The anti-nutritional effect of phytates - the extraphosphorus effect of phytase (review)

An analytical review was conducted on the availability of phosphorus from phytates, which increase the inclusion of phytase in the feed, while not only phytic acid is broken down, but also as a result of a decrease in concentration reduces its anti-nutritional effect.Phosphorus from plant feeds is not fully available to animals, as it is part of phytates, the cleavage of which in the gastrointestinal tract (gastrointestinal tract) of animals is limited. Phytates, getting into the acidic environment of the stomach, ionize and react with positively charged minerals, proteins, amino acids, creating compounds inaccessible for further digestion. The inclusion of phytase in compound feed is accompanied by an extra phosphoric effect, which is expressed in an increase in the availability of amino acids and energy. The decision on the feasibility of including phytase in feed is made on the basis of production tests of the proposed drugs.The studies carried out by the authors of invitro confirm the assumption that phytates enter into chemical interactions with peptides, as well as amino acids formed in the processes of protein digestion, turning them into compounds inaccessible to absorption. The proposed explanation does not affect the results of the balance experiment, but allows us to develop the study of the mechanism in terms of the interaction of digested amino acids with phytates. Based on this, another conclusion can be seen: the destruction of phytates should occur in the body before the digestion and dissolution of protein. In birds, this place is the goiter and stomachs, in pigs - the stomach. The hydrolysis of newly formed phytates (FC-amino acid) requires phytases that are active in the environment of the small intestine at pH 6 -7. As a result, the bound amino acids will be re-released and available for absorption. The development of new phytase preparations, taking into account the proposed explanation, will make it possible to create more effective feed preparations.

Productivity of lettuce in greenhouse as affected by humic and fulvic acids application in association to mineral fertilizer

ABSTRACT Humic substances (HS) have proved to be effective in improving the productivity of vegetable crops. We examined the effect of commercial biostimulants rich in humic (HA) and fulvic acids (FA) obtained from leonardite on agronomic efficiency and nutrient content in the leaves of lettuce (Lactuca sativa). A greenhouse experiment was carried out employing 2.5-L pots containing Ultisol samples that were supplied with commercial biostimulants rich in HA and FA, alone and in combination with NPK mineral fertilizer. The application of HS biostimulants associated to mineral fertilization to the lettuce plants provided the greatest values of the studied variables, namely: plant height and diameter, leaf number per plant and root length. The combination of FA biostimulants and mineral fertilization increased the lettuce productivity relative to the inorganic fertilizer alone. The combined application of rich biostimulants FA and HA with NPK fertilizes influenced K and Fe uptake by the plants, and their accumulation in the aerial portion. Our results suggest that HS products potentialize the nutritional effect of the mineral fertilizer, when applied in combination.

PSXIV-21 Performance of hair lambs maintained on stockpiled permanent pasture mixed with Cleome gynandra L. at multiple stocking densities

Stockpiled forage may extend the grazing season for sheep, reducing feeding costs. Additionally, internal parasites are a problem in sheep managed under grazing conditions. Plants high in phenolic compounds have potential to reduce parasite problems. Cleome gynandra is one such plant that has traditionally been used to treat worms in humans. Thirty-six Katahdin × Dorper lambs (38.8 ± 0.7 kg) were used to investigate the effect of stockpiled forages on lamb performance at different stocking densities: (i) 23 lambs/ha (LOW), (ii) 46 lambs/ha (MED), and (iii) 92 lambs/ha (HIGH). To test C. gynandra for anthelmintic properties, lambs were assigned to (i) permanent pastures (PP), and (ii) PP inter-seeded with C. gynandra (CL). The LOW lambs had the highest ADG (83.8 ± 10.5 g/day), MED lambs were intermediate (48.4 ± 11.6 g/day) and HIGH lambs were lowest (11.0 ± 10.6 g/day; P < 0.001). Lamb BCS did not differ between stocking densities or pasture types (P > 0.10). Fecal egg counts did not differ (P > 0.10) between pasture treatments. In conclusion, stockpiled forages may extend the grazing season for yearling lambs in Southern California. The lack of effect on parasite egg counts may be attributed to grazing C. gynandra at mature stage when nutritional and medicinal values were at their lowest. Future research can clarify the economics of grazing stockpiled forages for sheep at different stages of production, and nutritional effect of C. gynandra on carcass quality.

