scholarly journals Burden of Dengue and Chikungunya – A Retrospective Study

2021 ◽  
Author(s):  
J.V. Sathish ◽  
Mita D. Wadekar ◽  
S. Jayashree ◽  
C. Pooja
Keyword(s):  
Retrospective Study ◽  
Early Diagnosis ◽  
Dengue Infection ◽  
Control Measures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
And Control

Arboviral infections like dengue fever and chikungunya are the most common infections that share the same Aedes mosquito vectors. Clinical presentations of these two infections are also similar, especially in initial stages. Non-structural antigen (NS1 Ag)detection for dengue and detection of IgM antibodies by capture ELISA for chikungunya and dengue infection may help in the early diagnosis. Early diagnosis is essential for the treatment and control measures. The present study was conducted to know the burden of dengue and chikungunya. A retrospective study was conducted for a period of 1 year from Dec 2017 to Nov 2018 to know the burden of dengue and chikungunya in Chamarajanagar. Dengue (> 5 days fever) and chikungunya testing was done by IgM antibody capture ELISA kits produced by NIV. Dengue samples (< 5 days fever) were subjected to NS1 antigen detection by microwell enzyme-linked immunosorbent assay (ELISA) from Qualpro diagnostics. The tests were carried out following manufacturer’s instruction. Samples received for dengue NS1 Ag testing was 446, of which, 49(11.0%) were positive and of 730 samples received for IgM antibody, 53 (7.3%) were positive. Age group commonly affected was 0-20 years 44(43.1%). Of 668 samples received for chikungunya test, 86 (12.9%) were positive. Maximum number of cases was seen in age group of 21-40 years 45(52.3%). Males 56(54.9%) were affected higher than female 46(45.1%) in dengue infection while in chikungunya, females 45(52.3%) were more affected than males 41(47.7%). Both infections are high in the month of June and July. Early detection of dengue by NS1 antigen and detection of Ig M antibodies by capture ELISA chikungunya and dengue infection helps in appropriate treatment and initiation of prevention and control measures by community awareness and vector control.

scholarly journals Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

2012 ◽  
Vol 19 (5) ◽  
pp. 804-810 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Robert V. Gibbons ◽  
Ampai Tanganuchitcharnchai ◽  
Mammen P. Mammen ◽  
...  
Keyword(s):  
South Korea ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Virus Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Capture Elisa ◽  
Igm Antibody ◽  
Antigen Elisa ◽  
Ns1 Antigen ◽  
Elisa Format

ABSTRACTSeven commercial assays were evaluated to determine their suitability for the diagnosis of acute dengue infection: (i) the Panbio dengue virus Pan-E NS1 early enzyme-linked immunosorbent assay (ELISA), second generation (Alere, Australia); (ii) the Panbio dengue virus IgM capture ELISA (Alere, Australia); (iii) the Panbio dengue virus IgG capture ELISA (Alere, Australia); (iv) the Standard Diagnostics dengue virus NS1 antigen ELISA (Standard Diagnostics, South Korea); (v) the Standard Diagnostics dengue virus IgM ELISA (Standard Diagnostics, South Korea); (vi) the Standard Diagnostics dengue virus IgG ELISA (Standard Diagnostics, South Korea); and (vii) the Platelia NS1 antigen ELISA (Bio-Rad, France). Samples from 239 Thai patients confirmed to be dengue virus positive and 98 Sri Lankan patients negative for dengue virus infection were tested. The sensitivities and specificities of the NS1 antigen ELISAs ranged from 45 to 57% and 93 to 100% and those of the IgM antibody ELISAs ranged from 85 to 89% and 88 to 100%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics ELISAs gave the best compromise between sensitivity and specificity (87 and 96%, respectively), as well as providing the best sensitivity for patients presenting at different times after fever onset. The Panbio IgG capture ELISA correctly classified 67% of secondary dengue infection cases. This study provides strong evidence of the value of combining dengue virus antigen- and antibody-based test results in the ELISA format for the diagnosis of acute dengue infection.

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection

2018 ◽  
Vol 6 (3) ◽  
pp. 812 ◽  
Author(s):  
Suchita Vikas Ingale ◽  
Aditi Jayant Upadhye ◽  
Jayshree Jayant Upadhye
Keyword(s):  
Platelet Count ◽  
Early Diagnosis ◽  
Indian Subcontinent ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Supportive Therapy ◽  
Hemorrhagic Fever ◽  
Control Measures ◽  
Serum Samples ◽  
Ns1 Antigen

