatment resulted in fruit production superior to other treatments, including the control. An absence of differences in dry shoot matter was observed with earthworm treatment, and small differences were found in the leaf nutrient content. The earthworm inoculation was unable to suppress the negative effects of Fusarium wilt in strawberry production. However, positive effects such as a reduction in the disease severity were found in the earthworm treated plants. In treatments without F. oxysporum f. sp. fragariae inoculation, the presence of earthworms increased plant productivity by 44.21 g per pot, compared with the control.


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