Effectiveness of Chironomid Larvae in Compare to Other Fish Foods on Growth Parameters and Body Protein of Two Economically Important Fishes

With the increasing human population, the demand for healthy, nutritious but cheap food also increasing day by day. The product of aquaculture mainly fish is more efficient to improve global food security. Along with the seafood, the effort is going on for more production of freshwater fish. For that reason, proper growth and sufficient proximate principles in fish are essential with a low-cost but healthy fish diet. Trichogaster fasciata and, Heteropneustes fossilis were fed with fourth instar larvae of Chironomus striatipennis (F1), dry Tubifex (F2), and granular floating type aquarium fish food (F3) respectively to observe the nutritional effect of different fish meal. Fish fed with F1 showed effective food conversion ratio and specific growth rate in comparison to fish fed on F2 and F3 respectively. The average daily gain was 214.3 % and 47.61% respectively in two fish when fed with F1. Percent gain weight increased from 1.12 (7th day) to 15.03 (28th day) in T. fasciata and 1.497 (7th day) to 8.21 (28th day) in H. fossilis when fed on F1. The result also showed that the protein level was increased steadily in both fishes when fed with F1 in comparison to other foods. It may be concluded that live larvae of Chironomus striatipennis were more effective fish food than dry Tubifex and aquarium fish food. It is a natural organic fish food with a negligible wastage used for fish and keeps the culture medium clean and debris-free with an ecofriendly environment.

Physiological responses of inoculated and uninoculated peanuts under saline stress

This work evaluated the effects of water salinity on the physiological indices in inoculated and non-inoculated peanut plants. The study was carried out in a protected environment at the seedling production unit (UPMA) at Campus das Auroras, at the University for International Integration of the Afro-Brazilian Lusophony (UNILAB), Redenção, Ceará. The experimental design used was in a completely randomized (CRD), with treatments in a factorial arrangement, 5x2, referring to the five salinity levels of the irrigation water - CEa: 0.5, 1.5, 3, 4.5, and 6.0 dSm-1, and inoculated and non-inoculated plants with a mix of rhizobia SEMIA 630, lot 0810, and SEMIA 6144, lot 0312, from Bradyrhizobium sp., isolated, with four replications. Recommended fertilization was done for phosphorus (62.5 kg ha-1 of P) and potassium (50 kg ha-1 of K) to supply the nutritional needs of the plants. The nutritional effect caused by symbiosis with Bradyrhizobium sp. favored inoculated plants to present greater tolerance to salt stress. The availability of nitrogen collaborated to increase the efficiency of plant physiological mechanisms. Uninoculated plants, even with a higher amount of chlorophyll and CO2, were not efficient in the photosynthetic rate. Saline stress affected photosynthesis, transpiration, stomatal conductance, internal CO2 concentration, water use efficiency, and chlorophyll; however, with less intensity when inoculated with Bradyrhizobium sp. The increase in salinity on irrigation water increased the leaf temperature.