Background: Dengue is an acute viral infection with fatal complications. To reduce the disease burden, early and specific diagnosis of dengue hemorrhagic fever or dengue shock syndrome with supportive therapy reduces morbidity and mortality. This study was undertaken to evaluate the efficacy of NS1 antigen and platelet count.Methods: A total of 200 serum samples were collected from clinically suspected cases of dengue fever. Serum samples were tested for NS1, IgM and IgG and correlated with the platelet count.Results: Out of 200 samples screened in our study, 100 (50%) were positive for dengue parameters, 100 (50%) were negative. Out of 100 positive cases, 95 (95%) cases were positive for NS1antigen either alone or in combination with antibodies. 84 (84%) cases were exclusively positive for NS1 antigen only. Primary infection (positive for NS1 Ag, IgM, NS1 + IgM) was seen in 99 (99%) cases and secondary infection (positive for IgG, NS1 + IgG, IgM + IgG, NS1+ IgM+ IgG) was seen in 1 case (1%). Out of 100 positive cases, thrombocytopenia was observed in 60 (60%) of patients while 40 (40%) had platelet count within normal range.Conclusions: Dengue is endemic to Indian subcontinent. Currently no specific antiviral therapy is available. Measuring platelet count and detecting the NS1 antigen plays a vital role in early diagnosis, management and implementing the control measures in community to avoid spread of dengue.

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals NS1 antigen: A new beam of light in the early diagnosis of dengue infection

2016 ◽  
Vol 9 (12) ◽  
pp. 1212-1214 ◽  
Author(s):  
Muhammad Suleman ◽  
Rani Faryal ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
Shahzad Shaukat ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Dengue Infection ◽  
Ns1 Antigen ◽  
Antigen A

scholarly journals Detection of NS1 antigen, IgM and IgG antibodies using a commercial Dengue rapid test kit for the diagnosis of dengue infection in patients with acute febrile illness

2021 ◽  
Vol 8 (3) ◽  
pp. 235-238
Author(s):  
Nalamanda Suma ◽  
T Sarada
Keyword(s):  
Solid Phase ◽  
Serological Test ◽  
Dengue Infection ◽  
Rapid Test ◽  
Febrile Illness ◽  
Acute Febrile Illness ◽  
Igg Antibodies ◽  
Igm Antibody ◽  
Test Kit ◽  
Ns1 Antigen

Dengue is an endemic arboviral illness. With the increasing incidence of dengue infection, an early diagnostic confirmation of dengue infection in patients facilitates timely clinical intervention, etiological investigation, and disease control. Objective of this study was to evaluate a commercially available serological test kit - Dengue Day 1 Test. This is for the detection of dengue NS1 antigen, differential detection of IgM and IgG antibodies on a single acute serum sample. Atotal of 100 patients with acute febrile illness were included in this study. Serum samples were analysed for Dengue NS1 Ag, IgM & IgG Antibodies using a commercially available Dengue Day 1 Test, rapid solid phase immuno-chromatographic test.As many as 23 (23%) samples were NS1 positive and 17 (17%) samples were positive for IgM antibodies. Based on the combination of dengue NS1 antigen and IgM antibody test, total of 34 patients (34%) were positive for dengue virus infection.Results of the study suggested that a combination of dengue NS1 antigen and IgM antibody tests would increase the rate of detection of dengue illness. This combination would increase the efficacy and aid in early diagnosis of dengue infection.

scholarly journals Seroprevalence of Neospora caninum infection in stray dogs in Chalkidiki, Northern Greece

2021 ◽  
Vol 71 (4) ◽  
pp. 2511
Author(s):  
M. LEFKADITIS ◽  
K. SPANOUDIS ◽  
M. TSAKIROGLOU ◽  
A. PANORIAS ◽  
A. SOSSIDOU
Keyword(s):  
Neospora Caninum ◽  
Control Measures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Infection Prevalence ◽  
Igg Antibodies ◽  
Northern Greece ◽  
Caninum Infection ◽  
Stray Dogs ◽  
Domestic Cycle ◽  
And Control

Despite the importance of N. caninum in veterinary medicine, knowledge on the prevalence of this parasitosis in dogs is limited in some countries. The aim of this study was to assess the prevalence of N. caninum infection in stray dogs in Chalkidiki, Northern Greece. This prospective study was conducted between January 2018 and December 2019 in stray dogs aged ≥6 months old. Blood samples were collected by venipuncture before the female and male dogs underwent the spay and castration procedures, respectively. The indirect enzyme-linked immunosorbent assay (ELISA) was used to screen dogs for the presence of IgG antibodies against N. caninum. Of 511 dogs included in this study, 39 (7.63%) were positive for IgG antibodies against N. caninum. Of all the dogs, 221 were males and 290 females, with positive results for IgG antibodies found in 16 (7.24%) males and 23 (7.93%) females. Preventive measures should be developed and implemented to break the domestic cycle between dogs and bovine. We want to highlight the importance of regional reporting of N. caninum infection prevalence in dogs and control measures by veterinarians and veterinary authorities to farmers and public, in order to avoid this disease’s spread.

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