Liver-Brain Axis in Sporadic Alzheimer’s Disease: Role of Ten Signature Genes in a Mouse model

Aim:: Poor nutritional effect of junk food induces injurious adversities to the liver and brain but still most of the developing nations survives on these diets to compensate for fast-paced lifestyle. Aim of the study is to infer the proteinconnections behind liver-brain axis and identify the role of these proteins in causing neurodegenerative disorders. Background: : Chronic consumption of fructose and fat rich food works as a toxin in body and have the ability to cause negative metabolic shift. Recently a study was published in Annals of Internal Medicine (2019) citing the loss of vision and hearing in a 14-year-old boy whose diet was strictly restricted to fries and junk-food for almost a decade. This puts the entire body on insulin resistance and related co-morbidities and causes simultaneous damaging effects in liver as well brain. This work provides insights into liver-brain axis and explains how liver is involved in brain related disorders. Objective: : In this study transcriptomic data relating to chronic eating of junk-food was analyzed and simultaneous damage that happens in liver and brain was assessed at molecular level. Method:: Transcriptomic study was taken from GEO database and analysed to find out the genes dysregulated in both liver and brain during this metabolic stress. Cytoscapev3.7 was used to decipher the signalling between liver and brain. This connection between both was called as Liver-Brain axis. Results : The results obtained from our study indicates the role of TUBB5-HYOU1-SDF2L1-DECR1-CDH1-EGFR-SKP2- SOD1-IRAK1-FOXO1 gene signature towards the decline of concurrent liver and brain health. Dysregulated levels of these genes are linked to molecular processes like cellular senescence, hypoxia, glutathione synthesis, amino acid modification, increased nitrogen content, synthesis of BCAAs, cholesterol biosynthesis, steroid hormone signalling and VEGF pathway. Conclusion: We strongly advocate that prolonged consumption of junk food is a major culprit in brain related disorders like Alzheimer’s disease and propose that receptors for brain diseases lie outside the brain and aiming them for drug discovery and design may be beneficial in future clinical studies. This study also discusses the connection between NAFLD (nonalcoholic fatty liver disease) and sAD (sporadic Alzheimer’s disease) owing to liver-brain axis.

A Comparative Study on Breast Milk Feeding and Formula Milk Feeding in Infants With Congenital Heart Disease After Surgery: A Retrospective Study

Objective: To explore the effects of breast milk feeding and formula milk feeding on infants after cardiac surgery in the cardiac intensive care unit (ICU). Methods: Infants who underwent cardiac surgery in our ICU were divided into two groups, according to feeding type. Breast milk feeding and formula milk feeding were separately implemented in the two groups, and the remaining treatment regimens were the same. The related clinical data and feeding effects were recorded and compared. Results: The prealbumin (147.3 ± 15.2 versus 121.5 ± 18.3mg/L) and albumin (46.4 ± 4.2 versus 40.5 ± 5.1 g/L) levels in the breast milk feeding group were better than those in the formula milk feeding group (P < .05). Infants in the breast milk feeding group achieved a better total enteral nutrition time (3.0 ± 1.2 versus 5.2 ± 2.1 d), average daily weight gain (19.0 ± 3.4 versus 14.4 ± 2.3 g/kg·d), length of ICU stay (6.0 ± 2.2 versus 8.1 ± 2.9 d) and length of hospital stay (13.9 ± 4.2 versus 17.8 ± 5.6 d) than those in the formula milk feeding group (P < .05). The incidence of complications such as feeding intolerance, anemia, dyspeptic diarrhea, and nosocomial infection was lower in the breast milk feeding group than in the formula milk feeding group (P < 0.05). Conclusion: Breast milk feeding has a definite nutritional effect on infants after cardiac surgery. It is better than formula milk feeding, making it worthy of popularization and application.

Addition of Anionic Polysaccharide Stabilizers Modulates In Vitro Digestive Proteolysis of a Chocolate Milk Drink in Adults and Children

There is a need to better understand the possible anti-nutritional effect of food stabilizers on the digestibility of important macronutrients, like proteins. This study hypothesized that the anionic nature of κ-, ι-, λ-, Carrageenan (CGN) and xanthan gum directs their interactions with food proteins leading to their subsequent attenuated digestive proteolysis. Model chocolate milk drinks were tested for their colloidal properties, viscosity and proteolytic breakdown in adults and children using in vitro digestion models coupled with proteomic analyses. SDS-PAGE analyses of gastro-intestinal effluents highlight stabilizers hinder protein breakdown in adults and children. Zeta potential and colloidal particle size were the strongest determinants of stabilizers’ ability to hinder proteolysis. LC-MS proteomic analyses revealed stabilizer addition significantly reduced bioaccessibility of milk-derived bioactive peptides with differences in liberated peptide sequences arising mainly from their location on the outer rim of the protein structures. Further, liberation of bioactive peptides emptying from a child stomach into the intestine were most affected by the presence of ι-CGN. Overall, this study raises the notion that stabilizer charge and other properties of edible proteins are detrimental to the ability of humans to utilize the nutritional potential of such formulations. This could help food professionals and regulatory agencies carefully consider the use of anionic stabilizers in products aiming to serve as protein sources for children and other liable populations.

Phytochemical Composition and Antioxidant Activity of Trichoderma reesei Degraded Date (Phoenix dactylifera L.) Pits

Background: Date palm (Phoenix dactylifera L.) is a dominant fruit crop in most of the Arabian countries. Date pits, as a major byproduct which remained after consumption of date flesh proved to be valuable source of energy. Methods: Solid State Degradation (SSD) system was used for the preparation of degraded date pits. Date pits degraded with Trichoderma reesei were evaluated for their proximate, chemical composition and antioxidant activity. Results: The crude fiber content of degraded date pits was found to be 20.8 %, ash (2.09%), crude fat (7.2%), protein (5.56%), and total carbohydrate (87.2%). Neutral detergent fiber (NDF) and acid detergent fiber (ADF) was found to be 74.6% and 45.7%, respectively. Monosaccharide composition of fiber showed that the degradation with T. reesei significantly (P<0.05) enhanced the glucose and mannose content of cellulose, hemicellulose and lignin. Pectin, total carbohydrate and mannan oligosaccharide content were also increased in degraded date pits in which galactose and mannose were the major neutral sugars. Among the studied minerals, potassium, calcium, magnesium, sulphur and phosphorus were predominant. The phenolic and flavonoid contents of degraded date pits significantly (P<0.05) increased to 14.230 g Gallic equivalent/100g DW and 11.68 g Rutin equivalent /100g DW. Degraded date pits also showed significant antioxidant activity as evidenced by the results of 2,2-diphenyl-1- picrylhydrazyl(DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline 6 sulphonicacid) (ABTS) and Ferric Reducing Antioxidant Power (FRAP) assays. Conclusion: Biological degradation with T. reesei significantly (P<0.05) improved the nutritional effect of date pits and thereby its potential usage in food industry was greatly improved.

Review of Structural Features and Binding Capacity of Polyphenols to Gluten Proteins and Peptides In Vitro: Relevance to Celiac Disease

Polyphenols have been extensively studied due to their beneficial effects on human health, particularly for the prevention and treatment of diseases related to oxidative stress. Nevertheless, they are also known to have an anti-nutritional effect in relation to protein metabolism. This effect is a consequence of its binding to digestive enzymes and/or protein substrates. Dietary gluten is the main trigger of celiac disease, a common immune-based disease of the small intestine and for which the only treatment available is the adherence to a gluten-free diet. Recent studies have addressed the use of dietary polyphenols to interact with gluten proteins and avoid its downstream deleterious effects, taking the advantage of the anti-nutritive nature of polyphenols by protein sequestering. Flavonoids, coumarins and tannins have shown the ability to form insoluble complexes with gluten proteins. One of the most promising molecules has been epigallocatechin-3-gallate, which through its binding to gliadins, was able to reduce gliadins digestibility and its ability to stimulate monolayer permeability and transepithelial transport of immunodominant peptides in cell models. This review focuses on the structural features and binding capacity of polyphenols to gluten proteins and peptides, and the prospects of developing an adjuvant therapy in celiac disease.